MD56Y - Method for selecting salinization-resistant tomato genotypes - Google Patents
Method for selecting salinization-resistant tomato genotypesInfo
- Publication number
- MD56Y MD56Y MDS20090044A MDS20090044A MD56Y MD 56 Y MD56 Y MD 56Y MD S20090044 A MDS20090044 A MD S20090044A MD S20090044 A MDS20090044 A MD S20090044A MD 56 Y MD56 Y MD 56Y
- Authority
- MD
- Moldova
- Prior art keywords
- pollen
- indices
- resistance
- salinization
- expressed
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 title abstract 2
- 240000003768 Solanum lycopersicum Species 0.000 title abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 2
- 229930006000 Sucrose Natural products 0.000 abstract 2
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 abstract 2
- 239000004327 boric acid Substances 0.000 abstract 2
- 239000012153 distilled water Substances 0.000 abstract 2
- 235000015097 nutrients Nutrition 0.000 abstract 2
- 239000005720 sucrose Substances 0.000 abstract 2
- 230000035899 viability Effects 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 230000035784 germination Effects 0.000 abstract 1
- 230000010152 pollination Effects 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Inventia se refera la agricultura, in particular la metodele de selectare a genotipurilor de tomate rezistente la salinizare. Metoda, conform inventiei, include colectarea florilor complet deschise de fiecare genotip, separarea polenului din antere, cultivarea la temperatura de 27°?timp de 3 ore, a unei parti de polen martor pe un mediu nutritiv, continand, in g/L:zaharoza 145...155 acid boric 0,5?0,7 apa distilata restul,si a altei parti de polen de proba pe un mediu nutritiv, continand, in g/L:zaharoza 145...155 acid boric 0,5...0,7clorura de natriu 0,245...0,255apa distilata restul,determinarea viabilitatii polenului la martor si la proba prin raportul dintre numarul de grauncioare de polen germinate si numarul total de grauncioare analizate, exprimate in procente; determinarea lungimii tuburilor polinice cu ajutorul ocular-micrometrului; calcularea indicilor de rezistenta a polenuluiprin raportul viabilitatii polenului la proba catre martor, exprimati in procente; si a indicilor de rezistenta a tuburilor polinice prin raportul lungimii tuburilor polinice la proba catre martor, exprimati in procente; si calcularea indicilor de rezistenta medie integrala a genotipurilor la salinizare, exprimati prin semisuma indicilor de rezistenta a polenului si a tuburilor polinice, totodata se selecteaza genotipurile, la care indicii de rezistenta la salinizare sunt mai mari.The invention relates to agriculture, in particular to the methods of selection of tomato genotypes resistant to salinization. The method, according to the invention, includes the collection of flowers completely opened by each genotype, separation of pollen from anthers, cultivation at 27 ° C for 3 hours, part of control pollen on a nutrient medium, containing, in g / L: sucrose 145 ... 155 boric acid 0.5? 0.7 distilled water, and other pollen portion on a nutrient medium, containing, in g / L: sucrose 145 ... 155 boric acid 0.5. 0.7 sodium chloride 0.245 ... 0.255 distilled water, determination of pollen viability in the control and sample by the ratio between the number of germination pollen germinated and the total number of analyzed pollination, expressed as a percentage; determining the length of the pollen tubes using the ocular-micrometer; calculation of pollen resistance indices based on the pollen viability ratio in the sample to the control, expressed in percentages; and of the indices of the resistance of the pollen tubes by the ratio of the length of the pollen tubes to the sample to the witness, expressed in percentages; and the calculation of the indices of the integral integral resistance of the genotypes to salinization, expressed by the half-sum of the indices of resistance of the pollen and of the pollen tubes, at the same time the genotypes are selected, at which the indices of resistance to the salinization are higher.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20090044A MD56Z (en) | 2009-03-31 | 2009-03-31 | Method for selecting salinization-resistant tomato genotypes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20090044A MD56Z (en) | 2009-03-31 | 2009-03-31 | Method for selecting salinization-resistant tomato genotypes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD56Y true MD56Y (en) | 2009-08-31 |
| MD56Z MD56Z (en) | 2010-03-31 |
Family
ID=41120154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20090044A MD56Z (en) | 2009-03-31 | 2009-03-31 | Method for selecting salinization-resistant tomato genotypes |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD56Z (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD653Z (en) * | 2013-02-28 | 2014-02-28 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Method for determination of drought-resistant genotypes of tomato |
-
2009
- 2009-03-31 MD MDS20090044A patent/MD56Z/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD56Z (en) | 2010-03-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| KA4Y | Short-term patent lapsed due to non-payment of fees (with right of restoration) |