LU501274B1

LU501274B1 - Culture medium preparing system

Info

Publication number: LU501274B1
Application number: LU501274A
Authority: LU
Inventors: Siyang Zhou; Huajian Shang; Xiaofang Shen; Jiayun Qin; Haibo Zhang; Yurui Wu
Original assignee: Univ Hubei Arts & Science
Priority date: 2021-04-01
Filing date: 2021-12-03
Publication date: 2023-05-11
Also published as: CN113512482A; WO2022205992A1; LU501274A1

Abstract

Discloses is a culture medium preparing system including a feeding device, a mixing device and a dispensing device, the feeding device is formed with feeding channels, each of which is provided with a first feeding port and a second feeding port opposite to each other and for different materials to be introduced, a mixing chamber of the mixing device is provided with receiving ports communicated with second feeding ports for introducing the different materials into the mixing chamber, the mixing chamber is also provided with a mixing discharging port arranged at intervals from the receiving ports for leading out mixed materials, the dispensing device is formed with a dispensing channel for distributing the mixed materials and then leading out, and the dispensing channel is provided with a first dispensing port communicated with the mixing discharging port and a second dispensing port for communicating with a petri dish.

Description

CULTURE MEDIUM PREPARING SYSTEM 0501278

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is the nation stage of an International Application No.

PCT/CN2021/135389, which claims the priority of a Chinese patent application No. 202110359148 4, filed on April 1, 2021, and the titled "Culture Medium Preparing System".

TECHNICAL FIELD

[0002] This application relates to the technical field of preparing culture medium, in particular to a culture medium preparing system.

BACKGROUND

[0003] Biological culture medium usually contains coagulants such as agar and carrageenan.

It is not easy to dissolve at room temperature in the mixing process, and easy to form lumps.

Before being subpackaged, the traditional culture medium usually needs to be heated to dissolve the culture medium evenly before filling. All processing links are carried out step by step, and more operators are needed to operate in different processing links, furthermore the required cost of equipments is high, energy consumption is large, and the production efficiency is low, which does not meet the public demand of low carbon and environmental protection. In recent years, some people have put forward the stirring device of culture medium, but various operators are still needed to carry out other production processes. In the production process, the culture medium is easily exposed to external air, which leads to the pollution of the culture medium and the growth of other fungi or molds.

SUMMARY

[0004] The main objective of the present application is to provide a culture medium preparing system, aiming at realizing the integrated preparation of culture medium, thereby improving the efficiency of the preparation of culture medium and quality of culture medium.

[0005] In order to achieve the above objective, the present application provides a culture medium preparing system, which includes: a feeding device, wherein the feeding device is formed with a plurality of feeding channels, each of the feeding channels has a first feeding port and a second feeding port disposed opposite to each other, and a plurality of first feeding ports are respectively configured for different materials to be introduced;

a mixing device provided with a mixing chamber, wherein the mixing chamber is providéd/501274 with a plurality of receiving ports communicated with a plurality of second feeding ports, the plurality of receiving ports are configured to lead the different materials into the mixing chamber, the mixing chamber is also provided with a mixing discharging port arranged at intervals with the plurality of receiving ports and configured for leading out mixed materials; and a dispensing device provided with a dispensing channel, wherein the dispensing channel is configured for distributing the mixed materials and then leading the mixed materials out, the dispensing channel is provided with a first dispensing port and a second dispensing port which are arranged at intervals, the first dispensing port is communicated with the mixing dispensing port, and the second dispensing port is configured for communicating with a Petri dish.

[0006] In an embodiment, the feeding device includes a plurality of batching box structures, the plurality of batching box structures are respectively arranged corresponding to the plurality of feeding channels and communicated with the plurality of first feeding ports, and each of the batching box structures includes: a batching box body having an inner cavity configured to accommodate a material; a first weighing sensor arranged in the batching box body and configured to detect a weight of the material in the batching box body; and a first solenoid valve arranged between the batching box body and a corresponding second feeding port and communicated with the first weighing sensor.

In an embodiment, the feeding device includes a plurality of batching box structures, the plurality of batching box structures are respectively arranged corresponding to the plurality of feeding channels and communicated with the plurality of first feeding ports, and each of the feeding box structures includes a batching box body; a corresponding second feeding port is located at one end of the batching box body; each of the batching box structure further includes a first pushing assembly, the first pushing assembly includes: a first pushing plate arranged in the batching box body and movably installed along a length direction of the batching box body, wherein a first accommodating cavity far away from the corresponding second feeding port is defined between the first pushing plate and an inner wall surface of the batching box body; and a first air pipe having one end configured for communicating with an air pump, and another end communicated with the first accommodating cavity.

