MD652Z - Process for cultivation of grapevine seedlings - Google Patents
Process for cultivation of grapevine seedlingsInfo
- Publication number
- MD652Z MD652Z MDS20120136A MDS20120136A MD652Z MD 652 Z MD652 Z MD 652Z MD S20120136 A MDS20120136 A MD S20120136A MD S20120136 A MDS20120136 A MD S20120136A MD 652 Z MD652 Z MD 652Z
- Authority
- MD
- Moldova
- Prior art keywords
- treatment
- shoots
- cnmn
- soil
- aqueous solution
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 19
- 235000014787 Vitis vinifera Nutrition 0.000 title 1
- 240000006365 Vitis vinifera Species 0.000 title 1
- 238000005520 cutting process Methods 0.000 claims abstract description 22
- 239000002689 soil Substances 0.000 claims abstract description 15
- 239000000725 suspension Substances 0.000 claims abstract description 14
- 239000007864 aqueous solution Substances 0.000 claims abstract description 10
- 241000589152 Azotobacter chroococcum Species 0.000 claims abstract description 9
- 241000589540 Pseudomonas fluorescens Species 0.000 claims abstract description 9
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 6
- 241000589774 Pseudomonas sp. Species 0.000 claims abstract description 4
- 230000001580 bacterial effect Effects 0.000 claims abstract description 3
- 244000005700 microbiome Species 0.000 claims description 13
- 239000000243 solution Substances 0.000 claims description 13
- 235000015097 nutrients Nutrition 0.000 claims description 6
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 3
- 229910052564 epsomite Inorganic materials 0.000 claims description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 3
- 239000001509 sodium citrate Substances 0.000 claims description 3
- 235000010344 sodium nitrate Nutrition 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 238000010348 incorporation Methods 0.000 abstract description 3
- 238000004448 titration Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 22
- 238000011161 development Methods 0.000 description 11
- 230000004720 fertilization Effects 0.000 description 10
- 241000589516 Pseudomonas Species 0.000 description 7
- 241000589151 Azotobacter Species 0.000 description 6
- 239000003876 biosurfactant Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000000243 photosynthetic effect Effects 0.000 description 4
- 239000000049 pigment Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 239000013543 active substance Substances 0.000 description 2
- 235000021466 carotenoid Nutrition 0.000 description 2
- 150000001747 carotenoids Chemical class 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- 239000001752 chlorophylls and chlorophyllins Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000009369 viticulture Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Invenţia se referă la agricultură, şi anume la un procedeu de cultivare a butaşilor de viţă-de-vie.Procedeul, conform invenţiei, include încorporarea în sol înainte de plantare a suspensiei de bacterii ale tulpinilor Azotobacter chroococcum CNMN-AzB-01 şi Pseudomonas fluorescens CNMN-PsB-04 cu un titru de 107 UFC/ml la un volum de consum al acesteia de 100 ml la 1 kg de sol, tratarea extraradiculară a lăstarilor după formarea a 5 frunze adevărate şi peste 3 şi 6 săptămâni de la prima tratare cu o soluţie apoasă de 0,15% a unui complex de microelemente Mn, Fe, Zn, B, Mo, Co, luate în raport de 1:1,95:0,23:0,44:0,001:0,001 corespunzător, şi o soluţie apoasă de 0,1% de biopreparat, obţinut la cultivarea tulpinii Pseudomonas Sp. PS-17, luate în raport de 1:1, totodată tratarea se efectuează cu un consum total de 0,3…0,5 L/tufă.Rezultatul constă în sporirea creşterii sistemului radicular şi a lăstarilor, precum şi majorarea calităţii butaşilor.The invention relates to agriculture, namely to a process for growing the vines. The process according to the invention includes incorporation into the soil before planting the bacterial suspension of the strains Azotobacter chroococcum CNMN-AzB-01 and Pseudomonas fluorescens CNMN-PsB-04 with a titration of 107 CFU / ml at a consumption volume of 100 ml per 1 kg of soil, extraradicular treatment of shoots after the formation of 5 true leaves and over 3 and 6 weeks after the first treatment with a 0.15% aqueous solution of a Mn, Fe, Zn, B, Mo, Co microelement complex, taken in a ratio of 1: 1.95: 0.23: 0.44: 0.001: 0.001, and a 0.1% aqueous solution of biopreparate obtained from Pseudomonas sp. PS-17, taken in a ratio of 1: 1, at the same time the treatment is carried out with a total consumption of 0.3 ... 0.5 L / bush. The result is the increase of the root system and the shoots, as well as the increase of the quality of the cuttings.
