KR930700646A - σ-시클로텍스트린 글리코실이전효소(σ-cyclodextrin glycosyl trans ferase) - Google Patents

σ-시클로텍스트린 글리코실이전효소(σ-cyclodextrin glycosyl trans ferase)

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KR930700646A
KR930700646A KR1019920702261A KR920702261A KR930700646A KR 930700646 A KR930700646 A KR 930700646A KR 1019920702261 A KR1019920702261 A KR 1019920702261A KR 920702261 A KR920702261 A KR 920702261A KR 930700646 A KR930700646 A KR 930700646A
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amino acid
region
gene
cyclodextrin
encoding
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KR1019920702261A
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KR960013463B1 (ko
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쉬미드 게르하르트
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프랑크 뮬러
콘소티움 퓌어 에렉트로헤미쉐 인더스트리 게엠베하
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Steroid Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

내용 없음.

Description

σ-시클로텍스트린 글리코실전이효소(σ-cyclodextrin glycosyl trans ferase)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 친알카리성 박테리움바실러스 290-3에서 얻어진 -CGTase에 의한 β- 및 γ-시클로덱스트린 생산 속도 그라프,
제5도는 발현 플라스미드 PCM750,
제7도는 폴리스미드 PCM720

Claims (14)

  1. 바실러스(bacillus) 290-3(DSM 5850)에서 얻어진 r-시클로덱스트린 그릴코실전이효소.
  2. 자연미생물기원(origin)에서 가급적 다량으로 취득할 수 있음을 특징으로 하는 제1항에 r-시클로덱스트린 글리코실전이효소의 아미노-종결영역내의 변경유도체.
  3. 제1항에 의한 r-시클로덱스트린 글리코실전이효소의 유전자, 즉 바실러스 1-1에서 얻어진 r-CGTase의 대응아미노산을 엔코팅하는 DNA영역에 의해 5′영역에서 치환되는 유전자에 의해 엔코팅(encoding)함을 특징으로 하는 r-시클로덱스트린 글리코실전이효소.
  4. 제1항 내지 제3항중 한항에 의해 얻어질 수 있는 시클로덱스트린 글리코실전이효소를 엔코팅(encoding)하는 발현 플라스미드(expression plasmds).
  5. (a) r-CGTase의 분비용으로 친알칼리성 전분분해 세균을 선별시켜 (b) 이들 세균을 분류학적으로 특성화하고, (c) 그 세균에서 얻어진 r-CGTase를 정제하여 생화학적으로 특성화하며, (d) r-CGTase의 유전자를 클로닝(cloning) 및 시 싱(sequencing)하고, (e) 이 유전자를 충분히 성장한 단백질의 5′영역을 엔코팅한 영역에서 변경시키며, (f) 이와같이 변경한 유전자를 조절할 수 있는 프로모터로 조절하고 세균에 도입시켜 그 유전자를 그 세균으로 증식시켜 그 유전자생성물이 분비될 수 있도록 하며, (g) 유도 및 발현 후에 배지상증액에서 효소를 취득함을 특징으로하는 r-시클로덱스트린 글리코실전이효소의 취득방법.
  6. 제5항에 있어서, 상기 공정 (e)에서 5′영역을 엔코팅하는 영역은 또다른 r-CGTase의 대응영역을 엔코딩하는 영역에 의해 대치시킴을 특징으로하는 상기 방법.
  7. 제6항에 있어서, 사용한 또다른 r-CGTase의 대응영역을 엔코딩하는 그 영역은 바실러스 1-1의 대응영역을 엔코딩하는 영역임을 특징으로 하는 상기 방법.
  8. r-시클로덱스트린 글리코실전이효소의 조절할 수 있는 발현이 가능하도록 신호펩티드를 사용하여 r-시클로덱스트린 글리코실전이효소를 코딩하는 유전자가 유전자 공학적적인 변경을 하여, 그 변경유전자를 분비 돌연변이체(secretor mutants)로 발현함을 특징으로 하는 r-시클로덱스트린 글리코실전이효소의 제조방법.
  9. 제8항에 있어서, 사용되는 r-시클로덱스트린 글리코실전이효소를 코팅하는 유전자가 제1항에 의한 r-시클로덱스트린 글리코실전이효소의 유전자임을 특징으로 하는 상기방법.
  10. 제2도에 나타낸 아미노산 시퀀스(sequence)를 엠코딩하는 DNA.
  11. 제2도의 아미노산75에서 시작하는 아미노산 시 스를 포함하는 r-시클로덱스트린 글리코실전이효소를 엔코딩하는 DNA.
  12. 아미노산 198에서 아미노산214이내 및/또는 아미노산 238에서 아미노산 247이내 및/또는 아미노산 267에서 아미노산 275이내 및/또는 아미노산 336에서 아미노산 343이내를 포함하는 제2도의 아미노산 영역을 포함하는 r-시클로덱스트린 글리코실전이효소를 엔코딩하는 DNA.
  13. 1항 내지 3항중 하나이상의 항에 의한 r-CGTase를 사용함을 특징으로하는 r-시클로덱스트린의 제조방법.
  14. 세균균주 바실러스 290-3 (DSM 5850).
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019920702261A 1990-03-27 1991-03-22 α-CG타아제 KR960013463B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE90-P4009822.2 1990-03-27
DE4009822A DE4009822A1 (de) 1990-03-27 1990-03-27 (gamma)-cgtase
PCT/EP1991/000560 WO1991014770A2 (de) 1990-03-27 1991-03-22 η-CGTase

