MD1015Y - Method for decellularization of liver in experimental animals - Google Patents
Method for decellularization of liver in experimental animalsInfo
- Publication number
- MD1015Y MD1015Y MDS20150065A MDS20150065A MD1015Y MD 1015 Y MD1015 Y MD 1015Y MD S20150065 A MDS20150065 A MD S20150065A MD S20150065 A MDS20150065 A MD S20150065A MD 1015 Y MD1015 Y MD 1015Y
- Authority
- MD
- Moldova
- Prior art keywords
- experimental animals
- perfused
- hours
- concentration
- decellularization
- Prior art date
Links
Abstract
The invention relates to molecular biology, in particular to a method for decellularization of liver in experimental animals.According to the invention, experimental animals are euthanized in the room with carbon dioxide with the concentration of 5%, are fixed on the worktable, is delimited the operative field, is opened the abdominal cavity by the incision of midline of the body, is mobilized the portal vein and is ligated to the proximal end, is cannulated with a catheter, through which is perfused a solution containing distilled water in an amount of 1…1.5 L and 63 mL of citrate-phosphate-dextrose for 4…6 hours, then is perfused a sodium dodecyl sulfate solution with the concentration of 0.25% in an amount ofn 2…3 L, for 12…15 hours, afterwards is perfused a phosphate buffer solution with the concentration of 1% in a volume of 1.5 L, for 2…3 hours.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MDS20150065A MD1015Z (en) | 2015-05-12 | 2015-05-12 | Method for decellularization of liver in experimental animals |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MDS20150065A MD1015Z (en) | 2015-05-12 | 2015-05-12 | Method for decellularization of liver in experimental animals |
Publications (2)
Publication Number | Publication Date |
---|---|
MD1015Y true MD1015Y (en) | 2016-03-31 |
MD1015Z MD1015Z (en) | 2016-10-31 |
Family
ID=55646054
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MDS20150065A MD1015Z (en) | 2015-05-12 | 2015-05-12 | Method for decellularization of liver in experimental animals |
Country Status (1)
Country | Link |
---|---|
MD (1) | MD1015Z (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MD1171Z (en) * | 2016-12-23 | 2018-02-28 | Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова | Method for treating the decellularized liver matrix to enhance cell adhesion |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MD191Z5 (en) * | 2010-01-21 | 2010-11-30 | Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова | Nutrient medium for the cultivation of cells of animal origin |
RU2445940C1 (en) * | 2010-11-03 | 2012-03-27 | Федеральное государственное учреждение "Всероссийский центр глазной и пластической хирургии Федерального агентства по здравоохранению и социальному развитию" | Method for producing allogenic transplants of cardiac vessels with valves |
RU2453291C1 (en) * | 2010-11-17 | 2012-06-20 | Федеральное государственное бюджетное учреждение "Российский онкологический научный центр имени Н.Н. Блохина" Российской академии медицинских наук (ФГБУ "РОНЦ им. Н.Н. Блохина" РАМН) | Method for making tracheal matrix for allogeneic transplantation |
RU2504334C1 (en) * | 2012-11-28 | 2014-01-20 | Федеральное государственное бюджетное учреждение "Федеральный Центр сердца, крови и Эндокринологии имени В.А. Алмазова" Министерства здравоохранения Российской Федерации | Method of decellularisation of small-calibre blood vessels |
RU2524619C2 (en) * | 2013-04-02 | 2014-07-27 | Государственное бюджетное учреждение здравоохранения города Москвы Научно-исследовательский институт скорой помощи имени Н.В. Склифосовского Департамента здравоохранения г. Москвы | Method for making dermal matrix |
UA85240U (en) * | 2013-06-04 | 2013-11-11 | Государственное Учреждение "Институт Неотложной И Восстановительной Хирургии Им. В.К. Гусака Национальной Академии Медицинских Наук Украины" | Method for preparation of decellularized matrix for cardiovascular grafts |
RU2539918C1 (en) * | 2013-11-29 | 2015-01-27 | Федеральное государственное бюджетное учреждение "Федеральный научный центр трансплантологии и искусственных органов имени академика В.И. Шумакова" Министерства здравоохранения Российской Федерации | Method for preparing tissue-specific matrix for tissue engineering of parenchymal organ |
MD939Z (en) * | 2015-04-17 | 2016-03-31 | ОП ГОСУДАРСТВЕННЫЙ МЕДИЦИНСКИЙ И ФАРМАЦЕВТИЧЕСКИЙ УНИВЕРСИТЕТ им. НИКОЛАЯ ТЕСТЕМИЦАНУ РЕСПУБЛИКИ МОЛДОВА | Device for liver retention in the decellularization process |
-
2015
- 2015-05-12 MD MDS20150065A patent/MD1015Z/en not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MD1171Z (en) * | 2016-12-23 | 2018-02-28 | Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова | Method for treating the decellularized liver matrix to enhance cell adhesion |
Also Published As
Publication number | Publication date |
---|---|
MD1015Z (en) | 2016-10-31 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG9Y | Short term patent issued | ||
KA4Y | Short-term patent lapsed due to non-payment of fees (with right of restoration) |