LV13022B - Method for biocomposting the organic waste in field conditions - Google Patents

Method for biocomposting the organic waste in field conditions Download PDF

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LV13022B
LV13022B LVP-01-175A LV010175A LV13022B LV 13022 B LV13022 B LV 13022B LV 010175 A LV010175 A LV 010175A LV 13022 B LV13022 B LV 13022B
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bacteria
composting
anaerobic
trichoderma
compost
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LVP-01-175A
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Francis Utinans
Dzidra Zarina
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Lazdonas Piensaimnieks As
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses

Abstract

The approach proposed herein refers to the activities in agriculture and protection of environment. In particular, it refers to the technology of organic fertiliser production by processing dairy factory waste (unused cheese whey) and forestry waste (wood-sawdust) using different kinds (or strains) of micro-organisms. Composting process can be realised in field conditions in windrows. The first step of biodegradation can be realised in anaerobic regime, the second step - in aerobic regime. To promote the composting process, the use of the following micro-organisms have been applied: methanogenic bacteria (Methanosarcina methanica sp.-O-16, OG-3, Methanobacterium sp.A-5, Methanococcus sp.-D-1, Methanotrix soehngenii K-2), cellulose- and lignin-splitting bacteria (Trichoderma viride-LL-333 and Trichoderma lignorum LL-751) as well as bacteria for nitrification association, regulating the circulation of nitrogen (Pseudomonas sp.-DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp. DN-3 and Sarcina sp.-13-N). A portion of ready compost could be used as "inoculum" for composting new-formed windrows of organic wastes.

Description

KĢPOSTA RAŽOŠANAS PAŅĒMIENSTHE MESSAGE MANUFACTURING TECHNIQUES

1. Izgudrojums attiecas uz apkārtējās vides aizsardzību un lauksaimniecību, konkrēti - uz organiskā mēslojuma ieguves tehnoloģijas izstrādi, pārstrādājot piensaimniecības (neizmantotās sūkaliņas) un mežsaimniecības pārstrādes atkritumus - skaidas ar dažādu mikroorganismu palīdzību: metāna rūgšanas baktērijas(Afe/Aanosarcina methanica sp-O-16,OG-3, Methanobacterium sp. A-5, Methanococcus sp.-D-l, Methanotrix soehngenii K-2), celulozes skaldītājas baktērijas: (Trichoderma viride -LL-333 un Trichoderma lignorum LL- 751) un slāpekļa aprites veicinātājas baktērijas: (Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp.DN-3 un Sarcina sp. 15 13-N) kompostēšanas laukumā stirpās.1. The invention relates to the protection of the environment and to agriculture, in particular to the development of a technology for the extraction of organic fertilizers by processing waste from dairy (unused sponges) and forestry by means of various microorganisms: methane fermentation bacteria (Afe / Aanosarcina methanica sp-O-16). , OG-3, Methanobacterium sp-A-5, Methanococcus sp.-Dl, Methanotrix soehngenii K-2), cellulose degrading bacteria: (Trichoderma viride -LL-333 and Trichoderma lignorum LL-751) and nitrogen-promoting bacteria: ( Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp.DN-3 and Sarcina sp.

Ir zināms komposta ieguves paņēmiens (LV 11798 no 07.04.1995. un LV 12107 no 20.08.1998.). Pēc minētajiem paņēmieniem kompostā izmanto organiskos atkritumus, kurus ievieto slēgtā konteinerā ar piespiedu ventilāciju. Izmantoto gaisu pilnīgi atgriež atpakaļ ciklā, ievadot to atpakaļ kompostējamā vielā bez jebkādas skābekļa papildus pievadīšanas slēgtajā konteinerā.A method of extracting compost is known (LV 11798 from 07.04.1995 and LV 12107 from 20.08.1998). According to these methods, compost uses organic waste, which is placed in a closed container with forced ventilation. Used air is completely returned to the cycle by being returned to the compostable material without any additional oxygen supply to the closed container.

