KR970000190A - 2D sensor for measuring neuronal activity and measuring device using the same - Google Patents

2D sensor for measuring neuronal activity and measuring device using the same Download PDF

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Publication number
KR970000190A
KR970000190A KR1019960022231A KR19960022231A KR970000190A KR 970000190 A KR970000190 A KR 970000190A KR 1019960022231 A KR1019960022231 A KR 1019960022231A KR 19960022231 A KR19960022231 A KR 19960022231A KR 970000190 A KR970000190 A KR 970000190A
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South Korea
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cell activity
dimensional sensor
sensor
light source
fence
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KR1019960022231A
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Korean (ko)
Inventor
히로카즈 스기하라
마코토 타케타니
아키히토 카메이
히로시 이와사키
Original Assignee
모리시타 요이찌
마쯔시다덴기산교 가부시기가이샤
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Publication of KR970000190A publication Critical patent/KR970000190A/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/305—Electrodes, e.g. test electrodes; Half-cells optically transparent or photoresponsive electrodes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/806—Electrical property or magnetic property

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement Of Current Or Voltage (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

A two-dimensional sensor (1) is described including a substrate having a Si layer, a SiO2 layer and a Si3N4 layer. On the surface of the Si back side, a thin film is formed by vapor deposition for making an effect electrode. On the surface of the Si3N4 front side, a fence is attached for containing a sample cell (2), culture medium and a reference electrode. This sensor (1) is placed in an incubator (3) and a bias voltage is applied between the effect and reference electrodes. When a high frequency modulated laser beam irradiates a spot of the back side of the sensor substrate, a signal of AC photocurrent is obtained from the effect electrode. This signal corresponds to a potential alteration due to the cell activity substantially at the spot. The signal is processed in a computer (10). Therefore, the beam spot size and location, corresponding to the size and the location of the measurement electrode, can be adjusted easily by focusing or moving the laser beam. <IMAGE>

Description

신경세포활동측정용 2차원센서 및 이것을 사용한 측정장치2D sensor for measuring neuronal activity and measuring device using the same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명의 실시예에 관한 세포활동측정장치의 구성도.1 is a block diagram of a cell activity measuring apparatus according to an embodiment of the present invention.

Claims (7)

Si, SiO2, 및 Si3N4의 3층으로 이루어진 센서기판 Si쪽의 이면에 작용전극용 박막이 증착되고, Si3N4쪽의 표면에 시료인 세포, 배양액, 및 참조전극을 넣는 울타리가 형성되고, 그 울타리의 내부에 놓인 세포의 활동에 의해서 발생하는 전위변화의 평면분포증, 광이 조사된 부분의 전위변화에 주로 대응하는 신호가 상기 작용전극으로부터 꺼내지는 것을 특징으로 하는 세포활동측정용 2차원센서.A thin film for the working electrode is deposited on the back side of the sensor substrate Si composed of three layers of Si, SiO 2 , and Si 3 N 4 , and a fence into which the sample cells, the culture solution, and the reference electrode are placed on the surface of the Si 3 N 4 side. Cell activity characterized in that the planar distribution of the potential change caused by the activity of the cells placed inside the fence and the signal mainly corresponding to the potential change of the irradiated part are taken out of the working electrode Two-dimensional sensor for measurement. 청구범위 제1항에 기재된 세포활동측정용 2차원센서와, 그 이면에 레이저빔을 조사하는 레이저광원과, 상기 세포활동측정용 2차원센서의 이면쪽의 작용전극과 표면쪽의 울타리내에 넣어진 참조전극과의 사이에 직류바이어스전압을 인가하는 직류전원과, 상기 양전극간에 얻어지는 신호를 처리하는 처리수단을 구비하고 있는 것을 특징으로 하는 세포활동측정장치.The two-dimensional sensor for measuring cell activity according to claim 1, a laser light source for irradiating a laser beam on the back side thereof, a working electrode on the back side of the two-dimensional sensor for cell activity measurement, and placed in a fence on the surface side. And a DC power supply for applying a DC bias voltage between the reference electrode and processing means for processing a signal obtained between the two electrodes. 제2항에 있어서, 상기 세포활동측정용 2차원센서의 울타리내에 놓인 세포의 배양환경을 유지하는 배양유지수단이 구비되어 있는 것을 특징으로 하는 세포활동측정장치.The cell activity measuring apparatus according to claim 2, further comprising a culture holding means for maintaining a culture environment of cells placed in a fence of the two-dimensional sensor for measuring cell activity. 제2항에 있어서, 상기 레이저광원을 고주파구동해서 고주파변조광을 조사시키는 광원구동수단이 설치되고, 상기 처리수단은, 상기 양전극간에 흐르는 광교류전류의 진폭의 변화를 검출하도록 구성되어 있는 것을 특징으로 하는 세포활동측정장치.The light source driving means for irradiating high frequency modulated light by driving the laser light source at high frequency is provided, and the processing means is configured to detect a change in amplitude of the light alternating current flowing between the two electrodes. Cell activity measuring device. 제2항에 있어서, 상기 레이저광원으로부터 조사되는 레이저빔을 상기 세포활동측정용 2차원센서의 이면의 소정범위내에서 주사하는 수단이 구비되어 있는 것을 특징으로 하는 세포활동측정장치.The cell activity measuring apparatus according to claim 2, further comprising means for scanning a laser beam irradiated from the laser light source within a predetermined range of the back surface of the two-dimensional sensor for measuring cell activity. 제2항에 있어서, 상기 레이저광원으로서, 상기 세포활동측정용 2차원센서의 이면에 대해서 거의 수직으로 레이저광을 조사하는 복수의 레이저가 2차원매트릭스형상으로 배열되어 이루어진 레이저 어레이가 구비되어 있는 것을 특징으로 하는 세포활동측정장치.3. A laser array as claimed in claim 2, wherein said laser light source is provided with a laser array in which a plurality of lasers for irradiating laser light substantially perpendicular to a back surface of said two-dimensional sensor for cell activity measurement are arranged in a two-dimensional matrix shape. Cell activity measuring device characterized in that. 제2항에 있어서, 상기 세포활동측정용 2차원센서의 수평방향에 있어서의 위치를 제어하는 XY스테이지가 구비되어 있는 것을 특징으로 하는 세포활동측정장치.The cell activity measuring apparatus according to claim 2, wherein an XY stage for controlling the position in the horizontal direction of the two-dimensional sensor for cell activity measurement is provided. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019960022231A 1995-06-20 1996-06-19 2D sensor for measuring neuronal activity and measuring device using the same KR970000190A (en)

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JP95-153343 1995-06-20
JP7153343A JP2930181B2 (en) 1995-06-20 1995-06-20 Two-dimensional sensor for measuring nerve cell activity and measuring device using the same

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CN (1) CN1156825A (en)
AT (1) ATE211823T1 (en)
CA (1) CA2179459A1 (en)
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Publication number Publication date
JP2930181B2 (en) 1999-08-03
EP0750195A1 (en) 1996-12-27
CN1156825A (en) 1997-08-13
EP0750195B1 (en) 2002-01-09
DE69618390T2 (en) 2002-09-26
TW324062B (en) 1998-01-01
US6288527B1 (en) 2001-09-11
ATE211823T1 (en) 2002-01-15
CA2179459A1 (en) 1996-12-21
JPH095289A (en) 1997-01-10
DE69618390D1 (en) 2002-02-14

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