[0007] In one embodiment, an inner side wall of the first accommodating cavity is provided with a first exhaust port;

the first pushing assembly further includes a first return spring arranged in the firs}501274 accommodating cavity, one end of the first return spring is connected with the first pushing plate, and another end of the first return spring is connected with the inner side wall of the first accommodating cavity opposite to the first pushing plate, and the first return spring is configured for driving the first pushing plate to return after air is exhausted through the first exhaust port.

[0008] In an embodiment, the mixing device further includes a stirring device located in the mixing chamber, the stirring device includes: a stirring paddle located at a bottom portion of the mixing chamber, and rotatable along an axis extending in an up-down direction; and a driving motor configured for driving and connect the stirring paddle.

[0009] In one embodiment, the mixing device further includes a mixing box body, an inner cavity of the mixing box body forms the mixing chamber, and a thickness direction of the mixing box body is along the up-down direction; the mixing discharging port is located at a lower end of the mixing box body;

The mixing device further includes a second pushing assembly, the second pushing assembly includes: a second pushing plate arranged in the mixing chamber and movably installed in the up-down direction, wherein a second accommodating cavity is defined between the second pushing plate and an upper wall surface of the mixing box; and a second air pipe having one end configured for communicating with an air pump, and another end communicating with the second accommodating cavity; and/or a lower wall surface of the mixing chamber is provided with a heating resistance wire configured for heating the mixed materials in the mixing chamber.

[0010] In an embodiment, the dispensing device includes a dispensing box structure located on the dispensing channel and communicating with the dispensing channel, the dispensing box structure includes: a dispensing box body having an inner cavity configured to accommodate the mixed materials; a second weighing sensor arranged in the dispensing box body and configured for detecting a weight of the mixed materials in the dispensing box body; and a second solenoid valve arranged between the dispensing box body and the second dispensing port and communicated with the second weighing sensor.

[0011] In one embodiment, the second dispensing port is located at one side of the dispensing box body;

the dispensing box structure further includes a third pushing assembly, the third pushidg/501274 assembly includes: a third pushing plate arranged in the dispensing box body and movably installed along a length direction of the dispensing box body, and a third accommodating cavity far away from the second dispensing port is defined between the third pushing plate and an inner wall surface of the dispensing box body; and a third air pipe having one end configured for communicating with an air pump, and another end communicating with the third accommodating cavity.

[0012] In one embodiment, an inner side wall of the third accommodating cavity is provided with a third exhaust port; the third pushing assembly further includes a third return spring arranged in the third accommodating cavity, one end of the third return spring is connected with the third pushing plate, and another end of the third return spring is connected with the inner side wall of the third accommodating cavity opposite to the third pushing plate, the third return spring is configured for driving the third pushing plate to return after air is exhausted through the third exhaust port.

[0013] In one embodiment, the mixing chamber is further provided with a temperature sensor configured to detect a temperature in the mixing chamber; and/or a filter screen is arranged between the first dispensing port and the second dispensing port.

[0014] In the technical solution of the present application, the feeding device is formed with a plurality of feeding channels, each of the feeding channels has a first feeding port and a second feeding port disposed opposite to each other Different materials including hot water and coagulants are introduced into the feeding device through the plurality of corresponding first feeding ports, and led out from the second feeding ports. The mixing chamber is provided with a plurality of receiving ports communicated with the plurality of second feeding ports. The different materials are introduced into the mixing chamber through the second feeding ports to be uniformly mixed. The mixing chamber is also provided with a mixing discharging port arranged at intervals from the plurality of receiving ports. The mixed materials are led out through the mixing discharging port. The dispensing channel is provided with a first dispensing port and a second dispensing port arranged at intervals. The first dispensing port is communicated with the mixing discharging port. The mixed materials are introduced into the dispensing device through the mixing discharging port. Finally, through the second dispensing port, the dispensing device leads a certain weight of the mixed materials into the Petri dish communicated with the second dispensing port, thereby realizing the integrated preparation of the culture medium without manual control in the whole process, improving the production efficiency and avoiding the pollution of the culture medium caused by manual operation. LU501274

BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly explain the embodiments of the present application or the 5 technical solution in the related art, the drawings required in the description of embodiments or related art will be briefly described below. It will be apparent that the drawings described below are only some embodiments of the present application, and other drawings may be obtained from the structure shown in these drawings without any creative effort by those of ordinary skill in the art.