Description
Invenţia se referă la agricultură, şi anume la un procedeu de cultivare a butaşilor de viţă-de-vie. The invention relates to agriculture, namely to a method of growing vine cuttings.
Pepinieritul este una dintre principalele direcţii de dezvoltare şi restabilire a viticulturii în Moldova. Calitatea materialului săditor determină viabilitatea şi înrădăcinarea viţei de vie după plantarea în teren, puterea de creştere, termenul intrării pe rod, productivitatea şi durata de exploatare a plantaţiei. The nursery is one of the main directions of development and restoration of viticulture in Moldova. The quality of the planting material determines the viability and rooting of the vine after planting in the field, the power of growth, the period of fruiting, the productivity and the duration of exploitation of the plantation.
Este cunoscut procedeul de îmbunătăţire a calităţii butaşilor viticoli cu dezvoltarea mai sporită a sistemului radicular şi formarea părţii aeriene a butaşilor [1]. The process of improving the quality of viticultural cuttings with the increased development of the root system and the formation of the aerial part of the cuttings is known [1].
Procedeul constă în bacterizarea butaşilor viţei-de-vie prin încorporarea în sol a suspensiei de tulpini de microorganisme (Azotobacter chroococcum + Pseudomonas fluorescens) şi fertilizarea extraradiculară ulterioară cu microfertilizant Microcom-V (soluţie apoasă de 0,15%) de 3 ori pe parcursul perioadei de vegetaţie (prima stropire - după formarea a 5 frunze adevărate, următoarele două - la un interval de 3 săptămâni). Microcom-V reprezintă un complex de microelemente Mn, Fe, Zn, B, Mo, Co luate în raport de 1:1,95:0,23:0,44:0,001:0,001 corespunzător. Utilizarea microorganismelor şi microelementelor are un impact stimulator asupra proceselor de creştere şi dezvoltare a butaşilor viticoli, în special asupra procesului de formare a sistemului radicular. The procedure consists in the bacterialization of the vine cuttings by incorporating the suspension of strains of microorganisms (Azotobacter chroococcum + Pseudomonas fluorescens) into the soil and the subsequent extraradicular fertilization with Microcom-V microfertilizer (0.15% aqueous solution) 3 times during the vegetation period (the first spraying - after the formation of 5 true leaves, the next two - at an interval of 3 weeks). Microcom-V represents a complex of microelements Mn, Fe, Zn, B, Mo, Co taken in the corresponding 1:1.95:0.23:0.44:0.001:0.001 ratio. The use of microorganisms and microelements has a stimulating impact on the processes of growth and development of vine cuttings, especially on the process of forming the root system.
