CN107400713B - Method and system for identifying individuals of Tibetan nationality of Tibet plateau in 27 groups - Google Patents
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Abstract
The invention provides a method and a system for identifying individuals of Tibetan nationality of Tibet plateau in 27 populations, wherein the method comprises the steps of extracting DNA of the individuals; obtaining the genotypes of 87 Tibetan specific plateau adaptive SNP loci of the DNA; and (3) obtaining the group genetic component analysis result of the individual by using STRUCTURE software according to the genotype of each specific site, and identifying whether the individual is the group individual of the Tibetan nationality of the Tibet plateau of China according to the result. The scheme provided by the invention can identify the individual as the individual of the Tibetan nationality of the Tibet plateau or the individual of the Tibetan nationality of the non-Tibet plateau in 27 groups. The scheme of the invention can increase the resolution ratio aiming at the east Asian population and can provide a detection clue for related cases.
Description
Technical Field
The invention relates to a method and a system for identifying individual group sources, in particular to a method and a system for identifying individuals of Tibetan nationality of the Tibet plateau in 27 groups.
Background
Along with the economic globalization, the personnel flow among different countries and regions is increased, the complicated cases concerning foreign matters, counterterrorism, cross-region flow crime and the like are continuously increased, and the difficulty of case investigation is increasingly increased. For example, foreign-related cases often occur in large cities such as Beijing, Shanghai, Guangdong, etc. Currently, the international races are mainly classified into african, american, european, south asia, east asia races and mixed races formed by these races.
In general, ancestral information (e.g., population genetic component analysis) contained in DNA extracted from field biological samples of a case is used to infer the source of a donor population, which can be instructive in case detection. SNP loci with obvious allele frequency difference in different populations can be used as AISNPs, and a group of AISNPs loci are used for analyzing genetic component composition of different populations so as to realize population differentiation.
The Tibetan nationality of the Qinghai-Tibet plateau of China, as the east Asian population, stays in the Qinghai-Tibet plateau with the elevation of 3000-5000 meters for a long time, forms a unique plateau adaptation mechanism through long-term natural selection, the mechanism is related to genes EGLN1, EPAS1 and the like, and a large number of Single Nucleotide Polymorphism Sites (SNPs) exist on the genes. Yangxin et al carried out genetic polymorphism analysis on 10 SNPs sites on EPAS1 and EGLN1 genes, and found that 4 SNPs sites (rs150877473, rs1562453, rs186996510 and rs7589621) have significant differences among Tibetan-Han people. However, there is currently no effective method available to identify individuals from the Tibetan nationality of the Tibet plateau of China among the African, American, European, south Asia and east Asia populations.
How to provide an effective method or system for identifying whether an individual is an individual of the Tibetan in Qinghai-Tibet plateau in Africa, American, European, south Asia and east Asia population becomes a problem to be solved.
Disclosure of Invention
The invention provides a method for identifying individuals of Tibetan nationality of Qinghai-Tibet plateau in 27 populations, which comprises the steps of obtaining genotypes of 87 Tibetan nationality specific plateau adaptive SNP loci of DNA of the individuals, obtaining a population genetic component analysis result of the individuals by combining STRUCTURE software, and identifying whether the individuals are the Tibetan nationality individuals of the Qinghai-Tibet plateau according to the result.
The invention also provides a system for identifying the individuals of the Tibetan nationality of the Tibet plateau in 27 populations, the DNA 87 Tibetan specific plateaus of the individuals can be quickly obtained by utilizing a composite amplification detection system in the system to adapt to the genotype of the SNP locus, and then the genotype is combined with the existing software to obtain the population genetic component analysis result of the individuals, and whether the individuals are the individuals of the Tibetan nationality of the Tibet plateau is identified according to the result.
The invention also provides a composite amplification detection system which can rapidly obtain genotypes of 87 Tibetan specific plateau adaptive SNP sites of the DNA of an individual and provide data support for obtaining a genetic component analysis result of the individual.
The invention also provides a detection kit containing the composite amplification detection system. The kit can rapidly obtain genotypes of 87 Tibetan specific plateau adaptive SNP loci of individual DNA.
The invention provides a method for identifying individuals of Tibetan nationality of Tibet plateau in 27 populations, which comprises the following steps:
1) extracting DNA of an individual;
2) obtaining genotypes of 87 Tibetan specific plateau adaptive SNP sites of the DNA, wherein the 87 Tibetan specific plateau adaptive SNP sites are as follows: rs10178633, rs10193827, rs10206434, rs1109285, rs1109286, rs11122280, rs11125068, rs115321619, s116062164, rs116611511, rs11675232, rs11675441, rs11689011, rs11805033, rs12467821, rs13003074, rs13006131, rs13419896, rs13424253, rs1374749, rs141366568, rs141426873, rs 1428201, rs1447563, rs 149391 391, rs 1507473, rs 15624242424563, rs 170950, rs 35010, rs 35017017013, rs1867784, rs 8092, rs1992846, rs2066140, rs2121266, rs2153364, rs 22449279, rs2275279, rs2486729, rs 8686241867356355635563556355639, rs 755635563556355635563556355635563556355648, rs 75563556355635563556355635563556355635563556355648, rs 7556355635563556355635563556355635563556355648, rs 75563556355635563556355635563556355648, rs 75563556355648, rs 755635563556355635563556355648, rs 755648, rs 7556375637563756375648, rs 75563756375637563756375637563756375637563756375648, rs 7556375637563756375648, rs 7556375637563756375637563756375637563756375637563756375648, rs 75563756375637563756375648, rs 755637563756375637563756375637563756375637563756375637563756;
3) obtaining the group genetic component analysis result of the individual by using STRUCTURE software for the genotype of each specific site, and identifying whether the individual is a group individual of the Tibetan nationality of the Tibet plateau of China according to the result;
the 27 populations are the western population of Omobia, the Mende population of Seralane, the Jolaba population of Ileba of Nigeria, the West population of Kenya Luysia, the Eisen population of Nigeria, the Black population of American, the Balados island population of Caribbean, the Peru Lema population, the Columbia population of Golomis Mderlin, the populations of Podochiza, the Mexico population of los Angeles, the northern Europe and Western Africa of Utah, the Finland population of Finland, the British population of Scotland and England, the Ibiya population of Spanish, the Talania population of Italy, the Mengla population of Menglatiria, the Pakistan population of Beijing, the North Kyokura population of Texas Houston, the Indian Han Guugen population, the Smith population of Milan, the Japanese Pigeon, the Beijing Hodgkin of Beijing of Texas, Chinese southern Han people, Vietnam Jing people, Chinese Xishuangbanna Dai people and Chinese Qinghai-Tibet plateau Tibetan people.
Further, the 2) above includes the steps of using 87 pairs of amplification primers corresponding to the 87 specificity sites one by one to amplify the primer pair to obtain amplification products; and a step of extending the amplification product by using 87 extension primers corresponding to the 87 specificity sites one by one to obtain an extension product.
Furthermore, the amplification primer is a nucleotide sequence from SEQ ID No.1 to SEQ ID No.174 in the sequence table; the extension primer is a nucleotide sequence from SEQ ID No.175 to SEQ ID No.261 in the sequence table.
