LU500097B1

LU500097B1 - Tissue Homogenizer for Primary Muscle Cell Culture of Livestock

Info

Publication number: LU500097B1
Application number: LU500097A
Authority: LU
Inventors: Rongsheng Zhu; Jianying Wang; Xukui Cui; Zhaohua Liu; Xiuwen Tan; Ke Wang
Original assignee: Inst Animal Science & Veterinary Medicine Shandong Aas
Priority date: 2021-04-29
Filing date: 2021-04-29
Publication date: 2021-10-29

Abstract

A tissue homogenizer for primary muscle cell culture of livestock is disclosed in the invention, which relates to the field of cell culture. The technical scheme specifically comprises a bottom platform which is composed of a plurality of vertically arranged lower supporting rods. Further, the bottom platform is connected with an upper bracket through the lower supporting rods. The upper bracket comprises a plurality of guide rods corresponding to the lower supporting rods, and the bottom ends of the guide rods are inserted with the top ends of the lower supporting rods. The top end of the guide rod is provided with a horizontal bearing plate, and the lower side of the bearing plate is provided with an extrusion block. The guide rod is slidably connected with the homogenizing assembly. This device uses a container with adjustable bottom as the carrier for the muscle particles.

Description

DESCRIPTION Tissue Homogenizer for Primary Muscle Cell Culture of Livestock

TECHNICAL FIELD The invention relates to the field of cell culture equipment, in particular to a tissue homogenizer for primary muscle cell culture of livestock.

BACKGROUND Muscle cell culture is a significantly important link in the study of primary muscle of livestock. After the muscle tissues of livestock are collected and brought back to the laboratory immediately, the tissues are washed several times in the clean bench with normal saline or PBS solution, and then cut into tissue particles with scissors. Besides, the size of the tissue particles cannot be larger than 0.5mm?, otherwise they are easy to float after being inoculated into a culture flask or petri dish with culture medium and cannot grow attached to the wall. During the operation, it can be found that it was time- consuming and laborious to cut the muscle tissue so small with scissors, and the size of the tissue was uneven. Due to the uneven size of tissue particles, it is difficult to control the time of adding culture solution after inoculation into culture flask or petri dish. If it is observed that small tissue particles have adhered to the wall, the larger tissue granules are easy to float when the culture medium is added; if the culture medium is added when the larger tissue granules are observed to adhere to the wall, the smaller tissue particles may lose their activity due to drying, resulting in the inability to proliferate and grow.

SUMMARY In view of the above technical problems, the present invention provides a tissue homogenizer for primary muscle cell culture of livestock.

According to the technical scheme, the tissue homogenizer for primary muscle cell culture of livestock comprises a bottom platform which comprises a plurality of vertically arranged lower supporting rods, wherein the bottom platform is connected with an upper bracket through the lower supporting rods.

The upper bracket comprises a plurality of guide rods corresponding to the lower supporting rods, and the bottom ends of the guide rods are inserted with the top ends of the lower supporting rods.

The top end of the guide rod is provided with a horizontal bearing plate, and the lower side of the bearing plate is provided with an extrusion block.

The guide rod is slidably connected with a homogenizing assembly, which comprises an outer cylinder and an inner cylinder which are sleeved.

The outer cylinder and the inner cylinder are both cylindrical cylinders, and they are both hollow inside.

The peripheral walls of them are made of rigid materials.

The top end of the outer cylinder is open, while the top end of the inner cylinder is provided with a top membrane made of inextensible flexible materials.

The natural state of the top membrane is hemispherical.

The bottom end of the inner cylinder is equipped with a liquid inlet, and the inner cavity of the inner cylinder is communicated with the inside of the liquid box arranged in the bottom platform through a liquid inlet pipeline and a liquid pump.

A driving assembly for driving the homogenizing assembly to vertically slide along the guide rod is also arranged in the bottom platform.

Preferably, the liquid inlet pipeline is a flexible bellows and the liquid in the inner cylinder and the liquid box is hydraulic oil.