Dezavantajul acestui procedeu este faptul că frunzele sunt fertilizate cu soluţii cu metaboliţi microbieni şi microelemente, care în condiţii de secetă se evaporă rapid sau soluţia nutritivă se scurge de pe frunze. Eficacitatea tratamentului din aceste cauze este redusă. Este cunoscut faptul că utilizarea de agenţi superficial activi (surfactanţi) sporeşte eficienţa fertilizării extraradiculare datorită fixării nutrienţilor pe suprafata frunzelor. Surfactanţii acţionează ca adezivi, regulatori ai proceselor de umezire şi de transport de substanţe active vizate la celulele şi ţesuturile plantelor, activatori de sisteme de enzime. Pornind de la necesitatea de a înlocui în condiţiile agriculturii integrate preparatele chimice cu cele de provenienţă biologică, sunt elaboraţi biosurfactanţi, care sunt mai eficienţi şi ecologic inofensivi faţă de preparatele chimice, în special un producent nou al microorganismelor tulpinii Pseudomonas (UA 71792 A 2004.12.15). The disadvantage of this process is the fact that the leaves are fertilized with solutions with microbial metabolites and microelements, which in drought conditions evaporate quickly or the nutrient solution drains from the leaves. The effectiveness of treatment from these causes is reduced. It is known that the use of superficially active agents (surfactants) increases the efficiency of extraradicular fertilization due to the fixation of nutrients on the surface of the leaves. Surfactants act as adhesives, regulators of wetting processes and transport of active substances aimed at plant cells and tissues, activators of enzyme systems. Starting from the need to replace chemical preparations with those of biological origin in the conditions of integrated agriculture, biosurfactants are developed, which are more efficient and ecologically harmless compared to chemical preparations, especially a new producer of microorganisms of the Pseudomonas strain (UA 71792 A 2004.12. 15).
Biosurfactantul se produce conform prescripţiilor tehnice ТУ У 24.5-32613446-004:2004 de ТОВ ”Дослідний завод Граніт” (S.R.L ”Uzina Experimentală Granit”) cu denumirea ”Полiком”. Biosurfactantul reprezintă un biopreparat superficial activ, ce conţine producentul bacteriilor tulpinii Pseudomonas Sp. PS-17, cultivată pe un mediu nutritiv ce conţine, g/L: NaNO3 2,0…4,0; K2HPO4·3H2O 1,5…2,5; KHPO4 1,0…1,5; MgSO4·7H2O 0,2…0,7; citrat de sodiu 2,0…5,0 şi un substrat de creştere hidrosolubil, insolubil sau un amestec al acestora, 10,0…30,0, la un PH de 6,8…7,2, temperatura de 28…32°C în decurs de 2…7 zile. După cultivare din mediul nutritiv se separă microorganismele, iar compoziţia obţinută reprezintă un biocomplex superficial activ, adică biosurfactantul menţionat, care se utilizează în formă concentrată cu un conţinut de cel puţin 50% mas. substanţe uscate. The biosurfactant is produced according to the technical prescriptions ТУ У 24.5-32613446-004:2004 by TOV "Дослидний завод Гранит" (S.R.L "Experimental Granit Plant") under the name "Поликом". The biosurfactant is a superficially active biopreparation, which contains the producer of bacteria of the strain Pseudomonas Sp. PS-17, cultivated on a nutrient medium containing, g/L: NaNO3 2.0...4.0; K2HPO4·3H2O 1.5...2.5; KHPO4 1.0...1.5; MgSO4·7H2O 0.2...0.7; sodium citrate 2.0...5.0 and a water-soluble, insoluble growth substrate or a mixture thereof, 10.0...30.0, at a PH of 6.8...7.2, temperature of 28...32° C within 2…7 days. After cultivation, the microorganisms are separated from the nutrient medium, and the obtained composition represents a superficially active biocomplex, i.e. the mentioned biosurfactant, which is used in concentrated form with a content of at least 50% by mass. dry substances.
Problema pe care o rezolvă invenţia propusă constă în sporirea efectului de prelucrare a butaşilor viţei-de-vie cu microelemente şi suspensii microbiene prin adăugarea biosurfactantului pentru a spori calitatea şi cantitatea materialului săditor al viţei-de-vie. The problem that the proposed invention solves is to increase the effect of processing vine cuttings with microelements and microbial suspensions by adding the biosurfactant to increase the quality and quantity of the vine planting material.
