KR970043066A - 본취용 역상 고압 액체 크로마토그래피(Prep-HPLC)를 이용한 반코마이신의 정제방법 - Google Patents

본취용 역상 고압 액체 크로마토그래피(Prep-HPLC)를 이용한 반코마이신의 정제방법 Download PDF

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KR970043066A
KR970043066A KR1019960058825A KR19960058825A KR970043066A KR 970043066 A KR970043066 A KR 970043066A KR 1019960058825 A KR1019960058825 A KR 1019960058825A KR 19960058825 A KR19960058825 A KR 19960058825A KR 970043066 A KR970043066 A KR 970043066A
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KR
South Korea
Prior art keywords
vancomycin
exchange resin
liquid chromatography
high pressure
pressure liquid
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KR1019960058825A
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English (en)
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KR100419707B1 (ko
Inventor
유원일
강성구
이광섭
황수연
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손경식
제일제당 주식회사
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Publication of KR970043066A publication Critical patent/KR970043066A/ko
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Publication of KR100419707B1 publication Critical patent/KR100419707B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K9/00—Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof
    • C07K9/006—Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof the peptide sequence being part of a ring structure
    • C07K9/008—Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof the peptide sequence being part of a ring structure directly attached to a hetero atom of the saccharide radical, e.g. actaplanin, avoparcin, ristomycin, vancomycin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

본 발명은 반코마이신을 함유하는 미생물 발효배양액을 pH2.0 내지 4.0으로 조절하여 강산성 양이온 교환수지, 강염기성 음이온 교환수지 및 다공성(porous)의 강산성 양이온 교환수지를 연속 통과시키고 활성 알루미나(Alumina activated)수지를 통과시킨 다음, C-8 역상 칼럼을 사용하는 분취용 고합 액체 크로마토그래피(Prep-HPLC)에 적용함으로써 반코마이신을 고수율로 신속하게 분리, 회수함을 특징으로하는 반코마이신의 정제방법에 관한 것이다.

Description

본취용 역상 고압 액체 크로마토그래피(Prep-HPLC)를 이용한 반코마이신의 정제방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 2도는 C-8역상 칼럼을 사용한 Prep-HPLC 로부터 분취한 반코마이신을 HPLC로 분석한 결과도이다.(USP 분석법).

Claims (5)

  1. 반코마이신을 함유하는 미생물 발효배양액을 pH 2.0내지 4.0으로 조절하여 강산성 양이온 교환수지, 강염기성 음이온 교환수지 및 다공성의 강산성 양이온 교환수지를 연속 통과시키고 활성 알루미나 수지를 통과시킨 다음, C-8 역상 칼럼을 사용하는 분취용 고압 액체 크로마토그래피에 적용함으로써 반코마이신을 분리, 정제하는 방법.
  2. 제 1항에 있어서, 분취용 고압 액체 크로마토그래피를 수행함에 있어 이동상으로 10 내지 20% 메탄올을 함유한 10내지 100mM 인산암모늄 완충용액 또는 염화암모늄 완충용액을 사용하는 방법.
  3. 제 2항에 있어서, 완충용액의 pH가 2.0 내지 3.0 인 방법.
  4. 제 1항에 있어서, C-8 역상 칼럼이 Kromasil 100-5-C8 인 방법.
  5. 제 1항에 있어서, 미생물이 아미콜라톱시스 오리엔탈리스(Amycolatopsis orientalis)인 방법.
KR1019960058825A 1995-12-29 1996-11-28 분취용 역상 고압 액체 크로마토그래피를 이용한반코마이신의 정제방법 KR100419707B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR19950067288 1995-12-29
KR101995067288 1995-12-29
KR1019950067288 1995-12-29

Publications (2)

Publication Number Publication Date
KR970043066A true KR970043066A (ko) 1997-07-26
KR100419707B1 KR100419707B1 (ko) 2004-06-12

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KR1019960058825A KR100419707B1 (ko) 1995-12-29 1996-11-28 분취용 역상 고압 액체 크로마토그래피를 이용한반코마이신의 정제방법

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080075979A (ko) * 2007-02-14 2008-08-20 주식회사 바이오앤진 아미코라톱시스 오리엔탈리스 변이주 및 이를 이용한반코마이신 염산염의 제조방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2577133B2 (ja) * 1989-11-27 1997-01-29 スティフティング フォール ド テフニスヘ ウェッテンスハッペン 船舶のプロペラ
KR950009201B1 (ko) * 1991-12-26 1995-08-16 제일제당주식회사 이온 교환수지 및 알루미나 수지를 이용한 반코마이신의 정제
KR100795289B1 (ko) * 2006-01-17 2008-01-15 한국델파이주식회사 자동차용 트라이포드 등속 조인트

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