[0016] Fig. 1 is a schematic diagram of a culture medium preparing system according to an embodiment of the present application.

[0017] Description of drawing numbers: 100 Culture medium preparing | 252 Second exhaust port

Cam TT

FT [ved emo ee

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[0018] The realization of the objectives, functional features and advantages of the present invention will be further explained with reference to the accompanying drawings in combination with the embodiment.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0019] A clear and complete description of the technical aspects of the embodiments of the present application will be given below in conjunction with the accompanying drawings in the embodiments of the present application, and it will be apparent that the described embodiments are only part of the embodiments of the present application, not all of them. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of the present application.

[0020] It should be noted that, if there is a directivity indication (such as up, down, left, right, front, back, etc.) in the embodiment of the present application, the directivity indication is only used to explain the relative positional relationship, motion situation, etc. between the components in a particular posture (as shown in the figures). If the specific posture is changed, the directivity indication is changed accordingly.

[0021] In addition, if there are descriptions such as "first", "second" and the like in embodiments of the present application, the descriptions of "first", "second" and the like are for descriptive purposes only, and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Thus, the features defined as "first", "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and/or" in the whole text includes three parallel schemes, taking "A and/or B" as an example, it includes scheme A, or scheme B, or both scheme A and B. In addition, the technical solutions in various embodiments can be combined with each other, but must be on the basis that the ordinary skill in the art can realize it. When a combination of technical solutions conflicts or cannot be realized, it should be considered that the combination of technical solutions does not exist and is not within the scope claimed by the present application.

[0022] Biological culture medium usually contains coagulants such as agar and carrageenan.

Before being subpackaged, the traditional culture medium usually needs to be heated to dissolve the culture medium evenly before filling. All processing links are carried out step by step, arkd/501274 more operators are needed to operate in different processing links, furthermore the required cost of equipments is high, energy consumption is large, and the production efficiency is low, which does not meet the public demand of low carbon and environmental protection. In recent years, some people have put forward the stirring device of culture medium, but various operators are still needed to carry out other production processes. In the production process, the culture medium is easily exposed to external air, which leads to the pollution of the culture medium and the growth of other fungi or molds.

[0023] In view of this, the present application provides a culture medium preparing system, which integrates a plurality of steps into one system and completes them to realize integration and avoid polluting culture medium in the preparing processing. Fig. 1 shows an embodiment of the culture medium preparing system provided by the present application,

[0024] Referring to Fig. 1, the culture medium preparing system 100 includes a feeding device, a mixing device and a dispensing device. The feeding device is formed with a plurality of feeding channels, each of which has a first feeding port 11 and a second feeding port 12 disposed opposite to each other. The plurality of first feeding ports 11 are configured for different materials to be introduced. The mixing device is provided with a mixing chamber, the mixing chamber is provided with a plurality of receiving ports 21 communicated with the plurality of second feeding ports 12, the plurality of receiving ports 21 are arranged to introduce different materials into the mixing chamber. The mixing chamber is also provided with a mixing discharging port 22 arranged at intervals from the plurality of receiving ports 21, the mixing discharging port 22 is configured to lead out mixed materials. The dispensing device is provided with a dispensing channel, the dispensing channel is configured to distribute the mixed materials and then lead the mixed materials out. The dispensing channel is provided with a first dispensing port 31 and a second dispensing port 32 which are arranged at intervals. The first dispensing port 31 is communicated with the mixing discharging port 22, and the second dispensing port 32 is arranged to communicate with a Petri dish a.

[0025] In the technical solution of the present application, the feeding device is formed with a plurality of feeding channels, each of the feeding channels has a first feeding port 11 and a second feeding port 12 disposed opposite to each other. Different materials including hot water (in this case a hot water tank 5 is connected to a mixing box body 2) and coagulants are introduced into the feeding device through the plurality of corresponding first feeding ports 11, and led out from the second feeding ports 12. The mixing chamber is provided with a plurality of receiving ports 21 communicated with the plurality of second feeding ports 12. The different materials are introduced into the mixing chamber through the second feeding ports 12 to b&501274 uniformly mixed. The mixing chamber is also provided with a mixing discharging port 22 arranged at intervals from the plurality of receiving ports 21. The mixed materials are led out through the mixing discharging port 22. The dispensing channel is provided with a first dispensing port 31 and a second dispensing port 32 arranged at intervals. The first dispensing port 31 is communicated with the mixing discharging port 22. The mixed materials are introduced into the dispensing device through the mixing discharging port 22. Finally, through the second dispensing port 32, the dispensing device leads a certain weight of the mixed materials into the Petri dish a communicated with the second dispensing port 32, thereby realizing the integrated preparation of the culture medium without manual control in the whole process, improving the production efficiency and avoiding the pollution of the culture medium caused by manual operation.