Procedeul de cultivare a butaşilor de viţă-de-vie include încorporarea în sol înainte de plantare a suspensiei de bacterii ale tulpinilor Azotobacter chroococcum CNMN-AzB-01 şi Pseudomonas fluorescens CNMN-PsB-04 cu un titru de 107 UFC/ml la un volum de consum al acesteia de 100 ml la 1 kg de sol, tratarea extraradiculară a lăstarilor după formarea a 5 frunze adevărate şi peste 3 şi 6 săptămâni de la prima tratare cu o soluţie apoasă de 0,15% a unui complex de microelemente Mn, Fe, Zn, B, Mo, Co, luate în raport de 1:1,95:0,23:0,44:0,001:0,001 corespunzător, şi o soluţie apoasă de 0,1% de biopreparat, obţinut la cultivarea tulpinii Pseudomonas Sp. PS-17, pe un mediu nutritiv ce conţine, g/L: NaNO3 2,0…4,0; K2HPO4·3H2O 1,5…2,5; KHPO4 1,0…1,5; MgSO4·7H2O 0,2…0,7; citrat de sodiu 2,0…5,0 şi un substrat de creştere hidrosolubil sau insolubil, sau un amestec al acestora, 10,0…30,0, la un PH de 6,8…7,2, temperatura de 28…32°C în decurs de 2…7 zile, cu separarea microorganismelor şi concentrarea ulterioară a mediului de cultură până la un conţinut de cel puţin 50% mas. substanţe uscate, totodată soluţiile sunt luate în raport de 1:1, iar tratarea se efectuează cu un consum total de 0,3…0,5 L/tufă. The process of growing vine cuttings includes incorporating into the soil before planting the bacterial suspension of the strains Azotobacter chroococcum CNMN-AzB-01 and Pseudomonas fluorescens CNMN-PsB-04 with a titer of 107 CFU/ml at a volume of its consumption of 100 ml per 1 kg of soil, extraradicular treatment of the shoots after the formation of 5 true leaves and over 3 and 6 weeks after the first treatment with a 0.15% aqueous solution of a complex of microelements Mn, Fe . . PS-17, on a nutrient medium containing, g/L: NaNO3 2.0...4.0; K2HPO4·3H2O 1.5...2.5; KHPO4 1.0...1.5; MgSO4·7H2O 0.2...0.7; sodium citrate 2.0...5.0 and a water-soluble or insoluble growth substrate, or a mixture thereof, 10.0...30.0, at a PH of 6.8...7.2, temperature of 28...32 °C within 2...7 days, with the separation of microorganisms and subsequent concentration of the culture medium up to a content of at least 50% mass. dry substances, also the solutions are taken in a ratio of 1:1, and the treatment is carried out with a total consumption of 0.3...0.5 L/bush.
Raportul soluţiilor de microelemente şi de biopreparat este de 1:1 (se recomandă de a fi amestecate înainte de stropire). The ratio of the solutions of microelements and biopreparation is 1:1 (it is recommended to be mixed before spraying).
Rezultatul constă în sporirea creşterii sistemului radicular şi a lăstarilor, precum şi majorarea calităţii butaşilor. The result consists in increasing the growth of the root system and shoots, as well as increasing the quality of the cuttings.
Rezultatul se datorează sporirii cantităţii de pigmenţi fotosintetici în frunzele butaşilor înrădăcinaţi, ceea ce indică o intensificare a proceselor metabolice în plante, ca rezultat sporeşte creşterea sistemului radicular şi a lăstarilor. The result is due to the increase in the amount of photosynthetic pigments in the leaves of the rooted cuttings, which indicates an intensification of the metabolic processes in the plants, as a result increases the growth of the root system and shoots.
Exemplu de realizare Example of realization
Experimentele au fost efectuate în anii 2009-2011 în complexul de vegetaţie al Institutului de Genetică şi Fiziologie a Plantelor, în seră şi pepiniera Institutului de Horticultură şi Tehnologii Alimentare. Ca obiect de studiu au servit 2 soiuri de viţă-de-vie - Codrinschi şi Prezentabelinîi. The experiments were carried out in the years 2009-2011 in the vegetation complex of the Institute of Genetics and Physiology of Plants, in the greenhouse and nursery of the Institute of Horticulture and Food Technologies. 2 vine varieties - Codrinschi and Prezentabelînii - served as the object of study.