In one embodiment of the present invention, 2) further comprises the step of analyzing the extension product using a genotyping system after obtaining the extension product to obtain the genotypes of the 87 specific sites. In the embodiment of the present invention, the genotyping system may be a genetic analyzer conventionally used by those skilled in the art, such as ABI3130 or ABI3500 type genetic typingAn analyzer, or
The genotyping system (Agena, USA) obtains the genotypes of the 87 specific sites of the extension product.
The invention provides a system for identifying individuals of Tibetan nationality of Tibet plateau in 27 populations, which comprises a DNA extraction system, a composite amplification detection system and a data acquisition system;
the DNA extraction system is used for extracting DNA of individuals;
the composite amplification detection system is used for obtaining genotypes of 87 Tibetan specific plateau adaptive SNP sites of the DNA, wherein the 87 Tibetan specific plateau adaptive SNP sites are as follows: rs10178633, rs10193827, rs10206434, rs1109285, rs1109286, rs11122280, rs11125068, rs115321619, s116062164, rs116611511, rs11675232, rs11675441, rs11689011, rs11805033, rs12467821, rs13003074, rs13006131, rs13419896, rs13424253, rs1374749, rs141366568, rs141426873, rs 1428201, rs1447563, rs 149391 391, rs 1507473, rs 15624242424563, rs 170950, rs 35010, rs 35017017013, rs1867784, rs 8092, rs1992846, rs2066140, rs2121266, rs2153364, rs 22449279, rs2275279, rs2486729, rs 8686241867356355635563556355639, rs 755635563556355635563556355635563556355648, rs 75563556355635563556355635563556355635563556355648, rs 7556355635563556355635563556355635563556355648, rs 75563556355635563556355635563556355648, rs 75563556355648, rs 755635563556355635563556355648, rs 755648, rs 7556375637563756375648, rs 75563756375637563756375637563756375637563756375648, rs 7556375637563756375648, rs 7556375637563756375637563756375637563756375637563756375648, rs 75563756375637563756375648, rs 755637563756375637563756375637563756375637563756375637563756;
the data acquisition system is used for acquiring a group genetic component analysis result of the individual by using STRUCTURE software according to the genotype of each specific site, and identifying whether the individual is a group individual of the Tibetan in Qinghai-Tibet plateau;
the 27 populations are the western population of Omobia, the Mende population of Seralane, the Jolaba population of Ileba of Nigeria, the West population of Kenya Luysia, the Eisen population of Nigeria, the Black population of American, the Balados island population of Caribbean, the Peru Lema population, the Columbia population of Golomis Mderlin, the populations of Podochiza, the Mexico population of los Angeles, the northern Europe and Western Africa of Utah, the Finland population of Finland, the British population of Scotland and England, the Ibiya population of Spanish, the Talania population of Italy, the Mengla population of Menglatiria, the Pakistan population of Beijing, the North Kyokura population of Texas Houston, the Indian Han Guugen population, the Smith population of Milan, the Japanese Pigeon, the Beijing Hodgkin of Beijing of Texas, Chinese southern Han people, Vietnam Jing people, Chinese Xishuangbanna Dai people and Chinese Qinghai-Tibet plateau Tibetan people.
In the present embodiment, the stuctrure software is data analysis software existing in the field, and the method of using the same is known in the field, and it is obvious to those skilled in the art that the embodiment of the present invention can be implemented by using the above software.
Further, the multiplex amplification detection system is configured to amplify the 87 pairs of amplification primers corresponding to the 87 specific sites one by one to obtain an amplification product, extend the amplification product using 87 extension primers corresponding to the 87 specific sites one by one to obtain an extension product, and obtain genotypes of the 87 specific sites of the individual DNA from the obtained extension product.
Furthermore, the amplification primer is a nucleotide sequence from SEQ ID No.1 to SEQ ID No.174 in the sequence table, and the extension primer is a nucleotide sequence from SEQ ID No.175 to SEQ ID No.261 in the sequence table.
The invention provides a composite amplification detection system, which comprises individual DNA, 87 Tibetan specific plateau adaptive SNP sites, amplification primers and extension primers,
the composite amplification detection system is used for amplifying 87 specific sites of individual DNA by using an amplification primer to obtain an amplification product, extending the amplification product by using an extension primer, and obtaining the genotype of the 87 specific sites of the individual DNA from the obtained extension product;
the 87 Tibetan specific plateau adaptive SNP loci are as follows: rs10178633, rs10193827, rs10206434, rs1109285, rs1109286, rs11122280, rs11125068, rs115321619, s116062164, rs116611511, rs11675232, rs11675441, rs11689011, rs11805033, rs12467821, rs13003074, rs13006131, rs13419896, rs13424253, rs1374749, rs141366568, rs141426873, rs 1428201, rs1447563, rs 149391 391, rs 1507473, rs 15624242424563, rs 170950, rs 35010, rs 35017017013, rs1867784, rs 8092, rs1992846, rs2066140, rs2121266, rs2153364, rs 22449279, rs2275279, rs2486729, rs 8686241867356355635563556355639, rs 755635563556355635563556355635563556355648, rs 75563556355635563556355635563556355635563556355648, rs 7556355635563556355635563556355635563556355648, rs 75563556355635563556355635563556355648, rs 75563556355648, rs 755635563556355635563556355648, rs 755648, rs 7556375637563756375648, rs 75563756375637563756375637563756375637563756375648, rs 7556375637563756375648, rs 7556375637563756375637563756375637563756375637563756375648, rs 75563756375637563756375648, rs 755637563756375637563756375637563756375637563756375637563756;
the amplification primers consist of 87 pairs of amplification primers which correspond to the 87 specific sites one by one, and the amplification primers are nucleotide sequences from SEQ ID No.1 to SEQ ID No.174 in a sequence table;
the extension primers consist of 87 extension primers which correspond to the 87 specific sites one by one, and the extension primers are nucleotide sequences from SEQ ID No.175 to SEQ ID No.261 in a sequence table; the 27 populations are the western population of Omobia, the Mende population of Seralane, the Jolaba population of Ileba of Nigeria, the West population of Kenya Luysia, the Eisen population of Nigeria, the Black population of American, the Balados island population of Caribbean, the Peru Lema population, the Columbia population of Golomis Mderlin, the populations of Podochiza, the Mexico population of los Angeles, the northern Europe and Western Africa of Utah, the Finland population of Finland, the British population of Scotland and England, the Ibiya population of Spanish, the Talania population of Italy, the Mengla population of Menglatiria, the Pakistan population of Beijing, the North Kyokura population of Texas Houston, the Indian Han Guugen population, the Smith population of Milan, the Japanese Pigeon, the Beijing Hodgkin of Beijing of Texas, Chinese southern Han people, Vietnam Jing people, Chinese Xishuangbanna Dai people and Chinese Qinghai-Tibet plateau Tibetan people.
The invention provides a detection kit which is characterized by comprising the composite amplification detection system.
