MX2011006577A - Metodos para modular el sexo de aviarios. - Google Patents
Metodos para modular el sexo de aviarios.Info
- Publication number
- MX2011006577A MX2011006577A MX2011006577A MX2011006577A MX2011006577A MX 2011006577 A MX2011006577 A MX 2011006577A MX 2011006577 A MX2011006577 A MX 2011006577A MX 2011006577 A MX2011006577 A MX 2011006577A MX 2011006577 A MX2011006577 A MX 2011006577A
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- nucleic acid
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- egg
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- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
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| JP5554231B2 (ja) * | 2007-06-13 | 2014-07-23 | コモンウェルス サイエンティフィック アンドインダストリアル リサーチ オーガナイゼーション | トリの生産形質の改変 |
| CN108474034A (zh) * | 2015-12-03 | 2018-08-31 | 艾吉伊特有限公司 | 对未孵化卵中的禽类胚胎进行性别确定的方法及其用具 |
| WO2018013759A1 (en) * | 2016-07-13 | 2018-01-18 | Yorktown Technologies, L.P. | Poultry and offspring sex selection |
| CA3065317A1 (en) | 2017-05-31 | 2018-12-06 | Commonwealth Scientific And Industrial Research Organisation | Trait selection in avians |
| CN107236814B (zh) * | 2017-07-14 | 2020-02-28 | 集美大学 | 一种鉴别大黄鱼遗传性别的分子标记及其应用 |
| JP2020536580A (ja) * | 2017-09-19 | 2020-12-17 | ザ ステート オブ イスラエル， ミニストリー オブ アグリカルチャー アンド ルーラル ディヴェロプメント， アグリカルチュラル リサーチ オーガニゼーション （エーアールオー） （ボルカニ センター） | ゲノム編集された鳥 |
| CN110944675B9 (zh) | 2017-12-01 | 2024-08-09 | 苏州瑞博生物技术股份有限公司 | 一种核酸、含有该核酸的组合物与缀合物及制备方法和用途 |
| EP3719126B1 (en) | 2017-12-01 | 2025-01-01 | Suzhou Ribo Life Science Co., Ltd. | Nucleic acid, composition and conjugate containing nucleic acid, preparation method therefor and use thereof |
| DK3719128T3 (da) | 2017-12-01 | 2025-03-03 | Suzhou Ribo Life Science Co Ltd | Dobbelt-strenget oligonukleotid, sammensætning og konjugat bestaende dobbelt-strenget oligonukleotid, fremgangsmåde herfor og anvendelse heraf |
| EP3719127A4 (en) | 2017-12-01 | 2021-10-20 | Suzhou Ribo Life Science Co., Ltd. | NUCLEIC ACID, COMPOSITION AND CONJUGATE WITH IT, MANUFACTURING METHOD AND USE |
| CA3083970A1 (en) | 2017-12-01 | 2019-06-06 | Suzhou Ribo Life Science Co., Ltd. | Nucleic acid, composition and conjugate comprising the same, and preparation method and use thereof |
| AU2018394875B2 (en) | 2017-12-29 | 2023-08-03 | Suzhou Ribo Life Science Co., Ltd. | Conjugates and preparation and use thereof |
| CN108384845A (zh) * | 2018-04-28 | 2018-08-10 | 武汉市农业科学院 | 鸭性别鉴定用rt-pcr引物、试剂盒及鉴定方法 |
| WO2020038377A1 (zh) | 2018-08-21 | 2020-02-27 | 苏州瑞博生物技术有限公司 | 一种核酸、含有该核酸的药物组合物和缀合物及其用途 |
| US11896674B2 (en) | 2018-09-30 | 2024-02-13 | Suzhou Ribo Life Science Co., Ltd. | SiRNA conjugate, preparation method therefor and use thereof |
| WO2020135673A1 (zh) | 2018-12-28 | 2020-07-02 | 苏州瑞博生物技术有限公司 | 一种核酸、含有该核酸的组合物与缀合物及制备方法和用途 |
| KR20210137499A (ko) | 2019-03-05 | 2021-11-17 | 더 스테이트 오브 이스라엘, 미니스트리 오브 애그리컬처 & 루럴 디벨로프먼트, 애그리컬처럴 리서치 오거니제이션, (에이.알.오.), 볼카니 센터 | 게놈-편집 조류 |
| AU2020280439B2 (en) | 2019-05-22 | 2025-08-14 | Suzhou Ribo Life Science Co., Ltd. | Nucleic acid, pharmaceutical composition, conjugate, preparation method, and use |
| KR20220011689A (ko) | 2019-05-22 | 2022-01-28 | 쑤저우 리보 라이프 사이언스 컴퍼니, 리미티드 | 핵산, 약제학적 조성물, 접합체, 제조 방법, 및 용도 |
| US12590304B2 (en) | 2019-05-22 | 2026-03-31 | Suzhou Ribo Life Science Co., Ltd. | Nucleic acid, pharmaceutical composition, conjugate, preparation method, and use |
| CN113795582B (zh) | 2019-05-24 | 2024-05-24 | 苏州瑞博生物技术股份有限公司 | 核酸、药物组合物与缀合物及制备方法和用途 |
| CN110484635B (zh) * | 2019-09-06 | 2020-06-09 | 南京市红山森林动物园管理处 | 一种双垂鹤鸵性别分子鉴定方法及引物 |
Family Cites Families (4)
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|---|---|---|---|---|
| EP1445312B1 (en) * | 2001-11-21 | 2012-12-26 | Astellas Pharma Inc. | Method of inhibiting gene expression |
| AU2003295600A1 (en) * | 2002-11-14 | 2004-06-15 | Dharmacon, Inc. | Functional and hyperfunctional sirna |
| KR100740153B1 (ko) * | 2004-11-24 | 2007-07-16 | (주)아비코아생명공학연구소 | 정소-특이 뉴클레오타이드 서열 |
| JP5554231B2 (ja) * | 2007-06-13 | 2014-07-23 | コモンウェルス サイエンティフィック アンドインダストリアル リサーチ オーガナイゼーション | トリの生産形質の改変 |
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| WO2010068978A1 (en) | 2010-06-24 |
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