Schema experimentelor Scheme of the experiments
1. Bacterizarea butaşilor prin încorporarea în sol a suspensiei tulpinilor Azotobacter chroococcum CNMN-AzB-01 + Pseudomonas fluorescens CNMN-PsB-04 cu fertilizarea extraradiculară ulterioară cu un complex de microelemente Microcom-V şi cu biopreparatul superficial activ produs de bacteriile tulpinii Pseudomonas Sp. PS-17. 1. Bacterization of the cuttings by incorporating the suspension of Azotobacter chroococcum CNMN-AzB-01 + Pseudomonas fluorescens CNMN-PsB-04 strains into the soil with subsequent extraradicular fertilization with a microelement complex Microcom-V and with the superficially active biopreparation produced by the bacteria of the Pseudomonas Sp strain. PS-17.
2. Bacterizarea butaşilor prin încorporarea în sol a suspensiei tulpinilor Azotobacter chroococcum CNMN-AzB-01 + Pseudomonas fluorescens CNMN-PsB-04 cu fertilizarea extraradiculară ulterioară cu complexul de microelemente Microcom-V - cea mai apropiată soluţie. 2. Bacterization of the cuttings by incorporating the suspension of Azotobacter chroococcum CNMN-AzB-01 + Pseudomonas fluorescens CNMN-PsB-04 strains into the soil with subsequent extraradicular fertilization with the microelement complex Microcom-V - the closest solution.
3. Stropirea butaşilor cu apă - martor. 3. Spraying the cuttings with water - witness.
Încorporarea în sol a suspensiei acestor tulpini a fost efectuată înainte de sădirea butaşilor (100 ml de suspensie la 1 kg de sol). Fertilizarea extraradiculară a fost efectuată cu soluţie apoasă a complexului de microelemente Microcom-V, - în concentraţie de 0,15%, şi soluţia apoasă de biopreparat în concentraţie de 0,1%. Termen de fertilizare - de 3 ori pe parcursul perioadei de vegetaţie, după formarea a 5 frunze adevărate şi peste 3 şi 6 săptămâni de la prima tratare. În perioada de vegetaţie au fost determinaţi următorii indici: conţinutul pigmenţilor fotosintetici în frunze, indicele principal al calităţii butaşilor - cantitatea şi masa medie a lăstarilor, în cm şi % faţă de martor, cantitatea, lungimea şi masa rădăcinilor la o plantă. The incorporation of the suspension of these stems into the soil was carried out before planting the cuttings (100 ml of suspension per 1 kg of soil). Extraradicular fertilization was performed with an aqueous solution of the microelement complex Microcom-V, - in a concentration of 0.15%, and the aqueous solution of the biopreparation in a concentration of 0.1%. Fertilization term - 3 times during the vegetation period, after the formation of 5 true leaves and over 3 and 6 weeks after the first treatment. During the vegetation period, the following indices were determined: the content of photosynthetic pigments in the leaves, the main index of the quality of the cuttings - the amount and average mass of the shoots, in cm and % compared to the control, the amount, length and mass of the roots in one plant.
A fost studiat efectul suspensiilor de microorganisme, complexului Microcom-V şi biopreparatului asupra conţinutului pigmenţilor fotosintetici în frunze, precum şi asupra creşterii şi dezvoltării plantelor de soiul Prezentabelinîi în pepiniera viticolă. Datele prezentate în tab. 1 mărturisesc că conţinutul sumar al clorofilelor a+b evident a crescut după încorporarea în sol a tulpinilor Azotobacter chroococcum + Pseudomonas fluorescens şi fertilizarea foliară a plantelor cu Microcom-V + biopreparat (procedeul propus) faţă de martor şi cea mai apropiată soluţie. Conţinutul carotinoidelor s-a micşorat după tratarea plantelor. The effect of microorganism suspensions, the Microcom-V complex and the biopreparation on the content of photosynthetic pigments in leaves, as well as on the growth and development of plants of the Presentabelinii variety in the vineyard nursery, was studied. The data presented in tab. 1 testify that the summary content of chlorophylls a+b obviously increased after the incorporation of Azotobacter chroococcum + Pseudomonas fluorescens strains into the soil and the foliar fertilization of the plants with Microcom-V + biopreparation (the proposed procedure) compared to the control and the closest solution. The content of carotenoids decreased after treating the plants.
