KR950704354A - Peptides FROM THE C33 REGION OF HCV, ANTIBODIES THERETO AND METHODS FOR THE (C) Hepatitis Virus (HCV) DETECTION OF HCV - Google Patents

Peptides FROM THE C33 REGION OF HCV, ANTIBODIES THERETO AND METHODS FOR THE (C) Hepatitis Virus (HCV) DETECTION OF HCV

Info

Publication number
KR950704354A
KR950704354A KR1019950702374A KR19950702374A KR950704354A KR 950704354 A KR950704354 A KR 950704354A KR 1019950702374 A KR1019950702374 A KR 1019950702374A KR 19950702374 A KR19950702374 A KR 19950702374A KR 950704354 A KR950704354 A KR 950704354A
Authority
KR
South Korea
Prior art keywords
hcv
antibody
test fluid
peptide
acid sequence
Prior art date
Application number
KR1019950702374A
Other languages
Korean (ko)
Inventor
비난드 요한네스 안토니우스 하베츠
피에테르 야콥 보엔데르
Original Assignee
에프.쥐.엠, 헤르만스 페.카.브론스
악조 노벨 엔.브이.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 에프.쥐.엠, 헤르만스 페.카.브론스, 악조 노벨 엔.브이. filed Critical 에프.쥐.엠, 헤르만스 페.카.브론스
Publication of KR950704354A publication Critical patent/KR950704354A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C07K14/02—Hepadnaviridae, e.g. hepatitis B virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Communicable Diseases (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 HCV에 대한 항체에 면역화학적으로 반응하는 펩티드에 관한 것이다. 본 발명의 바람직한 펩티드는 NS-3프로테인의 HCV특이적 에피토프를 함유한다. 본 발명은 또한 HCV의 NS-3단백질에 특이적으로 반응하는 항체에 관한 것이다. HCV 또는 HCV 항체 검출 방법, NS-3 항원과 특이적으로 반응하는 항체의 검출하는 방법 또한 본 발명의 영역이다.The present invention relates to peptides that immunochemically respond to antibodies against HCV. Preferred peptides of the present invention contain HCV specific epitopes of NS-3 protein. The present invention also relates to antibodies that specifically react to the NS-3 protein of HCV. Methods of detecting HCV or HCV antibodies, and methods of detecting antibodies that specifically react with the NS-3 antigen, are also within the scope of the present invention.

Description

씨 (C)형 간염 바이러스(HCV) 씨 (C) 33 영역 유래의 펩티드, 이에 대한 항체 및 씨 (C)형 간염 바이러스를 검출하는 방법 (PEPTIDES FROM THE C33 REGION OF HCV, ANTIBODIES THERETO AND METHODS FOR THE DETECTION OF HCV)Hepatitis C Virus (HCV) Peptides from Antibody (C) 33 Regions, Antibodies Against It, and Methods for Detecting Hepatitis C Virus (PEPTIDES FROM THE C33 REGION OF HCV) DETECTION OF HCV

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 NS3단백질에 대한 단일 클론 항체(동일 출원인에 의한 공계류중인 출원 번호 제 EP 92.274.107.4호에 HCVHU. OT3로서 기재; 상기 출원의 내용은 본문에 참고 인용함)의 결합 특이성을 나타낸 그래프이다.1 is a graph showing the binding specificity of a monoclonal antibody against NS3 protein (described as HCVHU.OT3 in co-pending application number EP 92.274.107.4 by the same applicant; the contents of this application are incorporated herein by reference). to be.

제2도는 재조합 면역 블롯 측정의 그래프이다.2 is a graph of recombinant immune blot measurements.

Claims (15)

