MD1772G2 - Method of sunflower mitochondrions fraction identification - Google Patents
Method of sunflower mitochondrions fraction identificationInfo
- Publication number
- MD1772G2 MD1772G2 MD99-0239A MD990239A MD1772G2 MD 1772 G2 MD1772 G2 MD 1772G2 MD 990239 A MD990239 A MD 990239A MD 1772 G2 MD1772 G2 MD 1772G2
- Authority
- MD
- Moldova
- Prior art keywords
- mitochondrions
- fraction
- identification
- sunflower
- allomaleic
- Prior art date
Links
Landscapes
- Compounds Of Unknown Constitution (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention refers to plants physiology and biochemistry and may by used in the laboratories for mitochondrions fraction identification.The method of sunflower mitochondrions fraction identification includes homogenizing the plant material, extracting the mitochondrions fraction of the homogenized medium by the differential centrifugation, washing the mitochondrions in the homogenized cystein free medium. Slurrying of the mitochondrions fraction is executed in a solution containing saccharose and TRIS-HCl buffer. For degradation of the mitochondrions membranes in this solution it is added Triton X-100. The identification of the mitochondrions fraction is executed by the method of chromatography on the sylufolic plates according to appearing of the allomaleic and malic acids zones, formed as a result of mitochondrions enzyms action on the succinic or allomaleic acids accordingly.The result of the invention consists in simplification, acceleration and reducing the price of the mitochondrions fraction identification method.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MD99-0239A MD1772G2 (en) | 1999-09-30 | 1999-09-30 | Method of sunflower mitochondrions fraction identification |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MD99-0239A MD1772G2 (en) | 1999-09-30 | 1999-09-30 | Method of sunflower mitochondrions fraction identification |
Publications (2)
Publication Number | Publication Date |
---|---|
MD1772F1 MD1772F1 (en) | 2001-10-30 |
MD1772G2 true MD1772G2 (en) | 2002-04-30 |
Family
ID=19739467
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MD99-0239A MD1772G2 (en) | 1999-09-30 | 1999-09-30 | Method of sunflower mitochondrions fraction identification |
Country Status (1)
Country | Link |
---|---|
MD (1) | MD1772G2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MD2823G2 (en) * | 2004-02-12 | 2006-05-31 | Институт Химии Академии Наук Молдовы | Process for quantitatively determining the sclareol in the obtained from clary sage |
-
1999
- 1999-09-30 MD MD99-0239A patent/MD1772G2/en unknown
Non-Patent Citations (2)
Title |
---|
Андреева Н.Н. Методы выделения функционально активных митохондрий из растительных тканей. Клетка и клеточные структуры. Москва, 1968, т. 1, с. 65-70 * |
Современные методы анализа в биохимии, под ред. В.Н. Ореховича. Москва, 1977, с. 41-49 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MD2823G2 (en) * | 2004-02-12 | 2006-05-31 | Институт Химии Академии Наук Молдовы | Process for quantitatively determining the sclareol in the obtained from clary sage |
Also Published As
Publication number | Publication date |
---|---|
MD1772F1 (en) | 2001-10-30 |
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