LU501989B1 - Method for producing resvertrol-enriched eggs - Google Patents
Method for producing resvertrol-enriched eggs Download PDFInfo
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- LU501989B1 LU501989B1 LU501989A LU501989A LU501989B1 LU 501989 B1 LU501989 B1 LU 501989B1 LU 501989 A LU501989 A LU 501989A LU 501989 A LU501989 A LU 501989A LU 501989 B1 LU501989 B1 LU 501989B1
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- Prior art keywords
- resveratrol
- eggs
- enriched
- feed
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- 235000013601 eggs Nutrition 0.000 title claims abstract description 63
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 17
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 claims abstract description 85
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 claims abstract description 85
- 235000021283 resveratrol Nutrition 0.000 claims abstract description 85
- 229940016667 resveratrol Drugs 0.000 claims abstract description 85
- 239000003674 animal food additive Substances 0.000 claims abstract description 23
- 238000000034 method Methods 0.000 claims abstract description 20
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The present disclosure relates to a method for producing resveratrol-enriched eggs. The method utilizes the metabolism and transformation of resveratrol in laying hens and the promoting effect of ginkgo biloba and tea polyphenols on the metabolism and transformation of resveratrol. Specific feed additives, which is composed of resveratrol, ginkgo biloba and tea polyphenols in a weight ratio of 10-20: 50-70: 10-40, are added to a laying hen feed in an amount of 1%-2.5%, and laying hens are fed for 10-30 days to obtain resveratrol-enriched eggs. The method improves the nutritional value and added value of eggs. The eggs can exert the function of resveratrol contained in eggs, which can improve the antioxidant function of mice and can regulate the immune function of mice, while providing full-value nutrition. In addition, the method significantly enhances the nutritional value and economic value of eggs.
Description
BL-5485 ' LU501989 METHOD FOR PRODUCING RESVERTROL-ENRICHED EGGS CROSS REFERENCE TO RELATED APPLICATION(S)
[0001] This patent application claims the benefit and priority of Chinese Patent Application No. 2021104883761 filed on May 6, 2021, the disclosure of which is incorporated by reference herein in its entirety as part of the present application.
[0002] The present disclosure relates to the field of production of livestock and poultry products in animal husbandry, and in particular, to a method for producing resveratrol- enriched eggs.
[0003] Resveratrol is a non-flavonoid polyphenolic compound that is widely found in a variety of plants in nature. Studies have found that resveratrol has bioactive functions such as antibacterial, anti-inflammatory, anti-tumor, antioxidant and free radical scavenging. In addition, resveratrol also plays an important role in relieving heat stress, regulating energy and lipid metabolism, regulating intestinal environment, regulating immune function, and improving animal production performance. Therefore, proper intake of resveratrol is of great significance to safeguard human and animal health. At present, resveratrol has been used in many fields such as health food, cosmetics and animal husbandry production, and has a good development prospect.
[0004] Eggs are one of the best sources of nutrition. Each hectogram of eggs contains
12.8g of protein, and its protein digestion and absorption rate can be as high as 98%. Each hectogram of eggs contains 11-15g of fat which is also easily digested and absorbed by the body. In addition, eggs are also rich in lecithin, sterols, egg yolk, calcium, phosphorus, iron, vitamin A, vitamin D and B vitamins. Therefore, eggs are the “ideal nutrient reservoir”. However, under normal feeding conditions, eggs do not contain resveratrol (or its content is too low to be detected). Therefore, in production practice, it is particularly urgent to seek a simple and effective method for increasing the content of resveratrol in eggs, to achieve the purpose of improving the nutritional value of eggs and the added value of related products. Moreover, it has important application value and practical significance for improving the economic benefits of laying hens and promoting the
BL-5485 LU501989 healthy development of the poultry industry.
[0005] The present invention achieves the purpose of increasing the content of resveratrol in eggs by utilizing the metabolism and transformation effect of resveratrol in laying hens through the method of feed nutrition regulation. At present, there are no similar patents or research reports in the prior art.
[0006] Embodiments of the present disclosure provide a method for producing resveratrol-enriched eggs to improve the nutritional value and added value of eggs, so as to improve the economic benefits of laying hens.
