MD56Y - Method for selecting salinization-resistant tomato genotypes - Google Patents
Method for selecting salinization-resistant tomato genotypesInfo
- Publication number
- MD56Y MD56Y MDS20090044A MDS20090044A MD56Y MD 56 Y MD56 Y MD 56Y MD S20090044 A MDS20090044 A MD S20090044A MD S20090044 A MDS20090044 A MD S20090044A MD 56 Y MD56 Y MD 56Y
- Authority
- MD
- Moldova
- Prior art keywords
- pollen
- resistance
- salinization
- sample
- genotypes
- Prior art date
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Inventia se refera la agricultura, in particular la metodele de selectare a genotipurilor de tomate rezistente la salinizare. Metoda, conform inventiei, include colectarea florilor complet deschise de fiecare genotip, separarea polenului din antere, cultivarea la temperatura de 27°?timp de 3 ore, a unei parti de polen martor pe un mediu nutritiv, continand, in g/L:zaharoza 145...155 acid boric 0,5?0,7 apa distilata restul,si a altei parti de polen de proba pe un mediu nutritiv, continand, in g/L:zaharoza 145...155 acid boric 0,5...0,7clorura de natriu 0,245...0,255apa distilata restul,determinarea viabilitatii polenului la martor si la proba prin raportul dintre numarul de grauncioare de polen germinate si numarul total de grauncioare analizate, exprimate in procente; determinarea lungimii tuburilor polinice cu ajutorul ocular-micrometrului; calcularea indicilor de rezistenta a polenuluiprin raportul viabilitatii polenului la proba catre martor, exprimati in procente; si a indicilor de rezistenta a tuburilor polinice prin raportul lungimii tuburilor polinice la proba catre martor, exprimati in procente; si calcularea indicilor de rezistenta medie integrala a genotipurilor la salinizare, exprimati prin semisuma indicilor de rezistenta a polenului si a tuburilor polinice, totodata se selecteaza genotipurile, la care indicii de rezistenta la salinizare sunt mai mari.The invention relates to agriculture, in particular to the methods of selection of tomato genotypes resistant to salinization. The method, according to the invention, includes the collection of flowers completely opened by each genotype, separation of pollen from anthers, cultivation at 27 ° C for 3 hours, part of control pollen on a nutrient medium, containing, in g / L: sucrose 145 ... 155 boric acid 0.5? 0.7 distilled water, and other pollen portion on a nutrient medium, containing, in g / L: sucrose 145 ... 155 boric acid 0.5. 0.7 sodium chloride 0.245 ... 0.255 distilled water, determination of pollen viability in the control and sample by the ratio between the number of germination pollen germinated and the total number of analyzed pollination, expressed as a percentage; determining the length of the pollen tubes using the ocular-micrometer; calculation of pollen resistance indices based on the pollen viability ratio in the sample to the control, expressed in percentages; and of the indices of the resistance of the pollen tubes by the ratio of the length of the pollen tubes to the sample to the witness, expressed in percentages; and the calculation of the indices of the integral integral resistance of the genotypes to salinization, expressed by the half-sum of the indices of resistance of the pollen and of the pollen tubes, at the same time the genotypes are selected, at which the indices of resistance to the salinization are higher.
Description
[0001] Invenţia se referă la agricultură, în particular la metodele de selectare a genotipurilor de tomate rezistente la salinizare.[0001] The invention relates to agriculture, in particular to methods for selecting tomato genotypes resistant to salinization.
