MX2012003626A - Metodo de deteccion de moleculas por espectrometria de masa. - Google Patents
Metodo de deteccion de moleculas por espectrometria de masa.Info
- Publication number
- MX2012003626A MX2012003626A MX2012003626A MX2012003626A MX2012003626A MX 2012003626 A MX2012003626 A MX 2012003626A MX 2012003626 A MX2012003626 A MX 2012003626A MX 2012003626 A MX2012003626 A MX 2012003626A MX 2012003626 A MX2012003626 A MX 2012003626A
- Authority
- MX
- Mexico
- Prior art keywords
- ions
- ejected
- characteristic
- trapped
- target molecule
- Prior art date
Links
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/26—Mass spectrometers or separator tubes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/0027—Methods for using particle spectrometers
- H01J49/0031—Step by step routines describing the use of the apparatus
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/26—Mass spectrometers or separator tubes
- H01J49/34—Dynamic spectrometers
- H01J49/42—Stability-of-path spectrometers, e.g. monopole, quadrupole, multipole, farvitrons
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/26—Mass spectrometers or separator tubes
- H01J49/34—Dynamic spectrometers
- H01J49/42—Stability-of-path spectrometers, e.g. monopole, quadrupole, multipole, farvitrons
- H01J49/426—Methods for controlling ions
- H01J49/427—Ejection and selection methods
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Electron Tubes For Measurement (AREA)
Abstract
La presente invención se refiere a un método de detección de al menos una molécula objetivo en una muestra a través de espectrometría de masa, en donde a) las moléculas de la muestra son ionizadas; b) las siguientes etapas (i) y (ii) se llevan a cabo n veces, n es igual a 0, 1, 2, 3 ó 4; (i) al menos un ion obtenido en la etapa anterior es seleccionado con base en la molécula objetivo en un analizador de masa y (ii) el ion seleccionado de esta manera es fragmentado en una célula de fragmentación; c) al menos dos diferentes iones obtenidos en la etapa a) cuando n es cero, o en la etapa b) cuando n no es cero, son capturados en un analizador de masa, al menos dos iones atrapados que tienen una relación de masa/carga m/z que es característica de la molécula objetivo; d) los iones característicos atrapados de esta manera son expulsados del analizador de masa, y e) los iones característicos expulsados son detectados por medio de un dispositivo de detección. El método de la invención es característico porque los iones característicos son expulsados al mismo tiempo que la etapa d) y son detectados simultáneamente con la etapa e).
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0956662A FR2950697B1 (fr) | 2009-09-25 | 2009-09-25 | Procede de detection de molecules par spectrometrie de masse |
PCT/FR2010/051919 WO2011036378A1 (fr) | 2009-09-25 | 2010-09-15 | Procede de detection de molecules par spectrometrie de masse |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2012003626A true MX2012003626A (es) | 2012-08-23 |
Family
ID=42105918
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2012003626A MX2012003626A (es) | 2009-09-25 | 2010-09-15 | Metodo de deteccion de moleculas por espectrometria de masa. |
Country Status (11)
Country | Link |
---|---|
US (1) | US9018580B2 (es) |
EP (1) | EP2481078B1 (es) |
