KR970706495A - 신호 활성표면에 공유결합으로 고정된 생체물질을 갖는 바이오센서(biosensor with biomaterial covalently fixed on a signal-active surface) - Google Patents

신호 활성표면에 공유결합으로 고정된 생체물질을 갖는 바이오센서(biosensor with biomaterial covalently fixed on a signal-active surface)

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Publication number
KR970706495A
KR970706495A KR1019970702298A KR19970702298A KR970706495A KR 970706495 A KR970706495 A KR 970706495A KR 1019970702298 A KR1019970702298 A KR 1019970702298A KR 19970702298 A KR19970702298 A KR 19970702298A KR 970706495 A KR970706495 A KR 970706495A
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South Korea
Prior art keywords
biosensor
group
signal
crosslinking agent
active surface
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KR1019970702298A
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English (en)
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마리온 투스트
미카엘 요세프 쇠닝
요아힘 페터
울리크 벤자민 카우프
피터 코르도스
한스 뤼트
Original Assignee
게이로크 파우. 크리칭, 하랄트 뮐러-몬트
포르슝스젠트룸 율리히 게엠베하
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Publication of KR970706495A publication Critical patent/KR970706495A/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Photoreceptors In Electrophotography (AREA)
  • Holo Graphy (AREA)

Abstract

신호 활성표면에 대해 반응하는 기 A(예: -OH, -SH 또는 -NHX와 반응하는 기) 및 광에 의해 활성화될 수 있는 생체 물질 반응성 기 B를 갖는 헤테로이작용성 가교결합제에 의해 생활성 물질, 특히 효소를 바이오센서의 신호 활성표면에 성공적으로 결합시킬 수 있다. 기 B는 공지되어 있고, 특히 아지도페닐 및 아지도살리실계 기이다. 한편으로 이러한 기 B를 함유하고 다른 한편으로는 숙신이미드 에스테르 기를 함유하는 가교결합제는 시판 중이므로 특히 바람직하다. 신호 활성표면 SiH4/NH3반응에 의해, 특히 신선한 SiO2표면상에서 기체상으로부터 수득될 수 있고 특별히 묽은 플루오르화수소산을 사용하는 처리에 의해 산화물로부터 유리되는 바와 같이 반응성 NHX기를 갖는 Si3H4표면일 수 있다.

Description

신호 활성표면에 공유결합으로 고정된 생체물질을 갖는 바이오센서(BIOSENSOR WITH BIOMATERIAL COVALENTLY FIXED ON A SIGNAL-ACTIVE SURFACE)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 센서의 원리(측정 배치)를 도시하고, 제2도는 농도 범위 10-4내지 10-1Mol/1에 대한 전형적인 측정곡선을 나타내고, 제3도는 본 발명에 따른 센서의 검출곡선을 나타낸다.

Claims (9)

  1. 센서형 생체물질이 가교결합제에 의해 결합되고, 경우에 따라 실란화된 신호활성표면을 갖는 바이오센서, 특히 전계효과를 기본으로 하는 바이오센서에 있어서, 가교결합제가 신호 활성표면을 위해 한편으로 특정 반응성 기(A)를 함유하고 다른 한편으로는 광에 의해 활성화될 수 있는 생체물질 반응성 기(B)를 함유하는 헤테로이작용성 가교결합제에 의해 형성되는 것을 특징으로 하는 바이오센서.
  2. 제1항에 있어서, 가교결합제가 신호 활성표면의 OH-, SH 또는 -NHX기와 가교결합제의 기(A)의 결합반응에 의해 신호 활성표면에 결합되는 것을 특징으로 하는 바이오센서.
  3. 제2항에 있어서, 가교결합제가 가교결합제의 기(A)의 결합 반응에 의해 신호 활성표면 또는 반응성 -NHX기를 갖는 Si3N4- 표면에 결합되는 것을 특징으로 하는 바이오센서.
  4. 제1항 내지 제3항 중의 어느 한 항에 있어서, 가교결합제가 가교결합제의 아지도- 또는 니트로-기(B)의 광활성 결합 반응에 의해 생체물질의 단백질에 결합되는 것을 특징으로 하는 비이오센서.
  5. 제4항에 있어서, 광활성 결합 반응이 방향족 결합된 니트로- 또는 아지도-기(B)를 사용하여 수행되는 것을 특징으로 하는 바이오센서.
  6. 제5항에 있어서, 결합이 아지도페닐 기를 사용하는 가교결합제에 의해 수행되는 것을 특징으로 하는 바이오센서.
  7. 제2항에 있어서, 가교결합제가 알데히드-, 에스테르 또는 이미도에스테르- 기(A)의 결합반응에 의해 표면에 결합되는 것을 특징으로 하는 바이오센서.
  8. 제7항에 있어서, 가교결합제가 N-히드록시-숙신이미드에스테로-기(A)의 결합 반응에 의해 표면에 결합되는 것을 특징으로 하는 바이오센서.
  9. 제1항 내지 제8항 중의 어느 한 항에 있어서, 신호 활성표면의 두께가 10 내지 1000㎚이고 SiH4/NH3-혼합물로 구성된 CVD에 의해 SiO2-면에 분리된 Si3N4-표면 층인 것을 특징으로 하는 바이오센서.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019970702298A 1994-10-08 1995-09-30 신호 활성표면에 공유결합으로 고정된 생체물질을 갖는 바이오센서(biosensor with biomaterial covalently fixed on a signal-active surface) KR970706495A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE4436001A DE4436001C2 (de) 1994-10-08 1994-10-08 Biosensor mit auf einer Si¶3¶N¶4¶-Oberfläche fixiertem Enzym
DEP4436001.0 1994-10-08
PCT/DE1995/001371 WO1996011402A1 (de) 1994-10-08 1995-09-30 Biosensor mit auf einer signalaktiven oberfläche kovalent fixiertem biomaterial

