KR930013133A - Method for Purifying 5'-inosinic Acid-Containing Liquid (I) - Google Patents

Method for Purifying 5'-inosinic Acid-Containing Liquid (I) Download PDF

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Publication number
KR930013133A
KR930013133A KR1019910024567A KR910024567A KR930013133A KR 930013133 A KR930013133 A KR 930013133A KR 1019910024567 A KR1019910024567 A KR 1019910024567A KR 910024567 A KR910024567 A KR 910024567A KR 930013133 A KR930013133 A KR 930013133A
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KR
South Korea
Prior art keywords
eluted
exchange resin
extruded
purifying
inosinic
Prior art date
Application number
KR1019910024567A
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Korean (ko)
Inventor
강기권
박한선
이계철
이승태
Original Assignee
유영학
주식회사 미원
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Priority to KR1019910024567A priority Critical patent/KR930013133A/en
Publication of KR930013133A publication Critical patent/KR930013133A/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

본 발명은 미생물 발효에 의하여 제조한 균체가 함유된 5'-이노신산 발효액을 정제함에 있어서, 발효액을 열처리하여 균체를 체거한 후 pH조절하여 강염기성 음이온 교환 수지에 흡착시켜 산으로 용리시키고, 용리된 액중 일부를 강산성 양이온 교환, 수지에 1차 흡착시켜 순수로 압출 및 용리한 후, 동일탑에 나머지 용리액을 흡착시키고 압출 및 용리과정을 거침으로써 불순물인 이온 물질 및 기타 핵산류를 다량 제거시키고, 기존 방법에 의한 용리액과 비교시 OD나 순도의 차이는 없으나 고동도인 5'-이노신산 용리액을 얻는 방법에 관한 것이다.The present invention is to purify the 5'-inosinic acid fermentation broth containing the cells produced by the microbial fermentation, heat treatment of the fermentation broth, the cells are collected and then adjusted to pH by adsorbing to a strong base anion exchange resin eluted with acid, eluted Part of the liquid is strongly acidic cation exchange, first adsorbed to the resin, extruded and eluted with pure water, and then the remaining eluent is adsorbed on the same tower and extruded and eluted to remove a large amount of impurities such as impurities and other nucleic acids. Compared with the eluent by the method, there is no difference in OD or purity, but it relates to a method of obtaining a highly dynamic 5'-inosinic eluate.

Description

5'-이노신산 함유액의 정제방법(I)Method for Purifying 5'-inosinic Acid-Containing Liquid (I)

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (1)

발효법에 의해 제조한 5'-이노신산 발효액으로 부터 균체를 분리한후 음이온 교환 수지 및 양이온 교환 수지에 흡착 및 용리시켜 5'-이노신산을 정제하는 방법에 있어서, 음이온 교환 수지로부터 용리시킨 용리액 중 일부를 강산성 양이온 교환 수지에 1차 흡착시켜 순수로 압출 및 용리한 후 나머지 용리액을 다시 동일한 강산성 양이온 교환 수지에 2차 흡착시키고 순수로 압출 및 용리함을 특징으로 하는 정제방법.In the method of purifying 5'-inosinic acid by separating the cells from the 5'-inosinic acid fermentation broth prepared by the fermentation method, and adsorbing and eluting the anion exchange resin and the cation exchange resin, a part of the eluate eluted from the anion exchange resin is removed. Purifying by primary adsorption to a strong acid cation exchange resin extruded and eluted with pure water, the remaining eluent is second adsorbed again to the same strong acid cation exchange resin and extruded and eluted with pure water. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019910024567A 1991-12-27 1991-12-27 Method for Purifying 5'-inosinic Acid-Containing Liquid (I) KR930013133A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019910024567A KR930013133A (en) 1991-12-27 1991-12-27 Method for Purifying 5'-inosinic Acid-Containing Liquid (I)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019910024567A KR930013133A (en) 1991-12-27 1991-12-27 Method for Purifying 5'-inosinic Acid-Containing Liquid (I)

Publications (1)

Publication Number Publication Date
KR930013133A true KR930013133A (en) 1993-07-21

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ID=67345772

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019910024567A KR930013133A (en) 1991-12-27 1991-12-27 Method for Purifying 5'-inosinic Acid-Containing Liquid (I)

Country Status (1)

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KR (1) KR930013133A (en)

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