CN205443257U - Anaerobic culture medium prepares system - Google Patents
Anaerobic culture medium prepares system Download PDFInfo
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- CN205443257U CN205443257U CN201620127468.1U CN201620127468U CN205443257U CN 205443257 U CN205443257 U CN 205443257U CN 201620127468 U CN201620127468 U CN 201620127468U CN 205443257 U CN205443257 U CN 205443257U
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- oil
- reactor
- tank body
- culture medium
- anaerobic culture
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- 239000001963 growth medium Substances 0.000 title claims abstract description 46
- 238000010438 heat treatment Methods 0.000 claims abstract description 33
- 238000001816 cooling Methods 0.000 claims abstract description 18
- 238000003860 storage Methods 0.000 claims abstract description 17
- 238000003756 stirring Methods 0.000 claims abstract description 15
- 238000002360 preparation method Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 239000008213 purified water Substances 0.000 claims description 23
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 21
- 238000011177 media preparation Methods 0.000 claims description 16
- 229910052757 nitrogen Inorganic materials 0.000 claims description 10
- 239000003651 drinking water Substances 0.000 claims description 8
- 235000020188 drinking water Nutrition 0.000 claims description 8
- 239000002609 medium Substances 0.000 claims description 6
- 239000012092 media component Substances 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 239000000306 component Substances 0.000 abstract description 5
- 230000008878 coupling Effects 0.000 abstract 2
- 238000010168 coupling process Methods 0.000 abstract 2
- 238000005859 coupling reaction Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 238000009835 boiling Methods 0.000 description 3
- 239000003638 chemical reducing agent Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 238000006392 deoxygenation reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 230000003519 ventilatory effect Effects 0.000 description 1
Abstract
The utility model relates to an anaerobic culture medium prepares system. Including and carrying out a reation kettle of predissolve and stirring and the 2nd reation kettle that the preparation generated anaerobic culture medium to the culture medium component, a reation kettle's drain hole is connected to the 2nd reation kettle's feed inlet, be equipped with heating jacket on a reation kettle's the jar body, be equipped with heating jacket and cooling coil on the 2nd reation kettle's the jar body, still include the heat conduction oil furnace, cold oil storage tank and oil pump, the oil inlet of heat conduction oil furnace and cold oil storage tank oil -out between them and oil pump is through tee bend valve and tube coupling, two reation kettle's the heat oil export and the oil return opening of heat conduction oil furnace are through tee bend valve and tube coupling, the 2nd reation kettle's cold oil export is connected with the oil return opening of cold oil storage tank through the pipeline, the oil -out of oil pump is equipped with the tee bend valve, an exit linkage of this tee bend valve to the 2nd reation kettle's cold oil entry, another export is equipped with the tee bend valve, two exports of this tee bend valve are connected to two reation kettle's hot oil -in respectively.
Description
Technical field
This utility model belongs to anaerobic microorganism culturing device technical field, particularly relates to a kind of anaerobic culture medium preparation system.
Background technology
The method of present preparation anaerobic culture medium applies in general to laboratory and carries out small lot preparation, uses vacuumizing method or boils oxygen method of driving, when high-volume carries out anaerobic culture medium preparation, it is impossible to the anaerobic of guarantee anaerobic culture medium, and complex operation.
The compound method of anaerobic culture medium has vacuumizing method, boils and drive oxygen method at present.Vacuumizing method is the anaerobic culture medium prepared to be dispensed in small container (anaerobism pipe or triangular flask) seal, then by pipeline, vacuum pump is connected and evacuation with container, is then charged with oxygenless gas, 2-3 time the most repeatedly, sterilizing.Or being placed on after sterilizing in anaerobic box, anaerobic box carries out evacuation and is then charged with oxygenless gas, a period of time during then culture medium is placed on oxygen-free environment, form anaerobic environment.Boil and drive oxygen method, be that the culture medium prepared is contained in triangular flask, heated and boiled, it is passed through oxygenless gas simultaneously, keeps 15-30min, be cooled to room temperature under ventilatory conditions the most again, then carry out subpackage sterilizing.