When the device is used, the pre-taken livestock primary muscle granules are put into the homogenizing assembly, and the guide rods of the upper bracket are butt-connected with the lower supporting rods.

The initial state of the inner cylinder is filled with liquid, so that the top membrane protrudes into a hemispherical shape.

Under the action of the driving assembly, the homogenizing assembly moves upwards to contact the extrusion block, thereby forming an extrusion for the muscle granules to make them smaller.

After completing a squeeze, a part of liquid in the inner cylinder is withdrawn, and the volume V of the withdrawn liquid conforms to the formula of V = sr, wherein r is equal to the radius of the inner ring of the top face of the inner cylinder.

Then the homogenizing assembly is lifted again by the driving assembly, so that the extrusion block is in contact with the top membrane.

Under the thrust given by the driving assembly, and combined with the top membrane itself, the muscle granules are squeezed twice because of the folds that change from a spherical surface to a plane.

After the second extrusion is completed, the liquid in the inner cylinder is pumped out of the volume V again.

At this time, the top membrane becomes a concave spherical surface, and the steps of lifting the homogenizing assembly are repeated to achieve the third extrusion of the muscle granules.

Repeat the above process several times to complete the homogenization process of the muscle granules, thereby muscle granules are turned from granular to paste.

The paste form can better fit the inner wall of the culture container, so that it can be inoculated better, and after inoculation, the paste form is not easily affected by the injection of the culture solution, which helps to improve the quality of cell culture.

Preferably, the extrusion block comprises a spherical block (231) corresponding to the inner diameter of the top end of the inner cylinder (320). The spherical block can better fit the concave state of the top membrane to obtain a better homogenization effect.

Preferably, a closed cylinder 1s arranged on the peripheral side of the spherical block of the extrusion block, wherein the inner diameter of the closed cylinder corresponds to the outer diameter of the outer cylinder, and a sealing ring is arranged at the lower end of the closed cylinder.

The inner top of the closed cylinder is located above the horizontal section of the spherical block, and the horizontal section passes through the center of the spherical block.

The horizontal plane of the bottom end of the closed cylinder is below the bottom end face of the spherical block.

Through the closed cylinder, when the homogenizing assembly moves upwards, the closed cylinder is sleeved on the outside of the outer cylinder, and the air in the closed cylinder is difficult to discharge through the sealing ring, especially when the top membrane is in a concave state.

Because the air is easily compressed, and the internal air volume is small, it will not affect the normal extrusion homogenization.

Moreover, the compressed air can prevent the block from being completely attached to the top membrane, thereby avoiding the muscle granules from being excessively squeezed and causing cell damage.

Preferably, an elastic pad is arranged at the top of the extrusion block.

When this device is used, in order to ensure the extrusion force of the extrusion block, the user presses down on the top of the upper bracket from above by hand.

The pressing position is like the outer shell of the top motor.

When downward pressure is applied, a thrust in the reverse direction is generated, because the closed cylinder makes it difficult to discharge the air inside.

The elastic pad can have a better cushioning effect on this force, and the contraction of the elastic pad can also prevent the muscle tissue cells from being damaged by excessive extrusion.

Preferably, a top motor is fixedly arranged on the upper side of the bearing plate, wherein the motor shaft of the top motor penetrates through the bearing plate and is linked with the spherical block.

The motor shaft of the top motor is coaxial with the spherical block.

Preferably, the closed cylinder is connected with the spherical block through a bearing.

The connection form here can be selected as follows.

The top of the closed cylinder is fixedly connected with the lower side of the bearing plate, the spherical block is connected with the inner wall of the closed cylinder through the bearing, and the top of the spherical block is connected with the motor shaft of the top motor through the elastic pad.

The spherical block is driven by the top motor to make the spherical block rotate or twist back and forth.

When the spherical block contacts with the muscle granules, it can achieve better homogenization effect.

Preferably, horizontal plates are symmetrically arranged on both sides of the lower part of the outer cylinder, and through holes are formed in the horizontal plates.

Further, the horizontal plates are slidably connected with the guide rod through the through holes.

