MX165881B - Produccion de alto nivel de la hormona del crecimiento de los cerdos - Google Patents
Produccion de alto nivel de la hormona del crecimiento de los cerdosInfo
- Publication number
- MX165881B MX165881B MX026442A MX2644286A MX165881B MX 165881 B MX165881 B MX 165881B MX 026442 A MX026442 A MX 026442A MX 2644286 A MX2644286 A MX 2644286A MX 165881 B MX165881 B MX 165881B
- Authority
- MX
- Mexico
- Prior art keywords
- protein
- temperature
- growth hormone
- medium
- maintained
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 101000868144 Sus scrofa Somatotropin Proteins 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 6
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 102000018997 Growth Hormone Human genes 0.000 abstract 3
- 108010051696 Growth Hormone Proteins 0.000 abstract 3
- 241000282898 Sus scrofa Species 0.000 abstract 3
- 239000000122 growth hormone Substances 0.000 abstract 3
- 230000003054 hormonal effect Effects 0.000 abstract 3
- 241000588724 Escherichia coli Species 0.000 abstract 2
- 102000009661 Repressor Proteins Human genes 0.000 abstract 2
- 108010034634 Repressor Proteins Proteins 0.000 abstract 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 2
- 238000000855 fermentation Methods 0.000 abstract 2
- 230000004151 fermentation Effects 0.000 abstract 2
- 229910052760 oxygen Inorganic materials 0.000 abstract 2
- 239000001301 oxygen Substances 0.000 abstract 2
- 241000701959 Escherichia virus Lambda Species 0.000 abstract 1
- 230000010261 cell growth Effects 0.000 abstract 1
- 230000012010 growth Effects 0.000 abstract 1
- 230000006698 induction Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/813—Continuous fermentation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/818—Aeration or oxygen transfer technique
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Endocrinology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Heat Treatment Of Steel (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
La presente invención se refiere a un método para producir la hormona del crecimiento de cerdos, caracterizada porque comprende: inocular un medio de fermentacion acuoso con un cultivo de una cepa E. colo transformante que contiene un vector de expresión que dirige la expresión de la hormona del crecimiento de cerdos bajo el control de un promotor-operador de fago lambda, y un vector de expresión que dirige la expresión de la proteína represora, sensible a la temperatura cI857, desarrollar la cepa transformante en el medio de fermenetación durante un periódo de desarrollo incial durante el cual se mantiene el nivel de oxígeno disuelto en el medio de 20 a 60 por ciento de saturación, y se mantiene la temperatura del medio de fermentación cuando menos aproximadamente a 42 grados C para inactivar la proteína represora, sensible a la temperatura; iniciar de esa manera un periódo de inducción durante el cual se produce la hormono de crecimiento de cerco; reducir la temperatura de aproximadamente 39-41 grados C, y cultivar la cepa transformante durante el resto del periódo de inducción durante el cual se mantiene el nivel de oxígeno disuelto en el medio de 10 - 40 por ciento de saturación; y recuperar la hormona de crecimiento de cerdos de las células transformantes.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/751,976 US4788144A (en) | 1985-06-28 | 1985-06-28 | Fermentation process for the high level production of swine growth |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX165881B true MX165881B (es) | 1992-12-08 |
Family
ID=25024318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX026442A MX165881B (es) | 1985-06-28 | 1986-06-27 | Produccion de alto nivel de la hormona del crecimiento de los cerdos |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US4788144A (es) |
| EP (1) | EP0208489B1 (es) |
| AT (1) | ATE78512T1 (es) |
| BR (1) | BR8602996A (es) |
| CA (1) | CA1262533A (es) |
| DE (1) | DE3686102T2 (es) |
| DK (1) | DK303586A (es) |
| ES (1) | ES2025383A6 (es) |
| IE (1) | IE60142B1 (es) |
| IL (1) | IL79271A0 (es) |
| MX (1) | MX165881B (es) |
| PT (1) | PT82860B (es) |
| ZA (1) | ZA864800B (es) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4705848A (en) * | 1986-06-02 | 1987-11-10 | International Minerals & Chemical Corp. | Isolation of bioactive, monomeric growth hormone |
