KR960017841A - Method for producing a culture plate for screening microorganisms producing sugar transferase - Google Patents

Method for producing a culture plate for screening microorganisms producing sugar transferase Download PDF

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Publication number
KR960017841A
KR960017841A KR1019940031865A KR19940031865A KR960017841A KR 960017841 A KR960017841 A KR 960017841A KR 1019940031865 A KR1019940031865 A KR 1019940031865A KR 19940031865 A KR19940031865 A KR 19940031865A KR 960017841 A KR960017841 A KR 960017841A
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South Korea
Prior art keywords
producing
medium composition
culture plate
culture dish
screening
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KR1019940031865A
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Korean (ko)
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KR0131418B1 (en
Inventor
이원희
김정수
이영철
이병연
서병철
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성우경
두산기술원 연구조합
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Publication of KR960017841A publication Critical patent/KR960017841A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 공지의 미생물 배지조성에 적량의 p-니트로페닐글루코피라노시드(p-NPG)와 피리딘을 첨가함을 특징으로 하는 당전이 효소 생성 미생물 선별용 배양접시의 제조방법에 관한 것이다.The present invention relates to a method for preparing a culture plate for selecting a sugar transfer enzyme-producing microorganism, comprising adding an appropriate amount of p-nitrophenylglucopyranoside (p-NPG) and pyridine to a known microorganism medium composition.

Description

당전이 효소를 생성하는 미생물 선별용 배양접시의 제조방법Method for producing a culture plate for screening microorganisms producing sugar transferase

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (5)

공지의 미생물 배지조성에 적량의 p-니트로페닐글루코피라노시드(p-NPG)와 피리딘을 첨가함을 특징으로 하는 당전이 효소 생성 미생물 선별용 배양접시의 제조방법A method for preparing a culture plate for screening a sugar transferase-producing microorganism, comprising adding an appropriate amount of p-nitrophenylglucopyranoside (p-NPG) and pyridine to a known microorganism medium composition. 제1항에 있어서, 공지의 미생물 배지조성은 MEA 배지 조성 또는 Czapek 배지조성임을 특징으로 하는 당전이 효소 생성 미생물 선별용 배양접시의 제조방법.The method of claim 1, wherein the known microbial medium composition is a MEA medium composition or a Czapek medium composition. 제1항에 있어서, 배지조성내의 p-NPG의 농도는 3~7mM이고, 피리딘의 농도는 20~30mM임을 특징으로 하는 당전이 효소 생성 미생물 선별용 배양접시의 제조방법.The method of claim 1, wherein the concentration of p-NPG in the medium composition is 3 ~ 7mM, the pyridine concentration is 20 ~ 30mM method of producing a culture plate for selecting a sugar transfer enzyme-producing microorganism. 제1항의 방법에 따라 제조된 당전이 효소 생성 미생물 선별용 배양접시.A culture dish for selecting a sugar transfer enzyme-producing microorganism prepared according to the method of claim 1. 제1항의 방법에 따라 제조된 배양접시에 미생물을 배양시킨후, 배양접시에 적량의 탄산나트륨용액을 첨가하여, 배양접시에 나타나는 황색 환의 유무와 크기, 색감등을 관찰함을 특징으로 하는 당전이 효소 생성 미생물의 선별방법.After culturing the microorganisms in the culture dish prepared according to the method of claim 1, a suitable amount of sodium carbonate solution is added to the culture dish to observe the presence, size, color, etc. of the yellow ring appearing in the culture dish. Screening method of produced microorganisms. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940031865A 1994-11-30 1994-11-30 Process for preparing selective media for transglucosidase producing microorganism Expired - Fee Related KR0131418B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940031865A KR0131418B1 (en) 1994-11-30 1994-11-30 Process for preparing selective media for transglucosidase producing microorganism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940031865A KR0131418B1 (en) 1994-11-30 1994-11-30 Process for preparing selective media for transglucosidase producing microorganism

Publications (2)

Publication Number Publication Date
KR960017841A true KR960017841A (en) 1996-06-17
KR0131418B1 KR0131418B1 (en) 1998-04-11

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019940031865A Expired - Fee Related KR0131418B1 (en) 1994-11-30 1994-11-30 Process for preparing selective media for transglucosidase producing microorganism

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KR0131418B1 (en) 1998-04-11

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