Publications (1)

Publication Number Publication Date
KR970706495A true KR970706495A (ko) 1997-11-03

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ID=6530280

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Application Number Title Priority Date Filing Date
KR1019970702298A KR970706495A (ko) 1994-10-08 1995-09-30 신호 활성표면에 공유결합으로 고정된 생체물질을 갖는 바이오센서(biosensor with biomaterial covalently fixed on a signal-active surface)

Country Status (10)

Country Link
EP (1) EP0784791B1 (ko)
JP (1) JPH10506994A (ko)
KR (1) KR970706495A (ko)
CN (1) CN1166874A (ko)
AT (1) ATE209357T1 (ko)
BR (1) BR9509127A (ko)
CA (1) CA2202043A1 (ko)
DE (2) DE4436001C2 (ko)
MX (1) MX9702515A (ko)
WO (1) WO1996011402A1 (ko)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19621165C1 (de) * 1996-05-24 1997-10-02 Karlsruhe Forschzent Verfahren zur Herstellung einer Probe aus immobilisierten Makromolekülen
DE19749216C1 (de) * 1997-11-06 1999-04-15 Forschungszentrum Juelich Gmbh Meßvorrichtung mit Probenzelle zum Nachweis von kleinen Probenmengen in Flüssigkeiten
DE10312296B4 (de) * 2003-03-20 2007-02-15 Forschungszentrum Jülich GmbH Reagenzloser Biosensor zum Nachweis von Nitrilen und Cyaniden
DE10320898A1 (de) * 2003-05-09 2004-12-02 Iongate Biosciences Gmbh Biokompatible Sensorelektrodenanordnung und Verfahren zu deren Herstellung
DE10320899A1 (de) * 2003-05-09 2004-12-02 Iongate Biosciences Gmbh Sensoranordnung, Vorrichtung und Verfahren zur amperometrischen und/oder potentiometrischen, pharmakologischen Wirkort- und/oder Wirkstofftestung
FR2945124B1 (fr) 2009-04-29 2011-07-08 Burkert Werke Gmbh & Co Kg Procede et dispositif de mesure de la concentration d'un analyte dans un liquide echantillon

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8314523D0 (en) * 1983-05-25 1983-06-29 Lowe C R Diagnostic device
CA1316212C (en) * 1987-05-05 1993-04-13 Edward Bakewell Weiler Cycling biochip sensor
US5234820A (en) * 1989-06-30 1993-08-10 Board Of Regents Of The University Of Nebraska Exopeptidase catalyzed site-specific bonding of supports, labels and bioactive agents to proteins
EP0562372B1 (de) * 1992-03-23 1997-11-26 Siemens Aktiengesellschaft Biosensor
CA2064683A1 (en) * 1992-03-26 1993-09-27 Krishna Mohan Rao Kallury Formation of thermostable enzymes with extra-ordinary heat tolerance by immobilization on phospholipid matrices
US5412083A (en) * 1992-04-16 1995-05-02 Northeastern University Carbohydrate heterobifunctional cross-linking reagent

Also Published As

Publication number Publication date
EP0784791B1 (de) 2001-11-21
WO1996011402A1 (de) 1996-04-18
EP0784791A1 (de) 1997-07-23
DE4436001C2 (de) 1996-07-25
CA2202043A1 (en) 1996-04-18
DE4436001A1 (de) 1996-04-11
CN1166874A (zh) 1997-12-03
JPH10506994A (ja) 1998-07-07
BR9509127A (pt) 1997-09-02
ATE209357T1 (de) 2001-12-15
MX9702515A (es) 1998-02-28
DE59509868D1 (de) 2002-01-03

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