Iepriekš minētā paņēmiena trūkums, pirmkārt ir tas, ka konteinera tilpums ir neliels un tā apjoms ierobežo kompostējamā materiāla daudzumu. Otrkārt, konteineram gaisu pievada ar piespiedu ventilāciju. Pie tādiem atkritumu apjomiem, kādi ir Lazdonas pienotavā un Praulienas pagasta mežos, nepieciešami būti milzīgi konteineri. Tas viss ārkārtīgi sadārdzinātu pašu kompostēšanas procesu. Nemaz jau nerunājot par ventilācijas iekārtas izmaksām, kas nepieciešamas gaisa pievadīšanai.The disadvantage of the above method is, first of all, that the volume of the container is small and its size limits the amount of compostable material. Second, the container is vented with forced ventilation. The amount of waste we have in Lazdona dairy and Prauliena Parish forests requires huge containers. All of this would make the composting process very expensive. Not to mention the cost of the air handling unit needed to supply air.

2. Piedāvātā izgudrojuma mērķis ir nepiesārņot apkārtējo vidi ar organiskas izcelsmes atkritumiem (neizmantotām sūkaliņām un zāģu skaidām), kurus iespējams mikrobioloģiski pārvērst lauksaimniecībai noderīgā kompostā.2. The object of the present invention is to avoid contamination of the environment with organic waste (unused sponges and sawdust) which can be microbiologically converted into agricultural compost.

3. Šo mērķi sasniedz tādejādi, ka minētos organiskos atkritumus kopā ar kaļķiem (vides pH reakcijas neitralizēšanai) speciāli izveidotā kompostēšanas laukumā pakāpeniski liek 3 m platās un 3 m augstās stirpās, kam pievieno metāna rūgšanas baktērijas (Methanosarcina methanica sp.-O-16, OG-3,3. This objective is achieved by the gradual introduction, in a specially created composting area, of the said organic waste together with lime (to neutralize the pH of the environment) in a 3 m wide and 3 m high lichen, to which methane fermentation bacteria (Methanosarcina methanica sp.-O-16, OG-3,

-2Methanobacterium sp. A-5, Methanococcus sp.-D- 1, Methanotrix soehngenii K- 2). Stirpu garums nav ierobežots. Sākumā sūkaliņām jābūt pārākumā, lai varētu nodrošināt tajās augstu mitruma pakāpi (anaerobu mezoffli -termofilu režīmu).-2Methanobacterium sp. A-5, Methanococcus sp.-D-1, Methanotrix soehngenii K-2). The length of the hoops is not limited. Initially, the sponges should be superior to provide a high level of moisture (anaerobic mesophyll-thermophilic regime).

4. Pakāpeniski notiek temperatūras paaugstināšanās līdz 45 - 60° C. Apmēram pēc 7-10 dienām temperatūra pazemināšanās līdz 20 -24°C. Tad stirpas sajauc, pievieno celulozes skaldītājas (Trichoderma viride -LL-333 un Trichoderma lignorum LL- 751) un nitrificējošās mikroorganismu asociācijas (Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp.DN-3 unSarcina sp. -13-N).4. The temperature gradually increases to 45-60 ° C. After about 7-10 days the temperature drops to 20-24 ° C. The stirrups are then mixed, cellulose disruptors (Trichoderma viride -LL-333 and Trichoderma lignorum LL-751) and nitrifying microorganism associations (Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp.DN-3 and Sarcina sp.) Are added. -13-N).

5. Lai uzturētu komposta stirpās aerobus apstākļus, tajās ik pa 1,5 m no sāniem un uz virsmas ieliek meliorācijas perforētās caurules.5. In order to maintain aerobic conditions in the compost, the drainage perforated pipes shall be placed at 1.5 m from the sides and on the surface.

6. Kā “ieraugu” jauna organisko atkritumu kompostēšanas stirpu veidošanā metāna rūgšanas (Mathanosarcina methanica sp.-O-16, OG-3, Methanobacterium sp. A-5, Methanococcus sp.-D-1, Methanotrix soehngenii K- 2), celulozes skaldītāju (Trichoderma viride -LL-333 un Trichoderma lignorum LL- 751) un nitrificējošo (Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp.DN-3 un Sarcina sp. -13-N) baktēriju vietā var izmantot daļu no gatavā komposta.6. As a "starter" for the formation of new organic waste composting hoops by methane fermentation (Mathanosarcina methanica sp.-O-16, OG-3, Methanobacterium sp. A-5, Methanococcus sp.-D-1, Methanotrix soehngenii K-2), cellulose disruptors (Trichoderma viride -LL-333 and Trichoderma lignorum LL-751) and nitrifying bacteria (Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp.DN-3 and Sarcina sp. -13-N) part of the finished compost can be used instead.