[0007] Detailed technical solutions are as follows:
[0008] A first aspect of the present disclosure provides a method for producing resveratrol-enriched eggs, comprising: adding specific feed additives to a laying hen feed, wherein the specific feed additives is composed of resveratrol, ginkgo biloba and tea polyphenols in a weight ratio of 10-20: 50-70: 10-40; and, the addition amount of the specific feed additives is 1%-2.5% of the weight of the laying hen feed. The method used is as follows: adding specific feed additives to the ordinary feed of laying hens. As a limitation, the amount of specific feed additives added to the ordinary feed of laying hens is 1%-2.5% of the weight of the ordinary feed of laying hens. As a further limitation, the feeding process is that laying hens are fed the laying hen feed with 1%-2.5% specific feed additives for 10-30 days, wherein ad libitum to eat and drink, routine management and immunization are implemented during the feeding process. The present invention proposes that the feeding and management method of laying hens and the preparation of feed can be determined according to relevant national regulations and feeding standards.
[0009] A second aspect of the present disclosure provides use of the resveratrol-enriched eggs in effectively improving immunity, wherein the resveratrol-enriched eggs are obtained by the method described in the first aspect of the present disclosure. The experimental results showed that resveratrol-enriched eggs can effectively improve the antioxidant capacity of mice and have a certain regulatory effect on the immune function of mice.
[0010] The beneficial effect of the method for producing the resveratrol-enriched eggs disclosed in the present invention is as follows:
[0011] Utilizing the metabolism and transformation of resveratrol in laying hens, and
BL-5485 ’ LU501989 utilizing the promoting effect of ginkgo biloba and tea polyphenols on the metabolic transformation of resveratrol, the content of resveratrol can be increased in eggs to obtain functional eggs rich in resveratrol, which improves the nutritional value and added value of eggs. In addition, the eggs can also exert the function of resveratrol contained in the eggs while providing full-value nutrition, which significantly enhances the nutritional value and economic value of eggs.
[0012] The main difference between the resveratrol-enriched eggs obtained by the present invention and the common eggs is that the composition contains higher resveratrol content. Resveratrol is one of the components of resveratrol-enriched eggs, which is present inside the eggs. That is, people supplement resveratrol while eating eggs, which is much more convenient than eating ordinary eggs and products containing resveratrol separately. More importantly, supplementing resveratrol by eating eggs is easier for people to accept than supplementing resveratrol alone. It is generally believed that the nutrients deposited in livestock and poultry products after metabolism by the animal are safer and more acceptable to people than the same substances extracted or chemically synthesized.
[0013] The resveratrol-enriched eggs obtained by the present invention are fed to mice for testing. It is found that, compared with the control group fed with ordinary eggs, it significantly improve the total antioxidant capacity (T-AOC), superoxidate dismutase (SOD) activity and catalase (CAT) activity of the mouse liver, and can also significantly reduce plasma TNF-a in mice (see Table. 1). It is indicated that the resveratrol-enriched eggs obtained by the present invention can effectively improve the antioxidant capacity of mice and have a certain regulating effect on the immune function of mice.
[0014] Table. 1 Effects of resveratrol-enriched eggs on antioxidant capacity and tumor necrosis factor a in mice CT em Group prot prot mmol/g pg/ml
3.95 +0.24 1.72 +0.08 2.85 +0.30 27.61 +522 group
[0015] Note: * indicates a significant difference compared with the control group (P<
0.05); ** indicates a significant difference compared with the control group (P<0.01).
BL-5485 LU501989
[0016] The present invention mainly solves the method for producing resveratrol- enriched eggs. The effects of the effective amount of resveratrol added in the feed, the screening of substances that can promote the metabolism and transformation of resveratrol and the effect of feeding time on the content of resveratrol in eggs were investigated. The main difficulty lies in the determination of the effective addition amount of resveratrol (safe addition amount), the screening process of substances that promote the metabolic transformation of resveratrol and the determination of feeding time, which are the most important creative research contents in the present invention. Therefore, we have carried out the following experiments and studies.
