KR930013138A - Purification of Vancomycin Using Ion Exchange Resin and Alumina Resin - Google Patents

Purification of Vancomycin Using Ion Exchange Resin and Alumina Resin Download PDF

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KR930013138A
KR930013138A KR1019910024438A KR910024438A KR930013138A KR 930013138 A KR930013138 A KR 930013138A KR 1019910024438 A KR1019910024438 A KR 1019910024438A KR 910024438 A KR910024438 A KR 910024438A KR 930013138 A KR930013138 A KR 930013138A
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South Korea
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exchange resin
cation exchange
resin
vancomycin
anion exchange
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KR1019910024438A
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Korean (ko)
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KR950009201B1 (en
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유원일
이광섭
이시철
고중환
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김정순
제일제당 주식회사
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
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  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

본 발명은 Glycopeptide 항생제의 일종의 반코마이신의 정제에 관한 것이다. 즉, 토양으로 부터 분리한 Norcardia Orientalis(과거에는 Streptomyces Orientalis로 명명)를 배양하여 균첼르 분리시킨후, 여액을 양이온 및 음이온 교환수지, 다공성 타입의 양이온 교환수지를 연속 통액한 후 암모니아로 용출하고, 알루미나 및 약산성 양이온 교환수지를 사용하여 불순물이 제거된 높은 순도의 염산염 형태의 반코마이신을 분리, 회수함을 특징으로 하는 반코마이신의 정제 방법에 관한 것이다.The present invention relates to the purification of vancomycin, a kind of Glycopeptide antibiotic. That is, the culture of Norcardia Orientalis (formerly named Streptomyces Orientalis) isolated from the soil was inoculated with Bacillus, and the filtrate was continuously passed through cation and anion exchange resin, porous type cation exchange resin, and eluted with ammonia. The present invention relates to a method for purifying vancomycin, characterized by separating and recovering vancomycin in the form of high purity hydrochloride in which impurities are removed using alumina and a weakly acidic cation exchange resin.

Description

이온교환수지 및 알루미나 수지를 이용한 반코아미신의 정제Purification of Vancoamicin Using Ion Exchange Resin and Alumina Resin

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 발효여액, PK208 용리액, 알루미나 통과액 및 CM-Toyopearl 650의 용리액의 고압 액체크로마토그라피 분석결과이고,Figure 1 shows the results of high pressure liquid chromatography analysis of the fermentation filtrate, PK208 eluent, alumina pass-through and CM-Toyopearl 650 eluent.

제2도는 SK1B, A561(PA408)및 PK208 수지로부터의 반코마이신의 용리 그래프이며,2 is an elution graph of vancomycin from SK1B, A561 (PA408) and PK208 resins,

제3도는 CM-Toyopearl 650의 흡착 및 용리 그래프이고,3 is a graph of adsorption and elution of CM-Toyopearl 650,

제4도는 본 발명에 의해 제조된 최종 제품과 미국약전 표준품과의 고압액체 크로마토그라피 분석결과이다.4 is a high pressure liquid chromatography analysis of the final product prepared by the present invention and the US Pharmacopoeia standard.

Claims (4)

반코마이신을 함유하는 발효배양액에서 발효여액을 무기산으로 pH2.0-3.0으로 조절한 다음 강산성 양이온 교환수지 및 염기성 음이온 교환수지, 다공성 타입의 강산성 양이온 교환수지를 단계별로 단독 사용하거나 또는 연결하여 사용하여 다단계로 분리, 정제하고 pH11-13의 암모니아수로 용출하여 활성알루미나로 탈색해서 역삼투막으로 농축한뒤 약산성 양이온 교환수지로 흡착하고, 염화암모늄 완충액으로 용출한 뒤 에탄올로 정석하여 회수, 건조함을 특징으로 하는 반코마이신의 정제방법.In the fermentation broth containing vancomycin, the fermentation filtrate is adjusted to pH2.0-3.0 with inorganic acid, followed by the use of strong acidic cation exchange resin, basic anion exchange resin, and porous type strong acid cation exchange resin alone or in combination. Separated and purified by elution with ammonia water at pH 11-13, decolorized with activated alumina, concentrated with reverse osmosis membrane, adsorbed with weakly acidic cation exchange resin, eluted with ammonium chloride buffer, crystallized with ethanol and recovered and dried. Method for Purifying Vancomycin. 제1항에 있어서, 양이온 교환수지들의 경우 강산성 양이온 교환수지로서 스티렌계 젤형인 SK1B 또는 듀오리트 C-20(Duolite C-20)을 사용하고 다공성 타입의 강산성 양이온 교환수지로서 PK208, PK212를 사용하며 약산성 양이온 교환수지로서는 CM-Toyopearl 650DLSK 또는 CM-세파로스 FF수지를 사용함을 특징으로 하는 방법.The method of claim 1, wherein the cation exchange resins use styrenic gel type SK1B or Duolite C-20 as strong acid cation exchange resin and PK208, PK212 as strong acid cation exchange resin of porous type. CM-Toyopearl 650DLSK or CM-Sepharose FF resin is used as the weakly acidic cation exchange resin. 제1항에 있어서, 음이온 교환수지들의 경우 강염기성 음이온 교환수지로서 PA408과 같은 다공성 타입의 스티렌계 수지를 사용하거나 또는 약염기성 음이온 교환수지로서 A561과 같은 페놀릭계 수지를 사용함을 특징으로 하는 방법.The method according to claim 1, wherein the anion exchange resins use a porous type of styrene resin such as PA408 as the strong base anion exchange resin, or a phenolic resin such as A561 as the weak base anion exchange resin. 제1항에 있어서, 역삼투막은 폴리아미드, 폴리스티렌, 폴리설폰계의 재질로 하고 온도는 10-30℃, 압력은 10-40㎏/㎠로 하여 반코마이신의 농도를 50-200g/L까지 농축하는 것을 특징으로 하는 방법.The method of claim 1, wherein the reverse osmosis membrane is made of a polyamide, polystyrene, polysulfone-based material, the temperature is 10-30 ° C., the pressure is 10-40 kg / cm 2, and the concentration of vancomycin is increased to 50-200 g / L. How to feature. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019910024438A 1991-12-26 1991-12-26 Purification of vancomycin by using ion exchange resin and alumina resin KR950009201B1 (en)

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KR1019910024438A KR950009201B1 (en) 1991-12-26 1991-12-26 Purification of vancomycin by using ion exchange resin and alumina resin

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KR1019910024438A KR950009201B1 (en) 1991-12-26 1991-12-26 Purification of vancomycin by using ion exchange resin and alumina resin

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KR950009201B1 KR950009201B1 (en) 1995-08-16

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100385152B1 (en) * 2001-03-14 2003-05-23 씨제이 주식회사 Process for the purification of capreomycin
KR100419707B1 (en) * 1995-12-29 2004-06-12 씨제이 주식회사 Purification method of vancomycin using prep-hplc

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100419707B1 (en) * 1995-12-29 2004-06-12 씨제이 주식회사 Purification method of vancomycin using prep-hplc
KR100385152B1 (en) * 2001-03-14 2003-05-23 씨제이 주식회사 Process for the purification of capreomycin

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