[0002] Este cunoscută metoda de estimare a rezistenţei tomatelor la salinizare după germinarea seminţelor pe soluţii saline, care constă în determinarea germinării seminţelor pe fondul de salinizare şi care poate fi recomandată pentru stabilirea rezistenţei materialului ameliorativ. Această metodă include cultivarea seminţelor pe soluţie cu clorură de natriu cu concentraţia 0,92% pe fondul termic de 22°C timp de 10 zile şi numărarea zilnică a seminţelor germinate. Rezistenţa mostrelor se stabileşte prin raportul numărului de seminţe germinate în varianta martor (pe apă distilată) faţă de acest indiciu în experienţă (pe soluţie cu clorură de natriu) [1]. Însă această metodă este destul de îndelungată şi anevoioasă. În afară de aceasta, ea nu permite de a stabili în mod deplin rezistenţa genotipurilor, deoarece nu caracterizează reacţia lor la etapa reproductivă, deşi anume la această perioadă plantele sunt foarte sensibile la acţiunea salinizării. În consecinţă, evaluarea rezistenţei polenului pe mediul, care modelează influenţa salinizării, permite în mod rapid şi deplin de a estima rezistenţa şi pe această bază a selecta genotipurile valoroase.[0002] There is a known method for estimating the resistance of tomatoes to salinization after seed germination in saline solutions, which consists in determining the germination of seeds in a salinization environment and which can be recommended for establishing the resistance of the ameliorating material. This method includes cultivating seeds in a solution of sodium chloride with a concentration of 0.92% at a temperature of 22°C for 10 days and counting the germinated seeds daily. The resistance of samples is determined by the ratio of the number of germinated seeds in the control variant (on distilled water) to this indicator in the experiment (on a solution of sodium chloride) [1]. However, this method is quite time-consuming and laborious. In addition, it does not allow to fully establish the resistance of genotypes, since it does not characterize their reaction at the reproductive stage, although it is during this period that plants are very sensitive to the action of salinization. Consequently, the evaluation of pollen resistance to the environment, which models the influence of salinization, allows for a rapid and complete estimation of resistance and, on this basis, to select valuable genotypes.
[0003] Problema pe care o rezolvă invenţia constă în creşterea nivelului de autenticitate a estimării rezistenţei genotipurilor la salinizare pe baza utilizării unui indiciu de rezistenţă medie integrală.[0003] The problem solved by the invention consists in increasing the level of authenticity of the estimation of genotypes' resistance to salinization based on the use of an integral average resistance index.
[0004] Esenţa invenţiei constă în aceea că metoda de selectare a genotipurilor de tomate rezistente la salinizare, conform invenţiei, include colectarea florilor complet deschise de fiecare genotip, separarea polenului din antere, cultivarea la temperatura de 27°C timp de 3 ore, a unei părţi de polen martor pe un mediu nutritiv, conţinând, în g/L:[0004] The essence of the invention lies in the fact that the method for selecting tomato genotypes resistant to salinization, according to the invention, includes collecting fully opened flowers of each genotype, separating pollen from the anthers, cultivating at a temperature of 27°C for 3 hours, a portion of control pollen on a nutrient medium, containing, in g/L:
zaharozăsucrose
145...155145...155
acid boricboric acid
0,5…0,70.5…0.7
apă distilatădistilled water
restul,the rest,
[0006] şi a altei părţi de polen de probă pe un mediu nutritiv, conţinând, în g/L:[0006] and another portion of sample pollen on a nutrient medium, containing, in g/L:
zaharozăsucrose
145...155145...155
acid boricboric acid
0,5...0,70.5...0.7
clorură de natriusodium chloride
0,245...0,2550.245...0.255
apă distilatădistilled water
restul,the rest,
[0008] determinarea viabilităţii polenului la martor şi la probă prin raportul dintre numărul de grăuncioare de polen germinate şi numărul total de grăuncioare analizate, exprimate în procente; determinarea lungimii tuburilor polinice cu ajutorul ocular-micrometrului; calcularea indicilor de rezistenţă a polenului prin raportul viabilităţii polenului la probă către martor, exprimaţi în procente; şi a indicilor de rezistenţă a tuburilor polinice prin raportul lungimii tuburilor polinice la probă către martor, exprimaţi în procente; şi calcularea indicilor de rezistenţă medie integrală a genotipurilor la salinizare, exprimaţi prin semisuma indicilor de rezistenţă a polenului şi a tuburilor polinice, totodată se selectează genotipurile, la care indicii de rezistenţă la salinizare sunt mai mari.[0008] determining the pollen viability in the control and in the sample by the ratio between the number of germinated pollen grains and the total number of grains analyzed, expressed in percentages; determining the length of the pollen tubes using the eyepiece-micrometer; calculating the pollen resistance indices by the ratio of pollen viability in the sample to the control, expressed in percentages; and the pollen tube resistance indices by the ratio of pollen tube length in the sample to the control, expressed in percentages; and calculating the average integral resistance indices of the genotypes to salinization, expressed by the half-sum of the pollen and pollen tube resistance indices, at the same time selecting the genotypes, in which the salinization resistance indices are higher.
