MA37434A1 - Probes and legislators for rapid and reliable quantification of prostate cancer genes using appropriately controlled genes - Google Patents

Probes and legislators for rapid and reliable quantification of prostate cancer genes using appropriately controlled genes

Info

Publication number
MA37434A1
MA37434A1 MA37434A MA37434A MA37434A1 MA 37434 A1 MA37434 A1 MA 37434A1 MA 37434 A MA37434 A MA 37434A MA 37434 A MA37434 A MA 37434A MA 37434 A1 MA37434 A1 MA 37434A1
Authority
MA
Morocco
Prior art keywords
genes
probes
prostate cancer
legislators
rapid
Prior art date
Application number
MA37434A
Other languages
Arabic (ar)
French (fr)
Other versions
MA37434B1 (en
Inventor
El Hassane Sefrioui
Abdeladim MOUMEN
Imane Abdellaoui-Maane
Original Assignee
Mascir (Morrocan Found For Advanced Science Innovation & Research)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mascir (Morrocan Found For Advanced Science Innovation & Research) filed Critical Mascir (Morrocan Found For Advanced Science Innovation & Research)
Priority to MA37434A priority Critical patent/MA37434B1/en
Priority to PCT/MA2015/000022 priority patent/WO2016060537A2/en
Publication of MA37434A1 publication Critical patent/MA37434A1/en
Publication of MA37434B1 publication Critical patent/MA37434B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne de nouveaux sets d'amorces et sondes et leur utilisation pour la détection des gènes spécifiques de la prostate à savoir les gènes pca3, tmprss2-erg et amacr et des gènes de contrôles choisis parmi les gènes rpl5, hprtl, et hnrnpalpour la normalisation des données d'analyse d'expression des gènes cibles à partir d'échantillons de patients que ce soit des échantillons tissulaires ou des fluides biologiques. Les sets d'amorces et sondes des gènes cibles et gènes de références sont plus sensibles que ceux citées en littérature et seront utilisées en pcr en temps réel (qpcr) en format simplex ou multiplex pour une quantification fiable, rapide et moins couteuse. La présente invention sera éventuellement à la base d'un kit pour le diagnostic moléculaire du cancer de la prostate à partir d'un échantillon biologique prélevé chez un patient selon lequel on détermine l'expression d'au moins un des gènes cibles (pca3, tmprss2-erg, amacr)en réaction simplex ou multiplex.The present invention relates to novel sets of primers and probes and their use for the detection of prostate-specific genes, namely the pca3, tmprss2-erg and amacr genes and control genes chosen from the rpl5, hprtl, and hnrnpal genes. standardization of target gene expression analysis data from patient samples whether tissue samples or biological fluids. The sets of primers and probes of target genes and reference genes are more sensitive than those mentioned in literature and will be used in real-time pcr (qpcr) in simplex or multiplex format for a reliable, fast and less expensive quantification. The present invention will eventually be the basis of a kit for the molecular diagnosis of prostate cancer from a biological sample taken from a patient according to which the expression of at least one of the target genes (pca3, tmprss2-erg, amacr) in simplex or multiplex reaction.

MA37434A 2014-10-17 2014-10-17 Probes and primers for rapid and reliable quantification of prostate cancer-specific genes using adequate control genes MA37434B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MA37434A MA37434B1 (en) 2014-10-17 2014-10-17 Probes and primers for rapid and reliable quantification of prostate cancer-specific genes using adequate control genes
PCT/MA2015/000022 WO2016060537A2 (en) 2014-10-17 2015-12-16 Probes and primers for the rapid and reliable quantification of the genes specific to prostate cancer, using suitable reference genes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MA37434A MA37434B1 (en) 2014-10-17 2014-10-17 Probes and primers for rapid and reliable quantification of prostate cancer-specific genes using adequate control genes

Publications (2)

Publication Number Publication Date
MA37434A1 true MA37434A1 (en) 2016-05-31
MA37434B1 MA37434B1 (en) 2017-01-31

Family

ID=55747527

Family Applications (1)

Application Number Title Priority Date Filing Date
MA37434A MA37434B1 (en) 2014-10-17 2014-10-17 Probes and primers for rapid and reliable quantification of prostate cancer-specific genes using adequate control genes

Country Status (2)

Country Link
MA (1) MA37434B1 (en)
WO (1) WO2016060537A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106636000A (en) * 2016-12-23 2017-05-10 广东圣赛生物科技有限公司 hESCs-TK cell line and building method and application thereof
CN110628908B (en) * 2019-09-19 2022-05-10 北京恩泽康泰生物科技有限公司 Biomarker and detection kit for prostate cancer diagnosis grading and benign and malignant prediction

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006032394B4 (en) * 2006-03-17 2011-07-07 Technische Universität Dresden, 01069 Method for diagnosis and differentiation of prostate cancer

Also Published As

Publication number Publication date
WO2016060537A3 (en) 2016-09-29
WO2016060537A2 (en) 2016-04-21
MA37434B1 (en) 2017-01-31

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