60-62g 12 g 10 g60-62g 12g 10g

45-47g45-47g

72000 - 7700072000 - 77000

51600-5540051600-55400

6,0 - 6,2 (tendence skābt!)6.0 - 6.2 (tend to sour!)

1000 kg 2000 kg 20 kg1000 kg 2000 kg 20 kg

Paņēmiens organisko atkritumu biokompostēšanai atklātā laukāMethod of biocomposting organic waste in open field

PiemērsExample

Sūkaliņu sastāvs (1):Composition of the sponges (1):

SausnaDry matter

TaukiFat

Olbaltumvielas Laktoze ĶSP, mg/1 BSPp, mg/1 PHProtein Lactose COD, mg / 1 BSPp, mg / 1 PH

Lapu koku skaidas SūkaļiņasDeciduous wood chips

Krīts vai dzēstie kaļķi Metāna rūgšanas baktērijas:Chalk or slaked lime Methane fermentation bacteria:

(Methanosarcina methanica sp.-O-16, OG-3,(Methanosarcina methanica sp.-O-16, OG-3,

Methanobacterium sp. A-5, Methanococcus sp.-D-1,Methanobacterium sp. A-5, Methanococcus sp.-D-1,

Methanotrix soehngenii K-2), 10%Methanotrix soehngenii K-2), 10%

Celulozes skaldītājas baktērijas: (Trichoderma viride -LL-333 un Trichoderma lignorum LL-751) 10%Cellulose degrading bacteria: (Trichoderma viride -LL-333 and Trichoderma lignorum LL-751) 10%

Slāpekļa aprites veicinātājas baktērijas:Nitrogen promoting bacteria:

(Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2,(Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2,

Nitrobacter sp.DN-3 un Sarcina sp. -13-N) 10%.Nitrobacter sp.DN-3 and Sarcina sp. -13-N) 10%.

Uz noblīvēta vai nobetonēta pamata veido komposta stirpu: apmēram 3 m platumā, garumā, cik tas ir nepieciešams, sakrauj 10 - 15 cm biezā kārtā lapu koku skaidas, samitrina tās ar sūkaliņām, pievieno kaļķus (jākontrolē vides reakciju pH - tai jābūt robežās no 6,8 līdz 8,0) un visbeidzot pievieno metāna rūgšanas baktērijas: (Methanosarcina methanica sp.-O-16, OG-3, Methanobacterium sp. A-5, Methanococcus sp.- D -1, Methanotrix soehngenii K- 2).Forms compost hoops on a compacted or concreted basis: stacking deciduous wood chips about 3 m wide, 10 - 15 cm thick as needed, dampen them with sponges, add lime (pH of environmental reactions should be controlled - should be within 6 , 8 to 8.0) and finally, methane fermentation bacteria (Methanosarcina methanica sp. -O-16, OG-3, Methanobacterium sp. A-5, Methanococcus sp.-D-1, Methanotrix soehngenii K-2) are added.

Tad atkal visu atkārto līdz komposta stirpas augstums ir 1 m. Ļauj noritēt mikrobioloģiskajiem procesiem. Notiek temperatūras paaugstināšanās no 40 līdz 55- 60° C. Šajā laikā kaudzei jābūt stipri slapjai, jo norit anaerobie procesi. Kad temperatūra sāk pazemināties,Then repeat everything until the height of the compost hoop is 1 m. Allows microbiological processes to take place. The temperature rises from 40 to 55-60 ° C. During this time, the pile must be very wet due to anaerobic processes. When the temperature starts to drop,

