CN104212886B - A kind of method and system that the unknown source individuality is carried out non-, Europe, East Asia population genetic main constituent analysis - Google Patents

A kind of method and system that the unknown source individuality is carried out non-, Europe, East Asia population genetic main constituent analysis Download PDF

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CN104212886B
CN104212886B CN201410361064.4A CN201410361064A CN104212886B CN 104212886 B CN104212886 B CN 104212886B CN 201410361064 A CN201410361064 A CN 201410361064A CN 104212886 B CN104212886 B CN 104212886B
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李彩霞
魏以梁
叶健
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Institute of Forensic Science Ministry of Public Security PRC
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Abstract

The present invention provides a kind of method and system that the unknown source individuality carries out non-, Europe, East Asia population genetic main constituent analysis, and described method includes extracting the DNA that unknown source is individual；Obtain that 27 of described DNA are non-, Europe, East Asia colony specific position genotype；The genotype of described each specific position utilize STRUCTURE software obtain population genetic principal component analysis result。Scheme provided by the invention can realize individual non-, Europe, the unknown source, East Asia population genetic main constituent analysis, and originates for the unknown that individuality carries out non-, Europe, East Asia colony source is inferred provides data support。

Description

A kind of method and system that the unknown source individuality is carried out non-, Europe, East Asia population genetic main constituent analysis
Technical field
The present invention relates to a kind of method and system inferring that unknown individual is originated, particularly relate to a kind of method and system that the unknown source individuality is carried out non-, Europe, East Asia population genetic main constituent analysis。
Background technology
With economic globalization, country variant, interregional flow of personnel strengthen, and the complicated case such as concerning foreign affairs, anti-terrorism, transregional flow crime is on the increase, and the difficulty of case investigation strengthens day by day。Such as, often there is foreign case in the big city such as Beijing, Shanghai, Guangdong。At present international ethnic group is broadly divided into Europe, Africa, the big ethnic group in East Asia three and the mixing ethnic group formed by these ethnic groups, and the domestic mixing crowd of China mainly includes that the Uygur nationality in Xinjiang, Kazak, the Kirgiz etc. are European and the mixing crowd in East Asia。
Generally, the DNA extracted from criminal-scene biological material can carry out STR detection, and genotyping result input database comparison is searched, or directly compares with the STR typing of target suspect。And in comparison not, we can also utilize the ancestors' information (such as population genetic main constituent analysis) contained in DNA to speculate the colony source of donor, the detection of case can be played directiveness effect by this。In genomic DNA, can there is bigger frequency distribution difference, value of delta in some Autosomal SNPs allele in different crowd > 0.3, this moiety site is defined as AIMs。
Classifying and dividing after deliberation comparatively deep of, Europe non-for intercontinental aspect, this 3 big main crowd of East Asia, defines much different AIMs Sites Combination, and constructs several set composite amplification detection system for practice test in the world。At present, the number of loci comprised due to existing AIMs Sites Combination is more, is not easy to build composite amplification detection system, and owing to being subject to the restriction of SNP detection platform, these detection system having been built up also are difficult to promote the use of。
Therefore, how to provide a kind of method and system that can effectively the unknown source individuality be carried out non-, Europe, East Asia population genetic main constituent analysis, and there is wide applicability become and have problem to be solved。
Summary of the invention