HCV-항체와 면역화학적으로 반응성인 서열 동정 번호 7로 도시된 아미노산 서열을 갖는 펩티드, 이의 단편 또는 이의 아날로그.Peptide, fragment or analog thereof having an amino acid sequence shown as SEQ ID NO: 7 immunochemically reactive with HCV-antibody. HCV-항체에 면역화학적으로 반응성인 서열 동정 번호 6, 8, 9, 10으로 도시된 서열 그룹에서 선택된 아미노산 서열을 갖는 펩티드 및 이의 조합물, 또는 이의 단편 또는 이의 아날로그.Peptides having a amino acid sequence selected from the group of sequences shown in SEQ ID NOs: 6, 8, 9, 10, and combinations thereof, or fragments thereof, that are immunochemically reactive to HCV-antibodies. 제1항 내지 제2항중 어느 한 항에 따른 펩티드를 함유하는 면역화학적 반응 시약.An immunochemical reaction reagent containing the peptide according to any one of claims 1 to 2. 제1항 내지 제2항중 어느 한 항에 따른 펩티드를 암호하는 핵산서열.A nucleic acid sequence encoding a peptide according to any one of claims 1 to 2. 제4항에 있어서, 서열 동정 번호 2로 도시된 DNA 서열 적어도 일부를 함유하는 핵산 서열.The nucleic acid sequence of claim 4 containing at least a portion of the DNA sequence shown as SEQ ID NO: 2. 제4항에 있어서, 서열 동정 번호 1, 3, 4, 5로 도시된 DNA 서열 적어도 일부를 함유하는 핵산 서열.The nucleic acid sequence of claim 4 containing at least a portion of the DNA sequence shown as SEQ ID NOs: 1, 3, 4, 5. 6. 제4항 내지 제6항 중 어느 한 항에 따른 핵산 서열을 함유하는 재조합 벡터.Recombinant vector containing a nucleic acid sequence according to any one of claims 4 to 6. 제7항에 따른 벡터로 형질 전환된 숙주 세포.A host cell transformed with the vector according to claim 7. 제1항 내지 제2항중 어느 한 항에 따른 펩티드에 대한 단일 클론항체.Monoclonal antibody against the peptide according to any one of claims 1 to 2. 제9항에 따른 항체를 분비 할 수 있는 무한 증식 세포주.An infinitely proliferating cell line capable of secreting the antibody according to claim 9. 제9항에 따른 단일 클론 항체를 분비할 수 있는 면역 진단 반응 시약.An immunodiagnostic reaction reagent capable of secreting the monoclonal antibody according to claim 9. 제1항 내지 제2항 중 어느 한 항에 따른 펩티드를 테스트 유체와 접촉시키고 테스트 유체중의 펩티드와 항체 사이에 형성된 면역 복합체의 존재를 면역 검출하는 것을 특징으로 하는, HCV에 대한 항체를 테스트 유체중에서 검출하는 방법.A test fluid for testing an antibody against HCV, characterized in that the peptide according to any one of claims 1 to 2 is contacted with a test fluid and immunodetection of the presence of an immune complex formed between the peptide and the antibody in the test fluid. How to detect in the middle. 시료를 제9항에 따른 단일 클론 항체와 접촉하고, 단일 클론 항체와 C형 간염 항원간에 형성된 면역 복합체를 검출하는 것을 특징으로 하는, C형 간염 바이러스를 시료중에서 검출하는 방법.A method for detecting hepatitis C virus in a sample, wherein the sample is contacted with the monoclonal antibody according to claim 9 and the immune complex formed between the monoclonal antibody and the hepatitis C antigen is detected. 제1항 또는 제2항에 따른 펩티드를 테스트 유체와 접촉하고, 펩티드와 항체간에 형성된 면역 복합체의 존재를 검출하는 것을 특징으로 하는 C형 간염 바이러스 NS-3단백질의 HCV특이적 에피토프에 대한 항체를 테스트 유체중에서 검출하는 방법.An antibody against HCV-specific epitope of hepatitis C virus NS-3 protein, characterized by contacting a peptide according to claim 1 or 2 with a test fluid and detecting the presence of an immune complex formed between the peptide and the antibody. Method of detection in test fluid. 적당한 지지체상에 피복된 제1항 또는 제2항에 따른 펩티드를 테스트 유체와 접촉한 뒤 제9항에 따른 항체와 접촉하고, 표지를 제공하고, 고체상에 결합된 어떠한 표지도 검출하는 것을 특징으로 하는, C형 간염 바이러스의 NS-3 단백질의 HCV 특이적 에프토프에 대한 항체를 테스트 유체중에서 검출하는 방법.A peptide according to claim 1 or 2 coated on a suitable support is contacted with a test fluid followed by contact with the antibody according to claim 9, providing a label, and detecting any label bound to a solid phase. A method of detecting, in a test fluid, an antibody against HCV-specific effope of NS-3 protein of hepatitis C virus. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950702374A 1992-12-07 1993-12-07 Peptides FROM THE C33 REGION OF HCV, ANTIBODIES THERETO AND METHODS FOR THE (C) Hepatitis Virus (HCV) DETECTION OF HCV KR950704354A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP92203802.1 1992-12-07
EP92203802 1992-12-07
EP93201854.2 1993-06-25
EP93201854 1993-06-25
PCT/EP1993/003478 WO1994013700A1 (en) 1992-12-07 1993-12-07 Peptides from the c33 region of hcv, antibodies thereto and methods for the detection of hcv

Publications (1)

