KR950701391A - T세포 항원 리셉터 유전자 발현의 검출방법 - Google Patents

T세포 항원 리셉터 유전자 발현의 검출방법

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Publication number
KR950701391A
KR950701391A KR1019940703755A KR19940703755A KR950701391A KR 950701391 A KR950701391 A KR 950701391A KR 1019940703755 A KR1019940703755 A KR 1019940703755A KR 19940703755 A KR19940703755 A KR 19940703755A KR 950701391 A KR950701391 A KR 950701391A
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KR
South Korea
Prior art keywords
antigen receptor
cell antigen
chain
gene
region
Prior art date
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KR1019940703755A
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English (en)
Inventor
가즈히코 야마모토
유타카 미즈시마
쿠즈키 니시오카
히로코 사코다
요코 이케다
Original Assignee
원본미기재
다이쇼 세이야쿠 가부시키가이샤
가부시키가이샤 일티티 겐큐쇼
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Application filed by 원본미기재, 다이쇼 세이야쿠 가부시키가이샤, 가부시키가이샤 일티티 겐큐쇼 filed Critical 원본미기재
Publication of KR950701391A publication Critical patent/KR950701391A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

피검시료중의 T세포에서 cDNA 중의 T세포 항원 리셉터 유전자를 PCR법에 의해 증폭하고, 이어서 증폭된 유전자를 싱글 스트렌드 콘포메이션 폴리모르피즘(SSCP)법에 의해 해석함으로서 피검시료에 직접하여 있는 T세포 클론에 특이적인 T세포 항원 리셉터 유전자의 존재를 검출할 수 있다.

Description

T세포 항원 리셉터 유전자 발현의 검출방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (7)

  1. T세포를 함유하는 피검시료로부터 T세포 유래의 cDNA를 함유하는 DNA 혼합물을 조제하고; 이 cDNA의 혼합물을 T세포 항원 리셉터의 β사슬을 코드하는 DNA 단편을 프라이머로서 사용하는 폴리메나제 체인 리액션(PCR)에 걸고, 이 cDNA의 혼합물중의 T세포 항원 리셉터의 β사슬을 코드하는 유전자를 증폭하고; 이어서 증폭된 유전자를 싱글 스트렌드 콘포메이션 폴리모르피즘(SSCP)법에 의한 해석에 걸고, T세포 항원 리셉터의 β사슬을 코두하는 유전자로 피검시료중에 직접하여 있는 T세포 클론에특이적인 유전자의 존재를 검출하는; 것으로 이루어진 T세포 항원 리셉터 유전자 발현의 검출방법.
  2. 제1항에 있어서, 피검시료가 면연응답이상이 의문되는 환자, 바이러스성 질환환자, 담암환자 또는 간염환자의 체액 또는 조직에서 얻어진 것인 검축방법.
  3. 제1항 또는 제2항에 있어서 PCR의 프라이머로서, T세포 항원 리셉터의 β사슬 V영역을 코드하는 DNA단편 및 β사슬 C영역을 코드하는 DNA 단편을 사용하는 검출방법.
  4. 제3항에 있어서, PCR의 프라이머로서 사용하는 T세포 항원 리셉터의 β의 V영역을 코드하는 DNA 단편이 Vβ1에서 Vβ20의 서브타입에서 선택된 영역을 코드하는 DNA 단편인 검출방법.
  5. 제1항 내지 제4항의 어느 한항에 있어서, SSCP법에 의한 해석을 글리세롤을 첨가한 가교도가 낮은 아크릴아미드 겔을 사용하여 수행하는 검출방법.
  6. 제1항 내지 제5항의 어느 한 항에 있어서, 피검시료중에 직접하여 있는 T세포 클론에 특이적인 유전자로서 T세포 항원 리셉터 β사슬 D영역 및/또는 β사슬 J영역을 코드하는 DNA 배열부분에 특이적인 배열을 갖는 T세포 클론과 그의 유전자의 존재를 검출하는 검출방법.
  7. 제1항 내지 제6항의 어느 한 항에 있어서, SSCP법에 의한 해석을 T세포 항원 리셉터의 β사슬 J영역을 코드하는 DNA 단편을 프로우브로서 사용하는 하이브리다이제이션법에 의해 수행하는 검출방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940703755A 1992-04-30 1993-04-30 T세포 항원 리셉터 유전자 발현의 검출방법 KR950701391A (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP111467/1992 1992-04-30
JP11146792 1992-04-30
JP205054/1992 1992-07-31
JP20505492 1992-07-31
PCT/JP1993/000577 WO1993022455A1 (en) 1992-04-30 1993-04-30 Method of detecting expression of t-cell antigen receptor gene

Publications (1)

Publication Number Publication Date
KR950701391A true KR950701391A (ko) 1995-03-23

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Family Applications (1)

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KR1019940703755A KR950701391A (ko) 1992-04-30 1993-04-30 T세포 항원 리셉터 유전자 발현의 검출방법

Country Status (5)

Country Link
EP (1) EP0653493A4 (ko)
KR (1) KR950701391A (ko)
AU (1) AU667922B2 (ko)
CA (1) CA2134460A1 (ko)
WO (1) WO1993022455A1 (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2134460A1 (en) * 1992-04-30 1993-11-11 Kazuhiko Yamamoto Method for detecting expression of t cell receptor genes
US6087096A (en) * 1995-11-13 2000-07-11 Dau; Peter C. Method of intrafamily fragment analysis of the T cell receptor α and β chain CDR3 regions
IL130074A0 (en) * 1996-11-22 2000-02-29 Shiloov Medical Technologies L A whole blood/mitogen assay for the early detection of a subject with cancer and kit
ES2331499B1 (es) * 2007-08-02 2010-10-14 Fundacion Publica Andaluza Para La Gestion De La Investigacion En Salud En Sevilla (Fund.Sevilla) Cebadores, procedimiento y conjunto de utiles para la determinacion d e la funcionalidad del timo humano.
PT2663864T (pt) * 2011-01-14 2019-06-21 Irepertoire Inc Método de avaliação de imunodiversidade e seu uso

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873190A (en) * 1984-06-13 1989-10-10 Massachusetts Institute Of Technology Heterodimeric T lymphocyte receptor
US4886743A (en) * 1985-04-24 1989-12-12 California Institute Of Technology Diagnostic reagents based on unique sequences within the variable region of the T cell receptor and uses thereof
WO1990004648A1 (en) * 1988-10-20 1990-05-03 Alexander Alan Morley Method for diagnosis of monoclonality in leukaemia and lymphoma
EP0527199B2 (en) * 1990-05-01 2000-08-30 The Board Of Trustees Of The Leland Stanford Junior University Compositions for use in a method for treating a human suffering from multiple sclerosis
CA2086015A1 (en) * 1990-06-20 1991-12-21 Jayne S. Danska Identification of cell subpopulations using modified pcr to amplify expression intermediates
FR2671356B1 (fr) * 1991-01-09 1993-04-30 Inst Nat Sante Rech Med Procede de description des repertoires d'anticorps (ab) et des recepteurs des cellules t (tcr) du systeme immunitaire d'un individu.
CA2134460A1 (en) * 1992-04-30 1993-11-11 Kazuhiko Yamamoto Method for detecting expression of t cell receptor genes

Also Published As

Publication number Publication date
WO1993022455A1 (en) 1993-11-11
AU4271693A (en) 1993-11-29
EP0653493A4 (en) 1996-03-27
CA2134460A1 (en) 1993-11-11
EP0653493A1 (en) 1995-05-17
AU667922B2 (en) 1996-04-18

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