In the scheme of the invention, the method for typing 87 specific sites by using the composite amplification detection system comprises the following steps: 1) using the extracted individual DNA as a template; 2) performing a multiplex PCR amplification reaction on the individual DNA as a template using the amplification primers to obtain an amplification product; 3) extending the amplification product using an extension primer; 4) the extension products were analyzed using a genotyping system to obtain the results of typing of 87 specific sites.
The 87 specific site combinations are obtained by the applicant on the basis of the existing research through consulting a large number of references, comprehensively analyzing the living environment, ethnic origin and the like of the African, American, European, south Asia and east Asia population, and simultaneously combining the phenotypic characteristic differences of the individuals of the Tibetan population of the Qinghai-Tibet plateau, including the shape characteristics, the physiological indexes and the like, researching documents and network databases aiming at the differences, and identifying whether the individuals are the sites of the individual of the Tibetan population of the Tibetan plateau of the China in the African, American, European, south Asia and east Asia (the 27 populations in the application).
The individuals may be samples from the 27 population of individuals, such as blood samples, exfoliated cells, bone, teeth, seminal plaques, and buccal swabs. The individual can be identified as a population of the Tibetan in China or a population of the Tibetan not in Tibetan in China (namely, an individual of one of the other 26 populations) by the method and the system of the invention.
The information of the 87 Tibetan specific plateaus adaptive to the SNP loci is shown in Table 1:
TABLE 1
The preferred amplification primers provided by the invention, namely 87 pairs of amplification primers and corresponding loci thereof, are shown in table 2, wherein PCRU represents an upstream primer, and PCRL represents a downstream primer;
the scheme of the invention has the following advantages:
1. the scheme provided by the invention can identify the individual as the individual of the Tibetan nationality of the Tibet plateau or the individual of the Tibetan nationality of the non-Tibet plateau in 27 groups. Therefore, the scheme of the invention can increase the resolution ratio aiming at east Asia population, thereby playing an important role in determining the population identity source of the involved personnel and ensuring the rapid qualitative and definite investigation direction of the case.
2. The method and the system adopt the combination of 87 specific sites to construct a detection system which can effectively perform composite amplification on individuals. This makes the method and system of the present invention effective for a wide range of applications in a variety of cases involving inferences about the origin of the African, American, European, south Asia, east Asia population (the 27 populations in this application).
3. It can be seen from the example data that the method and system of the present application were applied to 27 population 2687 individuals for analysis, and the analysis results were consistent with the known population sources of the individuals, indicating that the method and system of the present invention can identify the individuals as individuals of the Tibetan nationality of the Tibet China or individuals of the Tibetan nationality of non-Tibet China among the 27 populations.
Drawings
FIG. 1 is a graph showing the results of analysis of genetic components of 2687 individuals of 27 human groups of STRUCTUREs (K ═ 2) by analyzing 87 SNP sites using STRUCTURE software. Wherein, AFR is Africa; AMR is American; EUR is Europe; EAS, east Asia; SAS is south Asia. Each bar represents an individual, each color represents a genetic component, component 1 is represented in yellow (lighter bar) and component 2 is represented in red (darker bar), and the color composition ratio represents the ratio of the individual genetic components;
FIG. 2 is a diagram showing the analysis results of the genetic components of a population of 27 persons obtained by analyzing 87 SNP sites with STRUCTURE software. Africa; AMR is American; EUR is Europe; EAS, east Asia; SAS is south Asia. Each pie represents a population with component 1 in yellow (lighter pie) and component 2 in red (darker pie), with the color composition ratio representing the ratio of genetic components of that population (or population).
Detailed Description
Example 1 Using the method and system of the present invention to identify individuals among 27 populations as individuals of the Tibetan nationality of the Tibet plateau, China, or individuals of the Tibetan nationality of the Tibet plateau, or not
The sample information of 27 populations of the present invention is shown in table 4 below, for a total of 2687 samples, where: (1) 183 venous blood sample biological samples of the Tibetan group are derived from a national scientific and technological resource sharing service platform plan project (number: YCZYPT [2017]01-3) and a capital project (number: 2017JB025) special for the basic scientific research business expense of a material evidence identification center of the ministry of public Security, are collected from Tibetan places of Qinghai-Tibet plateau, and are signed with an informed consent and have definite ancestral information; (2) typing data for 2504 samples of 26 world populations were obtained from the thousand human genome database.
TABLE 4
The following will illustrate the implementation process of the method of the present invention by taking a sample of a randomly extracted individual of Tibetan of Tibet plateau and a randomly extracted individual of Tibetan of non-Tibetan of Tibet plateau (i.e. one of the other 26 populations) as examples.
1. Extracting DNA of individual to be detected as template
Sample 1: venous blood of Tibetan nationality of Qinghai-Tibet plateau in China, and its application
DNA was extracted using DNA blood midi kit (Qiagen, Germany).
Sample 2: venous blood of non-Chinese Qinghai-Tibet plateau Tibetan group individual, and its application
DNA was extracted using a DNA bloodmidi kit (Qiagen, Germany).
2. SNP typing of extracted DNA template
The study uses
Genotyping assays were performed using a genotyping system (Agena, USA).
The basic principle is as follows:
combining with a micro sequencing technology and a MALDI-TOF-MS technology, a sample to be detected can be adsorbed on a matrix of a chip and forms crystals, then charge transfer is generated through laser irradiation in a mass spectrometer, the ionized sample reaches a detector through a vacuum tube in a flying way under the action of an electric field, the shorter the DNA fragment is, the earlier the DNA fragment reaches the detector, and the mass spectrometer can distinguish different DNA fragments with a base difference.
The method comprises the following basic steps:
primer design
Multiplex PCR amplification
SAP purification
Single base extension
Resin purification
Spotting to a chip
MALDI-TOF (matrix assisted laser Desorption ionization-time of flight) mass spectrometry detection
(1) Designing a primer: amplification and extension primer Design were performed on 87 SNP sites (shown in Table 1) using Assay Design Suite v2.0 software (Agena, USA) (specific primers are shown in tables 2 and 3). The primers were synthesized by Biotechnology engineering (Shanghai) Inc. 87 sites are divided into 3 groups (28-plex, 34-plex and 25-plex) to prepare a PCR primer pool and an extension primer pool respectively; the specific ratios are shown in Table 5(28-plex primer ratio Table), Table 6(34-plex primer ratio Table), and Table 7(25-plex primer ratio Table).
TABLE 5
Note: EX denotes an extension primer
TABLE 6
TABLE 7
(2) PCR composite amplification: preparing PCR composite amplification mixed solution according to a PCR mixed solution proportioning table 8, adding the PCR composite amplification mixed solution into reaction holes of a 96-hole PCR plate, sealing a membrane, vibrating, performing instant centrifugation at 3000rpm, and then putting the 96-hole plate on a PCR instrument for performing thermal cycling at 95 ℃ for 2 minutes; 45 cycles of 95 ℃ for 30 seconds, 56 ℃ for 30 seconds and 72 ℃ for 60 seconds; 5 minutes at 72 ℃; keeping the temperature at 4 ℃.