The driving assembly comprises a cam positioned below the horizontal plate, and the axle of the cam is linked with the motor shaft of the lower motor.

The wheel body of the cam is provided with an axle hole as well as four outer convex surfaces positioned at the peripheral side of the axle hole, wherein each outer convex surface is an arc surface, and a plane is formed between two adjacent outer convex surfaces.

The connecting line from the center of the axle hole to the farthest point of each convex surface is in a cross shape, and the length of the connecting line increases by r in order of clockwise or counterclockwise. r is equal to the radius of the inner ring of the top face of the inner cylinder.

And the length of the line connecting the center of the axle hole to the distal point of the nearest convex surface 1s equal to r.

With the help of the above design, the initial state of the device is that the outer convex surface closest to the cam and axle hole is on the upper side, and the bottom surface of the horizontal plate is close to the surface, wherein the distance between the bottom end of the spherical block and the top of the top membrane in the protruding state is 7. When the cam rotates 90°, the horizontal plate is lifted and moved for a distance of r.

At this time, the bottom end of the spherical block contacts the top of the top membrane for the first extrusion.

When the second extrusion, the top membrane retracts a distance of r, the cam rotates 90° again, and the lifting distance of the horizontal plate relative to the initial position is 2r.

By analogy, when the third extrusion, the lifting distance of the horizontal plate relative to the initial position is 3r.

After the cam continues to rotate 90°, it resets.

Repeat the above operation to finish homogenization.

The advantage of this structure is that it can be combined with a stepper motor as a cam, and the quantitative lifting of the horizontal plate can be realized by turning the cam 90° each time.

Compared with the hydraulic and pneumatic push rod, the cam quantitative drive can make the device run more stable, and the cost is far lower than the pneumatic and hydraulic drive.

In addition, the cam is used as the lifting mechanism, and other periodic mechanical structures can be combined as the driving structure, so that an ordinary unidirectional motor can also be used to complete the driving.

Preferably, the bottom end of the inner cylinder is fixedly connected with a screw rod, which is hollow and communicated with the inner cylinder.

Besides, the screw rod is provided with a liquid inlet hole, and the bottom end of the screw rod in connected with the liquid inlet pipeline.

The bottom end of the outer cylinder is rotatably connected with an adjusting ring, and the inner side of the adjusting ring is provided with an internal thread which is in threaded connection with the screw rod.

The lower part of the outer cylinder is provided with a vertical limiting groove, and the out lower part of the inner cylinder is fixedly provided with a limiting block corresponding to the limiting groove.

Further, the inner cylinder is in sliding connection with the outer cylinder through the limiting block and the limiting groove.

Through the structure, when the homogenization is completed, the adjusting ring can be rotated to drive the screw rod to move, so as to lift the inner cylinder up, which is convenient for the staff to take out the homogenized meat paste.

A method for culturing primary muscle cells of livestock comprises the following steps.

S1. Obtaining muscle blocks from livestock.

S2. Cutting the block obtained in S1 into muscle granules.

S3. Rolling the muscle granules in S2 to make the muscle granules become pasty.

S4. Inoculating the paste obtained in S3 into a culture container and waiting until the paste completely adheres to the wall.

S5. Adding culture solution.

A method for homogenizing primary muscle cells of livestock by adopting the tissue homogenizer comprises the following steps.

Al. Putting the pre-taken primary muscle granules of livestock into the homogenizing assembly and pumping the inner cylinder with liquid in the initial state, so that the top membrane protrudes into a hemispherical shape. A2. Making the homogenizing assembly move upwards and contact with the extrusion block to form a primary extrusion on muscle granules. A3. Withdrawing a part of liquid in the inner cylinder, and the volume V of the withdrawn liquid meets the formula of V = iur} Wherein r is equal to the radius of the inner ring of the top face of the inner cylinder. A4. Lifting the homogenizing assembly again to make the extrusion block contact with the top membrane and perform the second extrusion of muscle granules. AS. Steps A3 and A4 are repeated for the third extrusion. A6. Circularly repeating steps A2 to AS for several times until the homogenate is prepared. According to the technical scheme provided by the embodiment of the invention, the device takes the container with an adjustable bottom as the carrier for the muscle granules. By adjusting the state of the bottom of the container and combined with the spherical extrusion block, the muscle granules can be fully squeezed. Besides, it can ensure that muscle cells are not destroyed during the extrusion process to the greatest extent.