JP (1) | JP5730878B2 (es) |
KR (1) | KR20130004561A (es) |
CN (1) | CN102598204A (es) |
BR (1) | BR112012006486A2 (es) |
FR (1) | FR2950697B1 (es) |
IN (1) | IN2012DN02538A (es) |
MX (1) | MX2012003626A (es) |
RU (1) | RU2012113416A (es) |
WO (1) | WO2011036378A1 (es) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2833454C (en) * | 2011-04-21 | 2020-03-10 | Biomerieux Inc. | Method of detecting at least one mechanism of resistance to cephalosporins by mass spectrometry |
US9870911B2 (en) * | 2013-12-23 | 2018-01-16 | Dh Technologies Development Pte. Ltd. | Method and apparatus for processing ions |
WO2016134365A1 (en) * | 2015-02-20 | 2016-08-25 | The Johns Hopkins University | Biomarkers of myocardial injury |
JPWO2016147839A1 (ja) * | 2015-03-17 | 2017-12-28 | アサヒビール株式会社 | ハイドロフォビンの定量方法及び発泡麦芽飲料の噴き性予測方法 |
JP6473366B2 (ja) * | 2015-03-31 | 2019-02-20 | シスメックス株式会社 | 温度判定方法、標的ペプチドの検出方法および温度判定試薬 |
KR102062149B1 (ko) * | 2015-05-22 | 2020-01-03 | 익스프레션 패톨로지, 인크. | 사이클린-의존성 키나제 억제제 2A(p16) 단백질에 대한 SRM/MRM 분석법 |
US11511213B2 (en) | 2015-08-13 | 2022-11-29 | Waters Technologies Corporation | Nickel-cobalt alloy material devices and components |
US10900936B2 (en) * | 2015-10-28 | 2021-01-26 | Shimadzu Corporation | Gas chromatograph and sample injection method |
EP3373324A1 (en) * | 2017-03-10 | 2018-09-12 | Thermo Finnigan LLC | Methods and systems for quantitative mass analysis |
GB201802917D0 (en) | 2018-02-22 | 2018-04-11 | Micromass Ltd | Charge detection mass spectrometry |
US11984311B2 (en) * | 2018-10-10 | 2024-05-14 | Purdue Research Foundation | Mass spectrometry via frequency tagging |
US11842891B2 (en) | 2020-04-09 | 2023-12-12 | Waters Technologies Corporation | Ion detector |
US11232936B2 (en) * | 2020-05-28 | 2022-01-25 | Thermo Finnigan Llc | Absolute quantitation of a target analyte using a mass spectrometer |
KR102625713B1 (ko) | 2021-12-16 | 2024-01-17 | 한국전자기술연구원 | 스마트 안전바 시스템 |
Family Cites Families (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5171991A (en) * | 1991-01-25 | 1992-12-15 | Finnigan Corporation | Quadrupole ion trap mass spectrometer having two axial modulation excitation input frequencies and method of parent and neutral loss scanning |
US5200613A (en) * | 1991-02-28 | 1993-04-06 | Teledyne Mec | Mass spectrometry method using supplemental AC voltage signals |
CA2240325A1 (en) * | 1998-03-27 | 1998-11-14 | Synsorb Biotech, Inc. | Methods for screening compound libraries |
GB2389704B (en) * | 2002-05-17 | 2004-06-02 | * Micromass Limited | Mass Spectrometer |
US20070269895A1 (en) | 2002-06-03 | 2007-11-22 | The Institute For Systems Biology | Methods for quantitative proteome analysis of glycoproteins |
US7501286B2 (en) | 2002-08-14 | 2009-03-10 | President And Fellows Of Harvard College | Absolute quantification of proteins and modified forms thereof by multistage mass spectrometry |
US7632686B2 (en) | 2002-10-03 | 2009-12-15 | Anderson Forschung Group | High sensitivity quantitation of peptides by mass spectrometry |
JP4515819B2 (ja) * | 2003-08-13 | 2010-08-04 | 株式会社日立ハイテクノロジーズ | 質量分析システム |
US7473892B2 (en) * | 2003-08-13 | 2009-01-06 | Hitachi High-Technologies Corporation | Mass spectrometer system |
US6958473B2 (en) * | 2004-03-25 | 2005-10-25 | Predicant Biosciences, Inc. | A-priori biomarker knowledge based mass filtering for enhanced biomarker detection |
