KR930013101A - Method for preparing fructosyltransferase and β-galactosidase - Google Patents

Method for preparing fructosyltransferase and β-galactosidase Download PDF

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Publication number
KR930013101A
KR930013101A KR1019910023739A KR910023739A KR930013101A KR 930013101 A KR930013101 A KR 930013101A KR 1019910023739 A KR1019910023739 A KR 1019910023739A KR 910023739 A KR910023739 A KR 910023739A KR 930013101 A KR930013101 A KR 930013101A
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South Korea
Prior art keywords
galactosidase
fructosyltransferase
producing
carbon source
preparing
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KR1019910023739A
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Korean (ko)
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KR930008974B1 (en
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인만진
최경호
김민홍
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유영학
주식회사 미원
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Priority to KR1019910023739A priority Critical patent/KR930008974B1/en
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Publication of KR930008974B1 publication Critical patent/KR930008974B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

본 발명은 탄소원의 종류에 따라 프락토실트랜스퍼라제 또는 β-갈락토시다제를 생산할 수 있는 오레오바시듐 플루란스(Aureobasidium pullulans)MW-4218(KFCC 10754)을 탄소원으로 각각 설탕 또는 유당을 함유하는 영양배지에서 25~35℃, 중성pH에서 호기적으로 배양하여 균체내 또는 배양액중에 프락토실트랜스퍼라제 또는 β-갈락토시다제의 제조방법에 관한 것이다.According to the present invention, Aureobasidium pullulans MW-4218 (KFCC 10754) capable of producing fructosyltransferase or β-galactosidase according to the type of carbon source contains sugar or lactose, respectively. It relates to a method for producing fructosyltransferase or β-galactosidase in cells or culture by aerobic culture at 25 ~ 35 ℃, neutral pH in nutrient medium.

Description

프락토실트랜스퍼라제 및 β-갈락토시다제의 제조방법.Method for preparing fructosyltransferase and β-galactosidase.

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (3)

탄소원의 종류에 따라 프락토실트랜스퍼라제 또는 β-갈락토시다제를 생산할 수 있는 오레오바시듐 플루란스(Aureobasidium pullulans)MW-4218(KFCC 10754)을 탄소원으로 각각 설탕 또는 유당을 함유하는 영양배지에서 25~35℃, 중성 pH에서 호기적으로 배양하여 균체내 또는 배양액중에 프라톡실트랜스퍼라제 또는 β-갈락토시다제를 축적시킨후 그로부터 이들 효소를 희수함을 특징으로 하는 프락토실트랜스퍼라제 및 β-갈락토시다제의 제조방법.Aureobasidium pullulans MW-4218 (KFCC 10754), which can produce fructosyltransferase or β-galactosidase, depending on the type of carbon source, is used as a carbon source in a nutrient medium containing sugar or lactose, respectively. The fructosyltransferase and β characterized in that aerobic culture at 25-35 ° C., neutral pH to accumulate pratoxyltransferase or β-galactosidase in the cells or in the culture medium and then dilute these enzymes therefrom. Preparation of galactosidase. 제1항에 있어서, β-갈락토시다제를 생산하는 경우에는 β-갈락토시다제 생산 유도제인 이소프로필-β-티오갈락토퓨라노사이드(IPTG)를 2,0~10mM의 농도로 첨가함을 특징으로 하는 프락토실트랜스퍼라제 및 β-갈락토시다제의 제조방법.The method according to claim 1, wherein when producing β-galactosidase, isopropyl-β-thiogalactofuranoside (IPTG), which is a β-galactosidase production inducer, is added at a concentration of 2,0-10 mM. Method for producing fructosyl transferase and β-galactosidase characterized in that. 제1항에 있어서, 생산된 β-갈락토시다제의 전이활성이 가수분해 활성의 50%이상임을 특징으로 하는 프락토실트랜스퍼라제 및 β-갈락토시다제의 제조방법.The method for producing fructosyltransferase and β-galactosidase according to claim 1, wherein the produced β-galactosidase has a transition activity of 50% or more of the hydrolytic activity. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019910023739A 1991-12-21 1991-12-21 Method for preparation of fructosyl transferase & beta-galactosidase KR930008974B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019910023739A KR930008974B1 (en) 1991-12-21 1991-12-21 Method for preparation of fructosyl transferase & beta-galactosidase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019910023739A KR930008974B1 (en) 1991-12-21 1991-12-21 Method for preparation of fructosyl transferase & beta-galactosidase

Publications (2)

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KR930013101A true KR930013101A (en) 1993-07-21
KR930008974B1 KR930008974B1 (en) 1993-09-17

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KR1019910023739A KR930008974B1 (en) 1991-12-21 1991-12-21 Method for preparation of fructosyl transferase & beta-galactosidase

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