-2notiek kaudzes sablīvēšanās, tad krauj atkal virsū jaunu kārtu visu dara kā iepriekš. Komposta stirpas turpina kraut līdz 3 m augstumam. Pēc tam, kad temperatūra komposta stirpā ir pazeminājusies, to kārtīgi samaisa, tādejādi pārtraucot anaerobo un nodrošinot aerobo režīmu. Vienlaicīgi kompostējamam materiālam pievieno celulozes skaldītājas (Trichoderma viride -LL-333 un Trichoderma lignorum LL- 751) un slāpekļa aprites veicinātājas (Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp. DN - 3 un Sarcina sp. -13 - N) baktērijas. Lai intensificētu nitrifikācijas procesus, jānodrošina komposta stirpas ar pietiekamu gaisa padevi, ievadot tajā no sāniem līdz kaudzes vidum un no virsmas ik pa 1,5 m perforētas meliorācijas caurules.-2 the pile is compacted, then the stack is back on top doing a new round as before. The compost hoops continue to stack up to a height of 3 m. After the temperature in the compost hoop has dropped, it is thoroughly mixed, thereby interrupting the anaerobic and aerobic regime. Cellulose disintegrators (Trichoderma viride -LL-333 and Trichoderma lignorum LL-751) and nitrogen promoters (Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp. DN-3 and Sarcina sp.) Are added simultaneously to the compostable material. -13 - N) bacteria. To intensify nitrification processes, compost hoops must be provided with sufficient air supply through a perforated drainage pipe 1.5 m per side from the center to the center of the stack.

Sākumā oglekļa un slāpekļa attiecība kompostā ir 100:1, kad komposts gatavs - 20:1 vai 16:1.Initially, the ratio of carbon to nitrogen in compost is 100: 1, when the compost is ready: 20: 1 or 16: 1.

Pēc 6 mēnešiem analizē kompostējamo materiālu un nosaka: kopējo baktēriju skaitu, kopējo N un C saturu un to N/C, humīnskābes un fulvoskābes daudzumu un attiecības, vides reakciju pH. Biokompostam ir augsnei raksturīgā smarža, tas ir viegli birstošs, brūnā krāsā ar mitruma saturu robežās no 50 - 65 %. Bez tam biokompostam jāsatur slāpekli (N) ap 0,5- 0,8 %, fosforu (P) ap 0,4 0,7 %, kāliju (K2O) ap 0,15 - 0,35 %, vides reakcijai pH ir jābūt Robežās no pH 6,0 - 7,0. Kopējais mikroorganismu skaits 1 g biokomposta sausnas ir no 107 -108, bet nitrifikātoru šūnu skaits 60 ĪO’-IO11.After 6 months, the compostable material is analyzed and determined for: total bacterial count, total N and C content and their N / C, amount and ratio of humic acid and fulvic acid, pH of environmental reactions. Biocompost has a soil-like odor, is a loose, brown color with a moisture content of 50-65%. In addition, the biocompost should contain nitrogen (N) about 0.5-0.8%, phosphorus (P) about 0.4 0.7%, potassium (K 2 O) about 0.15-0.35%, pH of the environmental reaction should be in the range of pH 6.0 - 7.0. The total number of microorganisms per g of biocompost dry matter is from 10 7 to 10 8 and the number of nitrifying cells is 60 IO'-IO 11 .

Paņēmiens organisko atkritumu biokompostēšanai atklātā laukāA method of biocomposting organic waste in the open

Claims (6)

IZGUDROJUMA FORMULAINVENTION FORMULA 1. Organisko vielu: neizmantoto sūkaliņu un lapu koku zāģu skaidu kompostēšanas paņēmiens atklātā laukā stirpās, pielietojot specifiskas mikroorganismu asociācijas: metāna rūgšanas baktērijas (Methanosarcina methanica sp.-O-16, OG-3, Methanobacterium sp. A-5, Methanococcus sp.-D-1, Methanotrix soehngenii K- 2), celulozes skaldītājas baktērijas (Trichoderma viride -LL-333 un Trichoderma lignorum LL- 751) un slāpekļa aprites veicinātājas baktērijas (Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp.DN-3 un Sarcina sp. -13-N) un nodrošinot procesā kā anaerobu, tā aerobu režīmu.1. Method of composting organic matter: unused sponges and deciduous sawdust in open fields using specific microorganism associations: methane fermentation bacteria (Methanosarcina methanica sp.-O-16, OG-3, Methanobacterium sp. A-5, Methanococcus sp. -D-1, Methanotrix soehngenii K-2), cellulose-degrading bacteria (Trichoderma viride -LL-333 and Trichoderma lignorum LL-751) and nitrogen-promoting bacteria (Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2), Nitrobacter sp.DN-3 and Sarcina sp. -13-N) and providing both anaerobic and aerobic treatment in the process. 2. Paņēmiens saskaņā ar 1. punktu atšķiras ar to, ka kompostēšanā notiek vispirms anaerobais ar temperatūras paaugstināšanos līdz 55 -60°C un tam seko aerobais process.2. The process according to claim 1, wherein the composting is first anaerobic with a temperature rise of 55 to 60 ° C and then followed by an aerobic process. 3. Paņēmiens saskaņā ar 1. vai 2. punktu a t š ķ i r a s ar to, ka anaerobo procesu, kas ilgst 5-10 dienas pie 55 -60°C temperatūras, nodrošina paaugstinātais mitrums kompostā.3. A process according to claim 1 or 2, wherein the anaerobic process, which lasts for 5-10 days at 55-60 ° C, is provided by the increased moisture in the compost. 4. Paņēmiens saskaņā ar jebkuru 1.-3. punktu a t š ķ i r a s ar to, ka anaerobā procesa rezultātā kompostējamā materiālā aiziet bojā nevēlamā (nosacīti -patogēnā) mikroflora.A process according to any one of claims 1-3. A c h a r a c t e r i z e d in that anaerobic process results in the destruction of undesirable (relatively pathogenic) microflora in the material to be composted. 5. Paņēmiens saskaņā ar 4. punktu a t š ķ i r a s ar to, ka, lai paātrinātu kompostēšanas procesu un veicinātu augstas kvalitātes gala produkta ieguvi, pēc anaerobā procesa, kad temperatūra ir pazeminājusies, sajauc kompostējamo materiālu un pievieno celulozes skaldītājas baktērijas (Trichoderma viride -LL-333 un Trichoderma lignorum LL- 751) un slāpekļa aprites veicinātājasnitrifīcējošās baktērijas (Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp.DN-3 un Sarcina sp. -13-N).5. The method according to claim 4, wherein, in order to accelerate the composting process and to promote the production of a high quality finished product, the compostable material is mixed after the anaerobic process when the temperature has fallen and Trichoderma viride is added. LL-333 and Trichoderma lignorum LL-751) and nitrogen-promoting nitrifying bacteria (Pseudomonas sp. -DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp.DN-3 and Sarcina sp. -13-N). 6. Paņēmiens saskaņā ar 5. punktu a t š ķ i r a s ar to, ka nepieciešamo gaisu kompostam aerobā režīma laikā nodrošina,6. The method of claim 5, wherein the air required for the compost is aerobically provided, -2ievadot stirpu sānos un virsmā ik pa 1,5 m perforētas caurules.- Introducing a hinged 1.5 mm perforated pipe on the sides and on the surface. 35 7. Paņēmiens saskaņā ar jebkuru 1.- 6. punktu atšķiras ar to, ka var izmantot daļu no gatavā komposta kā “ieraugu” jauna organisko atkritumu kompostēšanai metāna rūgšanas (Methanosarcina methanica sp.-O-16, OG-3, Methanobacterium sp. A-5, Methanococcus sp. - D -1,7. A process as claimed in any one of claims 1-6, wherein a portion of the finished compost can be used as a "starter" for the composting of new organic waste by methane fermentation (Methanosarcina methanica sp.-O-16, OG-3, Methanobacterium sp. .A-5, Methanococcus sp. - D -1, 40 Methanotrix soehngenii K- 2), celulozes skaldītāju (Trichoderma viride - LL - 333 un Trichoderma lignorum LL-751) un slāpekļa aprites veicinātāju - nitrificējošo (Pseudomonas sģ. -DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp. DN- 3 un Sarcina sp. -13 - N) baktēriju vietā.40 Methanotrix soehngenii K-2), cellulose disruptor (Trichoderma viride - LL - 333 and Trichoderma lignorum LL - 751) and nitric acid promoter - nitrifying (Pseudomonas s. -DN-1, Nitrosomonas sp. DN-2, Nitrobacter sp. - 3 and Sarcina sp. -13 - N).
LVP-01-175A 2001-12-19 2001-12-19 Method for biocomposting the organic waste in field conditions LV13022B (en)

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