KR970062039A - 루신 탈수소효소 활성을 갖는 효소, 이를 코딩하는 뉴클레오타이드 서열 및 효소의 제조 방법 - Google Patents

루신 탈수소효소 활성을 갖는 효소, 이를 코딩하는 뉴클레오타이드 서열 및 효소의 제조 방법 Download PDF

Info

Publication number
KR970062039A
KR970062039A KR1019970005217A KR19970005217A KR970062039A KR 970062039 A KR970062039 A KR 970062039A KR 1019970005217 A KR1019970005217 A KR 1019970005217A KR 19970005217 A KR19970005217 A KR 19970005217A KR 970062039 A KR970062039 A KR 970062039A
Authority
KR
South Korea
Prior art keywords
acid
oxo
sequence
escherichia coli
enzyme
Prior art date
Application number
KR1019970005217A
Other languages
English (en)
Inventor
탄야 스토얀
마리아-레기나 쿨라
Original Assignee
베버 볼프강. 펠트만
데구사 아크티엔게젤샤프트
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 베버 볼프강. 펠트만, 데구사 아크티엔게젤샤프트 filed Critical 베버 볼프강. 펠트만
Publication of KR970062039A publication Critical patent/KR970062039A/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/834—Bacillus cereus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

본 발명은 루신 탈수소효소 (LeuDH) 활성을 갖는 효소, 이를 코딩하는 바실루스 세레우스(Bacillus cereus) 뉴클레오타이드 서열, 이 서열을 함유하는 플라스미드를 사용한 이. 콜라이(E. coli) 미생물의 형질전환 및 효소의 제조방법에 관한 것이다.

Description

루신 탈수소효소 활성을 갖는 효소, 이를 코딩하는 뉴클레오타이드 서열 및 효소의 제조 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 pUC18에 포함된 바실루스 세레우스(Bacillus cereus)로 부터의 루신 탈수소효소(LeuDH)에 대한 유전자를 나타낸다.
제2도는 비. 세레우스로부터의 LeuDH에 대한 유전자의 서열분석을 나타낸다(Sma Ⅰ/XbaⅠ 단편을 포함하는 pleu2).

Claims (9)

  1. 루신 탈수소효소(LeuDH) 활성을 갖는 효소를 코딩하고; (a) 서열 목록에 도시된 뉴클레오타이드 서열, (b) 유전 코드의 축퇴범위에 속하는 (a)의 서열에 상응하는 뉴클레타이드 서열, 또는 (c) 서열(a), 서열(b) 또는 서열(a)와 (b)모두의 서열과 하이브리드화하는 뉴클레오타이드 서열을 포함함을 특징으로 하는 바실루스 세레우스(Bacilus cereus) DNA 서열.
  2. 제1항에 따른 뉴클레타이트 서열이 삽입되어 있는, 에스케리키아 콜라이(Escherichia coli)에서 자가 복제할 수 있는 백터.
  3. 제2항에 있어서, 플라스미드 pUC18의 DNA 서열과 제1항의 DNA 서열을 함유하고, 에스케리키아 콜라이 XL1 blue 중의 pLeu 2로서 기탁번호 DSM 10441로 기탁되어 있으며, 제2도의 제한 지도로 특정지워짐을 특징으로 하는 벡터.
  4. 제2항 또는 제3항에 따른 벡터를 함유하는 LeuDH에 대해 고 생성능을 갖는 에스케리키아 콜라이.
  5. 제4항에 있어서, 에스케리아 콜라이 XL1 blue로서 기탁번호 10441로 기탁되어 있는 에스케리키아 콜라이.
  6. (a) 제1항에 따른 바실루드 세레우스로부터 유도된 뉴클레오타이드 서열 및 (b) 에스케리키아 콜라이내에서 자가 복제되는 플라스미드 DNA를 함유하는 재조합체 DNA를 벡터의 형태로 에스케리키아 콜라이에 삽입시킨 후, 생성된 형질전환체를 적합한 배지에서 발효시키고, Leu-DH를 미생물로부터 분리시킴을 특징으로 하는 , 루신 탈수소효소(LeuDH)의 제조 방법.
  7. 제6항에 있어서, 제4항 또는 제5항에 따른 미생물이 사용되는 방법.
  8. 상응하는 α-케토산으로부터 비-단백질원성 L-아미노산을 제조하기 위한, 제6항 또는 제7항에 따라 수득된 LeuDH 활성을 갖는 효소의 용도.
  9. 사용되는 α-케토산이 2-옥소-4-메틸펜타노산, 2-옥소-3-메틸펜타노산, 2-옥소-3-메틸부타노산, 2-옥소펜타노산, 2-옥소-4-메틸헥사노산, 2-옥소부타노산, 2-옥소-3, 3-디메틸부타노산, 2-옥소헥사노산, 2-옥소-4, 4-디멜틸펜타노산, 2-옥소-3,3-디메틸펜타노산, 2-옥소-4-에틸헥사노산, 2-옥소-5, 5-디멜틸헥사노산, 2-옥소-3-사이클로헥실프로파노산 및 2-옥소-4, 4-디멜틸헥사노산임을 특징으로 하는 제6항 또는 제8항에 따른 효소의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019970005217A 1996-02-22 1997-02-21 루신 탈수소효소 활성을 갖는 효소, 이를 코딩하는 뉴클레오타이드 서열 및 효소의 제조 방법 KR970062039A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19606494.5 1996-02-22
DE19606494A DE19606494A1 (de) 1996-02-22 1996-02-22 Enzym mit LeuDH-Aktivität, dafür codierende Nukleotid-Sequenz und Verfahren zur Herstellung des Enzyms

