KR830005345A - Production method of penicillin amidase - Google Patents

Production method of penicillin amidase Download PDF

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Publication number
KR830005345A
KR830005345A KR1019810001413A KR810001413A KR830005345A KR 830005345 A KR830005345 A KR 830005345A KR 1019810001413 A KR1019810001413 A KR 1019810001413A KR 810001413 A KR810001413 A KR 810001413A KR 830005345 A KR830005345 A KR 830005345A
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KR
South Korea
Prior art keywords
penicillin amidase
production method
phenylacetic acid
amidase
producing penicillin
Prior art date
Application number
KR1019810001413A
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Korean (ko)
Other versions
KR830001800B1 (en
Inventor
한문희
민태익
손형진
성백린
Original Assignee
이주천
한국과학기술원
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Publication date
Application filed by 이주천, 한국과학기술원 filed Critical 이주천
Priority to KR1019810001413A priority Critical patent/KR830001800B1/en
Publication of KR830005345A publication Critical patent/KR830005345A/en
Application granted granted Critical
Publication of KR830001800B1 publication Critical patent/KR830001800B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음No content

Description

페니실린아미다제의 생산방법Production method of penicillin amidase

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (1)

페니실린 아미다제를 생산함에 있어서, 부분적 항상성 변이주인 바실루스 메가테리움(KFCC 10029)을 표 2의 배지에서 배양한 다음, 이들 1.5-2.5VVM의 통기량과 350-550rpm의 교반속도하에서 10% 비율로 접종하고 32-35℃에서 8시간 배양한 후 다시 28℃-32℃로 낮추면서 0.3%의 페닐아세트산을 가하고 다시 12시간 후에 0.3%의 페닐아세트산을 가하는 것을 특징으로 하는 페니실린 아미다제의 생산 방법.In producing penicillin amidase, the partial homeostasis strain Bacillus megaterium (KFCC 10029) was incubated in the medium of Table 2, followed by aeration rate of 1.5-2.5VVM and a stirring rate of 350-550 rpm at 10%. Inoculating and incubating at 32-35 ° C. for 8 hours, then lowering to 28 ° C.-32 ° C., adding 0.3% of phenylacetic acid and again 12% after adding 0.3% of phenylacetic acid, the method of producing penicillin amidase. 표2. 배지 조성Table 2. Badge composition ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019810001413A 1981-04-24 1981-04-24 Method for manufacturing penicillin amidase KR830001800B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019810001413A KR830001800B1 (en) 1981-04-24 1981-04-24 Method for manufacturing penicillin amidase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019810001413A KR830001800B1 (en) 1981-04-24 1981-04-24 Method for manufacturing penicillin amidase

Publications (2)

Publication Number Publication Date
KR830005345A true KR830005345A (en) 1983-08-13
KR830001800B1 KR830001800B1 (en) 1983-09-08

Family

ID=19220795

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019810001413A KR830001800B1 (en) 1981-04-24 1981-04-24 Method for manufacturing penicillin amidase

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KR (1) KR830001800B1 (en)

Also Published As

Publication number Publication date
KR830001800B1 (en) 1983-09-08

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