Wherein vacuumizing method cannot be carried out high-volume and prepares anaerobic culture medium, and in anaerobic box, General Spatial is limited.Boiling method can cause moisture to reduce, higher than vacuumizing method in the work efficiency of preparation mass propgation base, but container is restricted, and affects efficiency.
Utility model content
This utility model provides a kind of anaerobic culture medium preparation system for solving technical problem present in known technology, can prepare anaerobic culture medium in high volume and ensure anaerobic, providing growing environment to anaerobe.
This utility model is adopted the technical scheme that by solving technical problem present in known technology: a kind of anaerobic culture medium preparation system includes nutrient media components carries out predissolve and the first reactor of stirring and the second reactor of preparation generation anaerobic culture medium, and the first drain hole of the first reactor is connected to the second charging aperture of the second reactor by pipeline；First tank body of the first reactor is provided with the first heating jacket with conduction oil as medium, and the second tank body of the second reactor is provided with the second heating jacket with conduction oil as medium and the cooling coil with cooling oil as medium；Also include heat-conducting oil furnace, cold oil storage tank and oil pump, the oil-in of heat-conducting oil furnace and the oil-out of cold oil storage tank and oil pump is connected by three-way valve and pipeline, first hot oil outlet of the first reactor, second hot oil outlet of the second reactor and the oil return opening of heat-conducting oil furnace are connected by three-way valve and pipeline, second cold oil outlet of the second reactor is connected by the oil return opening of pipeline with cold oil storage tank, the oil-out of oil pump is provided with three-way valve, one outlet of this three-way valve is connected to the second cold oil entrance of the second reactor by pipeline, another outlet is provided with three-way valve by pipeline, two outlets of this three-way valve are respectively connecting to the first deep fat entrance of the first reactor and the second deep fat entrance of the second reactor.
Advantage of the present utility model and good effect be: anaerobic culture medium preparation system of the present utility model has the advantage that, (1) can prepare anaerobic culture medium in high volume；(2) dividing two reactors to prepare, the first reactor ensures that medium component fully dissolves.Culture medium finally prepared by second reactor, during mixing can fast cooling, and with nitrogen protect, add thermo-labile component and reducing agent, the loss of nutritional labeling can be reduced；The circular disperser with holes of (3) second reactor nitrogen inlet end strips, can make gas Quick uniform be distributed to (4) second reactor band temperature-measuring ports in culture medium, dropping mouth, convenient mensuration temperature and regulation pH value；(5) second reactor band hand holes, visor, liquidometer, convenient operation；(6) clamping device can heat-conducting oil heating, culture medium can be heated, boil and deoxygenation；Can lower the temperature by cold water, make culture medium quickly cool down；(7) closed environment, band air inlet/outlet, with air in oxygenless gas displacement still, keep oxygen-free environment, it is ensured that the anaerobic of anaerobic culture medium, the growth for anaerobe provides favorable environment；(8) stainless steel, easy cleaning, without dead angle, anticorrosion are used；(9) inner pot pressure 0.1MPa, chuck pressure 0.2MPa, it is ensured that use safety.
Preferably: the first reactor includes the first tank body and the rabbling mechanism being made up of the first reductor and the first stirring paddle, and the first heating jacket is coated on the outside of the first tank body, and the first drain hole is positioned at the bottom of the first tank body；The roof of the first tank body is provided with the first charging aperture and the first purified water entrance.
Preferably: on the roof of the first tank body, be additionally provided with the first temperature-measuring port, the sidewall of the first heating jacket is additionally provided with the second temperature-measuring port.
Preferably: on the roof of the first tank body, be additionally provided with the first drinking water entrance.
Preferably: the second reactor includes the second tank body and the rabbling mechanism being made up of the second reductor and the second stirring paddle, and cooling coil is coated on the outside of the second tank body, and the second heating jacket is coated on the outside of cooling coil；Be provided with the second drain hole in the bottom of the second tank body, the second charging aperture is arranged on the roof of the second tank body；The roof of the second tank body is additionally provided with the second purified water entrance, nitrogen inlet and dropping mouth.