BRIEF DESCRIPTION OF THE FIGURES Figure 1 is a schematic diagram of the overall structure of an embodiment of the present invention.

Figure 2 is a schematic diagram of a hidden shell according to an embodiment of the present invention. Figure 3 is a schematic structural diagram of an extrusion block according to an embodiment of the present invention. Figure 4 is a schematic diagram of a cam structure according to an embodiment of the present invention. Figure 5 1s a front view of a cam according to an embodiment of the present invention. Figure 6 is a schematic diagram showing the protruding state of the top membrane according to an embodiment of the present invention. Figure 7 is a schematic diagram of a top membrane in a contraction state according to an embodiment of the present invention. Wherein, in figures, 1- bottom platform, 101- lower supporting rod, 102- liquid box, 2- upper bracket, 210- guide rod, 220- bearing plate, 230- extrusion block, 240- top motor, 231- spherical block, 232- closed cylinder, 233- elastic pad, 3- homogenizing assembly, 310- outer cylinder, 311- horizontal plate, 312- adjusting ring, 320- inner cylinder, 321- top membrane, 322- liquid inlet pipeline, 323- screw rod, 4- driving assembly, 410- cam, 411- axle hole, 420- lower motor.

DESCRIPTION OF THE INVENTION In order to make the object, technical scheme and advantages of the present invention clearer, the present invention will be further described in detail with reference to the figures and embodiments. Of course, the specific embodiments described here are only used to explain the present invention and are not used to limit the present invention.

It should be noted that the embodiments in the invention and the features in the embodiments can be combined with each other without conflict.

In the description of the invention, it should be understood that the terms “center”, “lengthways”, “crosswise”, “upper”, “lower”, “front”, “rear”, “left”, “right”, “vertical”, “horizontal”, “top”, “bottom”, “inner” and “outer”. In the description of the invention, 1t is to be understood that the orientation or position relationship indicated by the terms “center”, “lengthways”, “crosswise”, “upper”, “lower”, “front”, “rear”, “left”, “right”, “vertical”, “horizontal”, “top”, “bottom”, “inner” and “outer” is based on the orientation or position relationship shown in the figures.

It is intended to describe the invention creation and simplify the description, rather than indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, so it cannot be understood as a limitation of the invention creation.

In addition, the terms “first”, “second” and the like are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features.

Thus, the features defined by “first”, “second” and the like may explicitly or implicitly include one or more of the features.

In the description of the invention, unless otherwise specified, “multiple” means two or more.

In the description of the invention, it should be noted that unless otherwise specified and limited, the terms “installation”, “connection”, “communication” should be understood in a broad sense, for example, it can be fixed connection, detachable connection or integrated connection.

It also can be connected mechanically or electrically.

It can be directly connected, indirectly connected through an intermediate medium, or communicated inside two elements.

For ordinary technicians in the field, the specific meanings of the above terms in the invention can be understood through specific situations.

Embodiment 1 Referring to figures 1 to 7, the invention provides a tissue homogenizer for primary muscle cell culture of livestock, which comprises a bottom platform (1). Specifically, the bottom platform (1) comprises a plurality of vertically arranged lower supporting rods (101), wherein the bottom platform (1) 1s connected with an upper bracket (2) through the lower supporting rods (101). The upper bracket (2) comprises a plurality of guide rods (210) corresponding to the lower supporting rods (101), and the bottom ends of the guide rods (210) are inserted with the top ends of the lower supporting rods (101). The top end of the guide rod (210) is provided with a horizontal bearing plate (220), and the lower side of the bearing plate (220) is provided with an extrusion block (230). The guide rod (210) is slidably connected with a homogenizing assembly (3), which comprises an outer cylinder (310) and an inner cylinder (320) which are sleeved.