Publication Number Publication Date
KR950704354A true KR950704354A (en) 1995-11-20

Family

ID=26131841

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019950702374A KR950704354A (en) 1992-12-07 1993-12-07 Peptides FROM THE C33 REGION OF HCV, ANTIBODIES THERETO AND METHODS FOR THE (C) Hepatitis Virus (HCV) DETECTION OF HCV

Country Status (7)

Country Link
EP (1) EP0672066A1 (en)
JP (1) JPH08504421A (en)
KR (1) KR950704354A (en)
AU (1) AU5809694A (en)
CA (1) CA2151126A1 (en)
FI (1) FI952779A (en)
WO (1) WO1994013700A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639594A (en) * 1990-02-16 1997-06-17 United Biomedical, Inc. Linear and branched peptides effective in diagnosing and detecting non-A, non-B hepatitis
FR2737209B1 (en) * 1995-07-25 1997-09-19 Bio Merieux PEPTIDE CAPABLE OF BEING RECOGNIZED BY ANTIBODIES RECOGNIZING THE C33 ANTIGEN OF HEPATITIS C VIRUS
EP1691198B1 (en) * 2003-10-28 2010-10-20 Advanced Life Science Institute, Inc. Method of detecting hepatitis c virus

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU220204B (en) * 1987-11-18 2001-11-28 Chiron Corp. Hcv polipeptides, hcv polinucleotides-containing such polinucleotides vectors and host cells-transformed with those diagnostics for detecting hcv infections, and immunoassay kit
AU7679491A (en) * 1990-04-04 1991-10-30 Protos, Inc. Hepatitis c virus protease inhibitors

Also Published As

Publication number Publication date
WO1994013700A1 (en) 1994-06-23
CA2151126A1 (en) 1994-06-23
JPH08504421A (en) 1996-05-14
AU5809694A (en) 1994-07-04
EP0672066A1 (en) 1995-09-20
FI952779A0 (en) 1995-06-06
FI952779A (en) 1995-07-31

Similar Documents

Publication Publication Date Title
EP0445423A2 (en) Hepatitis C assay
RU2007101725A (en) IDENTIFICATION OF VIRUS-INFECTED AND VIRUS-VACCINATED ORGANISMS
KR930004055B1 (en) Peptide and its use
US5229491A (en) Peptides immunochemically reactive with antibodies directed against hepatitis non-a, non-b virus
EP0696640A3 (en) Recombinant antigen from the NS3 region of Hepatitis C virus
AU2679492A (en) Hepatitis c assay
KR950704354A (en) Peptides FROM THE C33 REGION OF HCV, ANTIBODIES THERETO AND METHODS FOR THE (C) Hepatitis Virus (HCV) DETECTION OF HCV
DK0747481T3 (en) Epstein-Barr virus peptides and antibodies to these peptides
JPH06247997A (en) Hcv peptide antigen and method for measuring hcv
US20030108563A1 (en) Reagents for the simultaneous detection of HCV core antigens and antibodies
KR930004328A (en) Non-A, Non-B Type Peptides
KR950704353A (en) Hepatitis C Viral (HCV) Non-Struct-3 Peptides, Antibodies To It, and Methods for Detecting HCV (HCV) NON-STRUCTURAC-3 PEPTIDES DETECTION OF HCV
DK0607425T3 (en) Epstein-Barr virus peptides and antibodies to these peptides
KR950700423A (en) MONOCONAL ANTIBODIES AND ANTI-IDIOTYPIC ANTIBODIES TO HEPATITIS C VIRUS
KR960034420A (en) A multimeric antigen peptide consisting of at least two hepatitis C virus-related peptides
KR930007970A (en) Non-A, Non-Type Peptides
Perez et al. Antigenicity of chimeric and cyclic synthetic peptides based on nonstructural proteins of GBV‐C/HGV
JP4283527B2 (en) HCV core protein sequence
CA2052702A1 (en) Peptides immunochemically reactive with antibodies directed against hepatitis non-a, non-b virus
WO1993011158A2 (en) Non-a, non-b peptides
KR960014341A (en) Toxoplasma Gondi Antigen
JPH07502996A (en) Peptide showing immunochemical reactivity with antibodies against non-A, non-B hepatitis viruses
KR930700551A (en) Non-A, Non-B Sequences
JPH05307040A (en) Reagent for inspection of c-type hepatitis and inspecting method
Fields et al. Recombinant Proteins and Synthetic Peptides Containing Antigenic Epitopes of the Hepatitis C Virus Proteins and Development of a New Diagnostic Test Format

Legal Events

Date Code Title Description
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid