KR930013120A - Transformed rice defoliated and virus resistant rice and its manufacturing method - Google Patents

Transformed rice defoliated and virus resistant rice and its manufacturing method Download PDF

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KR930013120A
KR930013120A KR1019920022980A KR920022980A KR930013120A KR 930013120 A KR930013120 A KR 930013120A KR 1019920022980 A KR1019920022980 A KR 1019920022980A KR 920022980 A KR920022980 A KR 920022980A KR 930013120 A KR930013120 A KR 930013120A
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rice
plant
gene
vector
sequence
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KR1019920022980A
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KR100255474B1 (en
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야펭 주
다까히꼬 하야가와
시게미쓰 도리야마
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오까노 미쓰다께
미쓰비시 쇼오지 가부시기가이샤
에도오 다께도시
미쯔비시 가세이 가부시기가이샤
아사가 오고이찌
노오린스이산쇼오 노오교오강꾜오기쥬쓰 겡규우쇼
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)

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Abstract

벼과 식물에서 기능하는 프로모우터의 하류에 벼 고엽고 바이러스 감염 특히 단백질을 코우드하는 유전자 및 터미네이트를 도입한 벡터로 벼를 형질 전환시키고 이 유전자를 발현시킴으로써 벼 고엽고 바이러스에 저항성을 가진 벼를 만든다. 이 방법을 사용하면 종래 공지되어 있는 벼 고엽고 바이러스 외피 단백질 이외의 비구조 단백질을 이용하여 이러한 바이러스 저항성의 벼를 만들 수가 있다.Downstream of the promoters that function in rice plants, they transform the rice with the vector that introduces the genes and terminus of the genes and terminus that infects the virus, especially the protein, and expresses the genes so that Make. Using this method, such viral resistant rice can be made using non-structural proteins other than the conventionally known rice foliar and viral coat proteins.

Description

형질전환된 벼 고엽고 바이러스 저항성 벼 및 그 제조방법Transformed rice defoliated and virus resistant rice and its manufacturing method

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 실시예에서 얻는 형질전환용 플라스미드 pLAN160의 모식도.1 is a schematic diagram of the transforming plasmid pLAN160 obtained in Example.

Claims (8)

벼과 식물에서 기능하는 프로모우터의 하류에 벼 고엽고 바이러스 감염 특이 단백질을 코우드하는 유전자 및 터미네이터를 도입하여 됨을 특징으로 하는 벡터.A vector characterized by introducing a gene and a terminator that are condensed with a rice foliar and viral infection specific protein downstream of a promoter functioning in a rice plant. 제1항에 있어서, 벼 고엽고 바이러스 감염 특이 단백질을 코우드하는 유전자가 배열표의 배열 번호1에 기재된 아미노산 배열로 나타내어지는 단백질을 코우드하는 유전자임을 특징으로 하는 벡터.The vector according to claim 1, wherein the gene which codes for rice foliar and viral infection specific protein is a gene which codes for the protein represented by the amino acid sequence of SEQ ID NO: 1 of the sequence table. 제2항에 있어서, 벼 고엽고 바이러스 감염 특이 단백질을 코우드하는 유전자 배열표의 배열 번호1에 기재된 염기 배열로 나타내어지는 것을 특징으로 벡터.The vector according to claim 2, which is represented by the nucleotide sequence set forth in SEQ ID NO: 1 of the gene sequence listing rice foliar and viral infection specific proteins. 제1항 내지 제3항에 기재된 벡터를 과 식물의 프로토플라스트에 도입하여 클로니를 형성시킨 후 이 콜로니로 부터 식물체를 재생시켜 얻은 벼과 식물.The rice plant obtained by regenerating a plant from this colony after introducing the vector of Claims 1-3 into the protoplast of a fruit plant, and forming a clone. 제1항 내지 제3항에 기재된 벡터를 벼의 프로토플라스트에 도입하여 콜로니를 형성시킨 후 이 콜로니로부터 식물체를 재생시켜 얻은 벼.A rice obtained by introducing a vector according to claims 1 to 3 into a prototype of rice to form colonies and then regenerating plants from the colonies. 제1항 내지 제3항에 기재된 벡터와 벼과 식물 유래의 프로플라스트를 액체 매체중에 현탁시켜 전기 펄스를 인가하여 이 벡터를 도입한 후, 벼 배양세포를 함유하는 배지에서 배양하여 콜로니를 형성시키고, 이 콜로니로부터 식물체를 재생시킴을 특징으로 하는 벼과 식물의 제조방법.The vector and the plant derived from rice plants in Suspended in Suspension was suspended in a liquid medium and an electric pulse was applied to introduce the vector, followed by culturing in a medium containing rice culture cells to form colonies. , A method of producing a rice plant, characterized in that to regenerate the plant from this colony. 배열표의 배열 번호1에 기재된 아미노산 배열로 나타내어지는 벼 고엽고 바이러스 감염 특이 단백질을 코우드하는 벼 고엽고 바이러스 게놈 RNA4 유전자.A rice conifer virus genomic RNA4 gene which codes for a rice coniferous and viral infection specific protein represented by the amino acid sequence of SEQ ID NO: 1 of the sequence table. 배열표의 배열 번호2에 기재된 염기 배열로 나타내어지는 벼 고엽고 바이러스 게놈 RNA4 유전자.The rice seedling virus genomic RNA4 gene represented by the nucleotide sequence of sequence number 2 of the sequence table. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019920022980A 1991-12-02 1992-12-01 Transformed rice resistant to rice stripe virus and its production KR100255474B1 (en)

Applications Claiming Priority (2)

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JP3318211A JPH07313173A (en) 1991-12-02 1991-12-02 Transformed rice resistant to rice stripe virus and its production
JP91-318211 1991-12-02

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KR930013120A true KR930013120A (en) 1993-07-21
KR100255474B1 KR100255474B1 (en) 2000-05-01

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KR (1) KR100255474B1 (en)
CN (1) CN1044385C (en)
TW (1) TW290426B (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100822742B1 (en) * 2007-03-19 2008-04-17 대한민국 Gene clone of recombinant groel originated from small brown planthopper and detection method of immunocapture rt-pcr for rice stripe virus using the groel protein
KR101066011B1 (en) 2008-09-23 2011-09-20 경상대학교산학협력단 Embryonic Callus Induction and Plant Regeneration Methods from Mature Seed of Miscanthus sinensis
KR101013092B1 (en) * 2009-03-25 2011-02-14 대한민국 Rice Stripe Virus Resistant Rice Transformants and Method of Producing Thereof
JP2011067115A (en) * 2009-09-24 2011-04-07 National Agriculture & Food Research Organization Viral gene for obtaining plant having resistance to virus belonging to tenuivirus by rna interference
CN109169280B (en) * 2018-09-20 2022-02-08 四川农业大学 Preparation method of coix seed regenerated plant and culture medium used by preparation method

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KR100255474B1 (en) 2000-05-01
CN1044385C (en) 1999-07-28
TW290426B (en) 1996-11-11
CN1075335A (en) 1993-08-18
JPH07313173A (en) 1995-12-05

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