[0009] Rezultatul constă în posibilitatea estimării rezistenţei la salinizare a genotipurilor de tomate la etapa de polen matur, ce permite diferenţierea genotipurilor pe baza unui indiciu de rezistenţă medie integrală, care îmbină rezistenţa polenului şi tuburilor polinice, ce la final îmbunătăţeşte calitatea şi autenticitatea evaluării şi permite includerea genotipurilor valoroase în procesul de hibridare şi selecţie în acelaşi an, sporind astfel procesul ameliorativ.[0009] The result consists in the possibility of estimating the salinity resistance of tomato genotypes at the mature pollen stage, which allows the differentiation of genotypes based on an index of integral average resistance, which combines the resistance of pollen and pollen tubes, which ultimately improves the quality and authenticity of the evaluation and allows the inclusion of valuable genotypes in the hybridization and selection process in the same year, thus enhancing the amelioration process.
[0010] Exemplu de realizare a invenţiei[0010] Embodiment of the invention
[0011] În scopul estimării mostrelor de tomate după rezistenţa la salinizare au fost utilizate 8 genotipuri: soiul Ceros - 70; speciile spontane: Lycopersicon hirsutum gl., Lycopersicon cheesmanii, Lycopersicon chilenzi, Lycopersicon peruvianum; varietăţile semiculturale: Lycopersicon esculentum var. succenturiatum, Lycopersicon esculentum var. pruniforme, Lycopersicon esculentum var. recemigerum. Plantele au fost cultivate în condiţii de câmp până la înflorire. La fiecare genotip de pe 15…20 de plante au fost colectate flori complet deschise, apoi au fost separate anterele, care se amplasează în pachete de hârtie pergament pentru uscarea uşoară în condiţii de temperatură 25…26°C timp de 6 ore. După expirarea termenului dat polenul a fost izolat din antere prin scuturarea mecanică şi semănat pe două variante de medii nutritive: martor şi probă, care modelează condiţii de salinizare.[0011] In order to estimate the tomato samples for resistance to salinity, 8 genotypes were used: the Ceros - 70 variety; spontaneous species: Lycopersicon hirsutum gl., Lycopersicon cheesmanii, Lycopersicon chilenzi, Lycopersicon peruvianum; semi-cultivated varieties: Lycopersicon esculentum var. succenturiatum, Lycopersicon esculentum var. pruniforme, Lycopersicon esculentum var. recemigerum. The plants were grown in field conditions until flowering. For each genotype, fully opened flowers were collected from 15…20 plants, then the anthers were separated, which were placed in parchment paper packages for easy drying at a temperature of 25…26°C for 6 hours. After the expiration of the given period, the pollen was isolated from the anthers by mechanical shaking and sown on two variants of nutrient media: control and sample, which model salinization conditions.
[0012] Mediile nutritive pentru cultivarea polenului au conţinut următoarele ingrediente, g/L:[0012] The nutrient media for pollen cultivation contained the following ingredients, g/L:
Mediul 1 zaharozăMedium 1 sucrose
145145
Martor acid boricBoric acid control
0,50.5
apă distilatădistilled water
restul (până la 1000 mL).the rest (up to 1000 mL).
Mediul 2Environment 2
zaharoză 145sucrose 145
ProbăSample
acid boric 0,5boric acid 0.5
clorură de natriusodium chloride
0,2450.245
apă distilatădistilled water
restul (până la 1000 mL).the rest (up to 1000 mL).