The outer cylinder (310) and the inner cylinder (320) are both cylindrical cylinders, and they are both hollow inside.

The peripheral walls of them are made of rigid materials.

The top end of the outer cylinder (310) is open, while the top end of the inner cylinder (320) is provided with a top membrane (321) made of inextensible flexible materials.

The natural state of the top membrane (321) is hemispherical.

The bottom end of the inner cylinder (320) 1s equipped with a liquid inlet, and the inner cavity of the inner cylinder (320) is communicated with the inside of the liquid box (102) arranged in the bottom platform (1) through a liquid inlet pipeline (322) and a liquid pump.

A driving assembly (4) for driving the homogenizing assembly (3) to vertically slide along the guide rod (210) 1s also arranged in the bottom platform (1) (1). Preferably, the liquid inlet pipeline (322) 1s a flexible bellows and the liquid in the inner cylinder (320) and the liquid box (102) is hydraulic oil.

When the device is used, the pre-taken livestock primary muscle granules are put into the homogenizing assembly (3), and the guide rods (210) of the upper bracket (2) are butt- connected with the lower supporting rods (101). The initial state of the inner cylinder (320) 1s filled with liquid, so that the top membrane (321) protrudes into a hemispherical shape.

Under the action of the driving assembly (4), the homogenizing assembly (3) moves upwards to contact the extrusion block (230), thereby forming an extrusion for the muscle granules to make them smaller.

In the first extrusion process, because the top membrane (321) 1s protruding, the maximum pressure is exerted on the muscle granules by combining with the extrusion block (230). After completing the first extrusion, a part of liquid in the inner cylinder (320) is withdrawn, and the volume V of the withdrawn liquid conforms to the formula of V = Zr, wherein r is equal to the radius of the inner ring of the top face of the inner cylinder (320). Then the homogenizing assembly (3) is lifted again by the driving assembly (4), so that the extrusion block (230) is in contact with the top membrane (321). Under the thrust given by the driving assembly (4) and combined with the top membrane (321) itself, the muscle granules are squeezed twice because of the folds that change from a spherical surface to a plane.

After the second extrusion 1s completed, the liquid in the inner cylinder (320) 1s pumped out of the volume V again.

At this time, the top membrane (321) becomes a concave spherical surface, and the steps of lifting the homogenizing assembly (3) are repeated to achieve the third extrusion of the muscle granules.

Embodiment 2 On the basis of Embodiment 1, the extrusion block (230) comprises a spherical block (231) corresponding to the inner diameter of the top end of the inner cylinder (320) (320). The spherical block (231) can better fit the concave state of the top membrane (321) to obtain a better homogenization effect.

A closed cylinder (232) is arranged on the peripheral side of the spherical block (231) of the extrusion block (230), wherein the inner diameter of the closed cylinder (232) corresponds to the outer diameter of the outer cylinder (310), and a sealing ring 1s arranged at the lower end of the closed cylinder (232). The inner top of the closed cylinder (232) 1s located above the horizontal section of the spherical block (231), and the horizontal section passes through the center of the spherical block (231). The horizontal plane of the bottom end of the closed cylinder (232) is below the bottom end face of the spherical block (231).

Through the closed cylinder (232), when the homogenizing assembly (3) moves upwards, the closed cylinder (232) 1s sleeved on the outside of the outer cylinder (310), and the air in the closed cylinder (232) 1s difficult to discharge through the sealing ring, especially when the top membrane (321) 1s in a concave state.

Moreover, the compressed air can prevent the spherical block (231) from being completely attached to the top membrane (321), thereby avoiding the muscle granules from being excessively squeezed and causing cell damage.

Embodiment 3 On the basis of the above embodiments, an elastic pad (233) is arranged at the top of the extrusion block (230). When this device is used, in order to ensure the extrusion force of the extrusion block (230), the user presses down on the top of the upper bracket (2) from above by hand.

The pressing position 1s like the outer shell of the top motor (240). When downward pressure is applied, a thrust in the reverse direction is generated, because the closed cylinder (232) makes it difficult to discharge the air inside.

The elastic pad (233) can have a better cushioning effect on this force, and the contraction of the elastic pad (233) can also prevent the muscle tissue cells from being damaged by excessive extrusion.

Embodiment 4 Based on the above embodiments, a top motor (240) is fixedly arranged on the upper side of the bearing plate (220), wherein the motor shaft of the top motor (240) penetrates through the bearing plate (220) and is linked with the spherical block (231). The motor shaft of the top motor (240) is coaxial with the spherical block (231).

The closed cylinder (232) is connected with the spherical block (231) through a bearing.

The connection form here can be selected as follows.

The top of the closed cylinder (232) is fixedly connected with the lower side of the bearing plate (220), the spherical block (231) is connected with the inner wall of the closed cylinder (232) through the bearing, and the top of the spherical block (231) is connected with the motor shaft of the top motor (240) through the elastic pad (233). The spherical block (231) is driven by the top motor (240) to make the spherical block (231) rotate or twist back and forth.

When the spherical block (231) contacts with the muscle granules, it can achieve better homogenization effect.

Embodiment 5 Based on the above embodiments, horizontal plates (311) are symmetrically arranged on both sides of the lower part of the outer cylinder (310), and through holes are formed in the horizontal plates (311). Further, the horizontal plates (311) are slidably connected with the guide rod (210) through the through holes.

The driving assembly (4) comprises a cam positioned below the horizontal plate (311), and the axle of the cam (410) is linked with the motor shaft of the lower motor (420). The wheel body of the cam (410) 1s provided with an axle hole (411) as well as four outer convex surfaces positioned at the peripheral side of the axle hole (411), wherein each outer convex surface is an arc surface, and a plane is formed between two adjacent outer convex surfaces.

The connecting line from the center of the axle hole (411) to the farthest point of each convex surface is in a cross shape, and the length of the connecting line increases by r in order of clockwise or counterclockwise. r is equal to the radius of the inner ring of the top face of the inner cylinder (320). And the length of the line connecting the center of the axle hole (411) to the distal point of the nearest convex surface is equal to 7. With the help of the above design, the initial state of the device is that the outer convex surface closest to the cam (410) and axle hole (411) is on the upper side, and the bottom surface of the horizontal plate (311) is close to the surface, wherein the distance between the bottom end of the spherical block (231) and the top of the top membrane (321) in the raised state is r.

When the cam (410) rotates 90°, the horizontal plate (311) is lifted and moved for a distance of r.

At this time, the bottom end of the spherical block (231) contacts the top of the top membrane (321) for the first extrusion.

When the second extrusion, the top membrane (321) retracts a distance of r, the cam (410) rotates 90° again, and the lifting distance of the horizontal plate (311) relative to the initial position is 2r.

By analogy, when the third extrusion, the lifting distance of the horizontal plate (311) relative to the initial position is 37. After the cam (410) continues to rotate 90°, it resets.

Repeat the above operation to finish homogenization.

The advantage of this structure is that it can be combined with a stepper motor as a cam (410), and the quantitative lifting of the horizontal plate (311) can be realized by turning the cam (410) 90° each time.

Compared with the hydraulic and pneumatic push rod, the cam (410) quantitative drive can make the device run more stable, and the cost 1s far lower than the pneumatic and hydraulic drive.

In addition, the cam (410) is used as the lifting mechanism, and other periodic mechanical structures can be combined as the driving structure, so that an ordinary unidirectional motor can also be used to complete the driving.

Embodiment 6

On the basis of the above embodiments, the bottom end of the inner cylinder (320) 1s fixedly connected with a screw rod (323), which is hollow and communicated with the inner cylinder (320). Besides, the screw rod (323) 1s provided with a liquid inlet hole, and the bottom end of the screw rod (323) in connected with the liquid inlet pipeline (322). The bottom end of the outer cylinder (310) 1s rotatably connected with an adjusting ring (312), and the inner side of the adjusting ring (312) is provided with an internal thread which is in threaded connection with the screw rod (323). The lower part of the outer cylinder (310) 1s provided with a vertical limiting groove, and the out lower part of the inner cylinder (320) 1s fixedly provided with a limiting block corresponding to the limiting groove.