[0026] In this embodiment, each of the first feeding ports 12 is communicated with a feeding barrel 111. The feeding barrel 111 is arranged in an up-down direction, a radius of the feeding barrel 111 is gradually reduced from a top to a bottom. When batching the feeding device, different materials need to be put into the corresponding feeding barrels 111. A feeding valve is arranged between the first feeding port 11 and a batching box body 1 to separate the feeding barrel from the batching box body 1, so that one-time feeding can be realized, manual multiple feeding can be avoided, and manpower can be saved.

[0027] In this embodiment, referring to Fig. 1, in order to realize the automatic weight distribution of the feeding device, the feeding device includes a plurality of batching box structures, the plurality of batching box structures are arranged corresponding to the plurality of feeding channels, and communicated with the plurality of first feeding ports 11. Each of the batching box structures includes a batching box body 1 configured to accommodate a material introduced from the first feeding port 11. A first weighing sensor 13 is also arranged in the batching box body 1, a first solenoid valve 121 is arranged between the batching box body 1 and the second feeding port 12, the first solenoid valve 121 is in communication with the first weighing sensor 13. When the first weighing sensor 13 reaches a set value, the feeding valve is closed to stop feeding material to the batching box 1, and the first weighing sensor 13 controls the first solenoid valve 121 to open, so that a certain weight of material is led out from the second feeding port 12, and the weights of materials corresponding to the batching boxes 1 can be accurately controlled at the same time, the working efficiency is improved, and manpower is saved.

[0028] In this embodiment, referring to Fig. 1, in order to avoid material in the batching box body 1 not being exported, the batching device further includes a first pushing assembly. TH&501274 first pushing assembly includes a first pushing plate 151 arranged in the batching box body 1.

The first pushing plate 151 can be movably mounted along a length direction of the batching box body 1. A first accommodating cavity 15 is defined between inner wall surfaces of the batching box body 1 far away from the second feeding port 12 and the first pushing plate 151. The first accommodating cavity 15 is communicated with a first air pipe 153. The first air pipe 153 is in communication with an air pump. The feeding valve is closed, the first solenoid valve 121 is opened, the air pump inflates the first accommodating cavity 15 through the first air pipe 153.

Under the action of air pressure, a volume of the first accommodating cavity 15 gradually increases, thus, the first pushing plate 151 is driven to move toward one end close to the second feeding port 12. At this time, the volume of the first accommodating cavity 15 is gradually increased, and then all the material is pushed into the second feeding port 12. This arrangement prevents the material from remaining in the batching box 1, improves the proportioning accuracy of the culture medium, and prevents blockage at the same time.

[0029] The present application is not limited to any reset mode of the first pushing plate 151.

A negative pressure may be provided to the first accommodating cavity 15 by the air pump, so that the volume of the first accommodating cavity 15 is reduced under the air pressure, thereby driving the first pushing plate 151 to reset. In this embodiment, the first pushing assembly further includes a first return spring 1511 arranged in the first accommodating cavity 15, one end of the first return spring 1511 is connected with the first pushing plate 151, the other end is connected to an inner side wall of the first accommodating cavity 15 opposite to the first pushing plate 151.

The inner side wall of the first accommodating cavity 15 is provided with a first exhaust port 152.

After the first pushing plate 151 pushes the material into the second feeding port 12, the first pushing plate 151 drives the first return spring 1511 to make the first return spring 1511 in a stretched state. At this time, the first exhaust port 152 is opened, the air in the first accommodating cavity 15 is discharged, and the pressure in the first accommodating cavity 15 is balanced with the atmosphere, the first return spring 1511 drives the first pushing plate 151 to return. This arrangement has a simple structure and the cost is saved.

[0030] In order to ensure that different materials are mixed evenly, in this embodiment, referring to Fig. 1, the mixing device also includes a stirring device located in the mixing chamber. The stirring device includes a stirring paddle 23 arranged at a bottom portion of the mixing chamber, the stirring paddle 23 is rotatable along an axis extending in the up-down direction, and driven to rotate by a driving motor, so that when different materials are introduced into the mixing chamber from the corresponding plurality of receiving ports 21, the driving motor drives the stirring paddle 23 to rotate to mix the different materials evenly. LUS01274

[0031] In other embodiments, uniform mixing of the materials in the mixing chamber can be achieved by shaking the materials, which will not be described herein.