Publications (1)

Publication Number Publication Date
KR970062039A true KR970062039A (ko) 1997-09-12

Family

ID=7786032

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019970005217A KR970062039A (ko) 1996-02-22 1997-02-21 루신 탈수소효소 활성을 갖는 효소, 이를 코딩하는 뉴클레오타이드 서열 및 효소의 제조 방법

Country Status (7)

Country Link
US (1) US5854035A (ko)
EP (1) EP0792933A3 (ko)
JP (1) JPH09224688A (ko)
KR (1) KR970062039A (ko)
CA (1) CA2198160A1 (ko)
DE (1) DE19606494A1 (ko)
SG (1) SG74579A1 (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7202070B2 (en) * 2000-10-31 2007-04-10 Biocatalytics, Inc. Method for reductive amination of a ketone using a mutated enzyme
DE102004008445A1 (de) 2004-02-19 2005-09-08 Degussa Ag Verfahren zur Herstellung von L-Aminosäuren aus D-Aminosäuren
WO2006015885A1 (en) * 2004-08-13 2006-02-16 University College Dublin, National University Of Ireland, Dublin Amino acid dehydrogenase-derived biocatalysts
WO2011100265A2 (en) 2010-02-10 2011-08-18 Codexis, Inc. Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system
US9688967B2 (en) 2014-12-05 2017-06-27 Synlogic, Inc. Bacteria engineered to treat diseases associated with hyperammonemia

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54119290A (en) * 1978-03-09 1979-09-17 Mitsui Toatsu Chemicals Reagent for measuring quantity of leucine aminopeptidase
JP2615090B2 (ja) * 1987-11-16 1997-05-28 ユニチカ株式会社 プラスミド及びそれで形質転換されたエシェリチア・コリ

Also Published As

Publication number Publication date
JPH09224688A (ja) 1997-09-02
DE19606494A1 (de) 1997-08-28
EP0792933A2 (de) 1997-09-03
SG74579A1 (en) 2004-02-27
EP0792933A3 (de) 1999-03-03
US5854035A (en) 1998-12-29
CA2198160A1 (en) 1997-08-22

Similar Documents

Publication Publication Date Title
Verhaert et al. Molecular cloning and analysis of the gene encoding the thermostable penicillin G acylase from Alcaligenes faecalis
EP1602722B1 (en) Process for producing microbial transglutaminase
US5888785A (en) Method for using hydratase or a hydratase-amidase fusion for stereospecifically bioconverting certain racemic nitriles to the corresponding enatiomeric R--or S-amide or s-carboxylic acid
CN106795485A (zh) 生产o‑磷酸丝氨酸的微生物和利用其生产o‑磷酸丝氨酸或l‑半胱氨酸的方法
US20070190604A1 (en) Novel Gene Products From Bacillus Licheniformis Forming Or Decomposing Polyamino Acids And Improved Biotechnological Production Methods Based Thereon
WO2002070717A2 (en) Nitrile hydratase and a method for producing amides
CA2327266A1 (en) Pesticidal toxins and nucleotide sequences which encode these toxins
Wilms et al. Cloning, nucleotide sequence and expression of a new LN-carbamoylase gene from Arthrobacter aurescens DSM 3747 in E. coli
US5705379A (en) Nucleotide sequences encoding a thermostable alkaline protease
JP3408737B2 (ja) ニトリルヒドラターゼの活性化に関与するタンパク質及びそれをコードする遺伝子
KR970062039A (ko) 루신 탈수소효소 활성을 갖는 효소, 이를 코딩하는 뉴클레오타이드 서열 및 효소의 제조 방법
EP0780471A3 (en) Novel nitrilase gene
Arima et al. Gene cloning and overproduction of an aminopeptidase from Streptomyces septatus TH-2, and comparison with a calcium-activated enzyme from Streptomyces griseus
JPH1189575A (ja) 微生物を用いたアミド化合物の製造方法
JPH0638766A (ja) ウリカーゼ遺伝子およびウリカーゼの製造法
US7195900B2 (en) Cloning and expression of D-amino acid oxidase from arthrobacter protophormiae
US6890746B1 (en) Gene participating in the production of homo-glutamic acid and utilization thereof
JP2004526445A (ja) Ａｒｔｈｒｏｂａｃｔｅｒｃｒｙｓｔａｌｌｏｐｏｉｅｔｅｓ（アリスロバクテリアクリスタロポイテス）ＤＳＭ２０１１７株由来のＤ−カルバモイラーゼ
Noonpakdee et al. Molecular cloning, DNA sequencing and expression of catalase gene of Lactobacillus sake SR 911 in Escherichia coli UM 2
JP4397088B2 (ja) 還元酵素をコードする遺伝子
Rossi et al. Enzymes from extreme thermophilic bacteria as special catalysts: studies on a β-galactosidase from Sulfolobus solfataricus
KR970021294A (ko) 유기산 생성이 억제된 대장균 변이주
JPH06113840A (ja) ザルコシンオキシダーゼ活性を有する蛋白質及びその遺伝情報を有するｄｎａ、並びにザルコシンオキシダーゼの製造法
JPH11225769A (ja) 糖類変換酵素をコードするｄｎａ、それを含む組換えｄｎａ並びに形質転換体
CA2407059A1 (en) Novel (r)-2-hydroxy-3-phenylpropionic acid (d-phenyllactic acid) dehydrogenase and gene encoding the same

Legal Events

Date Code Title Description
N231 Notification of change of applicant
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid