LU503937B1 - Laying hen feed for improving egg quality and method thereof - Google Patents

Laying hen feed for improving egg quality and method thereof Download PDF

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Publication number
LU503937B1
LU503937B1 LU503937A LU503937A LU503937B1 LU 503937 B1 LU503937 B1 LU 503937B1 LU 503937 A LU503937 A LU 503937A LU 503937 A LU503937 A LU 503937A LU 503937 B1 LU503937 B1 LU 503937B1
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Luxembourg
Prior art keywords
carotene
feed
present disclosure
vitamin
laying
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LU503937A
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German (de)
Inventor
Keyan Zhang
Liuan Li
Xiaoxue Yu
Shunyi Qin
Qian Wang
Yaping Yu
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Univ Tianjin Agricultural
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Priority to LU503937A priority Critical patent/LU503937B1/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/70Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75Feeding-stuffs specially adapted for particular animals for birds for poultry
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/179Colouring agents, e.g. pigmenting or dyeing agents
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Birds (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Feed For Specific Animals (AREA)
  • Fodder In General (AREA)

Abstract

The present disclosure relates to a laying hen feed for improving egg quality and a method thereof. The laying hen feed comprises a basal diet and β-carotene, and the β-carotene is added to the basal diet at a dosage of 0.4~5 g/kg. The method uses the laying hen feed to feed laying hens for at least 10 days, preferably 10~30 days. The present disclosure also relates to use of β-carotene in improving egg quality. The present disclosure mainly studies the enhancement effect of β-carotene on egg yolk color and optimizes the specific application dose of β-carotene. Based on the metabolism and transformation effect of β-carotene in laying hens, the content of vitamin A in egg yolk is increased and egg yolk color is deepened, so that the nutritional value and economic value of eggs are both improved.

Description

BL-5653
LAYING HEN FEED FOR IMPROVING EGG QUALITY AND METHOD THEREOF >>>
TECHNICAL FIELD
[0001] The present disclosure relates to the technical field of production of livestock and poultry products in animal husbandry, and in particular, to a laying hen feed for improving egg quality and a method thereof.
BACKGROUND
[0002] Vitamin A is an essential fat-soluble vitamin, which plays a vital role in maintaining normal physiological functions. Vitamin A not only has effects on vision, bone, epithelial tissue, growth and reproduction, immune function and pigment metabolism, but also has many biological functions such as inhibiting tumor growth, preventing obesity, resisting aging, maintaining inner ear auditory function and preventing senile dementia. In the world, vitamin A is regarded as an important nutrient that the body must take in. Therefore, appropriate intake of substances containing vitamin A is important for the protection of human and animal health. At present, vitamin A is widely used in health food, medicine production, animal husbandry production and many other fields. Vitamin A is a kind of vitamin which is widely used in the international market with huge production and sales.
Vitamin A, vitamin C and vitamin E are acted as the three pillar products of vitamins which has a good development prospect. Since vitamin A is a fat-soluble vitamin, animal-derived vitamin A is easier to be absorbed in the diet than plant-derived vitamin A and vitamin A in synthetic forms.
Animal-derived foods rich in vitamin A include cod liver oil, animal liver and egg. Egg is one of the best sources of nutrition for the human body. Every 100 grams of egg contains 12.8 g of protein, and the absorption rate of protein can be as high as 98%. Every 100 grams of egg contains 11-15 g of fat, which is also easy to be digested and absorbed by the body. Egg is also rich in lecithin, sterols, lecithin, calcium, phosphorus, iron, vitamin A and B vitamins. Therefore, vitamin A supplementation through egg intake is more efficient, convenient, and acceptable than vitamin A supplementation through oral tablets or liquid preparations.
[0003] Thus, it is particularly urgent to develop a way to improve egg quality and increase vitamin
A content in eggs, which has an important application value and a practical significance for improving the economic benefit of layer breeding and promoting the healthy development of poultry industry.
SUMMARY
[0004] In order to overcome at least one problem existing in the prior art, by means of feed nutrition regulation, egg yolk color can be deepened and vitamin A content in egg yolk can be 1
BL-5653 increased in the present disclosure based on the metabolism and transformation effect of B-carotene >’ in laying hens, thereby improving egg quality and economic value. Embodiments of the present disclosure provide a laying hen feed for improving egg quality and a method thereof, which improves the nutritional value and added value of eggs, and improves the economic benefits of laying hens.
[0005] Detailed technical solutions are as follows:
[0006] A first aspect of the present disclosure provides a laying hen feed for improving egg quality, which includes a basal diet and B-Carotene, wherein the B-carotene is added to the basal diet at a dosage of 0.4~5 g/kg.
[0007] Preferably, the B-carotene is added to the basal diet at a dosage of 0.5~4.5 g/kg.
[0008] A second aspect of the present disclosure provides a method for improving egg quality, which uses a laying hen feed to feed laying hens for at least 10 days, wherein the laying hen feed includes a basal diet and B-Carotene, and the B-carotene is added to the basal diet at a dosage of 0.4~5 g/kg.
[0009] Preferably, the method uses the laying hen feed to feed laying hens for 10~30 days, and the
B-carotene is added to the basal diet at a dosage of 0.5~4.5 g/kg.
[0010] Preferably, in the method, an improvement of the egg quality is to deepen egg yolk color.
[0011] Preferably, in the method, an improvement of the egg quality is to increase the content of vitamin A in egg yolk.
[0012] A third aspect of the present disclosure provides use of B-carotene in improving egg quality, wherein the B-carotene is added to a basal diet at a dosage of 0.4~5 g/kg to obtain a laying hen feed, and the laying hen feed is used to feed laying hens for at least 10 days.
[0013] Preferably, the B-carotene is added to the basal diet at a dosage of 0.5~4.5 g/kg to obtain the laying hen feed, and the laying hen feed is used to feed laying hens for 10~30 days.
[0014] Preferably, in the use, the B-carotene is used to deepen egg yolk color.
[0015] Preferably, in the use, the B-carotene is used to increase the content of vitamin A in egg yolk.
[0016] Preferably, the main difference between the vitamin A enriched eggs obtained in the above use and the ordinary eggs is that egg yolk color of the vitamin A enriched eggs is significantly darker than that of the ordinary eggs. In a specific example, one of the results showed that: egg yolk color index of the control group was 7.87, while egg yolk color index of the experimental group adding P-carotene was 12.80, which was significantly higher than that of the control group (P<0.05).
[0017] The above basal diet can be matched according to the NRC and the Chinese chicken feeding standards for the required nutrients of laying hens. The application doses of the above 2
BL-5653
B-carotene can be 0.5, 0.8, 1, 1.5, 3, 4, 4.5 g/kg, etc., and the above feeding days can be 10, 13 50.00% 25, 30, etc. More specifically, with 0.5 g/kg dosage of B-carotene, feeding time can be 10 or 15 days; or, with 1 g/kg dosage of B-carotene, feeding time can be 10 days; or, with 1.5 g/kg dosage of
B-carotene, feeding time can be 25 days; or, with 3 g/kg dosage of B-carotene, feeding time can be days; or, with 4 g/kg dosage of B-carotene, feeding time can be 20 days; or, with 4.5 g/kg dosage of B-carotene, feeding time can be 15 or 20 days.
