KR970006500A - Method for Purifying Gamma Interferon Expressed from Recombinant Yeast - Google Patents

Method for Purifying Gamma Interferon Expressed from Recombinant Yeast Download PDF

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Publication number
KR970006500A
KR970006500A KR1019950021489A KR19950021489A KR970006500A KR 970006500 A KR970006500 A KR 970006500A KR 1019950021489 A KR1019950021489 A KR 1019950021489A KR 19950021489 A KR19950021489 A KR 19950021489A KR 970006500 A KR970006500 A KR 970006500A
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South Korea
Prior art keywords
gamma interferon
recombinant yeast
cation exchange
cultured
dialysis
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KR1019950021489A
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Korean (ko)
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KR100374307B1 (en
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박천길
한규범
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성재갑
주식회사 Lg 화학
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Priority to KR1019950021489A priority Critical patent/KR100374307B1/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/57—IFN-gamma
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 재조합 효모로부터 발현된 감마 인터페론을 정제하는 방법에 관한 것으로, 감마 인터페론을 발현하는 형질전환된 재조합 효모세포를 저온에서 배양하고, 배양된 효모 세포를 파쇄하여 불용성 물질을 제거하고, 가용성 부분을 양이온 교환 크로마토그래피 및 겔 여과크로마토그래피하고 투석하는 단계를 포함하는 방법에 의하면 고순도 및 고역가의 감마 인터페론을 얻을 수 있다.The present invention relates to a method for purifying gamma interferon expressed from recombinant yeast, wherein the transformed recombinant yeast expressing gamma interferon is cultured at low temperature, and the cultured yeast cells are crushed to remove insoluble substances, and the soluble portion. According to the method comprising the step of cation exchange chromatography and gel filtration chromatography and dialysis can obtain a high purity and high titer gamma interferon.

Description

재조합 효모로부터 발현된 감마 인터페론의 정제 방법Method for Purifying Gamma Interferon Expressed from Recombinant Yeast

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (5)

감마 인터페론을 발현하는 형질전환된 재조합 효모세포를 저온에서 배양하고, 배양된 효모 세포를 파쇄하여 불용성 물질을 제거하고, 가용성 부분을 양이온 교환 크로마토그래피 및 겔 여과 크로마토그래피하고 투석하는 단계를 포함하는 재조합 효모로부터 발현된 감마 인터페론의 정제 방법.Recombination comprising culturing the transformed recombinant yeast expressing gamma interferon at low temperature, crushing the cultured yeast cells to remove insoluble material, and subjecting the soluble portion to cation exchange chromatography and gel filtration chromatography and dialysis Method for Purifying Gamma Interferon Expressed from Yeast. 제1항에 있어서, 상기 재조합 효모세포를 15±2℃에서 배양하는 것을 특징으로 하는 방법.The method of claim 1, wherein the recombinant yeast cells are cultured at 15 ± 2 ℃. 제1항에 있어서, 상기 양이온 교환 크로마토그래피에서 양이온 교환 수지로 SP-세파덱스 또는 CM-세파덱스를 사용하고, 트리스를 포함하는 pH 6.5 내지 8.5의 완충용액중 0.2 내지 0.8M의 선형농도 구배의 염화나트륨 용액으로 용출시키는 것을 특징으로 하는 방법.The method according to claim 1, wherein the cation exchange chromatography uses SP-sefadex or CM-sefadex as the cation exchange resin, and has a linear concentration gradient of 0.2 to 0.8 M in a buffer of pH 6.5 to 8.5 containing Tris. Eluting with sodium chloride solution. 제1항에 있어서, 상기 겔 여과 크로마토그래피에서 수지로는 세파크릴-100,200 또는 300을 사용하고, pH 7.5 내지 8.5의 완충용액으로 용출시키는 것을 특징으로 하는 방법.The method of claim 1, wherein in the gel filtration chromatography, Sephacryl-100,200 or 300 is used as the resin, and the eluting with a buffer solution of pH 7.5 to 8.5. 제1항에 있어서, 상기 투석을 2.5%의 덱스트로스 수용액에서 실시하는 것을 특징으로 하는 방법.The method of claim 1, wherein said dialysis is carried out in an aqueous 2.5% dextrose solution. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950021489A 1995-07-21 1995-07-21 A purification method of gamma interferon from recombinant yeast KR100374307B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950021489A KR100374307B1 (en) 1995-07-21 1995-07-21 A purification method of gamma interferon from recombinant yeast

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950021489A KR100374307B1 (en) 1995-07-21 1995-07-21 A purification method of gamma interferon from recombinant yeast

Publications (2)

Publication Number Publication Date
KR970006500A true KR970006500A (en) 1997-02-21
KR100374307B1 KR100374307B1 (en) 2003-07-07

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ID=37416773

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KR1019950021489A KR100374307B1 (en) 1995-07-21 1995-07-21 A purification method of gamma interferon from recombinant yeast

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Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5489011A (en) * 1977-12-24 1979-07-14 Green Cross Corp:The Preparation of interferon
ZA843275B (en) * 1983-05-05 1984-12-24 Hoffmann La Roche Novel vectors for interferon expression
DE3379506D1 (en) * 1983-08-22 1989-05-03 Kyowa Hakko Kogyo Kk Process for producing peptide
IT1246296B (en) * 1990-07-25 1994-11-17 Sclavo Spa PROCEDURE FOR EXTRACTION AND PURIFICATION OF THE RECOMBINANT HUMAN INTERPHONE RANGE
JP3523293B2 (en) * 1993-07-19 2004-04-26 住友製薬株式会社 Method for enhancing interferon production

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