[0015] Polenul din ambele variante (martor şi probă) a fost semănat într-o picătură de mediu nutritiv pe sticle portobiect, care au fost amplasate în cupe Petri pe strat de hârtie de filtru umed, apoi au fost transferate în termostat cu regim termic de 27°C cu durata de 3 ore. În fiecare variantă au fost analizate nu mai puţin de 600 grăuncioare de polen. Analiza preparatelor s-a efectuat la microscop, fiind determinaţi următorii indici:[0015] The pollen from both variants (control and sample) was sown in a drop of nutrient medium on glass slides, which were placed in Petri dishes on a layer of wet filter paper, then transferred to a thermostat with a thermal regime of 27°C for 3 hours. In each variant, no less than 600 pollen grains were analyzed. The analysis of the preparations was carried out under a microscope, the following indices being determined:
[0016] 1) viabilitatea polenului prin raportul dintre numărul grăuncioarelor de polen germinate şi numărul grăuncioarelor de polen analizate;[0016] 1) pollen viability by the ratio between the number of germinated pollen grains and the number of pollen grains analyzed;
[0017] 2) lungimea tuburilor polinice (TP) în diviziuni ale ocular-micrometrului.[0017] 2) pollen tube length (TP) in eyepiece-micrometer divisions.
[0018] Pentru estimarea rezistenţei genotipurilor au fost utilizaţi 2 indici: rezistenţa polenului (R) şi rezistenţa tuburilor polinice (R1), care s-au calculat după formulele:[0018] To estimate the resistance of genotypes, 2 indices were used: pollen resistance (R) and pollen tube resistance (R1), which were calculated according to the formulas:
[0019] [0019]
, \tabunde :, \tabhere :
[0020] R - rezistenţa polenului;[0020] R - pollen resistance;
[0021] P - viabilitatea polenului în varianta probă;[0021] P - pollen viability in the sample variant;
[0022] M - viabilitatea polenului în varianta martor.[0022] M - pollen viability in the control variant.
[0023] [0023]
, \tabunde, \tabunde
[0024] R1 - rezistenţa tuburilor polinice;[0024] R1 - pollen tube resistance;
[0025] P1 - lungimea tuburilor polinice în varianta probă;[0025] P1 - length of pollen tubes in the sample variant;
[0026] M1 - lungimea tuburilor polinice în varianta martor.[0026] M1 - length of pollen tubes in the control variant.
[0027] În scopul calculării rezistenţei medii integrale (R2) sumăm valorile stabilite R şi R1, iar suma o divizăm la doi. Indicele stabilit R2 permite de a estima şi diferenţia genotipurile în mod mai exact şi deplin (tabelul).[0027] In order to calculate the mean integral resistance (R2), we sum the established values R and R1, and divide the sum by two. The established index R2 allows us to estimate and differentiate genotypes more accurately and fully (table).
[0028] Tabel[0028] Table
Genotipulgenotype
VariantaVariant
Viabilitatea polenului, %Pollen viability, %
Rezistenţa polenului, %, RPollen resistance, %, R
Lungimea TP (diviz.oc.-micr.)TP length (oc.-micr. division)
Rezistenţa TP, %, R1TP resistance, %, R1
Rezistenţa medie integrală, %, R2Average integral resistance, %, R2
Soiul Ceros-70Variety Ceros-70
martorBLANK
36,1±1,8336.1±1.83
9,7±0,609.7±0.60
probăsample
22,4±1,7622.4±1.76
62,0±1,8062.0±1.80
2,7±0,672.7±0.67
27,8±0,6727.8±0.67
44,7±1,24*44.7±1.24*
Lycopersicon hirsutum gl.Lycopersicon hirsutum gl.