[0018] In the above method and use, during the feeding of laying hens, lay hens can feed and drink freely, using conventional management and routine immunization. The feeding management method and feed preparation for laying hens can be determined according to the relevant Chinese national laws and regulations and feeding standards.
[0019] The present disclosure achieves the following beneficial effects:
[0020] The present disclosure mainly provides a method for improving egg quality, through focusing on the study of the effect of B-carotene on egg yolk color and optimizing the specific application dose of B-carotene. Based on the metabolism and transformation effect of B-carotene in laying hens, the content of vitamin À in egg yolk can be increased and egg yolk color can be deepened, thereby improving the nutritional value and economic value of eggs.
DETAILED DESCRIPTION
[0021] To make the objectives, technical solutions, and advantages of the present disclosure clearer, the present disclosure is described below with reference to the accompanying embodiments. It should be understood that the embodiments described herein are merely used to explain the present disclosure, rather than to limit the present disclosure. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present disclosure without creative efforts should fall within the protection scope of the present disclosure.
[0022] Apparently, the accompanying embodiments in the following description show merely some embodiments of the present disclosure, and a person of ordinary skill in the art may apply the present disclosure to other similar scenarios according to these embodiments without creative efforts. In addition, it can also be appreciated that, although it may take enduring and complex efforts to achieve such a development process, for those of ordinary skill in the art related to the present disclosure, some changes such as design, manufacturing or production made based on the technical content in the present disclosure are merely regular technical means, and should not be construed as insufficiency of the present disclosure.
[0023] The "embodiment" mentioned in the present disclosure means that a specific feature, structure, or characteristic described in combination with the embodiment may be included in at least one embodiment of the present disclosure. The phrase appearing in different parts of the 3
BL-5653 specification does not necessarily refer to the same embodiment or an independent or alternative. embodiment exclusive of other embodiments. It may be explicitly or implicitly appreciated by those of ordinary skill in the art that the embodiment described herein may be combined with other embodiments as long as no conflict occurs.
[0024] Unless otherwise defined, the technical or scientific terms used in the present disclosure are as they are usually understood by those of ordinary skill in the art to which the present disclosure pertains. The terms "one", "a", "the" and similar words are not meant to be limiting, and may represent a singular form or a plural form. The terms "include", "contain", "have" and any other variants in the present disclosure mean to cover the non-exclusive inclusion, for example, a process, method, system, product, or device that includes a series of steps or modules (units) is not necessarily limited to those steps or units which are clearly listed, but may include other steps or units which are not expressly listed or inherent to such a process, method, system, product, or device. "Connected", "interconnected", "coupled" and similar words in the present disclosure are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. The term "multiple" in the present disclosure means two or more. The term "and/or" describes associations between associated objects, and it indicates three types of relationships. For example, "A and/or B" may indicate that A exists alone, A and B coexist, or B exists alone. The character "/" generally indicates that the associated objects are in an "or" relationship. The terms "first", "second", "third" and so on in the present disclosure are intended to distinguish between similar objects but do not necessarily indicate a specific order of the objects.
[0025] Embodiment 1 - Screening of substances for deepening egg yolk color and optimization of its specific application dose
[0026] This embodiment determines a substance that can be used to deepen egg yolk color and also determines its specific application dose, which includes the following steps:
[0027] (1) The substance used to effectively deepen egg yolk color is determined. In this embodiment, the promoting effects of substances on egg yolk color deposition was investigated, in which the substances included chemical compound, plant or its Chinese herbal medicine composition such as P-carotene (beta-carotene), daidzein, capsaiculin, formononetin, quercetin, hesperidin, carrot, safflower, pumpkin and citrus peel. Considering the cost and effect, B-carotene was selected as the substance to deepen egg yolk color.
[0028] (2) The added amount of P-carotene in the feed (basal diet) is determined. In this embodiment, the application dose of B-carotene in the feed was screened according to Table 1.
According to the test results, it was found that when the added dose of B-carotene in the basal diet of laying hens was lower than 0.4 g/kg, it did not deepen the color of egg yolk. When the added dose of B-carotene in the basal diet of laying hens exceeded 5 g/kg, the laying rate and the health of 4
BL-5653 . LL. . . . LU503937 laying hens were affected, so the safe application dose of B-carotene in the basal diet of laying hens was less than 5 g/kg. Considering the effective dose and safe dose of B-carotene in the feed of laying hens, the dosage of B-carotene was determined to be 0.5-4.5 g/kg.
[0029] Table 1- Application dose screening of B-carotene in feed ... 6 | 020 00 . 8 | 050 0000
L. 9 | 080. 15 | 600 00
[0030] (3) The application time of B-carotene is determined. For the determination of feeding time, in this embodiment, it had investigated the change of yolk color of eggs by feeding B-carotene for 5, 10, 15, 20, 25 and 30 days, respectively. The results showed that the egg yolk color was significantly deepened when the feeding time was more than 10 days. Therefore, the minimum feeding time was determined to be 10 days. According to the above test results and considering the production cost, the feeding time of B-carotene was determined to be 10-30 days.
[0031] Based on the above experiment results, it was finally determined that the feeding method to improve egg quality was to feed laying hens with the basal diet added with 0.5-4.5 g/kg B-carotene for 10-30 days, and the optimal dosage and feeding time of different groups of B-carotene were showed in Table 2.
[0032] Table 2 - the optimal dosage and feeding time of different groups of B-carotene
Li | 1 | 8 | 3 | 4 |5 | 6)
B-carotene added dose (kg)
[0033] Embodiment 2 - Validation test I of method for improving egg quality
[0034] In this embodiment, laying hens in a free-range laying farm in Tianjin were fed ordinary feed added with B-carotene, and the quality of eggs produced by the laying hens had been verified.
The test comprises the following steps:
[0035] (1) Implement animals and groups
[0036] Ninety 42-week-old healthy Luhua laying hens were randomly divided into control group and experimental group with 3 replicates per group and 15 laying hens per replicate.
BL-5653
[0037] (2) Diet and feeding management 1000087
[0038] The laying hens in the control group were fed a basal diet, which is matched the nutrients of laying hens according to NRC and Chinese feeding standards. The laying hens in experimental group were fed a basal diet added with 0.5 g/kg B-carotene. The laying hens were raised in indoor flat land, and were free to eat and drink with routine immunization during the experiment. Each group was fed the corresponding experimental diet for 10 days after feeding the basal diet in pretest for 1 week.
[0039] (3) Sample collection and quality index measurement
[0040] On the 10th day of the experiment, 35 eggs were randomly selected from each group, of which 20 were used to detect the content of vitamin A in eggs and the remaining 15 were used to detect egg quality. The content of vitamin A in egg yolk was determined by High Performance
Liquid Chromatography (HPLC). The color of egg yolk was determined by Roche yolk color fan.
[0041] (4) Experimental effect
[0042] The experimental results were shown in Table 3. The experimental results showed that the content of vitamin A in egg yolk of laying hens in experimental group was significantly higher than that in control group (P<0.01). The yolk color index of the eggs produced by the laying hens in experimental group was significantly higher than that in control group (P<0.01).
[0043] Table 3 - effect of 0.5 g/kg B-carotene on egg yolk color and vitamin A content
Control group Experimental group
Vitamin A content 6.77720.456 10.856**+0.390 (ng/g)
Egg yolk color 7.867+0.307 10.533**+0.192
[0044] Note: * means significant difference from the control group (P<0.05), ** means extremely significant difference from the control group (P<0.01).
[0045] Embodiment 3 - Validation test II of method for improving egg quality
[0046] In this embodiment, laying hens in a laying farm in Tianjin were fed ordinary feed added with B-carotene, and the quality of eggs produced by the laying hens had been verified. The test comprises the following steps:
[0047] (1) Implement animals and groups
[0048] One hundred and twenty 40-week-old healthy Hy-line variety brown laying hens were randomly divided into control group and experimental group with 4 replicates per group and 15 laying hens per replicate.
[0049] (2) Diet and feeding management
[0050] The laying hens in the control group were fed a basal diet, which is matched the nutrients of laying hens according to NRC and Chinese feeding standards. The laying hens in experimental group were fed a basal diet added with 4.5 g/kg B-carotene. The laying hens were free to eat and 6
BL-5653 drink with routine immunization during the experiment. The test lasted for 20 days. 509957
[0051] (3) Sample collection and quality index measurement
[0052] On the 20th day of the experiment, 40 eggs were randomly selected from each group, of which 20 were used to detect vitamin A in eggs and the remaining 20 were used to detect egg quality. The content of vitamin A in egg yolk was determined by High Performance Liquid
Chromatography (HPLC). The color of egg yolk was determined by Roche yolk color fan.
[0053] (4) Experimental effect
[0054] The experimental results were shown in Table 4. The experimental results showed that the content of vitamin A in egg yolk of laying hens in experimental group was significantly higher than that in control group (P<0.01). The yolk color index of the eggs produced by the laying hens in experimental group was significantly higher than that in control group (P<0.01).
[0055] Table 4 - effect of 4.5 g/kg B-carotene on egg yolk color and vitamin A content
Control group Experimental group
Vitamin A content 5.204+0.308 16.366**+0.439 (ng/g)
Egg yolk color 7.33340.153 13.600**+0.163
[0056] Note: * means significant difference from the control group (P<0.05), ** means extremely significant difference from the control group (P<0.01).
[0057] According to the aforementioned embodiments, it can be known that the laying hen feed and the feeding method used in the present disclosure can effectively increase vitamin À content in the egg yolk and deepen the color of the egg yolk, thereby improving the egg quality and nutritional value.
[0058] The above embodiments are merely illustrative of several implementation manners of the present disclosure, and the description thereof is more specific and detailed, but is not to be construed as a limitation to the patentable scope of the present disclosure. It should be pointed out that several variations and improvements can be made by those of ordinary skill in the art without departing from the conception of the present disclosure, but such variations and improvements should fall within the protection scope of the present disclosure. Therefore, the protection scope of the present disclosure should be subject to the protection scope defined by the claims. 7

Claims (10)

BL-5653 LU503937 CLAIMS
1. A laying hen feed for improving egg quality, characterized in that the laying hen feed includes a basal diet and B-Carotene, wherein the B-carotene is added to the basal diet at a dosage of
0.4~5 g/kg.
2. The laying hen feed according to claim 1, wherein the B-carotene is added to the basal diet at a dosage of 0.5~4.5 g/kg.
3. A method for improving egg quality, characterized in that the method uses a laying hen feed to feed laying hens for at least 10 days; wherein the laying hen feed includes a basal diet and B-Carotene, and the B-carotene is added to the basal diet at a dosage of 0.4~5 g/kg.
4. The method according to claim 3, wherein the method uses the laying hen feed to feed laying hens for 10-30 days, and the B-carotene is added to the basal diet at a dosage of 0.5~4.5 g/kg.
5. The method according to claim 3, wherein an improvement of the egg quality is to deepen egg yolk color.
6. The method according to claim 3, wherein an improvement of the egg quality is to increase the content of vitamin A in egg yolk.
7. Use of B-carotene in improving egg quality, characterized in that the B-carotene is added to a basal diet at a dosage of 0.4~5 g/kg to obtain a laying hen feed, and the laying hen feed is used to feed laying hens for at least 10 days.
8. The use according to claim 7, wherein the B-carotene is added to the basal diet at a dosage of
0.5~4.5 g/kg to obtain the laying hen feed, and the laying hen feed is used to feed laying hens for 10~30 days.
9. The use according to claim 7, wherein the B-carotene is used to deepen egg yolk color.
10. The use according to claim 7, wherein the P-carotene is used to increase the content of vitamin A in egg yolk. 8
LU503937A 2023-04-14 2023-04-14 Laying hen feed for improving egg quality and method thereof LU503937B1 (en)

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Effective date: 20231016