KR940019857A - Purification Method of Eurokinase - Google Patents

Purification Method of Eurokinase Download PDF

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Publication number
KR940019857A
KR940019857A KR1019930001356A KR930001356A KR940019857A KR 940019857 A KR940019857 A KR 940019857A KR 1019930001356 A KR1019930001356 A KR 1019930001356A KR 930001356 A KR930001356 A KR 930001356A KR 940019857 A KR940019857 A KR 940019857A
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KR
South Korea
Prior art keywords
urokinase
sepharose
purifying
eurokinase
solution
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KR1019930001356A
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Korean (ko)
Inventor
주한승
곽원재
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연만희
주식회사 유한양행
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Priority to KR1019930001356A priority Critical patent/KR940019857A/en
Publication of KR940019857A publication Critical patent/KR940019857A/en

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Abstract

본 발명은 유로키나제의 정제방법에 관한 것으로는 더 상세하게는 pH6-8의 완충매질에서 친화성 메트릭스에 유로키나제를 시킨 후, pH8이상의 염기성 완충용액으로 유로키나제를 용출시킴으로써 조유로키나제액으로부터 고순도 및 고활성의 유로키나제를 졍제하는 방법에 관한 것이다.The present invention relates to a method for purifying urokinase, and more particularly, by subjecting urokinase to an affinity matrix in a buffer medium of pH 6-8, and eluting urokinase with a basic buffer solution of pH 8 or higher, high purity and high activity from crude urokinase solution. It is about a method for removing eurokinase.

Description

유로키나제의 정제 방법Purification Method of Eurokinase

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (5)

조유로키나제액을 친화성 다공질 매트릭스 컬럼을 통하여 흡착시킨 후, 이를 용출시킨 유로키나제를 정제하는 방법에 있어서, 친화성 매트릭스컬럼에 pH6-8의 완충용액으로 조유로키나제를 흡착시키고, pH8이상의 알카리용액으로 유로키나제를 용출시키는 것을 특징으로 하는 유로키나제 정제방법.In the method of adsorbing a crude eurokinase solution through an affinity porous matrix column and purifying the eluted urokinase, the crude eurokinase is adsorbed to the affinity matrix column with a buffer solution of pH 6-8, and the urokinase with an alkaline solution of pH 8 or higher. Eurokinase purification method characterized by eluting. 제1항에 있어서, 용출완충용액의 총염농도가 0.05-2몰인 것을 특징으로 하는 유로키나제 정제 방법.The method of purifying urokinase according to claim 1, wherein the total salt concentration of the elution buffer solution is 0.05-2 mol. 제1항에 있어서, 용출액이 인산염완충액, 트리스완충액, 0.01-5% 암모니아수 중에서 선택된 것임을 특징으로 하는 유로키나제 정제 방법.The method of purifying urokinase according to claim 1, wherein the eluate is selected from phosphate buffer, tris buffer and 0.01-5% ammonia water. 제1항에 있어서, 친화성 메트릭스 컬럼의 다공질 매트릭스가 P-아미노벤미딘-세파로스, ε-아미노카프로산-세파로스, 유로키나제 항체-세파로스, 아그마틴-ε-카프로산-세파로스, 아프로티닌-세파로스, 라이신-아가로스, 또는 아르기닌-아가로스중에서 선택된 것임을 특징으로 하는 유로키나제 정제 방법.The method of claim 1, wherein the porous matrix of the affinity matrix column is P-aminobenmidine-sepharose, ε-aminocaproic acid-sepharose, urokinase antibody-sepharose, agmatine-ε-caproic acid-sepharose, apro A method for purifying urokinase, characterized in that it is selected from tinin-sepharose, lysine-agarose, or arginine-agarose. 제4항에 있어서, 친화성 메트릭스 컬럼의 다공질매트릭스가 P-아미노벤미딘-세라로스 또는 유로키나제항체-세파로스인 것을 특징으로 하는 유로키나제 정제 방법.5. The method of purifying urokinase according to claim 4, wherein the porous matrix of the affinity matrix column is P-aminobenmidine-cerarose or urokinase antibody-sepharose. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930001356A 1993-02-02 1993-02-02 Purification Method of Eurokinase KR940019857A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019930001356A KR940019857A (en) 1993-02-02 1993-02-02 Purification Method of Eurokinase

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Application Number Priority Date Filing Date Title
KR1019930001356A KR940019857A (en) 1993-02-02 1993-02-02 Purification Method of Eurokinase

Publications (1)

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KR940019857A true KR940019857A (en) 1994-09-15

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KR1019930001356A KR940019857A (en) 1993-02-02 1993-02-02 Purification Method of Eurokinase

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