MD1015Z - Method for decellularization of liver in experimental animals - Google Patents

Method for decellularization of liver in experimental animals

Info

Publication number
MD1015Z
MD1015Z MDS20150065A MDS20150065A MD1015Z MD 1015 Z MD1015 Z MD 1015Z MD S20150065 A MDS20150065 A MD S20150065A MD S20150065 A MDS20150065 A MD S20150065A MD 1015 Z MD1015 Z MD 1015Z
Authority
MD
Moldova
Prior art keywords
experimental animals
perfused
hours
concentration
decellularization
Prior art date
Application number
MDS20150065A
Other languages
Romanian (ro)
Russian (ru)
Inventor
Александру УРСУ
Виорел НАКУ
Марианна ЖИАН
Ольга МАКАГОНОВА
Адриан КОЧУГ
Виорика САРМАНЮК
Ольга ИГНАТОВ
Original Assignee
ОП ГОСУДАРСТВЕННЫЙ МЕДИЦИНСКИЙ И ФАРМАЦЕВТИЧЕСКИЙ УНИВЕРСИТЕТ им. НИКОЛАЯ ТЕСТЕМИЦАНУ РЕСПУБЛИКИ МОЛДОВА
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by ОП ГОСУДАРСТВЕННЫЙ МЕДИЦИНСКИЙ И ФАРМАЦЕВТИЧЕСКИЙ УНИВЕРСИТЕТ им. НИКОЛАЯ ТЕСТЕМИЦАНУ РЕСПУБЛИКИ МОЛДОВА filed Critical ОП ГОСУДАРСТВЕННЫЙ МЕДИЦИНСКИЙ И ФАРМАЦЕВТИЧЕСКИЙ УНИВЕРСИТЕТ им. НИКОЛАЯ ТЕСТЕМИЦАНУ РЕСПУБЛИКИ МОЛДОВА
Priority to MDS20150065A priority Critical patent/MD1015Z/en
Publication of MD1015Y publication Critical patent/MD1015Y/en
Publication of MD1015Z publication Critical patent/MD1015Z/en

Links

Abstract

The invention relates to molecular biology, in particular to a method for decellularization of liver in experimental animals.According to the invention, experimental animals are euthanized in the room with carbon dioxide with the concentration of 5%, are fixed on the worktable, is delimited the operative field, is opened the abdominal cavity by the incision of midline of the body, is mobilized the portal vein and is ligated to the proximal end, is cannulated with a catheter, through which is perfused a solution containing distilled water in an amount of 1…1.5 L and 63 mL of citrate-phosphate-dextrose for 4…6 hours, then is perfused a sodium dodecyl sulfate solution with the concentration of 0.25% in an amount ofn 2…3 L, for 12…15 hours, afterwards is perfused a phosphate buffer solution with the concentration of 1% in a volume of 1.5 L, for 2…3 hours.
MDS20150065A 2015-05-12 2015-05-12 Method for decellularization of liver in experimental animals MD1015Z (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MDS20150065A MD1015Z (en) 2015-05-12 2015-05-12 Method for decellularization of liver in experimental animals

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MDS20150065A MD1015Z (en) 2015-05-12 2015-05-12 Method for decellularization of liver in experimental animals

Publications (2)

Publication Number Publication Date
MD1015Y MD1015Y (en) 2016-03-31
MD1015Z true MD1015Z (en) 2016-10-31

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MDS20150065A MD1015Z (en) 2015-05-12 2015-05-12 Method for decellularization of liver in experimental animals

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MD (1) MD1015Z (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD1171Z (en) * 2016-12-23 2018-02-28 Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова Method for treating the decellularized liver matrix to enhance cell adhesion

