KR930016539A - 재조합 종양괴사인자(TNF-α)의 개선된 정제방법 - Google Patents

재조합 종양괴사인자(TNF-α)의 개선된 정제방법 Download PDF

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KR930016539A
KR930016539A KR1019920000993A KR920000993A KR930016539A KR 930016539 A KR930016539 A KR 930016539A KR 1019920000993 A KR1019920000993 A KR 1019920000993A KR 920000993 A KR920000993 A KR 920000993A KR 930016539 A KR930016539 A KR 930016539A
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Prior art keywords
necrosis factor
tumor necrosis
tnf
recombinant tumor
recombinant
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KR1019920000993A
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English (en)
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KR950004015B1 (ko
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배태옥
박순재
윤상주
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최근선
주식회사 럭 키
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Priority to KR1019920000993A priority Critical patent/KR950004015B1/ko
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Publication of KR950004015B1 publication Critical patent/KR950004015B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/525—Tumour necrosis factor [TNF]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract

본 발명은 효모로부터 재조합 인체종양괴사인자(TNF-α)를 정제하는데 있어서, 부분적으로 정제된 조 종양괴사인자를 셀루파인 설페이트(cellufine-sulfate)를 담체로 사용한 친화성 크로마토그래피를 통하여 고순도로 정제하는 방법에 관한 것이다.

Description

재조합 종양괴사인자(TNF-α)의 개선된 정제방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명에 따른 재조합 인체 종양괴사자인자의 정제과정에 있어서 음이온 교한수지를 이용하여 분리한 크로마토 그래피의 패턴이다. 제2도는 본 발명에 따른 정제과정에 있어서 셀루파인 셀페이트를 이용하여 분리한 크로마토그래피의 패턴이다. 제3도는 본 발명에 따른 정제과정에 있어서 S-300를 이용하여 분리한 크로마토그래피의 패턴이다.

Claims (3)

  1. 조 종양 괴사인자로 부터 셀루파인 설페이트(cellufine sulfate) 친화성 크로마토그래피를 이용하여 재조합 종양괴사인자(TNF-α)를 정제하는 방법.
  2. 제1항에 있어서, 조 종양 괴사인자는 가)세포 분쇄액을 암모니움 설페이트 분별 침전시키는 과정, 나)페닐-세파로즈 크로마토 그래피, 다)음이온 교환 수지 크로마토그래피 등의 단계를 포함하는 정제과정을 통하여 수득된 것임을 특징으로 하는 방법.
  3. 제1항에 있어서, 재조합 종양괴사인자(TNF-α)는 재조합 인체, 원숭이 또는 쥐의 종양괴사인자인 것을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019920000993A 1992-01-24 1992-01-24 재조합 종양괴사인자(TNF-α)의 개선된 정제방법 KR950004015B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019920000993A KR950004015B1 (ko) 1992-01-24 1992-01-24 재조합 종양괴사인자(TNF-α)의 개선된 정제방법

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019920000993A KR950004015B1 (ko) 1992-01-24 1992-01-24 재조합 종양괴사인자(TNF-α)의 개선된 정제방법

Publications (2)

Publication Number Publication Date
KR930016539A true KR930016539A (ko) 1993-08-26
KR950004015B1 KR950004015B1 (ko) 1995-04-22

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KR1019920000993A KR950004015B1 (ko) 1992-01-24 1992-01-24 재조합 종양괴사인자(TNF-α)의 개선된 정제방법

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KR950004015B1 (ko) 1995-04-22

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