MD508Z - Process for presowing treatment of grape seeds - Google Patents
Process for presowing treatment of grape seeds Download PDFInfo
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- MD508Z MD508Z MDS20120006A MDS20120006A MD508Z MD 508 Z MD508 Z MD 508Z MD S20120006 A MDS20120006 A MD S20120006A MD S20120006 A MDS20120006 A MD S20120006A MD 508 Z MD508 Z MD 508Z
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- 238000000034 method Methods 0.000 title claims abstract description 15
- 235000014787 Vitis vinifera Nutrition 0.000 title claims abstract description 12
- 230000008569 process Effects 0.000 title claims abstract description 11
- 240000006365 Vitis vinifera Species 0.000 title claims description 10
- 235000009754 Vitis X bourquina Nutrition 0.000 title abstract 3
- 235000012333 Vitis X labruscana Nutrition 0.000 title abstract 3
- 239000000725 suspension Substances 0.000 claims abstract description 21
- 241000589152 Azotobacter chroococcum Species 0.000 claims abstract description 10
- 241000589540 Pseudomonas fluorescens Species 0.000 claims abstract description 10
- 238000002791 soaking Methods 0.000 claims abstract description 6
- 238000009331 sowing Methods 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 abstract description 13
- 230000007226 seed germination Effects 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 3
- 241000219095 Vitis Species 0.000 abstract 2
- 239000000243 solution Substances 0.000 description 20
- 230000035784 germination Effects 0.000 description 15
- 244000005700 microbiome Species 0.000 description 7
- 230000000694 effects Effects 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 239000012224 working solution Substances 0.000 description 2
- 241000589151 Azotobacter Species 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 1
- 239000011609 ammonium molybdate Substances 0.000 description 1
- 229940010552 ammonium molybdate Drugs 0.000 description 1
- 235000018660 ammonium molybdate Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000003617 indole-3-acetic acid Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
- 229960001669 kinetin Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000021217 seedling development Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940023877 zeatin Drugs 0.000 description 1
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
Description
Invenţia se referă la agricultură, şi anume la un procedeu de tratare a seminţelor de viţă-de-vie înainte de semănat. The invention relates to agriculture, namely to a process for treating grapevine seeds before sowing.
Reproducerea viţei-de-vie prin seminţe este de mare importanţă în selecţie pentru crearea de noi soiuri hibride. Seminţele de viţă-de-vie au o energie scăzută de germinare ca şi unele specii de arbori şi arbuşti. Există metode speciale de pregătire a seminţelor pentru semănat, în scopul sporirii energiei de germinare şi potenţialului de creştere: înmuierea seminţelor în apă, stratificarea şi distrugerea membranelor dense. Reproduction of grapevines by seeds is of great importance in selection for the creation of new hybrid varieties. Grapevine seeds have a low germination energy, like some species of trees and shrubs. There are special methods of preparing seeds for sowing, in order to increase the germination energy and growth potential: soaking seeds in water, stratification and destruction of dense membranes.
Este cunoscut procedeul de stimulare a potenţialului de creştere a seminţelor prin înmuierea lor în soluţii de microelemente la temperatura camerei timp de 12…24 de ore [1]. The process of stimulating the growth potential of seeds by soaking them in microelement solutions at room temperature for 12…24 hours is known [1].
Plantele care se dezvoltă din aceste seminţe cresc mai repede şi sunt mai rezistente la boli şi dăunători. În calitate de microelemente se utilizează permanganat de potasiu, sulfat de cupru, molibdat de amoniu. Plants that develop from these seeds grow faster and are more resistant to diseases and pests. Potassium permanganate, copper sulfate, ammonium molybdate are used as microelements.
Am încercat să combinăm aceste microelemente într-o soluţie unică pentru înmuierea seminţelor. Rezultatele obţinute, practic, au coincis cu cele prezentate în cea mai apropiată soluţie. We tried to combine these microelements in a unique solution for soaking seeds. The results obtained practically coincided with those presented in the closest solution.