TABLE 8
(3) SAP purification: the SAP mix was prepared in a 1.5 μ L tube according to table 9(SAP mix ratio table), then 2 μ L of SAP mix was added to each well, sealed and shaken well mixed, placed in a PCR instrument and subjected to the following procedures, i.e. 40 minutes at 37 ℃, 5 minutes at 85 ℃ and incubation at 4 ℃.
TABLE 9
(4) Single base extension: single-base extension mixtures were prepared according to the following Table 10 (Single-base extension mixture ratio Table), and the PCR procedure was as follows.
PCR procedure:
(5) resin purification: 41ul of water was added to each well of the sample plate and centrifuged, 15mg of clean resin was added, the plate was sealed with a membrane and placed on a rotator and shaken upside down for 15 minutes, and then centrifuged at 4000rpm for 5 minutes.
(6) Spotting onto a chip: the main parameter settings use the parameter settings recommended by the instrument.
(7) MALDI-TOF (matrix assisted laser Desorption ionization-time of flight) mass spectrometry detection: the main parameter set, ChipLinker software chemistry, was set to iPLEX and Gentop + Area was selected.
The above (1) to (7) can be adjusted by those skilled in the art according to the requirement, and can be implemented according to the basic knowledge in the art, wherein (6) and (7) can also be directly implemented according to
The instructions for the genotyping system (Agena, USA).
3. The typing results obtained for both samples are shown in table 11.
TABLE 11
And (3) obtaining the analysis result of the population genetic components of the sample 1 and the sample 2 by using STRUCTURE software for the genotype of each specific site. The CTT genetic component of the sample 1 accounts for 99.6 percent, the genetic components of other 26 people groups are less than 1 percent, and the analysis result of the genetic components of the groups supports that the sample 1 is the Tibetan individual of the Tibetan nationality of the Tibet plateau of China; the CTT genetic component of the sample 2 is less than 1 percent, the genetic components of other 26 people groups account for 99.4 percent, and the analysis result of the group genetic components supports that the sample 2 is the individuals of the Tibetan group of the non-Chinese Tibetan plateau (namely, the individuals of one of the other 26 groups).
The same procedure was used to perform the above analysis on the remaining 2686 samples, and the results are shown in fig. 1, and according to the distribution of yellow and red, it can be seen that, at the individual level, 87% of individual component 1 (yellow) in the CTT population is more than 90%, 98% of individual component 1 in other east asia is less than 10%, and 99%, 78%, 99%, 98% of individual component 1 in european, african, south asia and american populations are less than 10%, respectively.
The invention also provides a genetic component analysis result map of 27 population levels, which is displayed by a pie chart, as shown in fig. 2, the CTT is different from the genetic main components of the rest 26 populations, the genetic main component of the CTT is component 1, accounting for 73 percent (yellow), the genetic main component of the rest populations (namely the rest 26 populations) in the world is component 2 (red), and the proportion of the component 1 is less than 7 percent.
In conclusion, the genetic component analysis result obtained by the method of the invention is consistent with the known population source of the sample, which shows that the method and the system of the invention can identify the individual as the Chinese Qinghai-Tibet plateau family population individual or the non-Chinese Qinghai-Tibet plateau family population individual in 27 populations, and can increase the resolution ratio aiming at the east Asia population, thereby playing an important role in determining the population identity source of the involved personnel, promoting the rapid qualification of cases, determining the detection direction and the like.
Sequence listing
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<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>10
acgttggatg caacaccttt ctgcactctc 30
<210>11
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>11
acgttggatg aaaggttatg gacacactgc 30
<210>12
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>12
acgttggatg tactgttgct ctaagctgcc 30
<210>13
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>13
acgttggatg gaatgcctga gtcctttcac 30
<210>14
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>14
acgttggatg acatttgcct gaaccactcc 30
<210>15
<211>31
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>15
acgttggatg gttttgcttt gagatgggtt c 31
<210>16
<211>29
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>16
acgttggatg tgcagtgagc catgcacca 29
<210>17
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>17
acgttggatg agcctgagtt atgtaaggac 30
<210>18
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>18
acgttggatg agttcctcgg tttctctagc 30
<210>19
<211>31
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>19
acgttggatg ggcacttgaa attgtgcaag a 31
<210>20
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>20
acgttggatg ctctctgctc tccctttatc 30
<210>21
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>21
acgttggatg gaacttctct gtccaatatg 30
<210>22
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>22
acgttggatg gagaaaccga ggaactgaac 30
<210>23
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>23
acgttggatg tgtataggaa aagccacctc 30
<210>24
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>24
acgttggatg tctaccaacg tgtaacagac 30
<210>25
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>25
acgttggatg actgccactc tctggttttg 30
<210>26
<211>29
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>26
acgttggatg ttcaaagctc ttgatgtag 29
<210>27
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>27
acgttggatg gaacagaaag tgaactgaac 30
<210>28
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>28
acgttggatg gggtcttatc ctttcagttg 30
<210>29
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>29
acgttggatg gccttcactg tacctttgtc 30
<210>30
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>30
acgttggatg gttattgggg tcaaatttac 30
<210>31
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>31
acgttggatg ctgaggagac ccttaagttg 30
<210>32
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>32
acgttggatg tttcccccag aatcctgaac 30
<210>33
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>33
acgttggatg aaggactaac attgcccagc 30
<210>34
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>34
acgttggatg agctcgagct ctgtctcctt 30
<210>35
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>35
acgttggatg aacccttcct ggttgagtag 30
<210>36
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>36
acgttggatg ctgaaccaga gtcagtaacc 30
<210>37
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>37
acgttggatg atgcccagga aaacaaagcg 30
<210>38
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>38
acgttggatg agttcccatg atgctggcct 30
<210>39
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>39
acgttggatg gtttaagcac agacgggttc 30
<210>40
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>40
acgttggatg caggtagagc cacctttgtc 30
<210>41
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>41
acgttggatg gggaaataca ccacatccca 30
<210>42
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>42
acgttggatg ggtttcctgg ctttgtgaag 30
<210>43
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>43
acgttggatg aagggctctg ggcctataac 30
<210>44
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>44
acgttggatg gtcacttagg gcaggaatca 30
<210>45
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>45
acgttggatg tgcctgtatt aaaggtgtgg 30
<210>46
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>46
acgttggatg atgaaacagt ggcacaggtc 30
<210>47
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>47
acgttggatg catgtatcac ttcgcttcag 30
<210>48
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>48
acgttggatg cctgcctaaa tgcaggtttg 30
<210>49
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>49
acgttggatg actgattagc tgggttgctc 30
<210>50
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>50
acgttggatg gacaggtact gtcactgttc 30
<210>51
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>51
acgttggatg acagactatt gtgaggaggg 30
<210>52
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>52
acgttggatg tccatgtctg acccttccac 30
<210>53
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>53
acgttggatg gctgggttcc tggttcagta 30
<210>54
<211>29
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>54
acgttggatg ctatgagtag cagggtcag 29
<210>55
<211>29
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>55
acgttggatg gttggaactt ttcaaaagg 29
<210>56
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>56
acgttggatg agggtgtgaa acctgtgaac 30
<210>57
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>57
acgttggatg accagaacac acacaggtag 30
