KR930000127A - Non-A Non-B Hepatitis Diagnosis Methods and Vaccines - Google Patents

Abstract

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Description

Non-A Non-B Hepatitis Diagnosis Methods and Vaccines

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1 is a schematic diagram of a method for constructing a complementary gene (cDNA) fragment encoding a novel non-A non-B hepatitis antigen protein according to the present invention.

2 is a schematic representation of the plasmid vector pTZ18U.

Figure 3 is a schematic diagram of cloning the novel gene fragment of Figure 1 into the plasmid vector pTZ18U of Figure 2.

Claims (20)

A non-A non-B type hepatocarcinoma virus sequencing comprising a functional equivalent containing part or all of the nucleotide sequence shown in FIG. 4, or a portion homologous thereto. The non-A non-B hepatitis virus nucleotide sequence according to claim 1, comprising a genomic sequence of the non-A non-B hepatitis virus or a nucleotide sequence derived from cDNA. A base sequence probe for detecting non-A non-B hepatitis-related DNA by hybridizing the non-A non-B hepatitis virus nucleotide sequence according to claim 1 or 2. How to use from. Recombinant vector containing the nucleotide sequence according to claim 1. A host cell transformed with the recombinant vector according to claim 4. A protein or polypeptide produced by the transformed cell of claim 5. A protein or polypeptide encoded by the nucleotide sequence according to claim 1. Hosts transformed with recombinant vectors containing non-A hepatitis B virus sequences to detect viral antibodies present in individuals infected with non-A hepatitis B viruses A method for detecting a non-A hepatitis virus antibody, characterized by detecting an antibody bound to an antigen by reacting a protein or polypeptide antigen produced from a cell with an antibody to be detected under appropriate conditions. The anti-win provided in claim 8 is in a suitable solid phase, and the human immunoglobulin antibody horn labeled with an enzyme or radioisotope is detected using an antigen used in the solid phase. How to. A non-A hepatitis vaccine comprising a protein or polypeptide according to claim 6. A non-A, non-B hepatitis virus complementary gene (cDNA) sequence comprising a functional equivalent containing part or all of the nucleotide sequence shown in the 19th or a part homologous thereto. 12. The non-A non-B hepatitis virus nucleotide sequence of claim 11, wherein the non-A non-B hepatitis virus sequence comprises a genomic sequence of the non-A non-B hepatitis virus or a sequence derived from cDNA. A base sequence probe for detecting non-A hepatitis B-related DNA by hybridization technology for the non-A non-B hepatitis virus nucleotide sequence according to claim 11 or 12. How to use from. A recombinant plasmid containing the nucleotide sequence according to claim 11. A transformant transformed by the recombinant vector according to claim 14. A protein or polypeptide produced by the transformed strain of claim 15. A protein or polypeptide encoded by the nucleotide sequence according to claim 11. 17. A method comprising detecting a viral antibody present in an individual infected with a non-A, non-B hepatitis virus using the protein or polypeptide antigen produced by claims 15 and 16. The method of claim 17, wherein the antigen to be used is in a suitable solid phase, and the antibody bound to the antigen immobilized on the solid phase is detected using an anti-human immunoglobulin antibody labeled with an enzyme or a radioisotope or an antigen used in the solid phase How to. A non-A hepatitis vaccine comprising a protein or polypeptide according to claim 16. ※ Note: The disclosure is based on the initial application.