MD2823F1 - Process for quantitatively determining the sclareol in the obtained from clary sage - Google Patents
Process for quantitatively determining the sclareol in the obtained from clary sageInfo
- Publication number
- MD2823F1 MD2823F1 MDA20040036A MD20040036A MD2823F1 MD 2823 F1 MD2823 F1 MD 2823F1 MD A20040036 A MDA20040036 A MD A20040036A MD 20040036 A MD20040036 A MD 20040036A MD 2823 F1 MD2823 F1 MD 2823F1
- Authority
- MD
- Moldova
- Prior art keywords
- sclareol
- chloroform
- plate
- aluminum oxide
- distance
- Prior art date
Links
Landscapes
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
Inventia se refera la chimia compusilor naturali si anume la un procedeu de determinare cantitativa a sclareolului in extractele, obtinute din salvia de muscat. Esenta inventiei consta in aceea ca procedeul include pregatirea unei placi cromatografice cu dimensiunile 10 x 20 cm, cu un strat de oxid de aluminiu cu gradul I de activitate avand grosimea de 1,8...2,0 mm, dupa care proba cantarita de extract se dizolva in cloroform, se aplica pe placa la distanta de 1,5 cm de la marginea inferioara a placii pe o lungime de 7,5 cm a liniei de start si se separa prin doua treceri consecutive complete ale sistemului de solventi nifras : cloroform : acetona in raport corespunzator de 5 : 4 : 1, apoi oxidul de aluminiu uscat ce contine sclareol se colecteaza, incepand cu distanta de 2,5 cm de la linia de start si pana la 11,5 cm de la ea, se extrage cu cloroform la 40...45°C consecutiv cu 40, 30, 10 si 10 ml, iar dupa eliminarea cloroformului reziduul obtinut se cantareste si se calculeaza continutul de sclareol. ŕThe invention relates to the chemistry of natural compounds, namely to a process of quantitative determination of sclareol in extracts, obtained from the sage of musk. The essence of the invention consists in that the process includes the preparation of a chromatographic plate with the dimensions 10 x 20 cm, with a layer of aluminum oxide with the first degree of activity having the thickness of 1.8 ... 2.0 mm, after which the sample weighed by The extract dissolves in chloroform, is applied on the plate at a distance of 1.5 cm from the lower edge of the plate on a length of 7.5 cm from the starting line and is separated by two consecutive complete passes of the nifras solvent system: chloroform : acetone with an appropriate ratio of 5: 4: 1, then the dry aluminum oxide containing sclareol is collected, starting with a distance of 2.5 cm from the starting line and up to 11.5 cm from it, it is extracted with chloroform at 40 ... 45 ° C consecutive with 40, 30, 10 and 10 ml, and after the elimination of chloroform the obtained residue is weighed and the sclareol content is calculated. à
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MDA20040036A MD2823G2 (en) | 2004-02-12 | 2004-02-12 | Process for quantitatively determining the sclareol in the obtained from clary sage |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MDA20040036A MD2823G2 (en) | 2004-02-12 | 2004-02-12 | Process for quantitatively determining the sclareol in the obtained from clary sage |
Publications (2)
Publication Number | Publication Date |
---|---|
MD2823F1 true MD2823F1 (en) | 2005-07-30 |
MD2823G2 MD2823G2 (en) | 2006-05-31 |
Family
ID=34859151
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MDA20040036A MD2823G2 (en) | 2004-02-12 | 2004-02-12 | Process for quantitatively determining the sclareol in the obtained from clary sage |
Country Status (1)
Country | Link |
---|---|
MD (1) | MD2823G2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104569215A (en) * | 2015-01-28 | 2015-04-29 | 无锡市人民医院 | Method for determining concentration of sclareol in blood plasma |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU807787A1 (en) * | 1979-01-30 | 1995-11-27 | В.Г. Березкин | Method of qualitative and quantitative determination substances in plane chromatography |
RU2082168C1 (en) * | 1994-01-26 | 1997-06-20 | Сибирский ботанический сад при Томском государственном университете | Method of detection and quantitative determination of ecdisteroids in plant objects |
GB9812413D0 (en) * | 1998-06-10 | 1998-08-05 | Glaxo Group Ltd | Compound and its use |
MD1772G2 (en) * | 1999-09-30 | 2002-04-30 | Государственный Университет Молд0 | Method of sunflower mitochondrions fraction identification |
RU2216018C2 (en) * | 2000-01-17 | 2003-11-10 | Литвинова Лариса Степановна | Method to realize flow thin-layer chromatography and device for its implementation |
-
2004
- 2004-02-12 MD MDA20040036A patent/MD2823G2/en not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104569215A (en) * | 2015-01-28 | 2015-04-29 | 无锡市人民医院 | Method for determining concentration of sclareol in blood plasma |
Also Published As
Publication number | Publication date |
---|---|
MD2823G2 (en) | 2006-05-31 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG4A | Patent for invention issued | ||
KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
MM4A | Patent for invention definitely lapsed due to non-payment of fees |