<210>58
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>58
acgttggatg caaaggttgg cctcgaaaag 30
<210>59
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>59
acgttggatg ggaacccctg tgtgctaaac 30
<210>60
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>60
acgttggatg acaactttca acaggctccg 30
<210>61
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>61
acgttggatg aagggtacag tggtttacag 30
<210>62
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>62
acgttggatg gtaaatcctc ataatgcccc 30
<210>63
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>63
acgttggatg gagagccaca ctgttctgta 30
<210>64
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>64
acgttggatg cgcctccaaa ttgaagtcct 30
<210>65
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>65
acgttggatg agttggacct tgaaggatcg 30
<210>66
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>66
acgttggatg aactgtggcc tacctcaaca 30
<210>67
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>67
acgttggatg attccaccaa taagtgaggc 30
<210>68
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>68
acgttggatg agcgtttata cattttcccc 30
<210>69
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>69
acgttggatg acactaatgt gagtacagcc 30
<210>70
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>70
acgttggatg tcttgcacaa cttccccatc 30
<210>71
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>71
acgttggatg taggtgaaaa tcctgaaatc 30
<210>72
<211>31
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>72
acgttggatg caaatagaac acttgcaaca c 31
<210>73
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>73
acgttggatg ccaaaaatag gaaacaaccc 30
<210>74
<211>29
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>74
acgttggatg gttcacttca ttttactgc 29
<210>75
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>75
acgttggatg gcacgctaat tgcagaaaag 30
<210>76
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>76
acgttggatg cttaccttaa cctacttagc 30
<210>77
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>77
acgttggatg ccaatatata ccacgctacc 30
<210>78
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>78
acgttggatg aaaagggtct gcccttttcc 30
<210>79
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>79
acgttggatg gaggttttca ttcacttcgg 30
<210>80
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>80
acgttggatg ttctggccag tcttagcaac 30
<210>81
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>81
acgttggatg gccagagcat tttaattgag 30
<210>82
<211>29
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>82
acgttggatg tctacaaggt atttgcaac 29
<210>83
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>83
acgttggatg atctcagata cccaaaggac 30
<210>84
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>84
acgttggatg gcaagcttat aggttgtgag 30
<210>85
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>85
acgttggatg gtaggattag acacatgccc 30
<210>86
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>86
acgttggatg gcatggattt ggaatcggag 30
<210>87
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>87
acgttggatg cttaaatgtg tatatagctc 30
<210>88
<211>31
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>88
acgttggatg gccaaacttc taaataaaga c 31
<210>89
<211>31
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>89
acgttggatg gaaagcagcc atagaaatac g 31
<210>90
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>90
acgttggatg cagaccacca cccatttttg 30
<210>91
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>91
acgttggatg ctaacttgaa tggagtgtgg 30
<210>92
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>92
acgttggatg tctccctccc tccagaattg 30
<210>93
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>93
acgttggatg tagtagtagc tagcacagcc 30
<210>94
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>94
acgttggatg gcttgctgac acttacttcc 30
<210>95
<211>29
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>95
acgttggatg tttttttcag cagacccac 29
<210>96
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>96
acgttggatg gtggcaagcc tgacagatga 30
<210>97
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>97
acgttggatg tgctggctta cctgtaaagt 30
<210>98
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>98
acgttggatg cacagggttt tgtttaaaag 30
<210>99
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>99
acgttggatg gcaaccagtt acgaactgtc 30
<210>100
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>100
acgttggatg cctgaagatc aggaatcatc 30
<210>101
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>101
acgttggatg tatcagtcat tgaccaggcg 30
<210>102
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>102
acgttggatg aaactcttgg gctcaagcag 30
<210>103
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>103
acgttggatg gagaaaacgt aagcagggac 30
<210>104
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>104
acgttggatg agatctccaa gtgatctccc 30
<210>105
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>105
acgttggatg tgcccagtcc aacagtatag 30
<210>106
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>106
acgttggatg gaaaacgaat tgggctggag 30
<210>107
<211>31
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>107
acgttggatg gttgtgtagc tcttaacatc g 31
<210>108
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>108
acgttggatg tgctccatct ggaaaggtca 30
<210>109
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>109
acgttggatg cgattactaa gcagcccatc 30
<210>110
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>110
acgttggatg acagcttttc ccagctgggc 30
<210>111
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>111
acgttggatg aagaggcgaa atgtgcagac 30
<210>112
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>112
acgttggatg tctgccattg tcttgcattg 30
<210>113
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>113
acgttggatg gtctgtaaat catcctgggc 30
<210>114
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>114
acgttggatg tgagccatct ctcgtctttg 30
<210>115
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>115
acgttggatg gtataacaac caaaggcctg 30
<210>116
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>116
acgttggatg tatagactcg gctcttgtcc 30
<210>117
<211>29
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>117
acgttggatg tgctgctcag caagcccca 29
<210>118
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>118
acgttggatg agggagggaa ggaaatgcag 30
<210>119
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>119
acgttggatg ctgtgagaaa cacgtgactg 30
<210>120
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>120
acgttggatg agaaacctcc atggctttac 30
<210>121
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>121
acgttggatg gagtaggact aagaaccagc 30
<210>122
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>122
acgttggatg cagcacttag tcccttcttc 30
<210>123
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>123
acgttggatg gcaacgccat gatgaatctc 30
<210>124
<211>31
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>124
acgttggatg gtggaattgt ataggagtat c 31
<210>125
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>125
acgttggatg cactccacag acaggataga 30
<210>126
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>126
acgttggatg taagccttgt gttttcctca 30
<210>127
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>127
acgttggatg ttctttgtct cctcccacac 30
<210>128
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>128
acgttggatg tctcaccctt tggaaagacc 30
<210>129
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>129
acgttggatg cacaaaaacc ttttaggccc 30
<210>130
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>130
acgttggatg aatgaactag gagaagaggg 30
<210>131
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>131
acgttggatg tttagcacat ggtgaacctg 30
<210>132
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>132
acgttggatg caaaatgctc ttgctgtccc 30
<210>133
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>133
acgttggatg ctgaccaaga gttgatgctg 30
<210>134
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>134
acgttggatg tcggaatcga cagactggtg 30
<210>135
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>135
acgttggatg tgaagggtag ccaccttctg 30
<210>136
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>136
acgttggatg gatcaccact gtaccagatg 30
<210>137
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>137
acgttggatg gtgaggatca aatgagacca 30
<210>138
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>138
acgttggatg cagctatgtc acctttctca 30
<210>139
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>139
acgttggatg catatgaaca cagcctggag 30
<210>140
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>140
acgttggatg agctaattag gcagccaacc 30
<210>141
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>141
acgttggatg catcatcagg ctccacagtc 30
<210>142
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>142
acgttggatg ggagggagta tttagatacc 30
<210>143
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>143
acgttggatg gtacaagggt gtaagcatgg 30
<210>144
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>144
acgttggatg gctactcgga agctgagatg 30
<210>145
