MX2009012733A - Metodo para la deteccion electroquimica de secuencias de acidos nucleicos. - Google Patents

Metodo para la deteccion electroquimica de secuencias de acidos nucleicos.

Info

Publication number
MX2009012733A
MX2009012733A MX2009012733A MX2009012733A MX2009012733A MX 2009012733 A MX2009012733 A MX 2009012733A MX 2009012733 A MX2009012733 A MX 2009012733A MX 2009012733 A MX2009012733 A MX 2009012733A MX 2009012733 A MX2009012733 A MX 2009012733A
Authority
MX
Mexico
Prior art keywords
sequences
nucleic acids
electrochemical detection
detection
hybridation
Prior art date
Application number
MX2009012733A
Other languages
English (en)
Inventor
Abad Encarnacion Lorenzo
Alonso Felix Pariente
Parra Monica Revenga
Mendiola Tania Garcia
Original Assignee
Univ Madrid Autonoma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Madrid Autonoma filed Critical Univ Madrid Autonoma
Publication of MX2009012733A publication Critical patent/MX2009012733A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/89—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members with hetero atoms directly attached to the ring nitrogen atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/90—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having more than three double bonds between ring members or between ring members and non-ring members
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pyridine Compounds (AREA)

Abstract

Es objeto de la presente invención un nuevo compuesto derivado de rutenio, de fórmula (I), denominado complejo de pentamin rutenio [3-(2-fenantren-9-il-vinil)piridina], así como su empleo como indicador electroquímico de hibridación en un método para la detección de hibridación entre secuencias de ácidos nucleicos, para la cuantificación de dichas secuencias, así como la detección de la presencia de una base desapareada y su posición dentro de una secuencia de ácido nucleico.
MX2009012733A 2007-05-25 2008-05-23 Metodo para la deteccion electroquimica de secuencias de acidos nucleicos. MX2009012733A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES200701446A ES2310125B1 (es) 2007-05-25 2007-05-25 Metodo para la deteccion electroquimica de secuencias de acidos nucleicos.
PCT/ES2008/000364 WO2008145785A1 (es) 2007-05-25 2008-05-23 Método para la detección electroquímica de secuencias de ácidos nucléicos

Publications (1)

Publication Number Publication Date
MX2009012733A true MX2009012733A (es) 2009-12-11

Family

ID=40074601

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009012733A MX2009012733A (es) 2007-05-25 2008-05-23 Metodo para la deteccion electroquimica de secuencias de acidos nucleicos.

Country Status (11)

Country Link
US (1) US20100236942A1 (es)
EP (1) EP2168949A4 (es)
JP (1) JP2010529007A (es)
CN (1) CN101796029B (es)
AU (1) AU2008257342A1 (es)
BR (1) BRPI0812108A2 (es)
CA (1) CA2687933A1 (es)
ES (1) ES2310125B1 (es)
MX (1) MX2009012733A (es)
RU (1) RU2470938C2 (es)
WO (1) WO2008145785A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU182822U1 (ru) * 2016-12-30 2018-09-04 Федеральное государственное бюджетное учреждение науки Институт химии и химической технологии Сибирского отделения Российской академии наук - обособленное подразделение ФИЦ КНЦ СО РАН Мультиплексный электрохимический биочип для выявления опухолеассоциированных белков-биомаркеров рака легкого
RU179185U1 (ru) * 2016-12-30 2018-05-03 Федеральное государственное бюджетное учреждение науки "Институт химии и химических технологий" Сибирского Отделения Российской академии наук - обособленное подразделение ФИЦ КНЦ СО РАН Мультиплексная система для диагностики онкологических заболеваний
WO2020129452A1 (ja) * 2018-12-18 2020-06-25 株式会社ヨコオ 計測装置及び計測方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9206671D0 (en) 1992-03-27 1992-05-13 Cranfield Biotech Ltd Electrochemical detection of dna hybridisation
RU2243231C2 (ru) * 1997-09-12 2004-12-27 Эксикон А/С Бициклические аналоги нуклеозидов, нуклеотидов и олигонуклеотидов
JP2003144152A (ja) * 2001-11-09 2003-05-20 Fuji Photo Film Co Ltd ターゲット核酸断片の検出方法及びターゲット核酸断片の検出キット
GB0205455D0 (en) * 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7258978B2 (en) * 2002-11-06 2007-08-21 Geneohm Sciences Electrochemical method to measure DNA attachment to an electrode surface in the presence of molecular oxygen
ATE481502T1 (de) 2003-05-02 2010-10-15 Geneohm Sciences Elektrochemisches verfahren zur messung der bindung von dna an eine elektrodenoberfläche in gegenwart von molekularem sauerstoff
US7741033B2 (en) * 2003-05-13 2010-06-22 Trustees Of Boston College Electrocatalytic nucleic acid hybridization detection

Also Published As

Publication number Publication date
RU2009148277A (ru) 2011-06-27
BRPI0812108A2 (pt) 2014-11-25
CN101796029A (zh) 2010-08-04
WO2008145785A1 (es) 2008-12-04
US20100236942A1 (en) 2010-09-23
JP2010529007A (ja) 2010-08-26
ES2310125A1 (es) 2008-12-16
CN101796029B (zh) 2012-05-23
CA2687933A1 (en) 2008-12-04
RU2470938C2 (ru) 2012-12-27
EP2168949A1 (en) 2010-03-31
AU2008257342A1 (en) 2008-12-04
ES2310125B1 (es) 2010-01-07
EP2168949A4 (en) 2011-05-25

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