Tabelul 1 Table 1
Conţinutul de pigmenţi fotosintetici în frunzele viţei-de-vie (s. Prezentabelinîi) The content of photosynthetic pigments in vine leaves (Prezentabelînii village)
în funcţie de fertilizarea butaşilor, pepiniera viticolă, mg/g la 1 m2 depending on the fertilization of the cuttings, the viticultural nursery, mg/g per 1 m2
Variante Clorof. a Clorof. b Clorof. a+b Carotinoide Martor 1,005±0,5 0,383±0,5 1,388±0,1 0,314±0,00 Cea mai apropiata soluţie: Azotobacter chr.+ Pseudomonas fluor.+ Microcom-V 1,059±0,1 0,450±0,5 1,536±0,1 0,258±0,50 Procedeul propus (invenţie): Azotobacter chr.+ Pseudomonas fluor.+ Microcom-V + biopreparat 1,170±0,7 0,527±0,5 1,697±0,1 0,207±0,60 Variants of Chloroph. of Chloroph. b Chloroph. a+b Control Carotenoids 1.005±0.5 0.383±0.5 1.388±0.1 0.314±0.00 Closest solution: Azotobacter chr.+ Pseudomonas fluor.+ Microcom-V 1.059±0.1 0.450±0. 5 1.536±0.1 0.258±0.50 Proposed process (invention): Azotobacter chr.+ Pseudomonas fluor.+ Microcom-V + biopreparation 1.170±0.7 0.527±0.5 1.697±0.1 0.207±0.60
Tabelul 2 Table 2
Creşterea şi dezvoltarea butaşilor de viţă-de-vie în funcţie de fertilizare Growth and development of vine cuttings depending on fertilization
(media dintre 10 plante), s. Prezentabelinîi (average of 10 plants), Prezentabelînii village
Variante Lăstari Rădăcini Masa medie 1 puiet, g ( lăstari + rădăcini) Lungimea medie, cm/ puiet Masa medie, g/puiet Lungimea medie, cm/puiet Masa medie, g/puiet Martor 27,73±1,76 3,56±0,48 186,56±28,80 3,29±0,63 6,85 Cea mai apropiata soluţie: Azotobacter chr.+ Pseudomonas fluor. + Microcom-V 39,73±21,99 8,47±2,83 237,43±14,78 6,32±1,84 14,79 Procedeul propus (invenţie): Azotobacter chr.+ + Pseudomonas fluor. + Microcom-V + biopreparat 55,53±8,78 8,63±1,05 245,4±37,40 6,51±1,48 15,14 Variants Shoots Roots Average weight 1 seedling, g (shoots + roots) Average length, cm/seedling Average weight, g/seedling Average length, cm/seedling Average weight, g/seedling Control 27.73±1.76 3.56± 0.48 186.56±28.80 3.29±0.63 6.85 The closest solution: Azotobacter chr.+ Pseudomonas fluor. + Microcom-V 39.73±21.99 8.47±2.83 237.43±14.78 6.32±1.84 14.79 Proposed process (invention): Azotobacter chr.+ + Pseudomonas fluor. + Microcom-V + biopreparation 55.53±8.78 8.63±1.05 245.4±37.40 6.51±1.48 15.14
În tab. 2 sunt prezentate datele biometrice - lungimea şi masa lăstarilor şi rădăcinilor la sfârşitul experimentului, care mărturisesc despre efectul pozitiv al tratării plantelor cu microorganisme, microelemente şi cu biopreparat (procedeul propus) faţă de martor şi cea mai apropiata soluţie. Lungimea medie a lăstarilor la un puiet în varianta procedeului propus a constituit 139,8% faţă de cea mai apropiata soluţie, a rădăcinilor - 103,4%. A sporit nu numai lungimea lăstarilor şi a rădăcinilor, dar şi masa medie a puieţilor, ce denotă o dezvoltare mai sporită a plantelor şi o viabilitate mai înaltă a materialului săditor. In tab. 2 shows the biometric data - the length and mass of the shoots and roots at the end of the experiment, which testify to the positive effect of treating plants with microorganisms, microelements and biopreparation (the proposed procedure) compared to the control and the closest solution. The average length of the shoots per seedling in the version of the proposed procedure was 139.8% compared to the closest solution, of the roots - 103.4%. It increased not only the length of the shoots and roots, but also the average mass of the seedlings, which indicates a higher development of the plants and a higher viability of the planting material.