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>145
acgttggatg aagctaaggg tgaagccaag 30
<210>146
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>146
acgttggatg cttctgcttt cccatcctac 30
<210>147
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>147
acgttggatg gaggagactg gaatttagag 30
<210>148
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>148
acgttggatg attccattcc accacacaac 30
<210>149
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>149
acgttggatg cagactggtt taaggccatc 30
<210>150
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>150
acgttggatg tgtggatgac tttgggactc 30
<210>151
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>151
acgttggatg gggtcagatg catggcataa 30
<210>152
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>152
acgttggatg tcctgtggat gtcttggaac 30
<210>153
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>153
acgttggatg tagtgacaac agaggtatcc 30
<210>154
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>154
acgttggatg caggcctcat atataccacc 30
<210>155
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>155
acgttggatg tcaacctgga tcatatcctc 30
<210>156
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>156
acgttggatg ggctgtttgg caactataag 30
<210>157
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>157
acgttggatg tgggtgcagc ctaggaaaac 30
<210>158
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>158
acgttggatg ctagataggg caggatactc 30
<210>159
<211>31
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>159
acgttggatg gggaataaca atttccatct c 31
<210>160
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>160
acgttggatg tgtcttggag cagaaagaag 30
<210>161
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>161
acgttggatg cctgaactca acagcagagc 30
<210>162
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>162
acgttggatg ggaaggttat agttctgtgc 30
<210>163
<211>29
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>163
acgttggatg agctgcaatc ctcacgtgg 29
<210>164
<211>29
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>164
acgttggatg aagagtggtg ctcaccctg 29
<210>165
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>165
acgttggatg tttctgcagc atctagagcc 30
<210>166
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>166
acgttggatg gaagatgaga gaagagtggc 30
<210>167
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>167
acgttggatg atcctacaca aactgatctc 30
<210>168
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>168
acgttggatg actgtggttt tagtggaagg 30
<210>169
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>169
acgttggatg aagaccttgc tttgccagag 30
<210>170
<211>29
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>170
acgttggatg ccagtggcct tgaggtttg 29
<210>171
<211>29
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>171
acgttggatg aactatacaa agtgctgag 29
<210>172
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>172
acgttggatg ccaggttaca gattgctgtt 30
<210>173
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>173
acgttggatg agtacgttca tattgttggg 30
<210>174
<211>30
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>174
acgttggatg cagagtttcc atttgagatg 30
<210>175
<211>22
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>175
ccgaggaact gaactttata ca 22
<210>176
<211>20
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>176
<210>177
<211>18
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>177
ctcagagagc tgacccac 18
<210>178
<211>18
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>178
gtggtagagg agccatac 18
<210>179
<211>19
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>179
ggcaagagca ttaaggtgt 19
<210>180
<211>19
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>180
ctcactcccc tttattctg 19
<210>181
<211>22
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>181
ttccctcctc aaaccccgtg aa 22
<210>182
<211>15
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>182
<210>183
<211>19
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>183
cactgcagaa agttctatg 19
<210>184
<211>19
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>184
cccactgagg ctcctctgc 19
<210>185
<211>24
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>185
aaatgtaaat atccacactt ggct 24
<210>186
<211>16
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>186
gactgtctgt gacagg 16
<210>187
<211>24
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>187
cttttgtcat ttttgtcata taga 24
<210>188
<211>22
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>188
ttcttttttt cctcaactta ca 22
<210>189
<211>26
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>189
gtcggggtca aatttacctt caataa 26
<210>190
<211>21
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>190
taagaatcct gaacaacttg a 21
<210>191
<211>25
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>191
cccctgtctc cttcctcagc tataa 25
<210>192
<211>19
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>192
agtcagtaac caatcctag 19
<210>193
<211>20
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>193
<210>194
<211>15
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>194
<210>195
<211>21
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>195
gtacaactca aatgttcttc a 21
<210>196
<211>20
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>196
cctttatatc acccccagtg 20
<210>197
<211>17
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>197
cctgcacccg gcaatac 17
<210>198
<211>19
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>198
cgtccagcca gaaaaaatc 19
<210>199
<211>19
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>199
cctctggaaa cagacactt 19
<210>200
<211>17
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>200
aacccttcca cgcctgt 17
<210>201
<211>22
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>201
ggaacttttt agaaaaacag at 22
<210>202
<211>20
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>202
<210>203
<211>16
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>203
gagttaggct ggactc 16
<210>204
<211>23
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>204
cccactgtgg ccactctggt cct 23
<210>205
<211>16
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>205
aagcactcca gtgaga 16
<210>206
<211>17
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>206
ctttcgggac ctccatc 17
<210>207
<211>20
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>207
<210>208
<211>23
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>208
tacattttcc ccattatttg tat 23
<210>209
<211>26
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>209
aaccacttcc ccatcttgct tttaac 26
<210>210
<211>25
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>210
taacttgcaa cactttaatg aatac 25
<210>211
<211>23
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>211
tagtatctca ttgtatgatg aag 23
<210>212
<211>17
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>212
acctacttag cctgagt 17
<210>213
<211>27
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>213
gccggtgttt ttctccttaa ccatttc 27
<210>214
<211>15
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>214
<210>215
<211>25
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>215
ccacaaggta tttgcaacct attat 25
<210>216
<211>20
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>216
<210>217
<211>23
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>217
gagagatcta gttcctaatc ttg 23
<210>218
<211>27
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>218
ccccaccaaa acacttctaa tggtaaa 27
<210>219
<211>18
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>219
ggaacacagt cacactta 18
<210>220
<211>15
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>220
<210>221
<211>23
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>221
agcaacaaga aaaaagttat atg 23
<210>222
<211>16
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>222
tgacagatga cctgga 16
<210>223
<211>26
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>223
gaaaagatga tacaaccatt ttggaa 26
<210>224
<211>15
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>224
<210>225
<211>22
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>225
<210>226
<211>15
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>226
<210>227
<211>21
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>227
acgcctgcct ttctttcttc t 21
<210>228
<211>19
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>228
agctcaccct aaagataaa 19
<210>229
<211>21
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>229
ttcaacgtgt cctccggtta c 21
<210>230
<211>22
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>230
aaagtattta tggagcatat ct 22
<210>231
<211>15
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>231
<210>232
<211>22
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>232
aggatcttgt cccagaccaa aa 22
<210>233
<211>15
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>233
<210>234
<211>22
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>234
gcttattgga caatgattgt ag 22
<210>235
<211>15
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>235
<210>236
<211>25
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>236