martorBLANK
49,1±1,7049.1±1.70
3,6±0,223.6±0.22
probăsample
22,8±1,4522.8±1.45
46,4±1,5746.4±1.57
1,5±0,151.5±0.15
41,7±0,1941.7±0.19
44,3±0,88*44.3±0.88*
Lycopersicon cheesmaniiLycopersicon cheesmanii
martorBLANK
83,9±1,6083.9±1.60
6,3±0,226.3±0.22
probăsample
42,8±1,3442.8±1.34
51,0±1,3251.0±1.32
2,0±0,172.0±0.17
31,7±0,2031.7±0.20
41,3±0,76*41.3±0.76*
Lycopersicon chilenziLycopersicon chilenzi
martorBLANK
65,4±1,9465.4±1.94
9,7±0,389.7±0.38
probăsample
27,0±1,7827.0±1.78
41,3±1,8641.3±1.86
3,7±0,513.7±0.51
38,1±0,5538.1±0.55
39,7±1,20*39.7±1.20*
Lycopersicon esculentum var. succenturiatumLycopersicon esculentum var. succenturiatum
martorBLANK
55,8±1,4855.8±1.48
7,9±0,457.9±0.45
probăsample
18,4±1,4518.4±1.45
33,0±1,4733.0±1.47
3,2±0,273.2±0.27
40,5±0,3640.5±0.36
36,7±0,81**36.7±0.81**
Lycopersicon peruvianumLycopersicon peruvianum
martorBLANK
34,8±1,6834.8±1.68
8,6±0,258.6±0.25
probăsample
10,7±1,5510.7±1.55
30,7±1,6230.7±1.62
3,4±0,403.4±0.40
39,5±0,3339.5±0.33
35,1±0,98**35.1±0.98**
Lycopersicon esculentum var. pruniformeLycopersicon esculentum var. uniform
martorBLANK
63,4±1,8863.4±1.88
6,3±0,416.3±0.41
probăsample
20,2±1,3720.2±1.37
31,9±1,6331.9±1.63
1,5±0,171.5±0.17
23,8±0,2623.8±0.26
27,8±0,95***27.8±0.95***
Lycopersicon esculentum var. racemigerumLycopersicon esculentum var. racemigerum
martorBLANK
66,4±1,5566.4±1.55
7,5±0,197.5±0.19
probăsample
18,3±1,5018.3±1.50
27,6±1,5327.6±1.53
1,5±0,241.5±0.24
20,0±0,2220.0±0.22
23,8±0,88***23.8±0.88***
[0030] * - deosebirile între genotipurile date şi celelalte sunt autentice la nivel de P<0,05[0030] * - differences between given genotypes and the others are authentic at the P<0.05 level
[0031] ** - deosebirile între genotipurile date şi celelalte sunt autentice la nivel de P<0,05[0031] ** - differences between given genotypes and the others are authentic at the P<0.05 level
[0032] *** - deosebirile între genotipurile date şi celelalte sunt autentice la nivel de P<0,01[0032] *** - the differences between the given genotypes and the others are authentic at the P<0.01 level
[0033] Rezultatele obţinute au demonstrat că, conform invenţiei, cultivarea polenului pe mediul selectiv cu clorură de natriu şi prelucrarea statistică a valorilor de rezistenţă a polenului şi tuburilor polinice permit diferenţierea autentică a genotipurilor de tomate după rezistenţa la salinizare pe baza rezistenţei medii integrale. Conform datelor obţinute, rezistenţă maximală la acţiunea salinizării au demonstrat soiul Ceros-70, speciile spontane: Lycopersicon hirsutum gl., Lycopersicon cheesmanii, Lycopersicon chilenzi; apoi s-au evidenţiat 2 genotipuri cu un nivel de rezistenţă mai mic: Lycopersicon esculentum var. succenturiatum şi Lycopersicon peruvianum. Varietăţile semiculturale Lycopersicon esculentum var. pruniforme şi mai ales Lycopersicon esculentum var. racemigerum au avut cel mai mic nivel de rezistenţă.[0033] The results obtained demonstrated that, according to the invention, the cultivation of pollen on the selective medium with sodium chloride and the statistical processing of the resistance values of pollen and pollen tubes allow the authentic differentiation of tomato genotypes according to the resistance to salinization based on the integral average resistance. According to the obtained data, maximum resistance to the action of salinization was demonstrated by the Ceros-70 variety, the spontaneous species: Lycopersicon hirsutum gl., Lycopersicon cheesmanii, Lycopersicon chilenzi; then 2 genotypes with a lower level of resistance were highlighted: Lycopersicon esculentum var. succenturiatum and Lycopersicon peruvianum. The semi-cultural varieties Lycopersicon esculentum var. pruniforme and especially Lycopersicon esculentum var. racemigerum had the lowest level of resistance.