[0032] Further, the mixing device also includes a mixing box body 2, a thickness direction of the mixing box body 2 is the up-down direction. A lower end of the mixing box body 2 is provided with the mixing discharging port 22. The mixing device further includes a second pushing assembly. The second pushing assembly includes a second pushing plate 251 arranged in the mixing chamber. The second pushing plate 251 is movably mounted in the up-down direction in the mixing chamber. A second accommodating cavity 25 is defined between the second pushing plate 251 and an upper wall surface of the mixing box body 2. The second accommodating cavity 25 is communicated with the second air pipe 253. The second air pipe 253 communicates with an air pump. At this time, each of the first solenoid valves 121 is closed, the air pump inflates the second accommodating cavity 25 through the second air pipe 253, a volume of the second accommodating cavity 25 is gradually increased, so that the second pushing plate 251 is driven downward to reduce a volume of the mixing chamber, the air in the mixing chamber is compressed, and the air pressure is increased, a high-pressure environment is formed in the mixing chamber, which is helpful to eliminate miscellaneous bacteria and improve the quality of the culture medium.

[0033] In the proportions of the culture medium, gel is usually added. Because of the characteristics of the gel, it is easy to solidify the materials in the mixing process. For this, in this embodiment, a lower wall surface of the mixing chamber is provided with a heating resistance wire 26. In this way, the mixed materials can be heated, and kept at a certain temperature to prevent solidification, moreover, cooperating with the second pushing assembly, high temperature and high pressure can be achieved, and the mixed materials are disinfected at high temperature and high pressure, miscellaneous bacteria in the mixed material are eliminated, and the production quality of the culture medium is further improved.

[0034] The present application is not limited to any reset mode of the second pushing plate 251. A negative pressure may be provided to the second accommodating cavity 25 by the air pump, so that the volume of the second accommodating cavity 25 is reduced under the air pressure, thereby driving the second pushing plate 251 to reset. In this embodiment, an inner side wall of the second accommodating cavity 25 is further provided with a second exhaust port 252.

The second pushing plate 251 assembly also includes a second return spring 2511 arranged in the second accommodating cavity 25. A lower end of the second return spring 2511 is connected with the second pushing plate 251, an upper end of the second return spring 2511 is connected to the inner side wall of the second accommodating cavity 25 opposite to the second pushing plat&/501274 251. After the mixed materials are treated at high temperature and high pressure, the second exhaust port 252 is opened, and the air in the second accommodating cavity 25 is discharged. At this time, the second return spring 2511 in a stretched state returns back, and drives the second pushing plate 251 to return, thereby the mixing device can be operated normally. This arrangement has simple structure and cost saving.

[0035] In order to lead a certain weight of mixed materials into the Petri dish a, a dispensing box structure is arranged in the dispensing channel. The dispensing box structure includes a dispensing box body 3. An inner cavity of the dispensing box body 3 is configured for containing the mixed materials. A second weighing sensor 33 is arranged in the dispensing box body 3, a second solenoid valve 321 is arranged between the dispensing box body 3 and the second dispensing port 32 and communicated with the second weighing sensor 33. The mixed materials are introduced into the dispensing box body 3 through the first dispensing port 31. When the second weighing sensor 33 reaches a set value, the mixing discharging port 22 is closed, and the second weighing sensor 33 controls the second solenoid valve 321 to open, so that the certain weight of mixed materials is led out to the Petri dish a from the second dispensing port 32. This arrangement facilitates the operation and improves the production efficiency.

[0036] Further, in this embodiment, referring to Fig. 1, in order to introduce all the mixed materials in the dispensing box body 3 into the Petri dish a, the dispensing box structure also includes a third pushing assembly. The third pushing assembly includes a third pushing plate 341 arranged in the dispensing box body 3. The third pushing plate 341 can be movably installed along a length direction of the dispensing box body 3. A third accommodating cavity 34 is defined between an inner wall surface of the dispensing box body 3 far away from the second dispensing port 32 and the third pushing plate 341. The third accommodating cavity 34 is communicated with a third air pipe 343. The third air pipe 343 is in communication with an air pump. At this time, the mixing discharging port 22 is closed, and the second solenoid valve 321 is opened, the air pump inflates the third accommodating cavity 34 through the third air pipe 343.

