MX2017008627A - Vlps derivadas de virus de planta con dsrna encapsidado y metodo de sintesis. - Google Patents

Vlps derivadas de virus de planta con dsrna encapsidado y metodo de sintesis.

Info

Publication number
MX2017008627A
MX2017008627A MX2017008627A MX2017008627A MX2017008627A MX 2017008627 A MX2017008627 A MX 2017008627A MX 2017008627 A MX2017008627 A MX 2017008627A MX 2017008627 A MX2017008627 A MX 2017008627A MX 2017008627 A MX2017008627 A MX 2017008627A
Authority
MX
Mexico
Prior art keywords
dsrna
virus
plant virus
encapsidated
vlps
Prior art date
Application number
MX2017008627A
Other languages
English (en)
Inventor
Darío Cadena Nava Rubén
NUÑEZ RIVERA Alfredo
Norberto Zamudio Ocadiz José
Original Assignee
Univ Mexico Nac Autonoma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Mexico Nac Autonoma filed Critical Univ Mexico Nac Autonoma
Priority to MX2017008627A priority Critical patent/MX2017008627A/es
Priority to PCT/MX2018/000060 priority patent/WO2019004814A1/es
Publication of MX2017008627A publication Critical patent/MX2017008627A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se describen partículas tipo virus con dsRNA encapsidado que presentan una estructura con morfologías esféricas y nanotubulares, y que se componen de una cápside formada por proteínas estructurales de un virus que infecta plantas y un dsRNA de doble cadena de entre 20 y 10´000 pares de bases de longitud. Se describe su uso como vector para el transporte de dsRNA a células blanco con la finalidad de bloquear la expresión de genes. Se describe además el método in vitro de encapsidación del dsRNA de longitud variable dentro de cápsides de virus de plantas (VLPs) compuesto por tres etapas: de purificación, ensamblaje y estabilización.
MX2017008627A 2017-06-28 2017-06-28 Vlps derivadas de virus de planta con dsrna encapsidado y metodo de sintesis. MX2017008627A (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MX2017008627A MX2017008627A (es) 2017-06-28 2017-06-28 Vlps derivadas de virus de planta con dsrna encapsidado y metodo de sintesis.
PCT/MX2018/000060 WO2019004814A1 (es) 2017-06-28 2018-06-26 Vlps derivadas de virus de planta con dsrna encapsidado y método de sintesis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MX2017008627A MX2017008627A (es) 2017-06-28 2017-06-28 Vlps derivadas de virus de planta con dsrna encapsidado y metodo de sintesis.

Publications (1)

Publication Number Publication Date
MX2017008627A true MX2017008627A (es) 2019-02-08

Family

ID=64741728

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017008627A MX2017008627A (es) 2017-06-28 2017-06-28 Vlps derivadas de virus de planta con dsrna encapsidado y metodo de sintesis.

Country Status (2)

Country Link
MX (1) MX2017008627A (es)
WO (1) WO2019004814A1 (es)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010047839A1 (en) * 2008-10-25 2010-04-29 Aura Biosciences Modified plant virus particles and uses therefor
BR112013029143A2 (pt) * 2011-05-13 2017-07-11 Folia Biotech Inc processo in vitro para o preparo de partículas similares a vírus e seu uso, partícula similar a vírus de vírus do mosaico do mamoeiro, composição farmacêutica e método para acentuar uma resposta imune a um antígeno e para estimular a resposta imune inata em um indivíduo
US9605031B2 (en) * 2013-11-19 2017-03-28 The Regents Of The University Of California In vitro reconstituted plant virus capsids for delivering RNA genes to mammalian cells

Also Published As

Publication number Publication date
WO2019004814A1 (es) 2019-01-03

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