KR940014778A - Method for preparing immobilized cyclodextrin glucano transferase - Google Patents

Method for preparing immobilized cyclodextrin glucano transferase Download PDF

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Publication number
KR940014778A
KR940014778A KR1019920024879A KR920024879A KR940014778A KR 940014778 A KR940014778 A KR 940014778A KR 1019920024879 A KR1019920024879 A KR 1019920024879A KR 920024879 A KR920024879 A KR 920024879A KR 940014778 A KR940014778 A KR 940014778A
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KR
South Korea
Prior art keywords
resin
enzyme
transferase
anion exchange
immobilized
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KR1019920024879A
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Korean (ko)
Inventor
인만진
김동청
최경호
김민홍
노홍식
Original Assignee
유영학
주식회사 미원
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Priority to KR1019920024879A priority Critical patent/KR940014778A/en
Publication of KR940014778A publication Critical patent/KR940014778A/en

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Abstract

본 발명에 따르면 다공성 강염기 음이온 교환수지, 또는 다공성 약염기 음이온 교환수지로서 그 표면적이 1.0㎡/g이상이고, 전 세공용적이 0.1ml/g 이상이고,공반경의 번위가 20~2,000А인 수지에 사이클로덱스 트린글루카노 트랜스퍼라제(EC.3.2.1.19)함유액을 접촉시켜 이 효소를 수지에 흡착시키는 것을 특징으로 하는 고정화 사이클로덱스트린글루카노 트랜스퍼라제의 제조방법이 제공된다. 본 발명의 방법에 따라 제공되는 고정화 효소는 안정하고 효소 활성이 높으며 제조 비용이 저렴하기 때문에 산업적으로 유용하게 사용될수 있다.According to the present invention, a porous strong base anion exchange resin, or a porous weak base anion exchange resin, has a surface area of 1.0 m 2 / g or more, a total pore volume of 0.1 ml / g or more, and a pore radius of 20 to 2,000 A. Provided is a method for preparing an immobilized cyclodextrin glucano transferase, characterized in that said enzyme is adsorbed onto a resin by contacting a cyclodextrin glucano transferase (EC.3.2.1.19) containing solution. The immobilized enzyme provided according to the method of the present invention can be used industrially because it is stable, high in enzyme activity and low in manufacturing cost.

Description

고정화 사이클로덱스트린글루카노 트랜스퍼라제의 제조방법.Method for preparing immobilized cyclodextrin glucano transferase.

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 각 pH구간에서의 고정화 효소의 활성을 나타내고,1 shows the activity of the immobilized enzyme in each pH section,

제2도는 효소액의 이온농도에 따른 고정화 효소의 활성을 나타내며,2 shows the activity of the immobilized enzyme according to the ion concentration of the enzyme solution,

제3도는 수지 g당 단백질 투입량에 따른 고정화 효소의 활성을 나타낸다.3 shows the activity of the immobilized enzyme according to the protein input per gram of resin.

Claims (4)

다공성 강염기 음이온 교환수지, 또는 다공성 약염기 음이온 교환수지로서 그 표면적이 1.0㎡/g이상이고, 전 세공용적이 0.1ml/g 이상이고, 공반경의 범위가 20~2000A인 수지에 사이클로덱스 트린글루카노 트랜스퍼라제(EC.3.2.1.19)함유액을 접촉시켜 이 효소를 수지에 흡착시키는 것을 특징으로 하는 고정화 사이클로덱스트린글루카노 트랜스퍼라제의 제조방법.Porous strong base anion exchange resin, or porous weak base anion exchange resin, cyclodextrin glucano in resin having a surface area of 1.0 m 2 / g or more, total pore volume of 0.1 ml / g or more and a pore radius of 20 to 2000 A. A method for producing an immobilized cyclodextrin glucano transferase, characterized by adsorbing the enzyme to a resin by contacting a transferase (EC.3.2.1.19) -containing liquid. 제1항에 있어서, 흡착 pH를 6~10으로 하는 방법.The method according to claim 1, wherein the adsorption pH is set to 6-10. 제1항에 있어서, 효소액중의 이온농도를 0.02~0.05M로 하는 방법.The method according to claim 1, wherein the ion concentration in the enzyme solution is 0.02 to 0.05M. 제1항에 있어서, 수지 1g에 대하여 20~30mg 당량에 해당하는 효소를 투입하여 고정화하는 방법.The method according to claim 1, wherein an enzyme corresponding to 20 to 30 mg equivalent is added to 1 g of the resin to be immobilized. ※참고사항:최초출원 내용에 의하여 공개하는 것임.※ Note: This is to be disclosed based on the first application.
KR1019920024879A 1992-12-21 1992-12-21 Method for preparing immobilized cyclodextrin glucano transferase KR940014778A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019920024879A KR940014778A (en) 1992-12-21 1992-12-21 Method for preparing immobilized cyclodextrin glucano transferase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019920024879A KR940014778A (en) 1992-12-21 1992-12-21 Method for preparing immobilized cyclodextrin glucano transferase

Publications (1)

Publication Number Publication Date
KR940014778A true KR940014778A (en) 1994-07-19

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KR1019920024879A KR940014778A (en) 1992-12-21 1992-12-21 Method for preparing immobilized cyclodextrin glucano transferase

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