LT2013069A - Seleno baltymų (selprot) gavimas - Google Patents

Seleno baltymų (selprot) gavimas

Info

Publication number
LT2013069A
LT2013069A LT2013069A LT2013069A LT2013069A LT 2013069 A LT2013069 A LT 2013069A LT 2013069 A LT2013069 A LT 2013069A LT 2013069 A LT2013069 A LT 2013069A LT 2013069 A LT2013069 A LT 2013069A
Authority
LT
Lithuania
Prior art keywords
selenocysteine
selenoproteins
yest
protein
purified
Prior art date
Application number
LT2013069A
Other languages
English (en)
Other versions
LT6159B (lt
Inventor
Saulius Klimašauskas
Rasa Rakauskaitė
Viktoras Masevičius
Original Assignee
Vilniaus Universitetas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vilniaus Universitetas filed Critical Vilniaus Universitetas
Priority to LT2013069A priority Critical patent/LT6159B/lt
Priority to EP14755175.8A priority patent/EP3019194B1/en
Priority to PCT/LT2014/000009 priority patent/WO2015005756A1/en
Publication of LT2013069A publication Critical patent/LT2013069A/lt
Publication of LT6159B publication Critical patent/LT6159B/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C391/00—Compounds containing selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Pritaikius UAG kodono supresiją, vykdomą genetiškai modifikuoto mielių kamieno, pasiūlytas bendras metodas, leidžiantis į bet kurią baltymo poziciją įjungti šviesai jautria grupe apsaugotą selenocisteiną (Sec). Baltymas, kurio sudėtyje yra selenocisteino liekana(-os), apsaugota fotoreaktyvia nitrobenzilo grupe, yra sintetinamas ir išgryninamas iš mielių ląstelių. Apsauginė grupė pašalinama apšvitinus baltymą (esantį ląstelėse arba išgrynintą) UV šviesa, ir tokiu būdu atkuriant Sec. Pasiūlytas metodas galėtų palengvinti selenobaltymų sintezę ir tuo pačiu išplėsti jų biotechnologinį bei biofarmacinį potencialą.
LT2013069A 2013-07-09 2013-07-09 Seleno baltymų (selprot) gavimas LT6159B (lt)

Priority Applications (3)

Application Number Priority Date Filing Date Title
LT2013069A LT6159B (lt) 2013-07-09 2013-07-09 Seleno baltymų (selprot) gavimas
EP14755175.8A EP3019194B1 (en) 2013-07-09 2014-07-08 Production of selenoproteins (selprot)
PCT/LT2014/000009 WO2015005756A1 (en) 2013-07-09 2014-07-08 Production of selenoproteins (selprot)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
LT2013069A LT6159B (lt) 2013-07-09 2013-07-09 Seleno baltymų (selprot) gavimas

Publications (2)

Publication Number Publication Date
LT2013069A true LT2013069A (lt) 2015-01-26
LT6159B LT6159B (lt) 2015-05-25

Family

ID=51392317

Family Applications (1)

Application Number Title Priority Date Filing Date
LT2013069A LT6159B (lt) 2013-07-09 2013-07-09 Seleno baltymų (selprot) gavimas

Country Status (3)

Country Link
EP (1) EP3019194B1 (lt)
LT (1) LT6159B (lt)
WO (1) WO2015005756A1 (lt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
LT6905B (lt) * 2020-07-24 2022-04-25 Vilniaus Universitetas Antikūnai fotoaktyvių baltymų manipuliavimui

Also Published As

Publication number Publication date
LT6159B (lt) 2015-05-25
EP3019194B1 (en) 2018-10-17
EP3019194A1 (en) 2016-05-18
WO2015005756A1 (en) 2015-01-15

Similar Documents

Publication Publication Date Title
DK3523423T3 (da) Fremgangsmåder til målrettet differentiering af pluripotente stamceller til hla homozygote immunceller
MX364690B (es) Proceso de elaboracion escalable para producir vectores lentivirales recombinantes en un sistema de cultivo celular en suspension libre de suero.
SG10201900948PA (en) Modified nucleotide linkers
MX2017010407A (es) Proceso para la produccion de acido acrilico.
PH12015501477A1 (en) Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells
SA515361251B1 (ar) طريقة لزيادة تكوين حمض بيرو-جلوتاميك من بروتين
EP2401775A4 (en) LIGHT SOURCES WITH SEGMENT LED FOR COMPENSATING MANUFACTURE DEVIATIONS IN THE LIGHTING PERFORMANCE OF INDIVIDUAL SEGMENTED LED
MX2015012875A (es) Composiciones de cultivo celular con antioxidantes y metodos para la produccion de polipeptidos.
EP3292198A4 (en) Reversion of primed pluripotent stem cells to naive pluripotent stem cells
BR112016016539A2 (pt) micro-organismos recombinantes enriquecidos com carboidratos
NZ703973A (en) Complex chromosome engineering for production of human antibodies in transgenic animals
MX2020001357A (es) Produccion microbiana de aminas grasas.
EP3443073A4 (en) DIFFERENTIATION OF PLURIPOTENT STEM CELLS IN MEDIUM INTESTINE ENDODERM CELLS
MX2019002223A (es) Procedimiento mejorado para el cultivo de algas.
EP3140392C0 (en) PRODUCTION OF HETEROMULTIMERIC PROTEINS BY MEANS OF MAMMALIAN CELLS
EP2799552A4 (en) METHOD CAPABLE OF INDUCING MASSIVELY AND RAPIDLY MESENCHYMAL STEM CELLS FOR TRANS-DETERMINATION IN HEMATOPOIETIC STEM CELLS OF HIGH PURITY
HK1223979A1 (zh) 通過毒胡蘿蔔細胞懸浮培養生產毒胡蘿蔔素
MY198268A (en) Omega-hydroxylase-related fusion polypeptides with improved properties
KR101896803B9 (ko) 인간 만능줄기세포로부터 중간엽 줄기세포로의 분화 유도 생산율을 증가시키는 방법 및 이에 의해 생성된 중간엽 줄기세포
IN2014DN09097A (lt)
WO2014182684A3 (en) Methods and compositions related to large scale production of proteins
MX2018007298A (es) Variantes con mejores propiedades de polipéptidos de fusión relacionados con omega-hidroxilasa.
LT2013069A (lt) Seleno baltymų (selprot) gavimas
CL2015003069A1 (es) Metodo para separar y purificar lactoferrina recombinante humana a partir de semillas de arroz
EA201790360A1 (ru) Получение полностью процессированного и функционального фактора x в фурин-секретирующей системе экспрессии млекопитающего

Legal Events

Date Code Title Description
BB1A Patent application published

Effective date: 20150126

FG9A Patent granted

Effective date: 20150525

MM9A Lapsed patents

Effective date: 20160709