Totodată a fost studiat efectul suspensiilor de microorganisme, complexului Microcom-V şi biopreparat asupra creşterii şi dezvoltării plantelor de soiul Codrinschi în vase cu sol în seră. Acest soi se caracterizează printr-o creştere a lăstarilor mai intensivă şi rizogeneză mai slabă faţă de soiul Prezentabelinîi. În tab. 3 sunt prezentate datele biometrice ale puieţilor la sfârşitul experimentului. Cel mai bun rezultat a fost obţinut după încorporarea în sol a microorganismelor Azotobacter chroococcum + Pseudomonas fluorescens şi tratarea extraradiculară a lăstarilor cu microelemente şi biopreparat. Procedeul propus contribuie la intensificarea creşterii şi a dezvoltării părţii aeriene şi, mai puţin, a sistemului radicular al plantelor. Cantitatea medie a lăstarilor pe o plantă în această variantă constituie 109,2% faţă de martor şi 107,2% faţă de cea mai apropiată soluţie. At the same time, the effect of microorganism suspensions, Microcom-V complex and biopreparation on the growth and development of plants of the Codrinschi variety in pots with soil in the greenhouse was studied. This variety is characterized by a more intensive shoot growth and weaker rhizogenesis compared to the Presentabelinii variety. In tab. 3 shows the biometric data of the seedlings at the end of the experiment. The best result was obtained after incorporating the microorganisms Azotobacter chroococcum + Pseudomonas fluorescens into the soil and extraradicular treatment of the shoots with microelements and biopreparation. The proposed procedure contributes to the intensification of the growth and development of the aerial part and, to a lesser extent, the root system of the plants. The average number of shoots per plant in this variant is 109.2% compared to the control and 107.2% compared to the closest solution.
Tabelul 3 Table 3
Creşterea şi dezvoltarea butaşilor de viţă-de-vie în seră, în funcţie de fertilizare, media dintre 10 plante, s. Codrinschi Growth and development of vine cuttings in the greenhouse, depending on fertilization, average of 10 plants, Codrinschi village
Variante Lăstari Rădăcini Cantitatea medie a lăstarilor/plantă Lungimea medie a lăstarilor/plantă, cm Masa uscată a lăstarilor/plantă, g Masa uscată a rădăcinilor /plantă, g Martor 2,17 179,7 0,9 3,68 Cea mai apropiata soluţie: Azotobacter chr.+ Pseudomonas fluor. + Microcom-V 2,21 203,5 1,20 4,86 Procedeul propus (invenţie): Azotobacter chr.+ Pseudomonas fluor. + Microcom-V + biopreparat 2,37 204,5 1,35 4,91 Variants Shoots Roots Average number of shoots/plant Average length of shoots/plant, cm Dry weight of shoots/plant, g Dry weight of roots/plant, g Control 2.17 179.7 0.9 3.68 Closest solution : Azotobacter chr.+ Pseudomonas fluor. + Microcom-V 2.21 203.5 1.20 4.86 Proposed process (invention): Azotobacter chr. + Pseudomonas fluor. + Microcom-V + biopreparation 2.37 204.5 1.35 4.91
Aşadar, conform procedeului propus, bacterizarea butaşilor prin încorporarea în sol a suspensiei de bacterii ale tulpinilor Azotobacter chroococcum + Pseudomonas fluorescens şi tratarea exraradiculară a plantelor de 3 ori pe perioada de vegetaţie cu soluţie apoasă a complexului de microelemente Microcom-V în concentraţie de 0,15% (jumătate de doză de Microcom-V) cu adăugarea soluţiei apoase de 0,1% de biopreparat contribuie la intensificarea creşterii şi a dezvoltării butaşilor înrădăcinaţi cu obţinerea materialului săditor mai calitativ şi viabil. Principiul de interacţiune efectivă a suspensiilor de microorganisme, a complexului de microelemente şi a biosurfactantului constă în sinergia acţiunii dozelor optime şi a raportului componentelor, accesul