JPWO2005098071A1 (ja) | 2004-04-08 | 2008-02-28 | 株式会社 東北テクノアーチ | 水素処理により合金の結晶粒を微細化する方法 |
JP4300154B2 (ja) | 2004-05-14 | 2009-07-22 | 株式会社日立ハイテクノロジーズ | イオントラップ/飛行時間質量分析計およびイオンの精密質量測定方法 |
JP2006300758A (ja) | 2005-04-21 | 2006-11-02 | Apro Life Science Institute Inc | 内部標準ペプチドを用いて生体由来試料に含まれる標的タンパク質を定量する方法 |
EP1736480B9 (en) | 2005-06-02 | 2009-03-04 | Polyquant GmbH | Artificial protein, method for absolute quantification of proteins and uses thereof |
GB0511386D0 (en) * | 2005-06-03 | 2005-07-13 | Shimadzu Res Lab Europe Ltd | Method for introducing ions into an ion trap and an ion storage apparatus |
GB0524042D0 (en) * | 2005-11-25 | 2006-01-04 | Micromass Ltd | Mass spectrometer |
JP5259169B2 (ja) * | 2007-01-10 | 2013-08-07 | 日本電子株式会社 | タンデム型飛行時間型質量分析装置および方法 |
GB0701476D0 (en) * | 2007-01-25 | 2007-03-07 | Micromass Ltd | Mass spectrometer |
GB0703378D0 (en) * | 2007-02-21 | 2007-03-28 | Micromass Ltd | Mass spectrometer |
WO2008146100A1 (en) | 2007-06-01 | 2008-12-04 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Method for absolute quantification of polypeptides |
GB0717146D0 (en) * | 2007-09-04 | 2007-10-17 | Micromass Ltd | Mass spectrometer |
US8399827B1 (en) * | 2007-09-10 | 2013-03-19 | Cedars-Sinai Medical Center | Mass spectrometry systems |
GB2463633B (en) * | 2008-05-15 | 2013-02-27 | Thermo Fisher Scient Bremen | MS/MS data processing |
JP5449701B2 (ja) * | 2008-05-28 | 2014-03-19 | 株式会社日立ハイテクノロジーズ | 質量分析計 |
WO2009146418A1 (en) * | 2008-05-30 | 2009-12-03 | Purdue Research Foundation | Non-destructive, high order harmonic ion motion image current detection |
GB201011862D0 (en) * | 2010-07-14 | 2010-09-01 | Thermo Fisher Scient Bremen | Ion detection arrangement |
-
2009
- 2009-09-25 FR FR0956662A patent/FR2950697B1/fr active Active
-
2010
- 2010-09-15 KR KR1020127010654A patent/KR20130004561A/ko not_active Application Discontinuation
- 2010-09-15 RU RU2012113416/15A patent/RU2012113416A/ru not_active Application Discontinuation
- 2010-09-15 IN IN2538DEN2012 patent/IN2012DN02538A/en unknown
- 2010-09-15 BR BR112012006486A patent/BR112012006486A2/pt not_active IP Right Cessation
- 2010-09-15 EP EP10770566.7A patent/EP2481078B1/fr active Active
- 2010-09-15 WO PCT/FR2010/051919 patent/WO2011036378A1/fr active Application Filing
- 2010-09-15 US US13/496,949 patent/US9018580B2/en active Active
- 2010-09-15 CN CN2010800427280A patent/CN102598204A/zh active Pending
- 2010-09-15 JP JP2012530311A patent/JP5730878B2/ja active Active
- 2010-09-15 MX MX2012003626A patent/MX2012003626A/es not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
RU2012113416A (ru) | 2013-10-27 |
FR2950697A1 (fr) | 2011-04-01 |
BR112012006486A2 (pt) | 2016-11-22 |
CN102598204A (zh) | 2012-07-18 |
EP2481078A1 (fr) | 2012-08-01 |
EP2481078B1 (fr) | 2023-06-21 |
IN2012DN02538A (es) | 2015-08-28 |
JP2013506124A (ja) | 2013-02-21 |
FR2950697B1 (fr) | 2011-12-09 |
US9018580B2 (en) | 2015-04-28 |
KR20130004561A (ko) | 2013-01-11 |
JP5730878B2 (ja) | 2015-06-10 |
US20120205535A1 (en) | 2012-08-16 |
WO2011036378A1 (fr) | 2011-03-31 |
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Legal Events
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FA | Abandonment or withdrawal |