MX2010000992A - Vectores y metodos para clonacion de grupos de genes o porciones de los mismos. - Google Patents

Vectores y metodos para clonacion de grupos de genes o porciones de los mismos.

Info

Publication number
MX2010000992A
MX2010000992A MX2010000992A MX2010000992A MX2010000992A MX 2010000992 A MX2010000992 A MX 2010000992A MX 2010000992 A MX2010000992 A MX 2010000992A MX 2010000992 A MX2010000992 A MX 2010000992A MX 2010000992 A MX2010000992 A MX 2010000992A
Authority
MX
Mexico
Prior art keywords
gene clusters
cloning
vectors
portions
methods
Prior art date
Application number
MX2010000992A
Other languages
English (en)
Inventor
Min He
Hongbo Liu
Original Assignee
Wyeth Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth Llc filed Critical Wyeth Llc
Publication of MX2010000992A publication Critical patent/MX2010000992A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un vector lanzadera BAC para facilitar la clonación, transferencia y expresión heteróloga de grupos de genes biosintéticos del metabolito secundario de estreptomicetos; la invención también se refiere a un método de rescate del plásmido utilizando este vector para mejorar el proceso de clonación de grupos de genes biosintéticos para metabolitos secundarios a partir de estreptomicetos sin la generación sofisticada y clasificación de librerías de cósmidos y de BAC; El ADN donado se puede utilizar entonces secuenciación o expresión heteróloga de supuestos grupos de genes metabólicos secundarios.
MX2010000992A 2007-07-27 2008-07-25 Vectores y metodos para clonacion de grupos de genes o porciones de los mismos. MX2010000992A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US96231107P 2007-07-27 2007-07-27
PCT/US2008/009083 WO2009017692A2 (en) 2007-07-27 2008-07-25 Vectors and methods for cloning gene clusters or portions thereof

Publications (1)

Publication Number Publication Date
MX2010000992A true MX2010000992A (es) 2010-03-01

Family

ID=40257042

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010000992A MX2010000992A (es) 2007-07-27 2008-07-25 Vectores y metodos para clonacion de grupos de genes o porciones de los mismos.

Country Status (9)

Country Link
US (1) US20090075283A1 (es)
EP (1) EP2173880A2 (es)
JP (1) JP2010534489A (es)
KR (1) KR20100034055A (es)
AU (1) AU2008282876A1 (es)
BR (1) BRPI0813971A2 (es)
CA (1) CA2694537A1 (es)
MX (1) MX2010000992A (es)
WO (1) WO2009017692A2 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9217154B2 (en) * 2011-12-08 2015-12-22 Bayer Intellectual Property Gmbh Actinomycete integrative and conjugative element from Actinoplanes sp. SE50/110 as plasmid for genetic transformation of related Actinobacteria
EP2677034A1 (en) 2012-06-18 2013-12-25 LEK Pharmaceuticals d.d. Genome sequence based targeted cloning of DNA fragments
WO2020185831A1 (en) * 2019-03-12 2020-09-17 Varigen Biosciences Corporation Expression vector

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935340A (en) * 1985-06-07 1990-06-19 Eli Lilly And Company Method of isolating antibiotic biosynthetic genes
US6495348B1 (en) * 1993-10-07 2002-12-17 Regents Of The University Of Minnesota Mitomycin biosynthetic gene cluster
US6143566A (en) * 1997-06-23 2000-11-07 The Rockfeller University Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof
US20030134398A1 (en) * 2001-09-12 2003-07-17 Sherman David H. Mitomycin biosynthetic gene cluster
JP4532121B2 (ja) * 2002-04-10 2010-08-25 レスポンス バイオメディカル コーポレイション 高感度の免疫クロマトグラフィーアッセイ法
AU2005252673A1 (en) * 2004-06-03 2005-12-22 Wyeth Biosynthetic gene cluster for the production of a complex polyketide

Also Published As

Publication number Publication date
WO2009017692A3 (en) 2009-07-23
JP2010534489A (ja) 2010-11-11
CA2694537A1 (en) 2009-02-05
US20090075283A1 (en) 2009-03-19
KR20100034055A (ko) 2010-03-31
EP2173880A2 (en) 2010-04-14
WO2009017692A2 (en) 2009-02-05
BRPI0813971A2 (pt) 2014-10-07
AU2008282876A1 (en) 2009-02-05

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Legal Events

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