Dezavantajul acestor metode este eficacitatea scăzută şi cheltuielile mari de producţie a materialului săditor. În afară de aceasta, în sistemul agricol integrat, care se aplică pe larg în producere, inclusiv în Moldova, se permite optimizarea nutriţiei prin folosirea numai a unor doze minore de îngrăşăminte minerale. The disadvantage of these methods is the low efficiency and high cost of producing seedlings. In addition, the integrated agricultural system, which is widely used in production, including in Moldova, allows optimizing nutrition by using only minor doses of mineral fertilizers.
Problema pe care o rezolvă invenţia propusă constă în majorarea ratei de germinare a seminţelor de viţă-de-vie şi accelerarea viabilităţii materialului săditor. The problem solved by the proposed invention consists in increasing the germination rate of grapevine seeds and accelerating the viability of the planting material.
Procedeul de tratare a seminţelor de viţă-de-vie înainte de semănat prevede înmuierea seminţelor într-o suspensie de bacterii ale tulpinii Pseudomonas fluorescens CNMN-PsB-04 sau Azotobacter chroococcum CNMN-AzB-01 cu titrul de 107 UFC/ml şi un volum de consum al acesteia, ce constituie 1/3 din greutatea seminţelor, în decurs de 3 ore. The process of treating grapevine seeds before sowing involves soaking the seeds in a suspension of bacteria of the Pseudomonas fluorescens CNMN-PsB-04 or Azotobacter chroococcum CNMN-AzB-01 strain with a titer of 107 CFU/ml and a consumption volume thereof, which constitutes 1/3 of the seed weight, within 3 hours.
Rezultatul constă în majorarea energiei de germinare a seminţelor şi intensificarea procesului de creştere a materialului săditor. The result is an increase in seed germination energy and an intensification of the growth process of the planting material.
Tulpini bacteriene din colecţie sunt cultivate timp de 2 zile în mediu de nitrat de lichid sintetic la 27°C până la un titru de 101° UFC/ml. Titrul se stabileşte după metoda larg acceptată de diluţie (Бельтюкова К.И., Матышевская М.С. Методы исследования возбудителей бактериальных болезней, 1960, Киев). Obţinerea soluţiei de lucru: 1 ml de suspensie concentrată, cu un titru de 101° UFC/ml, se toarnă într-un litru de apă şi se obţine o soluţie de lucru cu un titru de 107 UFC/ml. Bacterial strains from the collection are grown for 2 days in synthetic liquid nitrate medium at 27°C to a titer of 101° UFC/ml. The titer is determined by the widely accepted dilution method (Bel'tyukova K.I., Matyshevskaya M.S. Methods of investigation of bacterial pathogens, 1960, Kiev). Preparation of the working solution: 1 ml of concentrated suspension with a titer of 101° UFC/ml is poured into one liter of water and a working solution with a titer of 107 UFC/ml is obtained.
Suspensia de microorganisme reprezintă un mediu de cultură cu celule de bacterii vii, cu titrul iniţial de 101° UFC/ml, şi conţine produsele funcţiilor vitale ale microorganismelor - aminoacizi, vitamine, acid indoleacetic, zeatină, kinetină ş.a. Bacteriile tulpinilor Azotobacter chroococcum CNMN-AzB-01 şi Pseudomonas fluorescens CNMN-PsB-04 au fost utilizate separat. The suspension of microorganisms represents a culture medium with live bacterial cells, with an initial titer of 101° CFU/ml, and contains the products of the vital functions of microorganisms - amino acids, vitamins, indoleacetic acid, zeatin, kinetin, etc. The bacteria of the strains Azotobacter chroococcum CNMN-AzB-01 and Pseudomonas fluorescens CNMN-PsB-04 were used separately.