gattgtatag gagtatctac tcttg 25
<210>237
<211>27
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>237
ttactgtgtt ttcctcaaat actacat 27
<210>238
<211>16
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>238
tacagcagct ccagga 16
<210>239
<211>23
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>239
<210>240
<211>25
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>240
tctggaccaa aattcaaaag gactt 25
<210>241
<211>18
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>241
taaactgctg taaggtga 18
<210>242
<211>20
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>242
<210>243
<211>26
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>243
ggacaggaaa catatttatt gagcac 26
<210>244
<211>24
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>244
aggttactgg tgacagacat cttg 24
<210>245
<211>17
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>245
tacagctcct aactccc 17
<210>246
<211>17
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>246
ggcaggatag cttgagt 17
<210>247
<211>24
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>247
ccccccatcc tacaagataa aagg 24
<210>248
<211>17
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>248
actatttgta gaggggc 17
<210>249
<211>19
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>249
gcctttggga ctcagagag 19
<210>250
<211>27
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>250
ccttgggatg tcttggaaca gtgggat 27
<210>251
<211>19
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>251
ggatatacca ccccactca 19
<210>252
<211>25
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>252
ttgttcttgg tgatttaatt tttgc 25
<210>253
<211>17
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>253
acaagtgtgg gtggttc 17
<210>254
<211>23
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>254
<210>255
<211>20
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>255
<210>256
<211>17
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>256
tcagcccctt ttagatg 17
<210>257
<211>18
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>257
gaagagtggc aaagagtt 18
<210>258
<211>25
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>258
cgtaaaggat atgtgattga tgtag 25
<210>259
<211>19
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>259
accttgaggt ttgtcagaa 19
<210>260
<211>22
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>260
cctttaaaat tgtgtctgat tc 22
<210>261
<211>20
<212>DNA
<213> Artificial sequence
<220>
<223> primer
<400>261
Claims (9)
1. A method for identifying individuals of the Tibetan nationality of the tibetan plateau of china among 27 populations, the method comprising:
1) extracting DNA of an individual;
2) obtaining genotypes of 87 Tibetan specific plateau adaptive SNP sites of the DNA, wherein the 87 Tibetan specific plateau adaptive SNP sites are as follows: rs10178633, rs10193827, rs10206434, rs1109285, rs1109286, rs11122280, rs11125068, rs115321619, s116062164, rs116611511, rs11675232, rs11675441, rs11689011, rs11805033, rs12467821, rs13003074, rs13006131, rs13419896, rs13424253, rs1374749, rs141366568, rs141426873, rs 1428201, rs1447563, rs 149391 391, rs 1507473, rs 15624242424563, rs 170950, rs 35010, rs 35017017013, rs1867784, rs 8092, rs1992846, rs2066140, rs2121266, rs2153364, rs 22449279, rs2275279, rs2486729, rs 8686241867356355635563556355639, rs 755635563556355635563556355635563556355648, rs 75563556355635563556355635563556355635563556355648, rs 7556355635563556355635563556355635563556355648, rs 75563556355635563556355635563556355648, rs 75563556355648, rs 755635563556355635563556355648, rs 755648, rs 7556375637563756375648, rs 75563756375637563756375637563756375637563756375648, rs 7556375637563756375648, rs 7556375637563756375637563756375637563756375637563756375648, rs 75563756375637563756375648, rs 755637563756375637563756375637563756375637563756375637563756;
3) obtaining the group genetic component analysis result of the individual by using STRUCTURE software according to the genotype of the Tibetan specific plateau adaptive SNP locus, and identifying whether the individual is a Tibetan individual of the Tibetan of the Qinghai-Tibet plateau or not according to the result;
the 27 populations are the western population of Omobia, the Mende population of Seralane, the Jolaba population of Ileba of Nigeria, the West population of Kenya Luysia, the Eisen population of Nigeria, the Black population of American, the Balados island population of Caribbean, the Peru Lema population, the Columbia population of Golomis Mderlin, the populations of Podochiza, the Mexico population of los Angeles, the northern Europe and Western Africa of Utah, the Finland population of Finland, the British population of Scotland and England, the Ibiya population of Spanish, the Talania population of Italy, the Mengla population of Menglatiria, the Pakistan population of Beijing, the North Kyokura population of Texas Houston, the Indian Han Guugen population, the Smith population of Milan, the Japanese Pigeon, the Beijing Hodgkin of Beijing of Texas, Chinese southern Han people, Vietnam Jing people, Chinese Xishuangbanna Dai people and Chinese Qinghai-Tibet plateau Tibetan people.
2. The method as claimed in claim 1, wherein 2) comprises the steps of amplifying 87 pairs of amplification primers corresponding to the 87 Tibetan specific plateau adaptive SNP sites one by one to obtain amplification products; and a step of extending the amplification product by using 87 extension primers which are in one-to-one correspondence with the 87 Tibetan specific plateau adaptive SNP sites to obtain an extension product.
3. The method of claim 2, wherein the amplification primer is a nucleotide sequence of SEQ ID No.1 to SEQ ID No.174 of the sequence listing; the extension primer is a nucleotide sequence from SEQ ID No.175 to SEQ ID No.261 in the sequence table.
4. The method as claimed in claim 3, wherein 2) further comprises the step of analyzing the extension products using a genotyping system after obtaining the extension products to obtain genotypes of the 87 Tibetan specific plateau adaptive SNP sites.
5. A system for identifying individuals of Tibetan nationality of Tibet plateau in 27 populations is characterized by comprising a DNA extraction system, a multiplex amplification detection system and a data acquisition system;
the DNA extraction system is used for extracting DNA of the individual;
the composite amplification detection system is used for obtaining genotypes of 87 Tibetan specific plateau adaptive SNP sites of the DNA, wherein the 87 Tibetan specific plateau adaptive SNP sites are as follows: rs10178633, rs10193827, rs10206434, rs1109285, rs1109286, rs11122280, rs11125068, rs115321619, s116062164, rs116611511, rs11675232, rs11675441, rs11689011, rs11805033, rs12467821, rs13003074, rs13006131, rs13419896, rs13424253, rs1374749, rs141366568, rs141426873, rs 1428201, rs1447563, rs 149391 391, rs 1507473, rs 15624242424563, rs 170950, rs 35010, rs 35017017013, rs1867784, rs 8092, rs1992846, rs2066140, rs2121266, rs2153364, rs 22449279, rs2275279, rs2486729, rs 8686241867356355635563556355639, rs 755635563556355635563556355635563556355648, rs 75563556355635563556355635563556355635563556355648, rs 7556355635563556355635563556355635563556355648, rs 75563556355635563556355635563556355648, rs 75563556355648, rs 755635563556355635563556355648, rs 755648, rs 7556375637563756375648, rs 75563756375637563756375637563756375637563756375648, rs 7556375637563756375648, rs 7556375637563756375637563756375637563756375637563756375648, rs 75563756375637563756375648, rs 755637563756375637563756375637563756375637563756375637563756;
the data acquisition system is used for acquiring a group genetic component analysis result of the individual by using STRUCTURE software according to the genotype of the SNP site adapted to the specific plateau of each Tibetan, and identifying whether the individual is the Tibetan individual of the Tibetan of the Qinghai-Tibet plateau of China according to the result;
the 27 populations are the western Oncbia population, Mende population of Seralane, the Joluba population of Ilbardane, Nigeria Lusiya, Nigeria Eisen, American Black population, Caribbean Paudo, Peru horse, Columbia populations of Melibean, Podochius populations, Mexico populations of los Angeles, northern Europe and Western European Africa of Utah, Finland populations of Finland, England of Scotland and England of England
National population, the illibia population of spain, the talcany population of italy, the bangla population of bangla, the bystander population of pakistan, the ancient gillat system indian population of houston, texas, the indian tylugu population of uk, the strikana tilmir population of uk, the japanese population of tokyo, the beijing chinese han population, the southern han population of china, the vietnam kyo population of china, the western double banna dai population of china, and the qinghai tibet high school population of china.