activ în rădăcinile plantelor a compuşilor biologici activi din suspensia de microorganisme şi a microelementelor prin stomate. Therefore, according to the proposed procedure, the bacteriization of the cuttings by incorporating in the soil the suspension of bacteria of the Azotobacter chroococcum + Pseudomonas fluorescens strains and the exraradicular treatment of the plants 3 times during the vegetation period with an aqueous solution of the microelement complex Microcom-V in a concentration of 0, 15% (half the dose of Microcom-V) with the addition of the 0.1% aqueous solution of the biopreparation contributes to the intensification of the growth and development of the rooted cuttings with the obtaining of more qualitative and viable planting material. The principle of effective interaction of microorganism suspensions, the microelement complex and the biosurfactant consists in the synergy of the action of optimal doses and the ratio of components, the active access to the plant roots of the active biological compounds from the microorganism suspension and microelements through the stomata.
1. Veliksar S., Lemanova N., David T., Carpenco E., Comaneniuc A., Bratco D. Efectul microelementelor şi microorganismelor asupra creşterii şi dezvoltării butaşilor viţei-de-vie. Chişinău. Conferinţa ştiinţifică AŞM. Genetica şi fiziologia rezistenţei plantelor, 2011, p. 66 1. Veliksar S., Lemanova N., David T., Carpenco E., Comaneniuc A., Bratco D. The effect of microelements and microorganisms on the growth and development of vine cuttings. Chisinau. ASM scientific conference. Genetics and physiology of plant resistance, 2011, p. 66
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| Publication number | Priority date | Publication date | Assignee | Title |
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| MD2654G2 (en) * | 2004-05-13 | 2005-09-30 | Институт Физиологии Растений Академии Наук Республики Молдова | Process for controlling the vine mineral nutrition |
| RU2377227C1 (en) * | 2008-10-23 | 2009-12-27 | Общество с ограниченной ответственностью "Научно-производственная фирма Минерал" | Substance for foliar treatment of agricultural crops |
| RU2401824C2 (en) * | 2008-12-18 | 2010-10-20 | Андрей Геннадиевич Петропавловский | Zelenit fertiliser |
| MD270Z (en) * | 2010-06-04 | 2011-04-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for extraroot fertilization of grapes |
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| MD2654G2 (en) * | 2004-05-13 | 2005-09-30 | Институт Физиологии Растений Академии Наук Республики Молдова | Process for controlling the vine mineral nutrition |
| RU2377227C1 (en) * | 2008-10-23 | 2009-12-27 | Общество с ограниченной ответственностью "Научно-производственная фирма Минерал" | Substance for foliar treatment of agricultural crops |
| RU2401824C2 (en) * | 2008-12-18 | 2010-10-20 | Андрей Геннадиевич Петропавловский | Zelenit fertiliser |
| MD270Z (en) * | 2010-06-04 | 2011-04-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for extraroot fertilization of grapes |
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| Veliksar S., Lemanova N., David T., Carpenco E., Comaneniuc A., Bratco D. Efectul microelementelor şi microorganismelor asupra creşterii şi dezvoltării butaşilor viţei-de-vie. Chişinău. Conferinţa ştiinţifică AŞM. Genetica şi fiziologia rezistenţei plantelor, 2011, p. 66 * |
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| MD845Z (en) * | 2014-05-05 | 2015-07-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for extraroot treatment of vine |
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