Exemple de realizare Examples of implementation
Experimentele au fost efectuate în condiţii controlate în complexul de vegetaţie al Institutului de Genetică şi Fiziologie a Plantelor şi în Pepiniera Institutului de Horticultură şi Tehnologii Alimentare. Ca obiect de studiu au servit 2 tipuri de seminţe de viţă-de-vie: de soiul raionat Moldova şi de un hibrid obţinut după încrucişarea soiurilor Moldova x Madlen Angevin. Înainte de semănat în decurs de 3 ore o parte de seminţe a fost înmuiată în suspensie de bacterii ale tulpinii Pseudomonas fluorescens CNMN-PsB-04 (varianta I), altă parte a fost înmuiată în suspensie de bacterii ale tulpinii Azotobacter chroococcum CNMN-AzB-01 (varianta II). Titrul de lucru al suspensiei fiind de 107 UFC/ml, volumul de consum al acesteia constituie 1/3 din greutatea seminţelor. Pentru comparaţie a treia parte de seminţe a fost înmuiată într-o soluţie apoasă de microelemente: Mn+Mo+Cu+B în raportul 1:0,001:0,22:0,38 (cea mai apropiată soluţie). În calitate de martor au servit seminţele tratate cu apă. Evidenţa finală a gradului de germinare a seminţelor a fost efectuată peste 30 de zile după semănat. În perioada de vegetaţie au fost determinaţi următorii indici: cantitatea seminţelor care au germinat, % de germinare, indicele principal al calităţii puieţilor - diametrul lăstarului principal în cm şi % faţă de martor. The experiments were carried out under controlled conditions in the vegetation complex of the Institute of Plant Genetics and Physiology and in the Nursery of the Institute of Horticulture and Food Technologies. The object of study was 2 types of grapevine seeds: the Moldova variety and a hybrid obtained by crossing the Moldova x Madlen Angevin varieties. Before sowing, within 3 hours, one part of the seeds was soaked in a suspension of bacteria of the Pseudomonas fluorescens strain CNMN-PsB-04 (variant I), another part was soaked in a suspension of bacteria of the Azotobacter chroococcum strain CNMN-AzB-01 (variant II). The working titer of the suspension being 107 CFU/ml, its consumption volume is 1/3 of the weight of the seeds. For comparison, the third part of the seeds was soaked in an aqueous solution of microelements: Mn+Mo+Cu+B in the ratio 1:0.001:0.22:0.38 (the closest solution). Seeds treated with water served as a control. The final evidence of the degree of seed germination was carried out over 30 days after sowing. During the vegetation period, the following indices were determined: the amount of seeds that germinated, % germination, the main index of seedling quality - the diameter of the main shoot in cm and % compared to the control.
Exemplul 1 Example 1
În experiment a fost studiat rolul suspensiilor de microorganisme la germinarea seminţelor de soiuri hibride obţinute după încrucişarea soiurilor Moldova x Madlen Angevin. După datele obţinute, care sunt prezentate în tabelul 1, se manifestă un efect pozitiv al microorganismelor asupra germinării seminţelor. Cel mai bun rezultat a fost obţinut după tratarea seminţelor cu suspensie de bacterii ale tulpinii Pseudomonas fluorescens. În această variantă gradul de germinare constituie 74,2%, ce reprezintă 285,4% faţă de martor şi 108,3% faţă de cea mai apropiată soluţie; germinarea seminţelor tratate cu suspensie de bacterii ale tulpinii Azotobacter chroococcum a constituit 71,4% faţă de martor şi 104,2% faţă de cea mai apropiată soluţie. The experiment studied the role of suspensions of microorganisms in the germination of seeds of hybrid varieties obtained after crossing the Moldova x Madlen Angevin varieties. According to the data obtained, which are presented in Table 1, a positive effect of microorganisms on seed germination is manifested. The best result was obtained after treating seeds with a suspension of bacteria of the Pseudomonas fluorescens strain. In this variant, the germination rate is 74.2%, which is 285.4% compared to the control and 108.3% compared to the closest solution; the germination of seeds treated with a suspension of bacteria of the Azotobacter chroococcum strain was 71.4% compared to the control and 104.2% compared to the closest solution.