| US5541086A (en) * | 1986-12-31 | 1996-07-30 | Lucky, Ltd. | Method for the production of porcine growth hormone using a synthetic gene in yeast cells |
| KR920003663B1 (ko) * | 1986-12-31 | 1992-05-06 | 주식회사 럭키 | 합성유전자에 의한 효모세포로부터 돼지 성장 호르몬의 제조방법 |
| GB8701848D0 (en) * | 1987-01-28 | 1987-03-04 | Celltech Ltd | Polypeptides |
| US5104796A (en) * | 1987-04-06 | 1992-04-14 | International Minerals & Chemical Corp. | High titer production of human somatomedin c |
| US5637566A (en) * | 1988-08-24 | 1997-06-10 | Southern Cross Biotech Pty. Ltd. | Method of improving carcass quality by administering growth hormone |
| US4897349A (en) * | 1989-04-28 | 1990-01-30 | Medchem Products, Inc. | Biosynthesis of hyaluronic acid |
| EP0723594A1 (en) * | 1993-10-15 | 1996-07-31 | Mallinckrodt Veterinary, Inc. | A plasmid vector useful for the expression of a foreign dna sequence |
| KR100725336B1 (ko) | 2005-12-26 | 2007-06-07 | 씨제이 주식회사 | 코리네형 세균에서 작동하는 신규 유도발현 벡터 시스템 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4167450A (en) * | 1977-07-13 | 1979-09-11 | University Of New Hampshire | Method and apparatus for the production of secondary metabolites by the maintenance-state cultivation of microorganisms |
| DE3015699C2 (de) * | 1979-04-26 | 1982-07-15 | Asahi Kasei Kogyo K.K., Osaka | Herstellung eines Plasminogen-Aktivators |
| DE3279270D1 (en) * | 1981-09-02 | 1989-01-12 | Biogen Nv | Amplified expression of dna sequences |
| EP0103395A3 (en) * | 1982-08-17 | 1985-05-22 | Biogen N.V. | Dna sequences, recombinant dna molecules and processes for producing bovine growth hormone-like polypeptides in high yield |
| CA1341012C (en) * | 1982-09-27 | 2000-06-06 | Biogen, Inc. | Dna sequences, recombinant dna molecules and processes for producing swine growth hormone-like polypeptides |
| US4512922A (en) * | 1982-12-22 | 1985-04-23 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
| US4518526A (en) * | 1982-12-22 | 1985-05-21 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
| US4511502A (en) * | 1982-12-22 | 1985-04-16 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
| BG49718A3 (en) * | 1983-07-15 | 1992-01-15 | Bio- Technology General Corp | Method for preparing of polypeptid with superoxiddismutasne activitty |
| US4569790A (en) * | 1984-03-28 | 1986-02-11 | Cetus Corporation | Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions |
| US4762784A (en) * | 1985-07-15 | 1988-08-09 | International Minerals & Chemical Corp. | Fermemtation process for the high level production of bovine growth hormone |
-
1985
- 1985-06-28 US US06/751,976 patent/US4788144A/en not_active Expired - Lifetime
-
1986
- 1986-06-26 DK DK303586A patent/DK303586A/da not_active Application Discontinuation
- 1986-06-27 MX MX026442A patent/MX165881B/es unknown
- 1986-06-27 IL IL79271A patent/IL79271A0/xx not_active IP Right Cessation
- 1986-06-27 EP EP86305052A patent/EP0208489B1/en not_active Expired - Lifetime
- 1986-06-27 ZA ZA864800A patent/ZA864800B/xx unknown
- 1986-06-27 CA CA000512728A patent/CA1262533A/en not_active Expired
- 1986-06-27 IE IE173086A patent/IE60142B1/en not_active IP Right Cessation
- 1986-06-27 PT PT82860A patent/PT82860B/pt unknown
- 1986-06-27 BR BR8602996A patent/BR8602996A/pt unknown
- 1986-06-27 AT AT86305052T patent/ATE78512T1/de not_active IP Right Cessation
- 1986-06-27 ES ES8600037A patent/ES2025383A6/es not_active Expired - Fee Related
- 1986-06-27 DE DE8686305052T patent/DE3686102T2/de not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DK303586A (da) | 1986-12-29 |
| US4788144A (en) | 1988-11-29 |
| DK303586D0 (da) | 1986-06-26 |
| DE3686102T2 (de) | 1993-01-07 |
| IL79271A0 (en) | 1986-09-30 |
| PT82860B (pt) | 1988-04-21 |
| PT82860A (en) | 1986-07-01 |
| EP0208489A2 (en) | 1987-01-14 |
| CA1262533C (en) | 1989-10-31 |
| CA1262533A (en) | 1989-10-31 |
| EP0208489B1 (en) | 1992-07-22 |
| BR8602996A (pt) | 1987-02-17 |
| ES2025383A6 (es) | 1992-03-16 |
| DE3686102D1 (de) | 1992-08-27 |
| IE60142B1 (en) | 1994-06-01 |
| EP0208489A3 (en) | 1988-07-13 |
| ATE78512T1 (de) | 1992-08-15 |
| IE861730L (en) | 1986-12-28 |
| ZA864800B (en) | 1987-02-25 |
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