MX2012002902A - Compensacion para diafonia espectral en amplificacion de acido nucleico multiplexado. - Google Patents
Compensacion para diafonia espectral en amplificacion de acido nucleico multiplexado.Info
- Publication number
- MX2012002902A MX2012002902A MX2012002902A MX2012002902A MX2012002902A MX 2012002902 A MX2012002902 A MX 2012002902A MX 2012002902 A MX2012002902 A MX 2012002902A MX 2012002902 A MX2012002902 A MX 2012002902A MX 2012002902 A MX2012002902 A MX 2012002902A
- Authority
- MX
- Mexico
- Prior art keywords
- nucleic acid
- amplification
- acid amplification
- compensation
- spectral
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
- G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Computational Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Theoretical Computer Science (AREA)
- Medical Informatics (AREA)
- Evolutionary Biology (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Algebra (AREA)
- General Physics & Mathematics (AREA)
- Mathematical Analysis (AREA)
- Mathematical Optimization (AREA)
- Mathematical Physics (AREA)
- Pure & Applied Mathematics (AREA)
Abstract
La presente invención se refiere a un método que incluye realizar una ampliación de ácido nucleico de una muestra de ácido nucleico usando una sonda de detección, en donde la amplificación de ácido nucleico ocurre sobre uno o más periodos de interrogación, y, de la amplificación de ácido nucleico, adquirir datos de amplificación que indican una cantidad de ácido nucleico presente en cada uno de uno o más periodos de interrogación. El método también incluye, en base a los datos de amplificación, determinar un valor de corrección de diafonía asociado con un vecino espectral a la sonda para reducir la diafonía espectral del vecino espectral; y aplicar el valor de corrección de diafonía a los datos de amplificación recolectados de amplificaciones de ácido nucleico multiplexados de muestras de ácido nucleico.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24130709P | 2009-09-10 | 2009-09-10 | |
| US35828710P | 2010-06-24 | 2010-06-24 | |
| PCT/US2010/047265 WO2011031585A1 (en) | 2009-09-10 | 2010-08-31 | Compensation for spectral crosstalk in multiplex nucleic acid amplification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2012002902A true MX2012002902A (es) | 2012-04-02 |
| MX352971B MX352971B (es) | 2017-12-15 |
Family
ID=43732754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2012002902A MX352971B (es) | 2009-09-10 | 2010-08-31 | Compensacion para diafonia espectral en amplificacion de acido nucleico multiplexado. |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US9938569B2 (es) |
| EP (3) | EP2475788B1 (es) |
| CN (1) | CN102482720B (es) |
| DK (3) | DK3375891T3 (es) |
| ES (3) | ES2680495T3 (es) |
| MX (1) | MX352971B (es) |
| PT (3) | PT3375891T (es) |
| WO (1) | WO2011031585A1 (es) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2009240461A1 (en) | 2008-04-24 | 2009-10-29 | 3M Innovative Properties Company | Analysis of nucleic acid amplification curves using wavelet transformation |
| PT3375891T (pt) * | 2009-09-10 | 2020-05-11 | Diasorin S P A | Métodos e dispositivos para compensação de diafonia espectral em amplificação de ácido nucleico multiplex |
| KR102145677B1 (ko) | 2012-02-03 | 2020-08-18 | 캘리포니아 인스티튜트 오브 테크놀로지 | 다중화된 생화학적 분석에서의 시그널 인코딩 및 디코딩 |
| KR101969846B1 (ko) | 2012-06-28 | 2019-04-17 | 삼성전자주식회사 | Ｐｃｒ 데이터 및 다른 데이터에서 크로스토크의 영향을 보상하여 핵산을 분석하는 방법 및 장치 |
| CN104662172B (zh) | 2012-08-03 | 2018-07-06 | 加州理工学院 | Pcr中具有减少的硬件和要求的多重化和定量 |
| US8858886B1 (en) | 2013-05-08 | 2014-10-14 | Agilent Technologies, Inc. | Scanning system with interchangeable optical cartridges for fluorescence measurements |
| US9170198B2 (en) | 2013-12-19 | 2015-10-27 | Luminex Corporation | Crosstalk reduction |
| BE1022048B1 (nl) * | 2014-04-11 | 2016-02-10 | Ugentec Bvba | Methoden voor fluorescentie data correctie |