Preferably: on the roof of the second tank body, be additionally provided with the 3rd temperature-measuring port, the sidewall of the second heating jacket is additionally provided with the 4th temperature-measuring port.
Preferably: on the roof of the second tank body, be additionally provided with the second drinking water entrance and compressed air inlet.
Preferably: on the roof of the second tank body, be also equipped with mirror mouth.
Preferably: on the roof of the second tank body, be also equipped with hand hole.
Preferably: also include the measuring tank of purified water, the second purified water entrance of the outlet of measuring tank, the first purified water entrance of the first reactor and the second reactor uses three-way valve and pipeline to connect.
Accompanying drawing explanation
Fig. 1 is system structure schematic diagram of the present utility model；
Fig. 2 is the main TV structure schematic diagram of the first reactor in this utility model；
Fig. 3 is the plan structure schematic diagram of the first reactor in this utility model；
Fig. 4 is the main TV structure schematic diagram of the second reactor in this utility model；
Fig. 5 is the plan structure schematic diagram of the second reactor in this utility model.
In figure: 1, measuring tank；2, the first reactor；2-1, the first reductor；2-2, the first tank body；2-3, the first heating jacket；2-4, supporting leg；2-5, the first stirring slurry；2-6, the first hot oil outlet；2-7, the first drain hole；2-8, the first temperature-measuring port；2-9, the second temperature-measuring port；2-10, oil spilling groove；2-11, the first deep fat entrance；2-12, the first charging aperture；2-13, the first drinking water entrance；2-14, the first purified water entrance；3, the second reactor；3-1, the second reductor；3-2, the second tank body；3-3, the second heating jacket；3-4, supporting leg；3-5, the second stirring slurry；3-6, the second hot oil outlet；3-7, the second drain hole；3-8, cold oil entrance；3-9, hand hole；3-10, interface of the level gauge；3-11, the 3rd temperature-measuring port；3-12, air outlet slit；3-13, oil spilling groove；3-14, the 4th temperature-measuring port；3-15, the second deep fat entrance；3-16, cold oil export；3-17, nitrogen inlet；3-18, dropping mouth；3-19, the second drinking water entrance；3-20, the second purified water entrance；3-21, the second charging aperture；3-22, compressed air inlet；3-23, mirror mouth；4, three-way valve；5, oil pump；6, heat-conducting oil furnace；7, cold oil storage tank.
Detailed description of the invention
For summary of the invention of the present utility model, feature and effect can be further appreciated that, hereby lift following example and describe in detail as follows:
This utility model includes nutrient media components carries out predissolve and the first reactor 2 of stirring and the second reactor 3 of preparation generation anaerobic culture medium, and nutrient media components is thoroughly mixed and flows to the second reactor 3 afterwards by the first reactor 2.In the present embodiment, the volume of the first reactor 2 is 80L, and the volume of the second reactor 3 is 300L.
Referring to Fig. 2 and Fig. 3, the concrete structure of the first reactor 2 is:
First reactor 2 includes the first tank body 2-2 supported by supporting leg 2-4 and the rabbling mechanism being made up of the first reductor 2-1 and the first stirring paddle 2-5, it is coated with the first heating jacket 2-3 in the outside of the first tank body 2-2, offer the first drain hole 2-7 in the bottom of the first tank body 2-2, the roof of the first tank body 2-2 is provided with the first charging aperture 2-12 and the first purified water entrance 2-14.Being provided with the first hot oil outlet 2-6 in the bottom of the first heating jacket 2-3, the top side wall at the first heating jacket 2-3 is provided with the first deep fat entrance 2-11, connects and install oil spilling groove 2-10 at the first deep fat entrance 2-11.Conduction oil flows in the first heating jacket 2-3, and the inner chamber of the first tank body 2-2 is carried out continuous heating.
The roof of the first tank body 2-2 is additionally provided with the first temperature-measuring port 2-8, the sidewall of the first heating jacket 2-3 is additionally provided with the second temperature-measuring port 2-9, in reactor, install temperature element additional by above-mentioned temperature-measuring port, monitor temperature and the temperature of the first heating jacket 2-3 of the first tank body 2-2 inner chamber in course of reaction.