Further, the inner cylinder (320) is in sliding connection with the outer cylinder (310) through the limiting block and the limiting groove.

Through the structure, when the homogenization is completed, the adjusting ring (312) can be rotated to drive the screw rod (323) to move, so as to lift the inner cylinder (320) up, which is convenient for the staff to take out the homogenized meat paste.

Embodiment 7 A culture method of primary muscle cells of livestock comprises the following steps.

S1. Obtaining muscle blocks from livestock.

S2. Cutting the block obtained in S1 into muscle granules.

S3. Rolling the muscle granules in S2 to make the muscle granules become pasty.

S5. Adding culture solution.

Embodiment 8 A method for homogenizing primary muscle cells of livestock by adopting the tissue homogenizer of Claim 1 comprises the following steps.

Al.

Putting the pre-taken primary muscle granules of livestock into the homogenizing assembly (3) and pumping the inner cylinder (320) with liquid in the initial state, so that the top membrane (321) protrudes into a hemispherical shape.

A2. Making the homogenizing assembly (3) move upwards and contact with the extrusion block (230) to form a primary extrusion on muscle granules.

A3. Withdrawing a part of liquid in the inner cylinder (320), and the volume V of the withdrawn liquid meets the formula of V = iur} Wherein r is equal to the radius of the inner ring of the top face of the inner cylinder (320). A4. Lifting the homogenizing assembly (3) again to make the extrusion block (230) (230) contact with the top membrane (321) and perform the second extrusion of muscle granules.

AS.

Steps A3 and A4 are repeated for the third extrusion.

A6. Circularly repeating steps A2 to AS for several times until the homogenate is prepared.

The above description is only a better embodiment of the invention and does not limit the invention.

Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the invention shall be included in the protection scope of the invention.

Claims

1. A tissue homogenizer for primary muscle cell culture of livestock, characterized by comprising a bottom platform (1) which comprises a plurality of vertically arranged lower supporting rods (101), wherein the bottom platform (1) is connected with an upper bracket (2) through the lower supporting rods (101); the upper bracket (2) comprises a plurality of guide rods (210) corresponding to the lower supporting rods (101), and the bottom ends of the guide rods (210) are inserted with the top ends of the lower supporting rods (101); the top end of the guide rod (210) is provided with a horizontal bearing plate (220), and the lower side of the bearing plate (220) is provided with an extrusion block (230); the guide rod (210) is slidably connected with a homogenizing assembly (3), which comprises an outer cylinder (310) and an inner cylinder (320) which are sleeved; the outer cylinder (310) and the inner cylinder (320) are both cylindrical cylinders, and the peripheral walls of them are made of rigid materials; the top end of the outer cylinder (310) is open, while the top end of the inner cylinder (320) is provided with a top membrane (321) made of inextensible flexible materials; the natural state of the top membrane (321) is hemispherical; the bottom end of the inner cylinder (320) is equipped with a liquid inlet, and the inner cavity of the inner cylinder (320) is communicated with the inside of the liquid box (102) arranged in the bottom platform (1) through a liquid inlet pipeline (322) and a liquid pump; a driving assembly (4) for driving the homogenizing assembly (3) to vertically slide along the guide rod (210) is also arranged in the bottom platform (1).

2. The tissue homogenizer for primary muscle cell culture of livestock according to claim 1, characterized in that the extrusion block (230) comprises a spherical block (231) corresponding to the inner diameter of the top end of the inner cylinder (320).

3. The tissue homogenizer for primary muscle cell culture of livestock according to claim 2, characterized in that a closed cylinder (232) is arranged on the peripheral side of the spherical block (231) of the extrusion block (230), wherein the inner diameter of the closed cylinder (232) corresponds to the outer diameter of the outer cylinder (310), and a sealing ring is arranged at the lower end of the closed cylinder (232); the inner top of the closed cylinder (232) is located above the horizontal section of the spherical block (231), and the horizontal section passes through the center of the spherical block (231); the horizontal plane of the bottom end of the closed cylinder (232) is below the bottom end face of the spherical block (231).