The invention provides a kind of method that the unknown source individuality is carried out non-, Europe, East Asia population genetic main constituent analysis, 27 by obtaining the individual DNA in unknown source non-, Europe, East Asia colony specific position genotype, in conjunction with STRUCTURE software to individual non-, Europe, the unknown source, East Asia population genetic main constituent analysis, thus for carrying out individual non-, Europe, unknown source, East Asia colony source is inferred provides data support。
Present invention also offers a kind of system that the unknown source individuality is carried out non-, Europe, East Asia population genetic main constituent analysis, utilize the composite amplification detection system in this system quickly to obtain individual DNA27 in unknown source be non-, Europe, East Asia colony specific position genotype, and then be combined with existing software, it is achieved to individual non-, Europe, the unknown source, East Asia population genetic main constituent analysis。
Present invention also offers a kind of composite amplification detection system, can quickly obtain that individual DNA27 in unknown source be non-, Europe, East Asia colony specific position genotype, for obtaining individual non-, Europe, unknown source, East Asia population genetic principal component analysis result provides data support。
Present invention also offers a kind of detection kit, containing described composite amplification detection system。This test kit can quickly obtain that individual DNA27 in unknown source be non-, Europe, East Asia colony specific position genotype。
A kind of method that the unknown source individuality is carried out non-, Europe, East Asia population genetic main constituent analysis provided by the invention, the method includes:
1) DNA that the unknown source is individual is extracted；
2) 27 that obtain described DNA non-, Europe, the genotype of East Asia colony specific position, described 27 non-, Europe, East Asia colony specific position is: rs595961, rs2710684, rs260690, rs10496971, rs10497191, rs6444724, rs1586861, rs28777, rs13182883, rs1366220, rs159606, rs7752055, rs321198, rs366178, rs3780962, rs4244304, rs1498553, rs3825663, rs728404, rs1448485, rs7170869, rs1453858, rs2342747, rs4787040, rs881929, rs1197062 and rs4789193；
3) genotype of described each specific position utilize STRUCTURE software obtain population genetic principal component analysis result。
Further, above-mentioned 2) include adopting with described 27 specific position 27 pairs of amplimers one to one its step expanding to obtain amplified production；And amplified production described in use and described 27 specific position 27 extension primer pairs one to one carries out extending to obtain the step of extension products。
Further, described amplimer is the nucleotide sequence of SEQ ID No .1 to SEQIDNo.54；Described extension primer is the nucleotide sequence of SEQ ID No .55 to SEQIDNo.81。
In a specific embodiment of the present invention, 2), after being additionally included in acquisition extension products, genetic analyzer is used to analyze this extension products, to obtain the genotypic step of described 27 specific position。In the solution of the present invention, described genetic analyzer can be the conventional use of genetic analyzers of those skilled in the art, for instance ABI3130 or ABI3500 type genetic analyzer, passes throughThe genotype of 27 specific position described in this extension products analyzed by ID-X software or other GeneMapper software etc.。
A kind of system that the unknown source individuality carries out non-, Europe, East Asia population genetic main constituent analysis provided by the invention, including DNA extraction system, composite amplification detection system, data acquisition system；
The DNA that described DNA extraction system is individual for extracting unknown source；
Described composite amplification detection system is non-for 27 that obtain described DNA, Europe, the genotype of East Asia colony specific position, described 27 non-, Europe, East Asia colony specific position is: rs595961, rs2710684, rs260690, rs10496971, rs10497191, rs6444724, rs1586861, rs28777, rs13182883, rs1366220, rs159606, rs7752055, rs321198, rs366178, rs3780962, rs4244304, rs1498553, rs3825663, rs728404, rs1448485, rs7170869, rs1453858, rs2342747, rs4787040, rs881929, rs1197062 and rs4789193；
Described data acquisition system is for being utilized STRUCTURE software to obtain population genetic principal component analysis result by the genotype of described each specific position。
In the scheme of the application, STRUCTURE software is the existing data analysis software in this area, and its using method is known in the art, and utilizes above-mentioned software to implement the solution of the present invention and can realize for a person skilled in the art。