The roof of the first tank body 2-2 is additionally provided with the first drinking water entrance 2-13.
Referring to Fig. 4 and Fig. 5, the concrete structure of the second reactor 3 is:
Second reactor 3 includes the second tank body 3-2 supported by supporting leg 3-4 and the rabbling mechanism being made up of the second reductor 3-1 and the second stirring paddle 3-5, is coated with cooling coil in the outside of the second tank body 3-2, is coated with the second heating jacket 3-3 in the outside of cooling coil.Being provided with the second hot oil outlet 3-6 in the bottom of the second heating jacket 3-3, the top side wall at the second heating jacket 3-3 is provided with the second deep fat entrance 3-15, connects and install oil spilling groove 3-13 at the second deep fat entrance 3-15.The upper end of cooling coil is sealed by the top of the second heating jacket 3-3 sidewall to pass and constitutes the second cold oil outlet 3-16, and lower end is sealed to pass by the sidewall bottom of the second heating jacket 3-3 and constitutes the second cold oil entrance 3-8.It is provided with the second drain hole 3-7 in the bottom of the second tank body 3-2, the roof of the second tank body arranges the second charging aperture 3-21, the roof of the second tank body 3-2 is additionally provided with the second purified water entrance 3-20, nitrogen inlet 3-17 and dropping mouth 3-18.
The roof of the second tank body 3-2 is additionally provided with the 3rd temperature-measuring port 3-11, the sidewall of the second heating jacket 3-3 is additionally provided with the 4th temperature-measuring port 3-14, in reactor, install temperature element additional by above-mentioned temperature-measuring port, monitor temperature and the temperature of the second heating jacket 3-3 of the second tank body 3-2 inner chamber in course of reaction.
In order to the liquid level in the second tank body 3-2 is monitored, in the present embodiment, the sidewall of the second tank body 3-2 is additionally provided with upper and lower two interface of the level gauge 3-10, by arranging liquidometer, liquid level is measured.
The roof of the second tank body 3-2 is additionally provided with the second drinking water entrance 3-19 and compressed air inlet 3-22；The roof of the second tank body 3-2 is also equipped with mirror mouth 3-23；The roof of the second tank body 3-2 is also equipped with hand hole 3-9；The sidewall of the second tank body 3-2 is provided with air outlet slit 3-12.
System building mode:
First drain hole 2-7 of the first reactor 2 is connected to the second charging aperture 3-21 of the second reactor 3 by pipeline.
Also include heat-conducting oil furnace 6, cold oil storage tank 7 and oil pump 5, the oil-out of both heat-conducting oil furnace 6 and cold oil storage tank 7 and the oil-in of oil pump 5 are connected by three-way valve 4 and pipeline, the oil return opening of the first hot oil outlet 2-6 of the first reactor 2, the second hot oil outlet 3-6 of the second reactor 3 and heat-conducting oil furnace 6 is connected by three-way valve 4 and pipeline, and the second cold oil outlet 3-16 of the second reactor 3 is connected with the oil return opening of cold oil storage tank 7 by pipeline.
The oil-out of oil pump 5 is provided with three-way valve 4, one outlet of this three-way valve 4 is connected to the second cold oil entrance 3-8 of the second reactor 3, another outlet by pipeline and is provided with the three-way valve 4 of two grades by pipeline, and two outlets of this three-way valve 4 are respectively connecting to the first deep fat entrance 2-11 of the first reactor 2 and the second deep fat entrance 3-15 of the second reactor 3.