Tratarea seminţelor conform procedeului propus contribuie la creşterea şi dezvoltarea mai intensivă a plantulelor. La sfârşitul perioadei de vegetaţie lungimea medie a lăstarilor la plantele tratate cu suspensia de bacterii ale tulpinii Pseuromonas fluorescens este de 72,9 cm, ce constituie 215,7% faţă de martor şi 203,6% faţă de cea mai apropiată soluţie. Diametrul mediu al lăstarului principal este cu 180% mai mare faţă de martor şi cu 150% faţă de cea mai apropiată soluţie. În varianta de tratare cu suspensie de bacterii ale tulpinii Azotobacter chroococum lungimea medie a lăstarilor constituie 124,6 şi, respectiv, 117,6%, iar diametrul mediu al lăstarului principal este de 150 şi, respectiv, 125%. Aceasta demonstrează viabilitatea mai sporită a materialului săditor. Seed treatment according to the proposed procedure contributes to more intensive growth and development of seedlings. At the end of the vegetation period, the average length of shoots in plants treated with the suspension of bacteria of the Pseuromonas fluorescens strain is 72.9 cm, which is 215.7% compared to the control and 203.6% compared to the closest solution. The average diameter of the main shoot is 180% larger than the control and 150% compared to the closest solution. In the treatment variant with the suspension of bacteria of the Azotobacter chroococum strain, the average length of shoots is 124.6 and, respectively, 117.6%, and the average diameter of the main shoot is 150 and, respectively, 125%. This demonstrates the increased viability of the seedlings.
Exemplul 2 Example 2
În experimentul 2 a fost studiat efectul suspensiilor de microorganisme asupra germinării seminţelor de soiul Moldova. Datele, prezentate în tabelul 2, confirmă efectul benefic al tulpinilor Pseudomonas fluorescens şi Azotobacter chroococcum asupra germinării seminţelor faţă de martor şi indică rolul pozitiv al microorganismelor în procesul de germinare şi creştere a puieţilor. De menţionat că spre deosebire de seminţele hibride obţinute după încrucişare, pentru seminţe de soiuri cel mai bun efect a fost obţinut în varianta cu Azotobacter chroococcum: germinarea seminţelor a crescut cu 503,0% faţă de martor şi cu 233,7% faţă de cea mai apropiată soluţie. La tratarea seminţelor cu suspensie de bacterii ale tulpinii Pseudomonas fluorescens gradul de germinare constituie 8,6%, ce reprezintă 260,6% faţă de martor şi 121,1% faţă de cea mai apropiată soluţie. Sporirea diametrului mediu al lăstarului principal în varianta de tratare cu suspensie de bacterii ale tulpinii Azotobacter chroococcum constituie 150% faţă de martor şi 130,4% faţă de cea mai apropiată soluţie; în varianta de tratare cu suspensie de bacterii ale tulpinii Pseudomonas fluorescens - respectiv 135 şi 117,4%. Mecanismul acţiunii tulpinilor de microorganisme asupra proceselor menţionate poate fi explicat prin faptul că bacteriile formează în sol (zona rizosferică) un fon microbiologic favorabil, care inactivează dezvoltarea ciupercilor patogene şi sporesc formarea substanţelor biologic active, care accelerează încolţirea seminţelor. In experiment 2, the effect of suspensions of microorganisms on the germination of seeds of the Moldova variety was studied. The data, presented in table 2, confirm the beneficial effect of the Pseudomonas fluorescens and Azotobacter chroococcum strains on the germination of seeds compared to the control and indicate the positive role of microorganisms in the germination and growth of seedlings. It should be noted that, unlike hybrid seeds obtained after crossing, for seeds of varieties, the best effect was obtained in the variant with Azotobacter chroococcum: seed germination increased by 503.0% compared to the control and by 233.7% compared to the closest solution. When treating seeds with a suspension of bacteria of the Pseudomonas fluorescens strain, the germination rate was 8.6%, which represents 260.6% compared to the control and 121.1% compared to the closest solution. The increase in the average diameter of the main shoot in the treatment variant with a suspension of bacteria of the Azotobacter chroococcum strain is 150% compared to the control and 130.4% compared to the closest solution; in the treatment variant with a suspension of bacteria of the Pseudomonas fluorescens strain - 135 and 117.4%, respectively. The mechanism of action of the strains of microorganisms on the mentioned processes can be explained by the fact that the bacteria form a favorable microbiological background in the soil (rhizospheric zone), which inactivates the development of pathogenic fungi and increases the formation of biologically active substances, which accelerate seed germination.