4. The tissue homogenizer for primary muscle cell culture of livestock according to claim 3, characterized in that an elastic pad (233) is arranged at the top of the extrusion block (230).

5. The tissue homogenizer for primary muscle cell culture of livestock according to claim 1, characterized in that a top motor (240) is fixedly arranged on the upper side of the bearing plate (220), wherein the motor shaft of the top motor (240) penetrates through the bearing plate (220) and is linked with the spherical block (231).

6. The tissue homogenizer for primary muscle cell culture of livestock according to claims 1-5, characterized in that the closed cylinder (232) is connected with the spherical block (231) through a bearing.

7. The tissue homogenizer for primary muscle cell culture of livestock according to claims 1-6, characterized in that horizontal plates (311) are symmetrically arranged on both sides of the lower part of the outer cylinder (310), and through holes are formed in the horizontal plates (311); further, the horizontal plates (311) are slidably connected with the guide rod (210) through the through holes; the driving assembly (4) comprises a cam (401) positioned below the horizontal plate (311), and the axle of the cam (410) 1s linked with the motor shaft of the lower motor (420); the wheel body of the cam (410) is provided with an axle hole (411) as well as four outer convex surfaces positioned at the peripheral side of the axle hole (411), wherein each outer convex surface is an arc surface, and a plane is formed between two adjacent outer convex surfaces; the connecting line from the center of the axle hole (411) to the farthest point of each convex surface is in a cross shape, and the length of the connecting line increases by r in order of clockwise or counterclockwise; r is equal to the radius of the inner ring of the top face of the inner cylinder (320); and the length of the line connecting the center of the axle hole (411) to the distal point of the nearest convex surface is equal to 7.

8. The tissue homogenizer for primary muscle cell culture of livestock according to claim 1, characterized in that the bottom end of the inner cylinder (320) is fixedly connected with a screw rod (323), which is hollow and communicated with the inner cylinder (320); besides, the screw rod (323) is provided with a liquid inlet hole, and the bottom end of the screw rod (323) in connected with the liquid inlet pipeline (322);

the bottom end of the outer cylinder (310) is rotatably connected with an adjusting ring (312), and the inner side of the adjusting ring (312) is provided with an internal thread which 1s in threaded connection with the screw rod (323); the lower part of the outer cylinder (310) 1s provided with a vertical limiting groove, and the out lower part of the inner cylinder (320) 1s fixedly provided with a limiting block corresponding to the limiting groove; further, the inner cylinder (320) is in sliding connection with the outer cylinder (310) through the limiting block and the limiting groove.

9. A method for culturing primary muscle cells of livestock, characterized by comprising the following steps; S1. obtaining muscle blocks from livestock; S2. cutting the block obtained in S1 into muscle granules; S3. rolling the muscle granules in S2 to make the muscle granules become pasty; S4. inoculating the paste obtained in S3 into a culture container and waiting until the paste completely adheres to the wall; S5. adding culture solution.

10. A method for homogenizing primary muscle cells of livestock, characterized by adopting the tissue homogenizer of claim 1, comprising the following steps; Al. putting the pre-taken primary muscle granules of livestock into the homogenizing assembly (3) and pumping the inner cylinder (320) with liquid in the initial state, so that the top membrane (321) protrudes into a hemispherical shape; A2. making the homogenizing assembly (3) move upwards and contact with the extrusion block (230) to form a primary extrusion on muscle granules;

A3. withdrawing a part of liquid in the inner cylinder (320), and the volume V of the withdrawn liquid meets the formula of V = Zr wherein r is equal to the radius of the inner ring of the top face of the inner cylinder (320); A4. lifting the homogenizing assembly (3) again to make the extrusion block (230) contact with the top membrane (321) and perform the second extrusion of muscle granules; AS. steps A3 and A4 are repeated for the third extrusion; A6. circularly repeating steps A2 to AS for several times until the homogenate is prepared.