Further, it is expanded to obtain amplified production with described 27 specific position 27 pairs of amplimers one to one by described composite amplification detection system for adopting, and use and described 27 specific position 27 extension these amplified productions of primer pair one to one carry out extending to obtain extension products, and obtained the genotype of 27 specific position of the individual DNA in unknown source by the extension products obtained。
Further, described amplimer is the nucleotide sequence of SEQ ID No .1 to SEQIDNo.54, and described extension primer is the nucleotide sequence of SEQ ID No .55 to SEQIDNo.81。
A kind of composite amplification detection system provided by the invention, including the individual DNA in unknown source, 27 non-, Europe, East Asia colony specific position, amplimer and extend primer,
Described composite amplification detection system obtains amplified production for utilizing 27 specific position of the DNA of amplimer amplification the unknown source individuality, and use extension this amplified production of primer pair to extend, and obtained the genotype of 27 specific position of the individual DNA in unknown source by the extension products obtained；
Described 27 non-, Europe, East Asia colony specific position are: rs595961, rs2710684, rs260690, rs10496971, rs10497191, rs6444724, rs1586861, rs28777, rs13182883, rs1366220, rs159606, rs7752055, rs321198, rs366178, rs3780962, rs4244304, rs1498553, rs3825663, rs728404, rs1448485, rs7170869, rs1453858, rs2342747, rs4787040, rs881929, rs1197062 and rs4789193；
Described amplimer is formed by with described 27 specific position 27 pairs of amplimers one to one, and described amplimer is the nucleotide sequence of SEQ ID No .1 to SEQIDNo.54；
Described extension primer is formed by with described 27 specific position 27 extension primers one to one, and described extension primer is the nucleotide sequence of SEQ ID No .55 to SEQIDNo.81。
A kind of detection kit provided by the invention, it is characterised in that include described composite amplification detection system。
In the solution of the present invention, the present invention utilizes described composite amplification detection system to carry out the method for typing of 27 specific position, including: 1) using the individual DNA in unknown source that extracts as template；2) use described amplimer that the individual DNA in unknown source as template is carried out multiplexed PCR amplification to react to obtain amplified production；3) extension this amplified production of primer pair is used to carry out extending (such as SNaPshot extends)；4) genetic analyzer is utilized to be analyzed described extension products, to obtain the genotyping result of 27 specific position。
The combination of above-mentioned 27 specific position is that applicant passes through to consult a large amount of list of references, by non-, Europe, the living environment of East Asia crowd, ethnic origin etc. are comprehensively analyzed, investigate the phenotypic characteristic difference of each department nationality population, including resemblance, physical signs etc., document and network data base investigation is carried out, the combination in the site that can carry out Africa, Europe, the deduction of East Asia individuality source obtained on the basis of existing research for these differences。And the combination in these sites is suitable for building one-tube composite amplification system。
Described unknown source individuality can be from African, Europe, East Asia crowd, and the sample of its mixing crowd, for instance blood sample, exfoliative cyte, bone, tooth, seminal stain and buccal swab equal samples, and the individual source of these samples is unknown。
27 non-for the present invention described, Europe, East Asia colony specific position information as shown in table 1:
Table 1
Sequence number SNP site Chromosome Allele
1 rs595961 1 A/G
2 rs2710684 2 C/T
3 rs260690 2 A/C
4 rs10496971 2 G/T
5 rs10497191 2 C/T
6 rs6444724 3 A/G
7 rs1586861 3 C/T 3 -->
8 rs28777 5 A/C
9 rs13182883 5 A/G
10 rs1366220 5 C/T
11 rs159606 5 A/G
12 rs7752055 6 C/T
13 rs321198 7 A/G
14 rs366178 8 G/T
15 rs3780962 10 A/G
16 rs4244304 10 C/T
17 rs1498553 11 A/G
18 rs3825663 14 C/T
19 rs728404 15 A/G
20 rs1448485 15 A/C
21 rs7170869 15 A/G
22 rs1453858 15 A/T
23 rs2342747 16 A/G
24 rs4787040 16 A/T
25 rs881929 16 G/T
26 rs1197062 17 A/C
27 rs4789193 17 A/T