Aşadar, procedeul propus în invenţie - tratarea seminţelor cu suspensii de bacterii ale tulpinii Pseudomonas fluorescens (pentru seminţe obţinute după încrucişare) şi cu suspensie de bacterii ale tulpinii Azotobacter chroococcum (pentru seminţele soiurilor standarde) înainte de semănat - contribuie mai efectiv la germinarea seminţelor de viţă-de-vie şi la creşterea mai sporită a puieţilor comparativ cu martorul şi cea mai apropiată soluţie. Therefore, the process proposed in the invention - treating seeds with bacterial suspensions of the Pseudomonas fluorescens strain (for seeds obtained after crossing) and with bacterial suspension of the Azotobacter chroococcum strain (for seeds of standard varieties) before sowing - contributes more effectively to the germination of grapevine seeds and to the increased growth of seedlings compared to the control and the closest solution.
Tabelul 1 Table 1
Efectul suspensiilor bacteriene asupra germinării seminţelor hibride de viţă-de-vie, obţinute după încrucişare (Moldova x Madlen Angevin) The effect of bacterial suspensions on the germination of hybrid grapevine seeds obtained after crossing (Moldova x Madlen Angevin)
Variante Seminţe semănate Seminţe germi- nate Germi- nare, % % faţă de martor % faţă de cea mai apropiată soluţie Lungimea medie a lăstarilor, cm % faţă de martor % faţă de cea mai apropiată soluţie Diametrul lăstarului principal, cm % faţă de martor % faţă de cea mai apropiată soluţie Martor 50 13 26 100 33,8 100 0,10 100 Cea mai apropiată soluţie 35 24 68,5 263,5 100 35,8 105,9 100 0,12 120,0 100 Varianta I 35 26 74,2 285,4 108,3 72,9 215,7 203,6 0,18 180,0 150 Varianta II 35 25 71,4 274,6 104,2 42,1 124,6 117,6 0,15 150,0 125Variants Seeds sown Seeds germinated Germination, % % compared to control % compared to the closest solution Average shoot length, cm % compared to control % compared to the closest solution Main shoot diameter, cm % compared to control % compared to the closest solution Control 50 13 26 100 33.8 100 0.10 100 Closest solution 35 24 68.5 263.5 100 35.8 105.9 100 0.12 120.0 100 Variant I 35 26 74.2 285.4 108.3 72.9 215.7 203.6 0.18 180.0 150 Variant II 35 25 71.4 274.6 104.2 42.1 124.6 117.6 0.15 150.0 125
Tabelul 2 Table 2
Efectul suspensiilor bacteriene asupra germinării seminţelor şi dezvoltarea puieţilor soiului Moldova The effect of bacterial suspensions on seed germination and seedling development of the Moldova variety
Variante Germi-nare, % % faţă de martor % faţă de cea mai apropiată soluţie Lungimea medie a lăstarilor, cm % faţă de martor % faţă de cea mai apropiată soluţie Diametrul mediu al lăstarului principal, cm % faţă de martor % faţă de cea mai apropiată soluţie Martor 3,3 100 29,0 100 0,2 100 Cea mai apropiată soluţie 7,1 215,2 100 25,8 89,0 100 0,23 115 100 Varianta I 8,6 260,6 121,1 37,2 128,3 144,2 0,27 135 117,4 Varianta II 16,6 503,0 233,7 25,8 89,0 100 0,30 150,0 130,4Germination Variants, % % compared to control % compared to the closest solution Average shoot length, cm % compared to control % compared to the closest solution Average diameter of the main shoot, cm % compared to control % compared to the closest solution Control 3.3 100 29.0 100 0.2 100 Closest solution 7.1 215.2 100 25.8 89.0 100 0.23 115 100 Variant I 8.6 260.6 121.1 37.2 128.3 144.2 0.27 135 117.4 Variant II 16.6 503.0 233.7 25.8 89.0 100 0.30 150.0 130.4
1. Занков З.Д. Результати на предпосевно третирание на лозови семена сьс стимулиращи вещества. Известия. Института по биология "Методий Попов", София, 1962, том 12, с.15-18 1. Занков З.Д. Results of pre-sowing treatment of vine seeds with stimulating substances. Izvestia. Institute of Biology "Methodiy Popov", Sofia, 1962, volume 12, pp. 15-18