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD191Z5 (en) * 2010-01-21 2010-11-30 Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова Nutrient medium for the cultivation of cells of animal origin
RU2445940C1 (en) * 2010-11-03 2012-03-27 Федеральное государственное учреждение "Всероссийский центр глазной и пластической хирургии Федерального агентства по здравоохранению и социальному развитию" Method for producing allogenic transplants of cardiac vessels with valves
RU2453291C1 (en) * 2010-11-17 2012-06-20 Федеральное государственное бюджетное учреждение "Российский онкологический научный центр имени Н.Н. Блохина" Российской академии медицинских наук (ФГБУ "РОНЦ им. Н.Н. Блохина" РАМН) Method for making tracheal matrix for allogeneic transplantation
UA85240U (en) * 2013-06-04 2013-11-11 Государственное Учреждение "Институт Неотложной И Восстановительной Хирургии Им. В.К. Гусака Национальной Академии Медицинских Наук Украины" Method for preparation of decellularized matrix for cardiovascular grafts
RU2504334C1 (en) * 2012-11-28 2014-01-20 Федеральное государственное бюджетное учреждение "Федеральный Центр сердца, крови и Эндокринологии имени В.А. Алмазова" Министерства здравоохранения Российской Федерации Method of decellularisation of small-calibre blood vessels
RU2524619C2 (en) * 2013-04-02 2014-07-27 Государственное бюджетное учреждение здравоохранения города Москвы Научно-исследовательский институт скорой помощи имени Н.В. Склифосовского Департамента здравоохранения г. Москвы Method for making dermal matrix
RU2539918C1 (en) * 2013-11-29 2015-01-27 Федеральное государственное бюджетное учреждение "Федеральный научный центр трансплантологии и искусственных органов имени академика В.И. Шумакова" Министерства здравоохранения Российской Федерации Method for preparing tissue-specific matrix for tissue engineering of parenchymal organ
MD939Y (en) * 2015-04-17 2015-08-31 Ip Universitatea De Stat De Medicină Şi Farmacie "Nicolae Testemiţanu" Din Republica Moldova Device for liver retention in the decellularization process

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD191Z5 (en) * 2010-01-21 2010-11-30 Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова Nutrient medium for the cultivation of cells of animal origin
RU2445940C1 (en) * 2010-11-03 2012-03-27 Федеральное государственное учреждение "Всероссийский центр глазной и пластической хирургии Федерального агентства по здравоохранению и социальному развитию" Method for producing allogenic transplants of cardiac vessels with valves
RU2453291C1 (en) * 2010-11-17 2012-06-20 Федеральное государственное бюджетное учреждение "Российский онкологический научный центр имени Н.Н. Блохина" Российской академии медицинских наук (ФГБУ "РОНЦ им. Н.Н. Блохина" РАМН) Method for making tracheal matrix for allogeneic transplantation
RU2504334C1 (en) * 2012-11-28 2014-01-20 Федеральное государственное бюджетное учреждение "Федеральный Центр сердца, крови и Эндокринологии имени В.А. Алмазова" Министерства здравоохранения Российской Федерации Method of decellularisation of small-calibre blood vessels
RU2524619C2 (en) * 2013-04-02 2014-07-27 Государственное бюджетное учреждение здравоохранения города Москвы Научно-исследовательский институт скорой помощи имени Н.В. Склифосовского Департамента здравоохранения г. Москвы Method for making dermal matrix
UA85240U (en) * 2013-06-04 2013-11-11 Государственное Учреждение "Институт Неотложной И Восстановительной Хирургии Им. В.К. Гусака Национальной Академии Медицинских Наук Украины" Method for preparation of decellularized matrix for cardiovascular grafts
RU2539918C1 (en) * 2013-11-29 2015-01-27 Федеральное государственное бюджетное учреждение "Федеральный научный центр трансплантологии и искусственных органов имени академика В.И. Шумакова" Министерства здравоохранения Российской Федерации Method for preparing tissue-specific matrix for tissue engineering of parenchymal organ
MD939Y (en) * 2015-04-17 2015-08-31 Ip Universitatea De Stat De Medicină Şi Farmacie "Nicolae Testemiţanu" Din Republica Moldova Device for liver retention in the decellularization process

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Omar Barakat, Shahrzad Abbasi, Gabriela Rodriguez, Jessi Rios et al. Use of decellularized porcine liver for engineering humanized liver organ. Journal of surgical research, 2012, 173(1), p. 11-25 *

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Publication number Publication date
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