MD2823G2 - Process for quantitatively determining the sclareol in the obtained from clary sage - Google Patents
Process for quantitatively determining the sclareol in the obtained from clary sageInfo
- Publication number
- MD2823G2 MD2823G2 MDA20040036A MD20040036A MD2823G2 MD 2823 G2 MD2823 G2 MD 2823G2 MD A20040036 A MDA20040036 A MD A20040036A MD 20040036 A MD20040036 A MD 20040036A MD 2823 G2 MD2823 G2 MD 2823G2
- Authority
- MD
- Moldova
- Prior art keywords
- sclareol
- chloroform
- quantitatively determining
- clary sage
- aluminum oxide
- Prior art date
Links
Landscapes
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The invention relates to the chemistry of natural compounds, namely to a process for quantitatively determining the sclareol in the extracts obtained from clary sage.Summary of the invention consists in that the process includes preparation of a chromatographic plate with the dimensions 10 x 20 cm, with a layer of aluminum oxide of I-st degree of activity, having the trickness of 1,8…2,0 mm, afterwards the weighted sample of extract is dissolved in chloroform, is applied onto the plate at a distance of 1,5 cm from the inferior margin to a length of 7,5 cm of the starting line and is separated by two complete consecutive passé of the solvent system nifras: chloroform: acetone in the corresponding ratio of 5:4:1, then the dried aluminum oxide, containing sclareol, is collected beginning with the distance of 2,5 cm from the starting line and up to 11,5 cm from it, is consecutively extracted with chloroform at 40…45°C with 40, 30, 10 and 10 ml, and after removal of the chloroform the obtained residue is weighted and it is calculated the content of sclareol.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MDA20040036A MD2823G2 (en) | 2004-02-12 | 2004-02-12 | Process for quantitatively determining the sclareol in the obtained from clary sage |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MDA20040036A MD2823G2 (en) | 2004-02-12 | 2004-02-12 | Process for quantitatively determining the sclareol in the obtained from clary sage |
Publications (2)
Publication Number | Publication Date |
---|---|
MD2823F1 MD2823F1 (en) | 2005-07-30 |
MD2823G2 true MD2823G2 (en) | 2006-05-31 |
Family
ID=34859151
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MDA20040036A MD2823G2 (en) | 2004-02-12 | 2004-02-12 | Process for quantitatively determining the sclareol in the obtained from clary sage |
Country Status (1)
Country | Link |
---|---|
MD (1) | MD2823G2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104569215B (en) * | 2015-01-28 | 2016-03-16 | 无锡市人民医院 | A kind of method measuring sclareol concentration in blood plasma |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU807787A1 (en) * | 1979-01-30 | 1995-11-27 | В.Г. Березкин | Method of qualitative and quantitative determination substances in plane chromatography |
RU2082168C1 (en) * | 1994-01-26 | 1997-06-20 | Сибирский ботанический сад при Томском государственном университете | Method of detection and quantitative determination of ecdisteroids in plant objects |
EA199900159A1 (en) * | 1998-06-10 | 1999-12-29 | Де Веллкам Фаундейшн Лимитед | CONNECTION AND ITS APPLICATION |
MD1772G2 (en) * | 1999-09-30 | 2002-04-30 | Государственный Университет Молд0 | Method of sunflower mitochondrions fraction identification |
RU2216018C2 (en) * | 2000-01-17 | 2003-11-10 | Литвинова Лариса Степановна | Method to realize flow thin-layer chromatography and device for its implementation |
-
2004
- 2004-02-12 MD MDA20040036A patent/MD2823G2/en not_active IP Right Cessation
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU807787A1 (en) * | 1979-01-30 | 1995-11-27 | В.Г. Березкин | Method of qualitative and quantitative determination substances in plane chromatography |
RU2082168C1 (en) * | 1994-01-26 | 1997-06-20 | Сибирский ботанический сад при Томском государственном университете | Method of detection and quantitative determination of ecdisteroids in plant objects |
EA199900159A1 (en) * | 1998-06-10 | 1999-12-29 | Де Веллкам Фаундейшн Лимитед | CONNECTION AND ITS APPLICATION |
EA000666B1 (en) * | 1998-06-10 | 2000-02-28 | Де Веллкам Фаундейшн Лимитед | Triazine derivatives and use thereof |
MD1772G2 (en) * | 1999-09-30 | 2002-04-30 | Государственный Университет Молд0 | Method of sunflower mitochondrions fraction identification |
RU2216018C2 (en) * | 2000-01-17 | 2003-11-10 | Литвинова Лариса Степановна | Method to realize flow thin-layer chromatography and device for its implementation |
Non-Patent Citations (3)
Title |
---|
Ахрем А.А., Кузнецова А.И. Тонкослойная хроматография. Наука, Москва, 1965, с. 49 - 54, 112 - 117 * |
Лазурьевский Г.В., Терентьева И. В., Шамшурин А.А. Практические работы по химии природных соединений. Высшая школа, Москва, 1966, с. 153 - 155 * |
Шталь Э. (ред.). Хроматография в тонких слоях. Мир, Москва, 1965, с. 194 - 197 * |
Also Published As
Publication number | Publication date |
---|---|
MD2823F1 (en) | 2005-07-30 |
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Legal Events
Date | Code | Title | Description |
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FG4A | Patent for invention issued | ||
KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
MM4A | Patent for invention definitely lapsed due to non-payment of fees |