Above-mentioned oil pump 5 constitutes the two circuit public pumping element in deep fat loop and cold oil loop, by regulating each three-way valve 4, difference pipeline is accessed operating mode, and concrete operating mode is as follows:
By three-way valve 4, heat-conducting oil furnace 6 is connected with oil pump 5 (now cold oil storage tank 7 does not accesses), by three-way valve 4 by deep fat circuit communication, conduction oil in heat-conducting oil furnace 6 is imported in the heating jacket of two reactors by oil pump 5, and the deep fat refluxed via the hot oil outlet of two reactors reenters heat-conducting oil furnace 6 and participates in circulation；When preparation process terminates, by three-way valve 4, cold oil storage tank 7 is connected with oil pump 5 (now heat-conducting oil furnace 6 does not accesses), by three-way valve semi-finals cold oil circuit communication, cooling oil in cold oil storage tank 7 is imported in the cooling coil of the second reactor 3 by oil pump 5, and the cooling oil of backflow reenters cold oil storage tank 7 and participates in circulation.
In the present embodiment, also include the measuring tank 1 of purified water, second purified water entrance 3-20 of the outlet of measuring tank 1, the first purified water entrance 2-14 of the first reactor 2 and the second reactor 3 uses three-way valve 4 and pipeline to connect, and measures feeding purified water by three-way valve 4 and control system in the first reactor 2 and the second reactor 3.
Work process:
(1) purified water of cumulative volume 20% to be joined first is added during preparation culture medium from the first purified water entrance 2-14 of the first reactor 2, then each for load weighted culture medium component (in addition to thermo-labile component and reducing agent) is added in reactor from the first charging aperture 2-12, open stirring and heating, make culture medium fully boil dissolving.
The purified water of culture medium cumulative volume to be joined 75% it is previously added in (2) second reactors 3, import conduction oil from the second deep fat entrance 3-15 to begin to warm up, purified water ebuillition of heated removes oxygen, after boiling, conduction oil is derived, connect cooling oil cooling, simultaneously by nitrogen inlet 3-17 the supply of nitrogen in reactor, by ventilation, the air in reactor is replaced as nitrogen, is separated out the oxygen impact on culture medium.
After in (3) first reactors 2, culture medium boils dissolving, import in the second reactor 3 from the first drain hole 2-7, culture medium mixes with the purified water boiling deoxygenation, after temperature drops to less than 90 DEG C, from the second charging aperture 3-21, thermo-labile component and reducing agent are added to culture medium, being passed through nitrogen and stir, then constant volume, liquid level can be observed by liquidometer.
(4) regulate pH value after constant volume, i.e. add acid from dropping mouth 3-18 or aqueous slkali regulates pH value, whole during logical nitrogen gas stirring, it is ensured that anaerobic culture medium anaerobic.
Claims (10)
1. an anaerobic culture medium preparation system, it is characterized in that: including carrying out nutrient media components the first reactor (2) and second reactor (3) of preparation generation anaerobic culture medium of predissolve and stirring, first drain hole (2-7) of the first reactor (2) is connected to second charging aperture (3-21) of the second reactor (3) by pipeline；First tank body (2-2) of the first reactor (2) is provided with the first heating jacket (2-3) with conduction oil as medium, and second tank body (3-2) of the second reactor (3) is provided with the second heating jacket (3-3) with conduction oil as medium and the cooling coil with cooling oil as medium；nullAlso include heat-conducting oil furnace (6)、Cold oil storage tank (7) and oil pump (5)，The oil-in of heat-conducting oil furnace (6) and cold oil storage tank (7) both oil-outs and oil pump (5) is connected by three-way valve (4) and pipeline，First hot oil outlet (2-6) of the first reactor (2)、Second hot oil outlet (3-6) of the second reactor (3) and the oil return opening of heat-conducting oil furnace (6) are connected by three-way valve (4) and pipeline，Second cold oil outlet (3-16) of the second reactor (3) is connected with the oil return opening of cold oil storage tank (7) by pipeline，The oil-out of oil pump (5) is provided with three-way valve (4)，One outlet of this three-way valve (4) is connected to the second cold oil entrance (3-8) of the second reactor (3) by pipeline、Another outlet is provided with three-way valve (4) by pipeline，Two outlets of this three-way valve (4) are respectively connecting to the first deep fat entrance (2-11) of the first reactor (2) and the second deep fat entrance (3-15) of the second reactor (3).