[0034] 1. Определение солеустойчивости овощных культур по прорастанию семян в солевых растворах. Методические указания. Ленинград, 1986, 15 c.[0034] 1. Determination of salt tolerance of vegetable crops by seed germination in salt solutions. Methodical instructions. Leningrad, 1986, 15 c.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20090044A MD56Z (en) | 2009-03-31 | 2009-03-31 | Method for selecting salinization-resistant tomato genotypes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20090044A MD56Z (en) | 2009-03-31 | 2009-03-31 | Method for selecting salinization-resistant tomato genotypes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD56Y true MD56Y (en) | 2009-08-31 |
| MD56Z MD56Z (en) | 2010-03-31 |
Family
ID=41120154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20090044A MD56Z (en) | 2009-03-31 | 2009-03-31 | Method for selecting salinization-resistant tomato genotypes |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD56Z (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD653Z (en) * | 2013-02-28 | 2014-02-28 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Method for determination of drought-resistant genotypes of tomato |
-
2009
- 2009-03-31 MD MDS20090044A patent/MD56Z/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD56Z (en) | 2010-03-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Delrot et al. | Genetic and genomic approaches for adaptation of grapevine to climate change | |
| Farooq et al. | Drought stress in wheat during flowering and grain-filling periods | |
| Shelden et al. | Genetic variation in the root growth response of barley genotypes to salinity stress | |
| Dambier et al. | Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry | |
| Heck et al. | Axenic in vitro cultivation of 19 peat moss (Sphagnum L.) species as a resource for basic biology, biotechnology, and paludiculture | |
| Palta et al. | Vigorous crop root systems: form and function for improving the capture of water and nutrients | |
| Fazio | Genetics, breeding, and genomics of apple rootstocks | |
| Muthoni et al. | Reproductive biology and early generation's selection in conventional potato breeding | |
| Guo-Wei et al. | Salt tolerance evaluation of cotton (Gossypium hirsutum) at its germinating and seedling stages and selection of related indices. | |
| CN103571852A (en) | Sea island cotton chromosome segment capable of obviously increasing upland cotton ginning outturn and SSR (simple sequence repeat) marker of sea island cotton chromosome segment | |
| Gailing et al. | Oak population genomics | |
| Van Laere et al. | Woody ornamentals of the temperate zone | |
| US10349611B2 (en) | Heat-tolerant tomato mutant and method for producing the same | |
| DiFazio et al. | Populus: a premier pioneer system for plant genomics | |
| Martínez-Palacios et al. | Genetic diversity of Agave cupreata Trel. & Berger. considerations for its conservation | |
| MD55Y (en) | Method for selecting drought-resistant tomato genotypes | |
| Heuvel | Alnus | |
| MD45Y (en) | Method for selecting tomato genotypes resistant to higher temperatures | |
| CN102517396B (en) | A molecular breeding method for cultivating new varieties of cotton resistant to Verticillium wilt | |
| MD3739F1 (en) | Method for selecting drought resistant tomato genotypes | |
| Rabbi | Marker-Assisted Breeding for Salt Tolerance in Rice: In Bangladesh Context. | |
| MD56Z (en) | Method for selecting salinization-resistant tomato genotypes | |
| Corbière et al. | Do the Algerian Phytophthora infestans populations show genotypic structuration on potato and tomato | |
| Patel | Winter School on Water Productivity Enhancement in Scarcity Zones: Approaches and Applications | |
| Blois | Genetic architecture of root related traits and scion responses induced by grapevine rootstock: First study in a monospecies population of Vitis berlandieri |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| KA4Y | Short-term patent lapsed due to non-payment of fees (with right of restoration) |