KR950023710A - Improved high concentration cultivation method of lactic acid bacteria by mixing pH regulator - Google Patents

Improved high concentration cultivation method of lactic acid bacteria by mixing pH regulator Download PDF

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Publication number
KR950023710A
KR950023710A KR1019940001029A KR19940001029A KR950023710A KR 950023710 A KR950023710 A KR 950023710A KR 1019940001029 A KR1019940001029 A KR 1019940001029A KR 19940001029 A KR19940001029 A KR 19940001029A KR 950023710 A KR950023710 A KR 950023710A
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KR
South Korea
Prior art keywords
lactic acid
acid bacteria
high concentration
mixing
culture medium
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KR1019940001029A
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Korean (ko)
Inventor
서인영
신명수
이정준
나석환
백영진
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이은선
한국야쿠르프유업 주식회사
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Priority to KR1019940001029A priority Critical patent/KR950023710A/en
Publication of KR950023710A publication Critical patent/KR950023710A/en

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Abstract

본 발명은 우레아제를 생산할 수 있는 젖산균 및 우레아제를 생산할 수 없는 젖산균의 배양시 배양배지에 특성이 다른 pH 조절물질을 혼용하여 적용시킴으로써 이들 젖산균을 고농도로 대량 배양할 수 있는 개량된 방법에 관한 것이다.The present invention relates to an improved method for mass culturing these lactic acid bacteria at high concentration by applying a mixture of pH regulators having different characteristics to the culture medium when culturing the lactic acid bacteria capable of producing urease and lactic acid bacteria that cannot produce urease.

본 발명에 따른 방법에서 사용될 수 있는 배양배지는 천연 배지 및 합성배지를 포함한다.Culture media that can be used in the method according to the invention include natural media and synthetic media.

Description

pH 조절물질을 혼용함에 의한 젖산균의 개량된 고농도 배양방법Improved high concentration cultivation method of lactic acid bacteria by mixing pH regulator

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (4)

0.8-1.0부피%의 우레아를 첨가시킨 배양 배지를 제조하는 단계 및 28.5부피%의 암모니아수를 외부 중화 물질로 사용하여 배양액의 pH를 5.5-7.0로 조절함으로써 우레아제 효소를 생산할 수 있는 젖산균을 고농도로 배양시키는 단계를 포함하는 것을 특징으로 하는 pH 조절물질을 혼용함에 의한 젖산균의 개량된 고농도 대량 배양 방법.The step of preparing a culture medium containing 0.8-1.0% by volume of urea and 28.5% by volume of ammonia water as an external neutralizing material to adjust the pH of the culture medium to 5.5-7.0 to cultivate a high concentration of lactic acid bacteria that can produce urease enzyme An improved high concentration mass culture method of lactic acid bacteria by mixing a pH adjusting material, characterized in that it comprises a step of. 제1항에 있어서, 상기 배양 배지는 천연 배지 또는 함성배지인 것을 특징으로 하는 배양방법.The method of claim 1, wherein the culture medium is a culture method, characterized in that the natural medium or containing medium. 1.5-2.0부피%의 베타 글리세로 인산염을 첨가시킨 배양배지를 제조하는 단계 및 28.5부피%의 암모니아수를 외부 중화 물질로 사용하여 배양액의 pH를 5.5-7.0으로 조절함으로써 우레아제 효소를 생산할 수 없는 젖산균을 고농도로 배양시키는 단계를 포함하는 것을 특징으로 하는 pH 조절물질을 혼용함에 의한 젖산균의 개량된 고농도 대량 배양방법.Preparing a culture medium containing 1.5-2.0% by volume of beta glycerol and 28.5% by volume of ammonia water as an external neutralizer to adjust the pH of the culture medium to 5.5-7.0 to prevent lactic acid bacteria that cannot produce urease enzyme. An improved high concentration bulk culture method of lactic acid bacteria by mixing a pH adjusting material, characterized in that it comprises a step of culturing at a high concentration. 제3항에 있어서, 상기 배양 배지는 천연 배지 또는 합성 배지인 것을 특징으로 하는 배양방법.The method of claim 3, wherein the culture medium is a natural medium or a synthetic medium. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940001029A 1994-01-20 1994-01-20 Improved high concentration cultivation method of lactic acid bacteria by mixing pH regulator KR950023710A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940001029A KR950023710A (en) 1994-01-20 1994-01-20 Improved high concentration cultivation method of lactic acid bacteria by mixing pH regulator

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Application Number Priority Date Filing Date Title
KR1019940001029A KR950023710A (en) 1994-01-20 1994-01-20 Improved high concentration cultivation method of lactic acid bacteria by mixing pH regulator

Publications (1)

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KR950023710A true KR950023710A (en) 1995-08-18

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