2. anaerobic culture medium preparation system as claimed in claim 1, it is characterized in that: the first reactor (2) includes the first tank body (2-2) and the rabbling mechanism being made up of the first reductor (2-1) and the first stirring paddle (2-5), first heating jacket (2-3) is coated on the outside of the first tank body (2-2), and the first drain hole (2-7) is positioned at the bottom of the first tank body (2-2)；The roof of the first tank body (2-2) is provided with the first charging aperture (2-12) and the first purified water entrance (2-14).
3. anaerobic culture medium preparation system as claimed in claim 2, it is characterized in that: on the roof of the first tank body (2-2), be additionally provided with the first temperature-measuring port (2-8), the sidewall of the first heating jacket (2-3) is additionally provided with the second temperature-measuring port (2-9).
4. anaerobic culture medium preparation system as claimed in claim 2, it is characterised in that: on the roof of the first tank body (2-2), it is additionally provided with the first drinking water entrance (2-13).
5. anaerobic culture medium preparation system as claimed in claim 1, it is characterized in that: the second reactor (3) includes the second tank body (3-2) and the rabbling mechanism being made up of the second reductor (3-1) and the second stirring paddle (3-5), cooling coil is coated on the outside of the second tank body (3-2), and the second heating jacket (3-3) is coated on the outside of cooling coil；Be provided with the second drain hole (3-7) in the bottom of the second tank body (3-2), the second charging aperture (3-21) is arranged on the roof of the second tank body (3-2)；The roof of the second tank body (3-2) is additionally provided with the second purified water entrance (3-20), nitrogen inlet (3-17) and dropping mouth (3-18).
6. anaerobic culture medium preparation system as claimed in claim 5, it is characterized in that: on the roof of the second tank body (3-2), be additionally provided with the 3rd temperature-measuring port (3-11), the sidewall of the second heating jacket (3-3) is additionally provided with the 4th temperature-measuring port (3-14).
7. anaerobic culture medium preparation system as claimed in claim 5, it is characterised in that: on the roof of the second tank body (3-2), it is additionally provided with the second drinking water entrance (3-19) and compressed air inlet (3-22).
8. anaerobic culture medium preparation system as claimed in claim 5, it is characterised in that: on the roof of the second tank body (3-2), it is also equipped with mirror mouth (3-23).
9. anaerobic culture medium preparation system as claimed in claim 5, it is characterised in that: on the roof of the second tank body (3-2), it is also equipped with hand hole (3-9).
10. the anaerobic culture medium preparation system as described in any one of claim 1 to 9, it is characterized in that: also include the measuring tank (1) of purified water, the second purified water entrance (3-20) of the outlet of measuring tank (1), the first purified water entrance (2-14) of the first reactor (2) and the second reactor (3) uses three-way valve (4) and pipeline to connect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620127468.1U CN205443257U (en) | 2016-02-18 | 2016-02-18 | Anaerobic culture medium prepares system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620127468.1U CN205443257U (en) | 2016-02-18 | 2016-02-18 | Anaerobic culture medium prepares system |
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| Publication Number | Publication Date |
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| CN205443257U true CN205443257U (en) | 2016-08-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201620127468.1U Expired - Fee Related CN205443257U (en) | 2016-02-18 | 2016-02-18 | Anaerobic culture medium prepares system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109464972A (en) * | 2018-10-26 | 2019-03-15 | 惠州市强茂化工科技有限公司 | Acrylic acid reaction unit |
| WO2022205992A1 (en) * | 2021-04-01 | 2022-10-06 | 湖北文理学院 | Culture medium manufacturing system |
-
2016
- 2016-02-18 CN CN201620127468.1U patent/CN205443257U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109464972A (en) * | 2018-10-26 | 2019-03-15 | 惠州市强茂化工科技有限公司 | Acrylic acid reaction unit |
| CN109464972B (en) * | 2018-10-26 | 2021-03-02 | 惠州市强茂化工科技有限公司 | Acrylic acid reaction device |
| WO2022205992A1 (en) * | 2021-04-01 | 2022-10-06 | 湖北文理学院 | Culture medium manufacturing system |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160810 |
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| CF01 | Termination of patent right due to non-payment of annual fee |