A volume of the third accommodating cavity 34 is gradually enlarged, so that the third pushing plate 341 is driven to move toward one end close to the second dispensing port 32, and pushes all the materials into the second dispensing port 32. This arrangement prevents the materials from remaining in the dispensing box body 3, improves the proportioning accuracy of the culture medium, and prevents blockage at the same time.

[0037] The present application is not limited to any reset mode of the third pushing plate 341.

A negative pressure may be provided to the third accommodating cavity 34 by the air pump, so that the volume of the third accommodating cavity 34 is reduced under the air pressure, theretk}501274 driving the third pushing plate 341 to reset. In this embodiment, the third pushing assembly further includes a third return spring 3411 arranged in the third accommodating cavity 34, one end of the third return spring 3411 is connected with the third pushing plate 341, the other end is connected to an inner side wall of the third accommodating cavity 34 opposite to the third pushing plate 341. A third exhaust port 342 is provided on the inner side wall of the third accommodating cavity 34. After the third pushing plate 341 pushes the mixed materials into the second dispensing port 32, the third pushing plate 341 drives the third return spring 3411 to make the third return spring 3411 in a stretched state. At this time, the third exhaust port 342 is opened, the air in the third accommodating cavity 34 is discharged, and the pressure in the third accommodating cavity 34 is balanced with the atmosphere, the third return spring 3411 drives the third pushing plate 341 to return. This arrangement has a simple structure and the cost is saved.

[0038] The mixed materials also contain coagulant, in order to prevent the mixed materialS in the mixing chamber from solidifying, the mixing chamber is further provided with a temperature sensor 27, and the heating control of the heating resistance wire 26 is carried out according to the temperature information fed back by the temperature sensor 27, thereby avoiding the solidification of the mixed materials in the mixing chamber, and improving the production efficiency and saving time.

[0039] In order to improve the quality of the produced culture medium, a filter screen 6 is arranged between the first dispensing port 31 and the second dispensing port 32. When the mixed materials are poured into the dispensing box 3 from the mixing chamber, the filter screen 6 filters out the bulk mixed materials to improve the quality of the produced culture medium and prevent the bulk mixed materials from being contained in the culture medium.

[0040] In this embodiment, in order to save space, a plurality of first air pipes 153 and second air pipes 253 are communicated with the first air pump 41, and the third air pipes 343 are communicated with the second air pump 42, thereby reducing the number of equipments, saving cost and space.

[0041] It should be noted that either one of the above two related technical features can be adopted or both of the above two related technical features can be adopted, and the effect of adopting the both is better, which not only avoids the solidification of the mixed materials in the mixing chamber, but also can filter out the bulk mixed materials.

[0042] It should be noted that a control device is provided to automatically adjusts the electric driver through programs.

[0043] In this embodiment, a first limit switch 14 is arranged on an inner wall of each of th&}501274 batching box bodies 1, and the first limit switch 14 is arranged on a side of the second feeding port 12 close to the first pushing plate 151. A second limit switch 24 is arranged on an inner wall of the mixing box body 2, and the second limit switch 24 is arranged on a side of the mixing discharging port 22 close to the second pushing plate 251. A third limit switch 35 is arranged on an inner wall of the dispensing box body 3, the third limit switch 35 is arranged on a side of the second discharging port close to the third pushing plate 341. When the plurality of pushing plates move to push materials and move to the positions of the corresponding limit switches, the limit switches feeds back information and opens the corresponding exhaust ports, so as to prevent the return spring from failing due to stretching too much when the pushing plates drive the corresponding return springs to move, and realize accurately pushing the materials.

[0044] In this application, the control device is electrically connected with the first weighing sensor 13, the second weighing sensor 33, the temperature sensor 27, a driving motor, each limit switch and each air pump. Referring to Fig. 1, a method of preparing culture medium by means of a control device combining with the culture medium preparing system 100, the first weighing sensor 13, the second weighing sensor 33, the temperature sensor 27, the driving motor and the air pumps is as follows:

[0045] providing different materials.

[0046] In this embodiment, the different materials include hot water (here a hot water tank is communicated with the mixing box body 2) and coagulants.

[0047] Introducing the different materials into corresponding batching box bodies 1, and each of the batching boxes 1 introduce a certain weight of a material into the mixing box body 2.

[0048] In this embodiment, the feeding valve corresponding to each batching box body 1 is opened, the different materials are introduced into the batching box bodies 1 from the first feeding port 11 corresponding to each batching box body 1. Each of the first weighing sensors 13 has set a value corresponding to proportions in the culture medium to be prepared. When the first weighing sensor 13 reaches the set value, the first solenoid valve 121 is opened, and the first air pump 41 fills the first chamber 15 with high-pressure air, which drives the first pushing plate 151 to move until reaching the position of the first limit switch 14. At this time, all the materials in the batching box body 1 are introduced into the mixing chamber.

[0049] Mixing the different materials in the mixing chamber.

[0050] In this embodiment, the driving motor drives the stirring paddle 23 to rotate along the axis extending in the up-down direction, and the driving motor stops after the different materials are uniformly mixed.

[0051] Pressurizing the second accommodating cavity 25 by the air pump to move th&501274 second pushing plate 251 downward to compress the air in the mixing chamber, and at the same time heating the mixed materials by the heating resistance wire.

[0052] In this embodiment, the first solenoid valve 121 and the mixing discharging port 22 are closed. The first air pump 41 fills the second accommodating cavity 25 with air, and the second pushing plate 251 moves downward to compress the air in the mixing chamber, so that the pressure in the mixing chamber increases, and at the same time, the heating resistance wire heats the mixed materials, so that a high temperature and high pressure environment is formed in the mixing chamber, and the mixed materials are sterilized at high temperature.

[0053] Opening the second exhaust port 252 to reset the second pushing plate 251, and switching the heating resistance wire 26 to a heat preservation mode.

[0054] In this embodiment, after high-temperature sterilization of the mixed materials, the heating resistance wire 26 is switched to a heat preservation mode. When the temperature of the mixing materials is lowered to a set value T1 of the temperature sensor 27, the temperature sensor 27 controls the heating resistance wire 26 to heat the mixing materials. When the temperature rises to a set value T2 of the temperature sensor 27, the temperature sensor 27 controls the heating resistance wire 26 to stop heating, thereby maintaining the temperature of the mixing materials between the set value T1 and the set value T2. The set values can be changed according to the types of coagulants, which is not limited herein.

[0055] Opening the mixing discharging port 22, and leading the mixed materials into the dispensing box.

[0056] The dispensing box leads a certain weight of mixed materials into the petri dish;

[0057] In this embodiment, in the process of introducing the mixed materials, when the second weighing sensor 33 reaches a set value, the first air pump 41 inflates the third accommodating cavity 34, the third pushing plate 341 moves until it reaches the third limit switch 35, thus, a certain weight of the mixing materials is poured into the Petri dish a. After a delay of 5 seconds, the third exhaust port 342 is opened so that the third pushing plate 341 is reset. The 5 seconds here is only for the descriptive purpose of the present application, and the specific time is determined by the production of different weights of culture medium, which is not limited here.

[0058] In order to prevent blockage in parts of the dispensing passages communicated between the mixing box body 2 and the dispensing box body 3, the second solenoid valve 321 is closed, the first dispensing port 31 and the mixing discharging port 22 are opened. The second air pump 42 inflates the third accommodating cavity 34, and the third pushing plate 341 moves to compress the air in a dispensing chamber, so that the residual mixed materials in the parts b501274 the dispensing passages communicated between the mixing box body 2 and the dispensing box body 3 is pushed into the mixing chamber, and then the third exhaust port 342 is opened, and the third pushing plate 341 is reset.

[0059] The above are only preferred embodiments of the present application, and are not therefore limiting the patent scope of the present application. Any equivalent structure or equivalent process transformation made by using the contents of the specification and drawings of the present application, or any direct or indirect application in other related technical fields, under the application concept of the present application, are equally included in the scope of the present application.

Claims

CLAIMS LU501274

1. À culture medium preparing system comprising: a feeding device, wherein the feeding device 1s formed with a plurality of feeding channels, each of the feeding channels has a first feeding port and a second feeding port disposed opposite to each other, and a plurality of first feeding ports are respectively configured for different materials to be introduced; a mixing device provided with a mixing chamber, wherein the mixing chamber is provided with a plurality of receiving ports communicated with a plurality of second feeding ports, the plurality of receiving ports are configured to lead the different materials into the mixing chamber, the mixing chamber is also provided with a mixing discharging port arranged at intervals with the plurality of receiving ports and configured for leading out mixed materials; and a dispensing device provided with a dispensing channel, wherein the dispensing channel is configured for distributing the mixed materials and then leading the mixed materials out, the dispensing channel is provided with a first dispensing port and a second dispensing port which are arranged at intervals, the first dispensing port is communicated with the mixing dispensing port, and the second dispensing port is configured for communicating with a Petri dish.

2. The culture medium preparing system according to claim 1, wherein the feeding device comprises a plurality of batching box structures, the plurality of batching box structures are respectively arranged corresponding to the plurality of feeding channels and communicated with the plurality of first feeding ports, and each of the batching box structures comprises: a batching box body having an inner cavity configured to accommodate a material; a first weighing sensor arranged in the batching box body and configured to detect a weight of the material in the batching box body; and a first solenoid valve arranged between the batching box body and a corresponding second feeding port, wherein the first solenoid valve is communicated with the first weighing sensor, and is opened or closed by the first weighing sensor according to the weight of the material in the batching box body.

3. The culture medium preparing system according to claim 1, wherein the feeding device comprises a plurality of batching box structures, the plurality of batching box structures are respectively arranged corresponding to the plurality of feeding channels and communicated with the plurality of first feeding ports, and each of the feeding box structures comprises a batching box body; LU501274 a corresponding second feeding port 1s located at one end of the batching box body; each of the batching box structure further comprises a first pushing assembly, the first pushing assembly comprises: a first pushing plate arranged in the batching box body and movably installed along a length direction of the batching box body, wherein a first accommodating cavity far away from the corresponding second feeding port is defined between the first pushing plate and an inner wall surface of the batching box body; and a first air pipe having one end configured for communicating with an air pump, and another end communicated with the first accommodating cavity.

4. The culture medium preparing system according to claim 3, wherein an inner side wall of the first accommodating cavity is provided with a first exhaust port; the first pushing assembly further comprises a first return spring arranged in the first accommodating cavity, one end of the first return spring is connected with the first pushing plate, and another end of the first return spring is connected with the inner side wall of the first accommodating cavity opposite to the first pushing plate, and the first return spring is configured for driving the first pushing plate to return after air is exhausted through the first exhaust port.

5. The culture medium preparing system according to claim 1, wherein the mixing device further comprises a stirring device located in the mixing chamber, the stirring device comprises: a stirring paddle located at a bottom portion of the mixing chamber, and rotatable along an axis extending in an up-down direction; and a driving motor configured for driving and connect the stirring paddle.

6. The culture medium preparing system according to claim 1, wherein the mixing device further comprises a mixing box body, an inner cavity of the mixing box body forms the mixing chamber, and a thickness direction of the mixing box body is along the up-down direction; the mixing discharging port is located at a lower end of the mixing box body; The mixing device further comprises a second pushing assembly, the second pushing assembly comprises: a second pushing plate arranged in the mixing chamber and movably installed in the up-down direction, wherein a second accommodating cavity is defined between the second pushing plate and an upper wall surface of the mixing box; and a second air pipe having one end configured for communicating with an air pump, adhd/501274 another end communicating with the second accommodating cavity; and/or a lower wall surface of the mixing chamber is provided with a heating resistance wire configured for heating the mixed materials in the mixing chamber.

7. The culture medium preparing system according to claim 1, wherein the dispensing device comprises a dispensing box structure located on the dispensing channel and communicating with the dispensing channel, the dispensing box structure comprises: a dispensing box body having an inner cavity configured to accommodate the mixed materials; a second weighing sensor arranged in the dispensing box body and configured for detecting a weight of the mixed materials in the dispensing box body; and a second solenoid valve arranged between the dispensing box body and the second dispensing port, wherein the second solenoid valve is communicated with the second weighing sensor, and is opened or closed by the second weighing sensor according to a weight of the mixed materials in the dispensing box body.

8. The culture medium preparing system according to claim 7, wherein the second dispensing port is located at one side of the dispensing box body; the dispensing box structure further comprises a third pushing assembly, the third pushing assembly comprises: a third pushing plate arranged in the dispensing box body and movably installed along a length direction of the dispensing box body, and a third accommodating cavity far away from the second dispensing port is defined between the third pushing plate and an inner wall surface of the dispensing box body; and a third air pipe having one end configured for communicating with an air pump, and another end communicating with the third accommodating cavity.

9. The culture medium preparing system according to claim 8, wherein an inner side wall of the third accommodating cavity is provided with a third exhaust port; the third pushing assembly further comprises a third return spring arranged in the third accommodating cavity, one end of the third return spring is connected with the third pushing plate, and another end of the third return spring is connected with the inner side wall of the third accommodating cavity opposite to the third pushing plate, the third return spring is configured for driving the third pushing plate to return after air is exhausted through the third exhaust port. LU501274

10. The culture medium preparing system of claim 1, wherein the mixing chamber is further provided with a temperature sensor configured to detect a temperature in the mixing chamber; and/or a filter screen is arranged between the first dispensing port and the second dispensing port.