KR950704353A - Hepatitis C Viral (HCV) Non-Struct-3 Peptides, Antibodies To It, and Methods for Detecting HCV (HCV) NON-STRUCTURAC-3 PEPTIDES DETECTION OF HCV - Google Patents

Hepatitis C Viral (HCV) Non-Struct-3 Peptides, Antibodies To It, and Methods for Detecting HCV (HCV) NON-STRUCTURAC-3 PEPTIDES DETECTION OF HCV

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Publication number
KR950704353A
KR950704353A KR1019950702373A KR19950702373A KR950704353A KR 950704353 A KR950704353 A KR 950704353A KR 1019950702373 A KR1019950702373 A KR 1019950702373A KR 19950702373 A KR19950702373 A KR 19950702373A KR 950704353 A KR950704353 A KR 950704353A
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KR
South Korea
Prior art keywords
hcv
peptide
detecting
hepatitis
test fluid
Prior art date
Application number
KR1019950702373A
Other languages
Korean (ko)
Inventor
비난드 요한네스 안토니우스 하베츠
피에테르 야콥 보엔데르
Original Assignee
에프.쥐.엠, 헤르만스 페.카.브론스
악조 노벨 엔.브이.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 에프.쥐.엠, 헤르만스 페.카.브론스, 악조 노벨 엔.브이. filed Critical 에프.쥐.엠, 헤르만스 페.카.브론스
Publication of KR950704353A publication Critical patent/KR950704353A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C07K14/02—Hepadnaviridae, e.g. hepatitis B virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1081—Togaviridae, e.g. flavivirus, rubella virus, hog cholera virus
    • C07K16/109—Hepatitis C virus; Hepatitis G virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
    • G01N33/5767—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis non-A, non-B hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

본 발명은 HCV에 대해 유도된 항체와 면역화학적으로 반응성인 펨티드에 관한 것이다. 본 발명에 따른 바람직한 펩티드는 NS-3단백질의 HCV특이성 에피토프를 함유한다. 추가로 본 발명은 HCV의 NS-3 단백질과 특이정이로 반응성인 항체에 관한 것이다. HCV또는 HCV 항체의 검출방법 및 NS-3항원과 특이적으로 반응성인 항체의 검출방법 또는 본 발명의 일부이다.The present invention relates to femtides immunochemically reactive with antibodies induced against HCV. Preferred peptides according to the invention contain HCV-specific epitopes of the NS-3 protein. The present invention further relates to antibodies which are specifically reactive with NS-3 protein of HCV. Methods of detecting HCV or HCV antibodies and methods of detecting antibodies specifically reactive with NS-3 antigens or as part of the present invention.

Description

씨(C)형 간염 비루스(HCV) 비-구조-3 펩티드, 그에 대한 항체 및 에이취씨브이(HCV)의 검출 방법 (HEPATITIS C VIRUS(HCV) NON-STRUCTURAC-3PEPTIDES, ANTIBODIES TH드RETO AND METHODS FOR THE DETECTION OF HCV)Hepatitis C Virus (HCV) Non-Struct-3 Peptides, Antibodies To It, and Methods for Detection of HCV (HEPATITIS C VIRUS (HCV) NON-STRUCTURAC-3PEPTIDES, THE DETECTION OF HCV

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (12)

