KR960023071A - Method for preparing L-leucine by fermentation - Google Patents

Method for preparing L-leucine by fermentation Download PDF

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Publication number
KR960023071A
KR960023071A KR1019940035557A KR19940035557A KR960023071A KR 960023071 A KR960023071 A KR 960023071A KR 1019940035557 A KR1019940035557 A KR 1019940035557A KR 19940035557 A KR19940035557 A KR 19940035557A KR 960023071 A KR960023071 A KR 960023071A
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South Korea
Prior art keywords
leucine
fermentation
producing
isoleucine
cysteine
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KR1019940035557A
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Korean (ko)
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KR0144641B1 (en
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한재춘
전준현
유영수
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이삼우
주식회사 세 원
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Priority to KR1019940035557A priority Critical patent/KR0144641B1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 발효에 의한 L-로이신의 제조방법으로 더욱 자세하게는 L-이소로이신 및 L-시스테인 요구 변이주의 호기적 배양에 의해 L-로이신을 제조하는 방법에 관한 것으로 L-로이신 생산에 이용해 오던 L-이소로이신 요구주인 코리네박테리움 속 동아 MS-03178(KFCC-10011)을 친주로 하여 통상의 변이처리방법에 의하여 획득한 변이주중 L-이소로이신 및 L-시스테인을 동시에 요구하는 변이주인 코리네박테리움 동아 MS-03381(KCTC-8508P)를 당류를 주원료로 하는 배지에 호기적으로 배양하여 L-로이신을 배지내에 축척시켜 L-로이신을 제조하는 방법으로서 본 변이주가 다량의 L-로이신을 배지내에 축적시킴으로 수율이 양호하여 공업화에 유용한 L-로이신의 제조방법이다.The present invention relates to a method for producing L-leucine by fermentation, and more particularly, to a method for producing L-leucine by aerobic culture of L-isoleucine and L-cysteine demanding mutant strains. Coryne, a mutant strain requiring L-isoleucine and L-cysteine simultaneously, among the mutant strains obtained by conventional mutation processing methods using Dong-A MS-03178 (KFCC-10011) as a parent strain of isoleucine. Bacterium Donga MS-03381 (KCTC-8508P) was aerobically cultured in a medium containing saccharides, and L-leucine was accumulated in the medium to produce L-leucine. It is a manufacturing method of L- leucine useful in industrialization with a high yield by accumulating inside.

Description

발효에 의한 L-로이신의 제조 방법Method for producing L-leucine by fermentation

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (5)

발효에 의한 L-로이신을 제조함에 있어서, 생육에 L-이소로이신 및 L-시스테인을 동시에 요구하는 변이주인 코리네박테리움속 동아 MS-03381(KCTC-8508P)를 이용하여 L-로이신을 제조함을 특징으로 하는 발효에 의한 L-로이신의 제조방법.In the production of L-leucine by fermentation, L-leucine is prepared using a variant strain Corynebacterium sinus MS-03381 (KCTC-8508P), which requires both L-isoleucine and L-cysteine for growth. Method for producing L- leucine by fermentation characterized in that. 제1항에 있어서, 변이주인 코리네박테리움속 동아 MS-03381(KCTC-8508P)를 당질을 주원료로 하는 발효배지에서 호기적으로 배양함을 특징으로 하는 발효에 의한 L-로이신의 제조방법.The method for producing L-leucine by fermentation according to claim 1, wherein the mutant strain Corynebacterium Dong-a MS-03381 (KCTC-8508P) is cultivated aerobicly in a fermentation medium containing sugar as a main ingredient. 제1항 또는 제2항에 있어서, 발효배지에 L-이소로이신을 500㎍/㎖, L-시스테인을 100∼500㎍/㎖ 첨가함을 특징으로 하는 발효에 의한 L-로이신의 제조방법.The method for producing L-leucine by fermentation according to claim 1 or 2, wherein 500 µg / ml of L-isoleucine and 100-500 µg / ml of L-cysteine are added to the fermentation medium. 제1항 내지 제3항 중 어느 1항에 있어서, pH를 6.8∼7.0으로 유지시킴을 특징으로 하는 발효에 의한 L-로이신의 제조방법.The method for producing L-leucine by fermentation according to any one of claims 1 to 3, wherein the pH is maintained at 6.8 to 7.0. 제1항 내지 제4항 중 어느 1항에 있어서, 통기량을 0.5∼1.0vvm으로 유지하면서 배양함을 특징으로 하는 L-로이신의 제조방법.The method for producing L-leucine according to any one of claims 1 to 4, wherein the culture is carried out while maintaining the aeration amount at 0.5 to 1.0 vvm. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940035557A 1994-12-21 1994-12-21 Preparation process of l-leucine by fermentaion KR0144641B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940035557A KR0144641B1 (en) 1994-12-21 1994-12-21 Preparation process of l-leucine by fermentaion

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940035557A KR0144641B1 (en) 1994-12-21 1994-12-21 Preparation process of l-leucine by fermentaion

Publications (2)

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KR960023071A true KR960023071A (en) 1996-07-18
KR0144641B1 KR0144641B1 (en) 1998-07-15

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