Publications (2)

Publication Number Publication Date
KR930700646A true KR930700646A (ko) 1993-03-15
KR960013463B1 KR960013463B1 (ko) 1996-10-05

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US (1) US5409824A (ko)
EP (1) EP0521948B1 (ko)
JP (1) JPH0630575B2 (ko)
KR (1) KR960013463B1 (ko)
AT (1) ATE123808T1 (ko)
CA (1) CA2078992A1 (ko)
DE (2) DE4009822A1 (ko)
ES (1) ES2073743T3 (ko)
FI (1) FI924294A (ko)
HU (1) HUT63190A (ko)
TW (1) TW234146B (ko)
WO (1) WO1991014770A2 (ko)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW383336B (en) * 1993-06-24 2000-03-01 Consortium Elektrochem Ind Cyclodextrin glycosyl transferases for the preparation of gama-cyclodextrin
JP3429569B2 (ja) * 1994-08-30 2003-07-22 財団法人野田産業科学研究所 環状イソマルトオリゴ糖合成酵素遺伝子を含有するｄｎａ、組み換え体ｄｎａ、及び環状イソマルトオリゴ糖合成酵素の製造法
AR001678A1 (es) * 1995-04-21 1997-11-26 Novo Nordisk As Variantes de glucanotransferasa de ciclomaltodextrina construccion de adn vector de expresion recombinante celula huesped método para producir variantes usos de dichas variantes método para modificar la adhesion y/o especialmente de la enzima CGTasa precursora
DE19615336A1 (de) * 1996-04-18 1997-10-23 Consortium Elektrochem Ind Cyclodextringlycosyltransferasen zur Produktion von gamma-Cyclodextrin
US6472192B1 (en) 1996-04-18 2002-10-29 Consortium für elektrochemische Industrie GmbH Cyclodextrin glycosyl transferases for producing γ-cyclodextrin
JP3934851B2 (ja) * 2000-05-23 2007-06-20 日本食品化工株式会社 新規シクロテ゛キストリン・ク゛ルカノトランスフェラーセ゛、その製造方法及びこの酵素を用いるシクロテ゛キストリンの製造方法
US7700338B2 (en) 2000-07-19 2010-04-20 Novozymes A/S CGTase and DNA sequence encoding same
DK1337648T3 (da) * 2000-11-28 2008-01-07 Henkel Kgaa Ny cyclodextrin-glucanotransferase (CGTase) fra Bacillus agaradherens (DSM 9948) samt vaske- og rengöringsmidler med denne nye cyclodextrin-glucanotransferase
US6960461B2 (en) 2001-07-11 2005-11-01 Nihon Shokuhin Kako Co., Ltd. Gene coding for cyclodextrin glucanotransferase chiefly producing γ-cyclodextrin and use thereof
JP4989922B2 (ja) * 2006-06-08 2012-08-01 日本食品化工株式会社 変異体及びこれをコードする遺伝子

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3716181A1 (de) * 1987-05-14 1988-12-08 Consortium Elektrochem Ind Verfahren zur herstellung von cyclooctaamylose

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DE59105733D1 (de) 1995-07-20
EP0521948A1 (de) 1993-01-13
EP0521948B1 (de) 1995-06-14
CA2078992A1 (en) 1991-09-28
ES2073743T3 (es) 1995-08-16
TW234146B (ko) 1994-11-11
HUT63190A (en) 1993-07-28
JPH0630575B2 (ja) 1994-04-27
WO1991014770A3 (de) 1992-01-23
KR960013463B1 (ko) 1996-10-05
US5409824A (en) 1995-04-25
FI924294A0 (fi) 1992-09-25
ATE123808T1 (de) 1995-06-15
FI924294A (fi) 1992-09-25
DE4009822A1 (de) 1991-10-02
JPH05501059A (ja) 1993-03-04
WO1991014770A2 (de) 1991-10-03

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