Preferred amplimer sequence provided by the invention is as follows。27 couples of amplimer PRIMERPREMIER5.0 (PREMIERBiosoft, PaloAlto, CA, USA) design, and described 27 pairs of amplimers and corresponding locus thereof are as shown in table 2 below, and PCRU represents forward primer, and PCRL represents downstream primer；
The solution of the present invention has the advantage that
1, the inventive method and system can carry out Africa, Europe, East Asia crowd, and ancestors' composition of its mixing crowd (such as the Central Asia crowd mixing of East Asia crowd (Europe) etc.) divides (i.e. population genetic principal component analysis), in concerning foreign affairs, anti-terrorism case, biological specimen particularly with source, the not agnate region crowd extracted in above-mentioned case, in STRs typing comparison not, determine colony's identity source of personnel concerning the case, make case fast qualitative and clearly investigate direction, can play a significant role。
2, the present processes and system, adopt the combination of 27 specific position, constitute and the detection system that the unknown source individuality can be carried out effective composite amplification, especially one-tube composite amplification system, DNA profiling amount can be saved, and be applicable to capillary electrophoretic analysis method。This make the present invention method and system can effectively various relating to non-, Europe, extensive use in case that East Asia colony source is inferred。
3, be can be seen that by embodiment data, the present processes and system are applied to the non-of 2 case 4 example DNA donors, Europe, East Asia population genetic main constituent is constituted have been analyzed, analyze result and originate consistent with the known colony of case, further, 422 parts of samples for the known source selected from HapMap data base (include from Africa, Europe, East Asia crowd, and its mixing crowd sample) population genetic main constituent constitute be analyzed, it is same consistent with the known source of these samples that the colony's main constituent drawn by this analysis result constitutes result, illustrate by the present invention method and system obtain non-, Europe, East Asia population genetic main constituent analysis result, can be non-, Europe, the source deduction of East Asia colony provides data support accurately。
Accompanying drawing explanation
.27 SNP site of Fig. 1 adopts STRUCTURE software analysis to obtain 14 crowd STRUCTURE (K=3) colony composition analysis result figure。Wherein, YRI and LWK is as the representative of typical case Africa crowd, and CEU and TSI is as the representative of typical European crowd, and CHB and JPT represents as typical case East Asia crowd。The non-descendants crowd in ASW South West USA area；Northern Europe of CEU Utah, USA and western descendant crowd；CHB Han nationality in Beijing crowd；City of Denver, CHD Colorado Chinese population；The Gu Jilate family of languages India crowd of Houston, GIH Texas；The Japanese population in JPT Tokyo；The Luhya crowd of LWK Kenya Webuye；The Mexican descendant crowd of MEX Los Angeles；The Maasai crowd of MKK Kenya Kinyawa；The Toscani crowd of TSI Italy；The Yoruba crowd of YRI Nigeria Ibadan；The Chinese Han Population of CHN north of China；The Tibetan populations of CTT Tibet, China；The Uygur nationality crowd (Europe and East Asia mixing crowd) of CUX Xinjiang, China。
Fig. 2: in case 1, sample is non-, Europe, East Asia population genetic main constituent pie graph。
Fig. 3: in case 2, sample 1 is non-, Europe, East Asia population genetic main constituent pie graph。
Fig. 4: in case 2, sample 2 is non-, Europe, East Asia population genetic main constituent pie graph。
Fig. 5: in case 2, sample 3 is non-, Europe, East Asia population genetic main constituent pie graph。
Fig. 6 A-6E: to randomly draw from individual African, European, Asian sample adopts the figure that the inventive method and system detection population genetic main constituent are constituted。Fig. 6 A is African sample YRI330, Fig. 6 B is European sample CEU998, Fig. 6 C is people from South Asia (non-, Europe, East Asia mixing) sample GIH1179, Fig. 6 D be gook sample CHN870, Fig. 6 E is people from Central Asia (Europe, East Asia mix) sample CUX1066。
Detailed description of the invention
Embodiment 1 adopt the present invention the unknown source individuality is carried out non-, Europe, the method and system of East Asia population genetic principal component analysis the unknown is originated individuality carries out source and infers
Fig. 1 is that 27 SNP site in the application scheme adopt STRUCTURE software for 14 crowd STRUCTURE (K=3) colony composition analysis result。The result of Fig. 1 indicates this group site can effectively distinguish Africa, Europe, the big crowd in East Asia three and mixing crowd's heredity main constituent thereof。
Individuality in case 1 and case 2 is carried out source and infers by the described method and system adopting the present invention。The skeletal remains that case 1 finds in Northeast China, (in case 1, Bone broken has transferred American side, and identity is it has been acknowledged that be the USAF pilot fallen before 56 years, and European originates)；
In case 2,3 tools burn corpse, need to identify its national source, with the investigation direction (in case 2, the identity of 3 the dead is it has been confirmed that be all from Southern Xinjiang, the Uighurs (i.e. Europe-mixing crowd source, East Asia)) of clear and definite case。
1, the DNA of individuality to be detected is extracted as template
Case 1:1 part Bone broken, uses organic method to extract DNA solution。
Case 2:3 part venous blood (0.5ml), uses DNAbloodmidi test kit (QIAGEN, Hilden, Germany) to extract DNA solution。
2, the DNA profiling extracted is carried out multiplexed PCR amplification reaction
2.1, primer pond configuration
The configuration in amplimer pond, is described 27 SNP site, 27 pairs of amplimers one to one in wherein said amplimer group, and every pair of amplimer can expand and include its corresponding SNP site on DNA to be detected；In the present embodiment, it is preferred that the nucleotide sequence that amplimer group is SEQ ID No .1 to SEQIDNo.54 of described 27 SNP site；Various primer sequence provided by the invention is synthesized by Shanghai Sangon Biological Engineering Technology And Service Co., Ltd。
2.2, multi-PRC reaction
The present embodiment uses the AB9700 type DNA cloning instrument with 96 hole PCR plate to carry out multi-PRC reaction。
(1) configuration PCRmix (27-plexPCR (5 μ L reaction system))
Reagent name Configuration amount (μ L) Concentration
PCR primer pond (27-plex) 0.135 Every primer 0.05 μ Μ
dNTP(10mM) 0.05 0.1mM
(buffer, containing 15mM Mg for 10 × PCR buffer2+) 0.5 Mg2+1.5mM
MgCl2(25mM Mg2+) 0.7 Mg2+3.5mM
Hotstar Taq plus(5U/μL) 0.1 0.1U
ddH2O (deionized water) 1.515
DNA 2 2-10ng/μL
Cumulative volume 5(μL)
It is respectively configured 27 weight PCRmix in the ratio in upper table, after the PCRmix of amplification system is mixed, take in the 5 μ L reacting hole joining 96 hole PCR plate, then in the PCRmix of 3 μ L of above-mentioned reacting hole, add 2 DNA profilings (5 μ L reaction system) to be detected for μ L respectively。After sealer, centrifugal 1 minute of 3000rcf。
(2) amplification program
2.3, PCR primer purification, adds 3 μ L purified reagent
(1) according to the system of following table, Clean-up reagent is prepared
Reagent name Configuration amount (μ L) Concentration
ExoⅠ(10U/μL) 0.2 2U
SAP(1U/μL) 1 1U
10 × SAP buffer 0.3 1×
ddH2O 1.5
Amount to 3
(2) reacted for PCR 96 hole PCR plate 3000rcf are centrifuged 1 minute, add the Clean-up reagent prepared, every hole 3 μ L (every hole system at end is 8 μ L)。After sealer, centrifugal 1 minute of 3000rcf, runs the program shown in following table。
Step Temperature Time
1 37℃ 30min
2 96℃ 10min
3 4℃ ∞
Note: the PCR primer that purification is good can be deposited under-20 DEG C of conditions。
3, the DNA cloning product after purification is carried out SNaPshot extension
(1) 96 hole PCR plate (containing in steps 2 DNA product expanded as extending template) primer extension reaction mixture of system preparation 27-plex shown in following table that defrosting purification is good。
27-plex extension (10 μ L reaction system)
(2) 96 hole PCR plate 3000rcf after defrosting are centrifuged 1 minute, the 7 μ L 27-plex primer extension reaction mixture (every hole system at end is 15 μ L) prepared is added in each hole, sealer, centrifugal 1 minute of 3000rcf, runs the program shown in following table。
Note: primer extension reaction terminate after DNA product can deposit under-20 DEG C of conditions。
(3) extension products purification (removing ddNTPs)
Extension products adds 1USAP (1U/ μ L, 1 μ L), carries out following purification reaction program:
Step Temperature Time
1 37℃ 60min
2 75℃ 15min
3 4℃ ∞
(4) capillary electrophoresis typing
Extension products after purification, in the upper detection of 3500XL genetic analyzer (AppliedBiosystems), uses GeneMapperIDX software (AppliedBiosystems company) analytical data。
4, genotyping result
The genotype of described each specific position utilize STRUCTURE software obtain population genetic principal component analysis result。Result is as shown in Figure 2-5。
Fig. 2 is that in case 1 sample is non-, Europe, East Asia population genetic main constituent pie graph。As seen from Figure 2, African's composition accounts for 0.2%, and European's composition is 99.2%, and gook's composition accounts for 0.6%；This population genetic principal component analysis result supports that DNA donor is originated for European。
Fig. 3 is that in case 2 sample 1 is non-, Europe, East Asia population genetic main constituent pie graph。As seen from Figure 3, African's composition accounts for 0.4%, and European's composition is 38.9%, and gook's composition accounts for 60.7%；This population genetic principal component analysis result supports that DNA donor is Europe-mixing crowd source, East Asia。
Fig. 4 is that in case 2 sample 2 is non-, Europe, East Asia population genetic main constituent pie graph。As seen from Figure 4, African's composition accounts for 2.3%, and European's composition is 51.1%, and gook's composition accounts for 46.6%；This population genetic principal component analysis result supports that DNA donor is Europe-mixing crowd source, East Asia。
Fig. 5 is that sample 3 is non-, Europe, East Asia population genetic main constituent pie graph。As seen from Figure 5, African's composition accounts for 2.6%, and European's composition is 43.0%, and gook's composition accounts for 54.4%；This population genetic principal component analysis result supports that DNA donor is Europe-mixing crowd source, East Asia。
Non-, Europe of the above-mentioned sample obtained by the present embodiment method, East Asia population genetic principal component analysis result, analyze result and the known colony of case sample source consistent, illustrates the population genetic principal component analysis result obtained by the inventive method and system can for carrying out non-, Europe, East Asia colony originates deduction offer data support。
Embodiment 2
Utilize the method and system that embodiment 1 is identical, adopt identical step to above-mentioned sample to randomly draw from individual African, European, Asian sample adopts the inventive method and system detection population genetic main constituent component analysis。
The sample extracted is following 5 parts: Fig. 6 A is African sample YRI330, Fig. 6 B is European sample CEU998, Fig. 6 C is people from South Asia (non-, Europe, East Asia mixing) sample GIH1179, Fig. 6 D is gook sample CHN870, Fig. 6 E is people from Central Asia (Europe, East Asia mixing) sample CUX1066。As shown in Figure 6, pie chart is individual non-, Europe, East Asia population genetic main constituent composition to result。Individual possible crowd source is represented from big to small, it can be seen that the analysis result of population genetic main constituent is originated consistent with the known colony of sample by numerical value。
Embodiment 3
The 422 parts of samples utilizing the method and system of the present invention known source to selecting in HapMap data base re-start ethnic origin and infer, check the accuracy of above-mentioned non-, Europe, East Asia and its mixing ethnic group inference system, and result is as shown in table 4:
Table 4 is non-, Europe, the East Asia ethnic group inference system 422 parts of sample inferred results to selecting
Wherein, 422 parts of samples are it is known that African's sample (sample size 84 people), European's sample (sample size 85 people), gook's sample (sample size 86 people), people from Central Asia (Europe, East Asia mixing) sample (sample size 165 people)
By the method and system of the present invention, above-mentioned 422 parts of samples are carried out principal component analysis, African's sample all can be inferred as African, European's sample is all inferred as European, gook's sample is all inferred as gook, the result of people from Central Asia is the blending constituent in Europe, East Asia, and all sample standard deviations meet the ethnic origin that it is known。Therefore by said system can for carrying out non-, Europe, the source deduction of East Asia colony provides data support accurately。

Claims (5)

1. the method that the unknown source individuality is carried out non-, Europe, East Asia population genetic main constituent analysis, it is characterised in that the method includes:
1) DNA that the unknown source is individual is extracted；
2) 27 that obtain described DNA non-, Europe, the genotype of East Asia colony specific position, described 27 non-, Europe, East Asia colony specific position is: rs595961, rs2710684, rs260690, rs10496971, rs10497191, rs6444724, rs1586861, rs28777, rs13182883, rs1366220, rs159606, rs7752055, rs321198, rs366178, rs3780962, rs4244304, rs1498553, rs3825663, rs728404, rs1448485, rs7170869, rs1453858, rs2342747, rs4787040, rs881929, rs1197062 and rs4789193；
Also include adopting with described 27 specific position 27 pairs of amplimers one to one its step expanding to obtain amplified production；And amplified production described in use and described 27 specific position 27 extension primer pairs one to one carries out extending to obtain the step of extension products；
Described amplimer is the nucleotide sequence of SEQ ID No .1 to SEQIDNo.54；Described extension primer is the nucleotide sequence of SEQ ID No .55 to SEQIDNo.81；
3) genotype of described each specific position utilize STRUCTURE software obtain population genetic principal component analysis result。
2. method according to claim 1, it is characterised in that wherein 2) be additionally included in acquisition extension products after, use genetic analyzer to analyze this extension products, to obtain the genotypic step of described 27 specific position。
3. the system that the unknown source individuality is carried out non-, Europe, East Asia population genetic main constituent analysis, it is characterised in that described system includes DNA extraction system, composite amplification detection system, data acquisition system；
The DNA that described DNA extraction system is individual for extracting unknown source；
Described composite amplification detection system is non-for 27 that obtain described DNA, Europe, the genotype of East Asia colony specific position, described 27 non-, Europe, East Asia colony specific position is: rs595961, rs2710684, rs260690, rs10496971, rs10497191, rs6444724, rs1586861, rs28777, rs13182883, rs1366220, rs159606, rs7752055, rs321198, rs366178, rs3780962, rs4244304, rs1498553, rs3825663, rs728404, rs1448485, rs7170869, rs1453858, rs2342747, rs4787040, rs881929, rs1197062 and rs4789193；
Wherein, it is expanded to obtain amplified production with described 27 specific position 27 pairs of amplimers one to one by described composite amplification detection system for adopting, and use and described 27 specific position 27 extension these amplified productions of primer pair one to one carry out extending to obtain extension products, and obtained the genotype of 27 specific position of the individual DNA in unknown source by the extension products obtained；
Described amplimer is the nucleotide sequence of SEQ ID No .1 to SEQIDNo.54, and described extension primer is the nucleotide sequence of SEQ ID No .55 to SEQIDNo.81；
Described data acquisition system is for being utilized STRUCTURE software to obtain population genetic principal component analysis result by the genotype of described each specific position。
4. a composite amplification detection system, it is characterised in that described system includes the individual DNA in unknown source, 27 non-, Europe, East Asia colony specific position, amplimer and extend primer,
Described composite amplification detection system obtains amplified production for utilizing 27 specific position of the DNA of amplimer amplification the unknown source individuality, and use extension this amplified production of primer pair to extend, and obtained the genotype of 27 specific position of the individual DNA in unknown source by the extension products obtained；
Described 27 non-, Europe, East Asia colony specific position are: rs595961, rs2710684, rs260690, rs10496971, rs10497191, rs6444724, rs1586861, rs28777, rs13182883, rs1366220, rs159606, rs7752055, rs321198, rs366178, rs3780962, rs4244304, rs1498553, rs3825663, rs728404, rs1448485, rs7170869, rs1453858, rs2342747, rs4787040, rs881929, rs1197062 and rs4789193；
Described amplimer is formed by with described 27 specific position 27 pairs of amplimers one to one, and described amplimer is the nucleotide sequence of SEQ ID No .1 to SEQIDNo.54；
Described extension primer is formed by with described 27 specific position 27 extension primers one to one, and described extension primer is the nucleotide sequence of SEQ ID No .55 to SEQIDNo.81。
5. a detection kit, it is characterised in that include the composite amplification detection system described in claim 4。
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