6. The system of claim 5, wherein the multiplex amplification detection system is configured to amplify the 87 Tibetan specific plateau adaptive SNP sites with 87 pairs of amplification primers corresponding to the 87 Tibetan specific plateau adaptive SNP sites one by one to obtain amplification products, extend the amplification products with 87 extension primers corresponding to the 87 Tibetan specific plateau adaptive SNP sites one by one to obtain extension products, and obtain genotypes of the 87 Tibetan specific plateau adaptive SNP sites of the individual's DNA from the obtained extension products.
7. The system of claim 6, wherein the amplification primer is a nucleotide sequence of SEQ ID No.1 to SEQ ID No.174 of the sequence Listing, and the extension primer is a nucleotide sequence of SEQ ID No.175 to SEQ ID No.261 of the sequence Listing.
8. A composite amplification detection system is characterized in that the system comprises individual DNA, 87 Tibetan specific plateau adaptive SNP sites, amplification primers and extension primers,
the composite amplification detection system is used for amplifying 87 Tibetan specific plateau adaptive SNP sites of the DNA of the individual by using an amplification primer to obtain an amplification product, extending the amplification product by using an extension primer, and obtaining the genotype of the 87 Tibetan specific plateau adaptive SNP sites of the DNA of the individual from the obtained extension product;
the 87 Tibetan specific plateau adaptive SNP loci are as follows: rs10178633, rs10193827, rs10206434, rs1109285, rs1109286, rs11122280, rs11125068, rs115321619, s116062164, rs116611511, rs11675232, rs11675441, rs11689011, rs11805033, rs12467821, rs13003074, rs13006131, rs13419896, rs13424253, rs1374749, rs141366568, rs141426873, rs 1428201, rs1447563, rs 149391 391, rs 1507473, rs 15624242424563, rs 170950, rs 35010, rs 35017017013, rs1867784, rs 8092, rs1992846, rs2066140, rs2121266, rs2153364, rs 22449279, rs2275279, rs2486729, rs 8624186735635794, rs 75563556355635563556355635563556355648, rs 7556355635563556355635563556355635563556355648, rs 755635563556355635563556355635563556355648, rs 7556355635563556355635563556355648, rs 75563556355635563556355648, rs 7556375648, rs 7556375637563756375648, rs 7556375637563756375637563756375648, rs 75563756375637563756375637563756375637563756375648, rs 75563756375637563756375637563756375648, rs 7556375637563756375637563756375648, rs 75563756375637563756375648, rs 7556375637563756375637563756375637563756375637563756375637;
the amplification primers consist of 87 pairs of amplification primers which correspond to the 87 Tibetan specific plateau adaptive SNP loci one by one, and the amplification primers are nucleotide sequences from SEQ ID No.1 to SEQ ID No.174 in a sequence table;
the extension primers consist of 87 extension primers which correspond to the 87 Tibetan specific plateau adaptive SNP loci one by one, and the extension primers are nucleotide sequences from SEQ ID No.175 to SEQ ID No.261 in a sequence table;
the 27 populations are the western population of Omobia, the Mende population of Seralane, the Jolaba population of Ileba of Nigeria, the West population of Kenya Luysia, the Eisen population of Nigeria, the Black population of American, the Balados island population of Caribbean, the Peru Lema population, the Columbia population of Golomis Mderlin, the populations of Podochiza, the Mexico population of los Angeles, the northern Europe and Western Africa of Utah, the Finland population of Finland, the British population of Scotland and England, the Ibiya population of Spanish, the Talania population of Italy, the Mengla population of Menglatiria, the Pakistan population of Beijing, the North Kyokura population of Texas Houston, the Indian Han Guugen population, the Smith population of Milan, the Japanese Pigeon, the Beijing Hodgkin of Beijing of Texas, Chinese southern Han people, Vietnam Jing people, Chinese Xishuangbanna Dai people and Chinese Qinghai-Tibet plateau Tibetan people.
9. A detection kit comprising the multiplex amplification detection system according to claim 8.
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| CN108546763B (en) * | 2018-04-04 | 2021-04-23 | 中国人民解放军空军特色医学中心 | Application of EPAS1 gene label mononucleotide in screening population suitable for plateau environment and screening method |
| CN108411008B (en) * | 2018-06-01 | 2021-07-27 | 公安部物证鉴定中心 | Application of 72 SNP sites and related primers in identification or assisted identification of human ethnic groups |
| CN109346124A (en) * | 2018-10-10 | 2019-02-15 | 深圳韦格纳医学检验实验室 | Genes location method based on SNP parting |
| CN111118168B (en) * | 2018-11-01 | 2022-04-29 | 中国科学院北京基因组研究所 | SNP marker combination for deducing main ethnic group of northwest China and adjacent middle and Asia countries |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146820A (en) * | 2013-02-22 | 2013-06-12 | 公安部物证鉴定中心 | Method and system used for deducing Han, Tibetan or Wei population source of individual with unknown source |
| CN104212886A (en) * | 2014-07-25 | 2014-12-17 | 公安部物证鉴定中心 | Method and system for performing African, European and East Asian population genetic principal component analysis to unknown-source individual |
| CN106480198A (en) * | 2016-11-01 | 2017-03-08 | 公安部物证鉴定中心 | A kind of method and system for carrying out individual identification to unknown sample |
| CN106755321A (en) * | 2015-11-28 | 2017-05-31 | 内蒙古中科正标生物科技有限责任公司 | The method for screening hypoxia adaptability sheep |
-
2017
- 2017-08-18 CN CN201710713359.7A patent/CN107400713B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146820A (en) * | 2013-02-22 | 2013-06-12 | 公安部物证鉴定中心 | Method and system used for deducing Han, Tibetan or Wei population source of individual with unknown source |
| CN104212886A (en) * | 2014-07-25 | 2014-12-17 | 公安部物证鉴定中心 | Method and system for performing African, European and East Asian population genetic principal component analysis to unknown-source individual |
| CN106755321A (en) * | 2015-11-28 | 2017-05-31 | 内蒙古中科正标生物科技有限责任公司 | The method for screening hypoxia adaptability sheep |
| CN106480198A (en) * | 2016-11-01 | 2017-03-08 | 公安部物证鉴定中心 | A kind of method and system for carrying out individual identification to unknown sample |
Non-Patent Citations (4)
| Title |
|---|
| Genetic signatures of high-altitude adaptation in Tibetans;Jian Yang，et al.;《PNAS》;20170418;第114卷(第16期);第4189-4194页 * |
| Genetic Variations in Tibetan Populations and High-Altitude Adaptation at the Himalayas;Yi Peng，et al.;《Mol. Biol. Evol.》;20101028;第28卷(第2期);第1075-1081页 * |
| 三个不同海拔藏族群体HIF1A基因九个单核苷酸多态性位点研究;柯金坤等;《中华医学遗传学杂志》;20101031;第27卷(第5期);第584-589页 * |
| 藏族高原适应性相关研究及法医学应用展望;黄美莎等;《刑事技术》;20181215;第43卷(第6期);第481-484页 * |
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