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| MD1163G2 (en) * | 1997-07-02 | 1999-10-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Process for presowing carrots seeds treetment |
| RU2154943C2 (en) * | 1994-01-24 | 2000-08-27 | АграСол Инк. | Strain pseudomonas fluorescens designated for control of phytopathogenic fungi (variants), method of its isolation and use, composition containing this strain and method of its use |
| RU2170510C2 (en) * | 1999-07-01 | 2001-07-20 | Совхоз "Тепличный" | Method of preparing biological preparation "ekofit" for protection of plants against phytopathogens and for harvest increase |
| MD2112G2 (en) * | 2001-12-28 | 2003-09-30 | Научно-исследовательский конструкторско-технологический институт табака и табачных изделий | Process for stimulating the tobacco seed germination |
| UA65639C2 (en) * | 2001-05-04 | 2004-04-15 | Інженерно-Технологічний Інститут "Біотехніка" | “bioget” presowing seed and plant treatment and a method of obtaining thereof |
| RU2235771C2 (en) * | 2002-05-13 | 2004-09-10 | Государственное унитарное предприятие "Государственный научный центр прикладной микробиологии" | Strain of bacterium pseudomonas fluorescens p469 for preparing preparation against plant diseases caused by phytopathogenic fungi and microorganisms |
-
2012
- 2012-01-06 MD MDS20120006A patent/MD508Z/en not_active IP Right Cessation
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD170C2 (en) * | 1987-05-14 | 1995-07-31 | Государственный Университет Молд0 | Process for presowing treatment of pumpkin seeds |
| RU2104645C1 (en) * | 1991-04-11 | 1998-02-20 | Зенека Лимитед | Antifungal agent, antifungal agriculture composition, method of inhibition of fungal damage in plants |
| RU2154943C2 (en) * | 1994-01-24 | 2000-08-27 | АграСол Инк. | Strain pseudomonas fluorescens designated for control of phytopathogenic fungi (variants), method of its isolation and use, composition containing this strain and method of its use |
| MD1163G2 (en) * | 1997-07-02 | 1999-10-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Process for presowing carrots seeds treetment |
| RU2170510C2 (en) * | 1999-07-01 | 2001-07-20 | Совхоз "Тепличный" | Method of preparing biological preparation "ekofit" for protection of plants against phytopathogens and for harvest increase |
| UA65639C2 (en) * | 2001-05-04 | 2004-04-15 | Інженерно-Технологічний Інститут "Біотехніка" | “bioget” presowing seed and plant treatment and a method of obtaining thereof |
| MD2112G2 (en) * | 2001-12-28 | 2003-09-30 | Научно-исследовательский конструкторско-технологический институт табака и табачных изделий | Process for stimulating the tobacco seed germination |
| RU2235771C2 (en) * | 2002-05-13 | 2004-09-10 | Государственное унитарное предприятие "Государственный научный центр прикладной микробиологии" | Strain of bacterium pseudomonas fluorescens p469 for preparing preparation against plant diseases caused by phytopathogenic fungi and microorganisms |
Non-Patent Citations (1)
| Title |
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| Занков З.Д. Результати на предпосевно третирание на лозови семена сьс стимулиращи вещества. Известия. Института по биология "Методий Попов", София, 1962, том 12, с.15-18 * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD508Y (en) | 2012-05-31 |
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| KA4Y | Short-term patent lapsed due to non-payment of fees (with right of restoration) |