C형 간염 비루스의 NS-3 항원의 KCV 특이성 에피토프를 함유하는 펩티드.Peptides containing KCV specific epitopes of NS-3 antigen of hepatitis C virus. 제1항에 있어서, 면역화학적으로 HCV-항체들과 반응성인 서열동정번호 ; 6, 7, 8, 9 및 10에 묘사한 서열군 및 이의 조합 또는 상기 서열군의 단편 또는 상기 서열군의 유사체로 부터 선택되는 아미노산 서열을 함유하는 펩티드.The method of claim 1, further comprising: SEQ ID NO: immunochemically reactive with HCV-antibodies; A peptide containing an amino acid sequence selected from the group of sequences depicted in 6, 7, 8, 9 and 10 and combinations thereof or fragments of the group or analogs of the group. 제1항 또는 제2항의 펩티드를 함유하는 면역화학적 시약.An immunochemical reagent containing the peptide of claim 1 or 2. 제1항 또는 제2항의 펩티드클 암호화하는 핵산 서열.A nucleic acid sequence encoding the peptide of claim 1. 제4항에 있어서, 서열동정번호 : 1, 2, 3, 4 및 5에 도시한 DNA서열중 적어도 일부분을 함유하는 핵산 서열.The nucleic acid sequence of claim 4 containing at least a portion of the DNA sequences shown as SEQ ID NOs: 1, 2, 3, 4, and 5. 6. 제4항 또는 제5항의 핵산 서열을 함유하는 재조합 벡터 분자.A recombinant vector molecule containing the nucleic acid sequence of claim 4. 제6항의 벡터를 사용하여 형질전환된 숙주세포.A host cell transformed using the vector of claim 6. 제1항 또는 제2항의 단일클론 항체를 함유하는 면역 진단 시약.An immunodiagnostic reagent containing the monoclonal antibody of claim 1. ** 테스트 유체내에서 HCV에 대해 유도된 항체의 검출방법으로서, 이때 제1항 또는 제2항의 펩티드는 테스트 유체와 접촉하며, 상기 펩티드와 항체 사이에서 형성된 면역 복합체의 존재가 테스트 유체내에서 검출되는 방법.A method of detecting an antibody directed against HCV in a test fluid, wherein the peptide of claim 1 or 2 is in contact with the test fluid and the presence of an immune complex formed between the peptide and the antibody is detected in the test fluid. . 제8항의 단일클론 항체를 사용하여 샘플을 접촉시키고, 상기 단일클론 항체와 C형 간염 항원 사이에서 형성된 면역 복합체를 검출함을 포함하여, 샘플내에서 C형 간염 비루스를 검출하는 방법.A method of detecting hepatitis C virus in a sample comprising contacting a sample using the monoclonal antibody of claim 8 and detecting an immune complex formed between said monoclonal antibody and hepatitis C antigen. 테스트 유체내에서 C형 간염 비루스의 NS-3 단백질의 HCV 특이성 에피토프에 대해 유도된 항체를 검출하는 방법으로서, 이때 제1항 또는 제2항의 펩티드가 테스트 유체와 접촉하고, 상기 펩티드와 항체 사이에서 형성된 면역 복합체의 존재가 테스트 유체내에서 검출되는 방법.A method for detecting an antibody directed against HCV specific epitopes of NS-3 protein of hepatitis C virus in a test fluid, wherein the peptide of claim 1 or 2 is in contact with the test fluid and between the peptide and the antibody. The presence of the formed immune complex is detected in the test fluid. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950702373A 1992-12-07 1993-12-06 Hepatitis C Viral (HCV) Non-Struct-3 Peptides, Antibodies To It, and Methods for Detecting HCV (HCV) NON-STRUCTURAC-3 PEPTIDES DETECTION OF HCV KR950704353A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP92203802 1992-12-07
EP92203802.1 1992-12-07
PCT/EP1993/003422 WO1994013699A1 (en) 1992-12-07 1993-12-06 Hepatitis c virus (hcv) non-structural-3 peptides, antibodies thereto and methods for the detection of hcv

Publications (1)

Publication Number Publication Date
KR950704353A true KR950704353A (en) 1995-11-20

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019950702373A KR950704353A (en) 1992-12-07 1993-12-06 Hepatitis C Viral (HCV) Non-Struct-3 Peptides, Antibodies To It, and Methods for Detecting HCV (HCV) NON-STRUCTURAC-3 PEPTIDES DETECTION OF HCV

Country Status (8)

Country Link
EP (1) EP0672065A1 (en)
JP (1) JPH08505131A (en)
KR (1) KR950704353A (en)
AU (1) AU6653394A (en)
CA (1) CA2151128A1 (en)
FI (1) FI952778A (en)
WO (1) WO1994013699A1 (en)
ZA (1) ZA939169B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2294690B (en) * 1994-11-01 1998-10-28 United Biomedical Inc Peptides effective for diagnosis and detection of hepatitis C infection
EP1195381A1 (en) * 2000-09-28 2002-04-10 Immusystems GmbH Hepatitis c virus epitopes specific for cd4+ t-cell lymphocytes

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR8807310A (en) * 1987-11-18 1990-03-13 Chiron Corp POLINUCLEOTIDIO, POLINUCLEOTIDIO RECOMBINANT, RECOMBINANT VECTOR, CELL HOSTESS, EXPRESSION SYSTEM RECOMBINANT, CELL TRANSFORMED, polypeptide, HCV, DE polypeptide PREPARATION, polypeptide OF HCV polypeptide PURIFIED, polypeptide RECOMBINANT, DE FUSION polypeptide, MONOCLONAL ANTIBODY, PREPARATION PURIFIED ANTIBODY POLYCLONAL, PARTICLE, POLYNUCLEOTID PROBE, ANTIBODY KIT FOR HCV EPETOPE, METHOD FOR POLYPEPTIDY PRODUCTION, METHOD FOR DETECTING HCV NUCLEIC ACIDS, IMMUNOENSAIO TO DETECT HCV ANTIGENE, IMMUNOENSAIO DETECTORIGIO ANTIGENE OF HCV, CELL, CELL INFECTED WITH HCV, METHOD FOR THE PRODUCTION OF ANTIBODIES FOR HCV AND METHOD FOR ISOLATING CDNA DERIVED FROM THE GENOME FROM AN UNIDENTIFIED INFECTIOUS AGENT

Also Published As

Publication number Publication date
JPH08505131A (en) 1996-06-04
CA2151128A1 (en) 1994-06-23
EP0672065A1 (en) 1995-09-20
FI952778A (en) 1995-07-19
FI952778A0 (en) 1995-06-06
WO1994013699A1 (en) 1994-06-23
ZA939169B (en) 1994-08-08
AU6653394A (en) 1994-07-04

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