| EP2990490B1 (en) * | 2014-08-27 | 2020-09-23 | F. Hoffmann-La Roche AG | An analysis method and system for analyzing a nucleic acid amplification reaction |
| CN107426044B (zh) * | 2016-05-23 | 2022-06-07 | 中兴通讯股份有限公司 | 一种串线检测方法、装置及操作维护服务器 |
| WO2017218777A1 (en) | 2016-06-17 | 2017-12-21 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
| US10963992B2 (en) * | 2016-10-25 | 2021-03-30 | Qiagen Gmbh | Cross-talk compensation |
| US10623132B2 (en) * | 2018-03-06 | 2020-04-14 | J3 Technology LLC | Barrage jammer with contoured amplitude |
| CN108841939B (zh) * | 2018-06-21 | 2020-09-22 | 北京致雨生物科技有限公司 | 多重数字pcr浓度测量方法及微滴式数字pcr系统 |
| US20230296513A1 (en) * | 2020-07-23 | 2023-09-21 | Life Technologies Corporation | Systems and methods for biological analysis |
| CN111849758A (zh) * | 2020-08-18 | 2020-10-30 | 上海基灵生物科技有限公司 | 全自动实时荧光定量pcr检测系统 |
| CN113174326B (zh) * | 2021-01-15 | 2021-12-10 | 北京中科生仪科技有限公司 | 基于pcr的纯化扩增反应中的控制装置 |
| CN114752663B (zh) * | 2022-05-18 | 2024-04-30 | 苏州国科芯感医疗科技有限公司 | 一种荧光串扰校正方法及其应用 |
| CN117434044B (zh) * | 2023-12-19 | 2024-03-08 | 鲲鹏基因（北京）科技有限责任公司 | 一种pcr仪荧光串扰系数标定方法及装置与应用 |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7133726B1 (en) | 1997-03-28 | 2006-11-07 | Applera Corporation | Thermal cycler for PCR |
| US6303305B1 (en) | 1999-03-30 | 2001-10-16 | Roche Diagnostics, Gmbh | Method for quantification of an analyte |
| US6156465A (en) | 1999-04-12 | 2000-12-05 | Cymbolic Sciences Inc. | Crosstalk correction |
| US6333501B1 (en) | 2000-01-27 | 2001-12-25 | Perkin-Elmer Corporation | Methods, apparatus, and articles of manufacture for performing spectral calibration |
| US6734401B2 (en) | 2000-06-28 | 2004-05-11 | 3M Innovative Properties Company | Enhanced sample processing devices, systems and methods |
| US6627159B1 (en) | 2000-06-28 | 2003-09-30 | 3M Innovative Properties Company | Centrifugal filling of sample processing devices |
| US7188030B2 (en) | 2001-08-21 | 2007-03-06 | Applera Corporation | Automatic threshold setting for quantitative polymerase chain reaction |
| US7435602B2 (en) | 2002-12-20 | 2008-10-14 | Applied Biosystems Inc. | Reducing effects of spectral nonuniformity |
| US7788039B2 (en) * | 2003-09-25 | 2010-08-31 | Roche Molecular Systems, Inc. | Quantitation of nucleic acids using growth curves |
| US7177023B2 (en) | 2004-03-19 | 2007-02-13 | Applera Corporation | Fluorescent light detection |
| US7023451B2 (en) * | 2004-06-14 | 2006-04-04 | Sharp Laboratories Of America, Inc. | System for reducing crosstalk |
| US7045756B2 (en) | 2004-08-05 | 2006-05-16 | Applera Corporation | Methods and systems for in situ calibration of imaging in biological analysis |
| US20060051796A1 (en) * | 2004-09-09 | 2006-03-09 | Inga Boell | Real time PCR with the addition of pyrophosphatase |
| CN101189505B (zh) * | 2005-04-01 | 2011-11-09 | 3M创新有限公司 | 具有可移除光学模块的多重荧光检测装置 |
| US7507575B2 (en) | 2005-04-01 | 2009-03-24 | 3M Innovative Properties Company | Multiplex fluorescence detection device having removable optical modules |
| US7709249B2 (en) * | 2005-04-01 | 2010-05-04 | 3M Innovative Properties Company | Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector |
| AU2006261346A1 (en) | 2005-06-15 | 2006-12-28 | Gen-Probe Incorporated | Methods for quantitative analysis of a nucleic acid amplification reaction |
| US20060291706A1 (en) | 2005-06-23 | 2006-12-28 | Applera Corporation | Method of extracting intensity data from digitized image |
| US20070009382A1 (en) | 2005-07-05 | 2007-01-11 | William Bedingham | Heating element for a rotating multiplex fluorescence detection device |
| US7754474B2 (en) | 2005-07-05 | 2010-07-13 | 3M Innovative Properties Company | Sample processing device compression systems and methods |
| US7527763B2 (en) | 2005-07-05 | 2009-05-05 | 3M Innovative Properties Company | Valve control system for a rotating multiplex fluorescence detection device |
| US7630849B2 (en) | 2005-09-01 | 2009-12-08 | Applied Biosystems, Llc | Method of automated calibration and diagnosis of laboratory instruments |
| US20070098594A1 (en) | 2005-11-03 | 2007-05-03 | Roche Molecular Systems, Inc. | Analytical multi-spectral optical detection system |
| US7680868B2 (en) | 2005-12-20 | 2010-03-16 | Roche Molecular Systems, Inc. | PCR elbow determination by use of a double sigmoid function curve fit with the Levenburg-Marquardt algorithm and normalization |
| WO2008002765A2 (en) | 2006-06-27 | 2008-01-03 | Applera Corporation | Method and system for compensating for spatial cross-talk |
| WO2008003053A2 (en) * | 2006-06-28 | 2008-01-03 | Applera Corporation | Minimizing effects of dye crosstalk |
| US20080154512A1 (en) * | 2006-07-01 | 2008-06-26 | Harrison Leong | Systems and methods for baselining and real-time pcr data analysis |
| US8005628B2 (en) | 2007-01-29 | 2011-08-23 | Applied Biosystems, Llc | Systems and methods for baseline correction using non-linear normalization |
| ATE518966T1 (de) * | 2007-06-29 | 2011-08-15 | Hoffmann La Roche | Systeme und verfahren zur bestimmung von übersprechungskoeffizienten in pcr und anderen datensets |
| US8339333B2 (en) * | 2008-01-02 | 2012-12-25 | 3M Innovative Properties Company | Methods of reducing perceived image crosstalk in a multiview display |
| AU2009240461A1 (en) | 2008-04-24 | 2009-10-29 | 3M Innovative Properties Company | Analysis of nucleic acid amplification curves using wavelet transformation |
| PT3375891T (pt) * | 2009-09-10 | 2020-05-11 | Diasorin S P A | Métodos e dispositivos para compensação de diafonia espectral em amplificação de ácido nucleico multiplex |
-
2010
- 2010-08-31 PT PT181690405T patent/PT3375891T/pt unknown
- 2010-08-31 PT PT108159138T patent/PT2475788T/pt unknown
- 2010-08-31 ES ES10815913.8T patent/ES2680495T3/es active Active
- 2010-08-31 ES ES18169032T patent/ES2785672T3/es active Active
- 2010-08-31 DK DK18169040.5T patent/DK3375891T3/da active
- 2010-08-31 US US13/394,944 patent/US9938569B2/en active Active
- 2010-08-31 MX MX2012002902A patent/MX352971B/es active IP Right Grant
- 2010-08-31 PT PT181690322T patent/PT3375890T/pt unknown
- 2010-08-31 WO PCT/US2010/047265 patent/WO2011031585A1/en active Application Filing
- 2010-08-31 DK DK10815913.8T patent/DK2475788T3/en active
- 2010-08-31 DK DK18169032.2T patent/DK3375890T3/da active
- 2010-08-31 CN CN201080040425.5A patent/CN102482720B/zh active Active
- 2010-08-31 EP EP10815913.8A patent/EP2475788B1/en active Active
- 2010-08-31 ES ES18169040T patent/ES2791369T3/es active Active
- 2010-08-31 EP EP18169032.2A patent/EP3375890B1/en active Active
- 2010-08-31 EP EP18169040.5A patent/EP3375891B1/en active Active
-
2018
- 2018-03-12 US US15/918,369 patent/US11603559B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20180201987A1 (en) | 2018-07-19 |
| CN102482720B (zh) | 2014-10-08 |
| ES2680495T3 (es) | 2018-09-07 |
| EP3375890A1 (en) | 2018-09-19 |
| MX352971B (es) | 2017-12-15 |
| EP2475788A4 (en) | 2013-05-29 |
| US20120171677A1 (en) | 2012-07-05 |
| EP2475788A1 (en) | 2012-07-18 |
| ES2785672T3 (es) | 2020-10-07 |
| PT2475788T (pt) | 2018-07-24 |
| EP2475788B1 (en) | 2018-04-25 |
| EP3375891B1 (en) | 2020-02-19 |
| PT3375890T (pt) | 2020-04-23 |
| DK3375890T3 (da) | 2020-04-14 |
| DK2475788T3 (en) | 2018-07-30 |
| EP3375891A1 (en) | 2018-09-19 |
| WO2011031585A1 (en) | 2011-03-17 |
| DK3375891T3 (da) | 2020-04-27 |
| ES2791369T3 (es) | 2020-11-04 |
| US11603559B2 (en) | 2023-03-14 |
| CN102482720A (zh) | 2012-05-30 |
| EP3375890B1 (en) | 2020-01-22 |
| PT3375891T (pt) | 2020-05-11 |
| US9938569B2 (en) | 2018-04-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GB | Transfer or rights |
Owner name: DIASORIN S.P.A. |
|
| FG | Grant or registration |