[0017] Firstly, the effective dose (safe dose) range of resveratrol in laying hen feed 1s determined. We investigated the effect of resveratrol content in egg yolk and egg white by adding different concentrations (0.5 g/kg, 1.0 g/kg, 1.5 g/kg, 3.0 g/kg, 6.0 g/kg and 9.0 g/kg) of resveratrol to the feed for 10 days (the content of resveratrol in egg yolk and egg white was determined by high performance liquid chromatography). It was found that when the dosage of resveratrol in laying hen feed was lower than 1 g/kg, it did not show an increase in the content of resveratrol in egg yolk (see Table. 2). When the dosage of resveratrol in laying hen feed exceeds 6 g/kg, it would affect egg production rate of laying hens and the health of laying hens. Therefore, it is finally determined that the effective dose (safe dose) of resveratrol in laying hen feed ranges from 1.0-6.0 g/kg.
[0018] Table. 2 Effects of different concentrations of resveratrol in feed on resveratrol content in eggs
[0019] Note: Different lowercase letters on the shoulder indicate significant differences between groups (P<0.01).
[0020] Regarding the determination of feeding time, we investigated the effects of resveratrol concentrations of 0.5 g/kg, 1.0 g/kg, 1.5 g/kg, 3.0 g/kg, 6.0 g/kg and 9.0 g/kg
BL-5485 LU501989 on the resveratrol content of eggs for 10 days, 20 days and 30 days. Considering that the lower limit of the effective dose determined above is the added amount of 1.0 g/kg resveratrol, the feeding time is determined to be 10-30days.
[0021] Secondly, the transformation promoters are screened. The promoting effect of the 5 single-component on the deposition and transformation of resveratrol in eggs was investigated, in which the single-component includes compounds such as daidzein, formaononetin, biochanin, quercetin, puerarin, tea polyphenols, hesperidin and ginkgoin, as well as plant ingredients or traditional Chinese medicine ingredients such as red clover, pueraria, ginkgo biloba, sea buckthorn and citrus peel,. Taking into account the cost and effect, ginkgo biloba and tea polyphenols were screened out as transformation promoters.
[0022] Thirdly, the ratio of the three components in a particular additive was determined. According to Table. 3, the proportions of three components were screened, and the proportions were determined to be that resveratrol 10-20% (w/w), ginkgo biloba 50-70% (w/w) and tea polyphenols 10-40% (w/w).
[0023] Table. 3 Proportion screening of components in additives 2 | 10 | 30 | 40 | so | 60 | so | 40 | 6 | 30 | 30 | 40 | 50 | 40 | 30 2 | 7 | 5 leo | 0 | 15 | 25 | 35) 8 | 10 | 60 | 70 | 80 10 | 20 | 30 9 | 15 leo sols | 15| os | 10 | 20 Jeo |70 | 80 0 | o 0 11 | 5 leo slo oo] | 0 Jeol sol o | oo]
[0024] Finally, the amount of specific additives to be added to the feed was determined. According to the effective dose range of resveratrol in laying hen feed and the ratio relationship of three components, comprehensively considering cost factors, the dosage of this additive is determined to be 1.0-2.5%.
BL-5485 LU501989
[0025] The composition of the specific feed additive for increasing the resveratrol content in eggs 1s finally determined to be resveratrol, ginkgo biloba and tea polyphenols in a weight ratio of 10-20: 50-70: 10-40. In addition, the amount of the additive in the feed is 1%-2.5%.
[0026] To make the objectives, technical solutions, and advantages of the present disclosure clearer, the present disclosure is described below with reference to the embodiments. It should be understood that the embodiments described herein are merely used to explain the present disclosure, rather than to limit the present disclosure. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present disclosure without creative efforts should fall within the protection scope of the present disclosure.
[0027] The "embodiment" mentioned in the present disclosure means that a specific feature, structure, or characteristic described in combination with the embodiment may be included in at least one embodiment of the present disclosure. The phrase appearing in different parts of the specification does not necessarily refer to the same embodiment or an independent or alternative embodiment exclusive of other embodiments. It may be explicitly or implicitly appreciated by those of ordinary skill in the art that the embodiment described herein may be combined with other embodiments as long as no conflict occurs.
[0028] Unless otherwise defined, the technical or scientific terms used in the present disclosure are as they are usually understood by those of ordinary skill in the art to which the present disclosure pertains. The terms "one", "a", "the" and similar words are not meant to be limiting, and may represent a singular form or a plural form. The terms "include", "contain", "have" and any other variants in the present disclosure mean to cover the non-exclusive inclusion, for example, a process, method, system, product, or device that includes a series of steps or modules (units) is not necessarily limited to those steps or units which are clearly listed, but may include other steps or units which are not expressly listed or inherent to such a process, method, system, product, or device. "Connected", "interconnected", "coupled" and similar words in the present disclosure are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. The term "multiple" in the present disclosure
BL-5485 LU501989 means two or more. The term "and/or" describes associations between associated objects, and it indicates three types of relationships. For example, "A and/or B" may indicate that A exists alone, À and B coexist, or B exists alone. The character "/" generally indicates that the associated objects are in an "or" relationship. The terms "first", "second", "third" and so on in the present disclosure are intended to distinguish between similar objects but do not necessarily indicate a specific order of the objects.
[0029] The raw materials and reagents used in the present invention are all commercially available, such as resveratrol, ginkgo biloba, tea polyphenols, etc.
[0030] Embodiments 1-6
[0031] A method for producing resveratrol-enriched eggs
[0032] Embodiments 1-6 are the methods for producing resveratrol-enriched eggs respectively. The methods are all adding the specific feed additive into the laying hen feed to feed the laying hens. The difference between the methods is the composition ratio and the addition amount of the specific feed additive in each embodiment (Table. 4).
[0033] Table. 4 The composition ratio and the addition amount of the specific feed additive in Embodiments 1-6 Embodiment _ Ginkgo biloba (%) | 5055 | 60 64 67 | 70
[0034] Embodiment 7
[0035] Validation test of the method for producing resveratrol-enriched eggs
[0036] A free-range laying hen harm in Tianjin
[0037] (1) Implement animals and groups
[0038] 90 40-week-old healthy Luhua laying hens were selected and randomly divided into control group and experimental group, with 3 replicates in each group and 15 laying hens in each replicate.
[0039] (2) Diet and feeding management
[0040] The control group was fed the basal diet, and the nutrition required for laying hens was matched with NRC and the Chinese chicken breeding standards. The
BL-5485 LU501989 experimental group was fed the basal diet with 1.0% specific feed additive (the composition of the specific feed additive was resveratrol, ginkgo biloba and tea polyphenols in a weight ratio of 10:55:35). The laying hens in each group were reared indoors on flat ground. During the experiment, the laying hens had free access to food and water, and were routinely immunized. All groups were fed the corresponding experimental diet after fed the basal diet for 1 week, and the experimental period was 20 days.
[0041] (3) Sample collection and index detecting
[0042] At the 20th day of the experiment, 20 eggs were randomly selected from each group to detect the content of resveratrol in egg yolk and egg white. The content of resveratrol in egg yolk and egg white was determined by high performance liquid chromatography (HPLC). The pretreatment of the sample was as follows: weigh 1g of sample into a 50 mL brown centrifuge tube, add methanol to make up to 10 mL, mix well, vortex for 2 min, sonicate for 30 min, centrifuge at S000 r/min for 10 min, and filter the supernatant through a 0.22 um microporous membrane for later use. The chromatographic conditions for sample determination were as follows: liquid chromatographic column: C18 (5 um, 250mmx4.6mm); column temperature: 30°C; mobile phase: A: water + acetic acid, B: acetonitrile; flow rate: 1 mL/min; UV detection wavelength: 306nm; and, injection volume: 10 pL.
[0043] (4) Effect of Experiment
[0044] The test results are shown in Table. 5 and Table. 6. The test results showed that the content of resveratrol in the egg yolk of the experimental group was significantly higher than that of the control group (P<0.01) at the 10th and 20th day, and the content of resveratrol in the egg white of the test eggs was not detected.
[0045] Table. 5 Effect of 1% specific feed additive on the content of resveratrol in egg olk (ng/g Control group | Experimenlgonp |
[0046] Note: * indicates a significant difference compared with the control group (P<
0.05); ** indicates a very significant difference compared with the control group (P<
0.01); 0.00+0.00 indicates not detected, the same below.
BL-5485 LU501989
[0047] Table. 6 Effect of 1% specific feed additive on the content of resveratrol in egg white (ug/g Control group | Experimenlgonp |
[0048] Embodiment 8
[0049] Validation test of the method for producing resveratrol-enriched eggs
[0050] A laying hen harm in Tianjin
[0051] (1) Implement animals and groups
[0052] 256 30-week-old healthy Hailan brown-shell laying hens were selected and randomly divided into control group and experimental group, with 4 replicates in each group and 32 laying hens in each replicate.
[0053] (2) Diet and feeding management
[0054] The control group was fed the basal diet, and the nutrition required for laying hens was matched with NRC and the Chinese chicken breeding standards. The experimental group was fed the basal diet with 2.5% specific feed additive (the composition of the specific feed additive was resveratrol, ginkgo biloba and tea polyphenols in a weight ratio of 20:65:15). The laying hens in each group were reared indoors on flat ground. During the experiment, the laying hens had free access to food and water, and were routinely immunized. All groups were fed the corresponding experimental diet after fed the basal diet for 1 week, and the experimental period was 30 days.
[0055] (3) Sample collection and index detecting
[0056] At the 30th day of the experiment, 40 eggs were randomly selected from each group to detect the content of resveratrol in egg yolk and egg white. The content of resveratrol in egg yolk and egg white was determined by high performance liquid chromatography (HPLC). The pretreatment of the sample was as follows: weigh 1g of sample into a 50 mL brown centrifuge tube, add methanol to make up to 10 mL, mix well, vortex for 2 min, sonicate for 30 min, centrifuge at S000 r/min for 10 min, and filter the supernatant through a 0.22 um microporous membrane for later use. The chromatographic conditions for sample determination were as follows: liquid chromatographic column: C18 (5 um, 250mmx4.6mm); column temperature: 30°C; mobile phase: A: water + acetic acid, B: acetonitrile; flow rate: 1 mL/min; UV detection wavelength: 306nm; and,
BL-5485 LU501989 injection volume: 10 pL.
[0057] (4) Effect of Experiment
[0058] The test results are shown in Table. 7 and Table. 8. The test results showed that the content of resveratrol in the egg yolk of the experimental group was significantly higher than that of the control group (P<0.01) at the 20th and 30th day, and the content of resveratrol in the egg white of the test eggs was not detected.
[0059] Table. 7 Effect of 2.5% specific feed additive on the content of resveratrol in egg olk (ng/g LU |Controtgroup | Experimentalgroup |
[0060] Table. 8 Effect of 2.5% specific feed additive on the content of resveratrol in egg white (ug/g) _.. | Control group | Experimental group |
[0061] Conclusion: the present invention can effectively increase the content of resveratrol in eggs and can produce resveratrol-enriched eggs.
[0062] A person skilled in the art should understand that, the technical features of the above embodiments can be arbitrarily combined. In an effort to provide a concise description, not all possible combinations of all the technical features of the embodiments are described. However, these combinations of technical features should be construed as disclosed in the description as long as no contradiction occurs.
[0063] The above embodiments are merely illustrative of several implementation manners of the present disclosure, and the description thereof is more specific and detailed, but is not to be construed as a limitation to the patentable scope of the present disclosure. It should be pointed out that several variations and improvements can be made by those of ordinary skill in the art without departing from the conception of the present disclosure, but such variations and improvements should fall within the protection scope of the present disclosure. Therefore, the protection scope of the patent of the present disclosure should be subject to the appended claims.
Claims (3)
1. A method for producing resveratrol-enriched eggs, characterized in that the method comprising: adding specific feed additives to a laying hen feed; wherein the specific feed additives is composed of resveratrol, ginkgo biloba and tea polyphenols in a weight ratio of 10-20: 50-70: 10-40; and, the addition amount of the specific feed additives is 1%-2.5% of the weight of the laying hen feed.
2. The method according to claim 1, wherein laying hens are fed the laying hen feed with 1%-2.5% specific feed additives for 10-30 days.
3. Use of the resveratrol-enriched eggs obtained by the method of claim 1 in effectively improving immunity.
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