KR930012804A - Method for separating and purifying polysaccharides from ginkgo biloba - Google Patents

Method for separating and purifying polysaccharides from ginkgo biloba Download PDF

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Publication number
KR930012804A
KR930012804A KR1019910025482A KR910025482A KR930012804A KR 930012804 A KR930012804 A KR 930012804A KR 1019910025482 A KR1019910025482 A KR 1019910025482A KR 910025482 A KR910025482 A KR 910025482A KR 930012804 A KR930012804 A KR 930012804A
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KR
South Korea
Prior art keywords
ginkgo biloba
separating
polysaccharide
fat
purifying
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Application number
KR1019910025482A
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Korean (ko)
Inventor
안정갑
곽의종
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이승동
주식회사 선경인더스트리
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Priority to KR1019910025482A priority Critical patent/KR930012804A/en
Publication of KR930012804A publication Critical patent/KR930012804A/en

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Abstract

본 발명은 은행엽으로부터 다당체를 분리, 정제하는 방법에 관한 것으로서, 더욱 상세하게는 은행엽 열수추출액으로부터 생체유효활성을 검색하기 위해 다당체를 분리 정제하는 방법에 관한 것이다.The present invention relates to a method for separating and purifying polysaccharides from ginkgo biloba, and more particularly, to a method for separating and purifying polysaccharides to search for bioactive activity from ginkgo biloba hot water extract.

Description

은행엽으로부터 다당체를 분리 정제하는 방법Method for separating and purifying polysaccharides from ginkgo biloba

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 본 발명에 따른 실시예 1에서 실시한 겔여과크로마토그래피도,1 is a gel filtration chromatography carried out in Example 1 according to the present invention,

제2도는 본 발명에 따른 실시예 1에서 얻어진 다당체의 IR도이다.2 is an IR diagram of the polysaccharide obtained in Example 1 according to the present invention.

Claims (4)

은행엽으로부터 다당체를 분리함에 있어서, 건조 은행엽을 열수추출한 후 이 추출액을 물과 혼화되지 않는 지용성 유기용매로 처리하여 단백질과 비극성 불순물을 제거하고 난 여액에 저급알콜을 가하여 생성된 침전물을 원심 분리하는 것을 특징으로 하는 은행엽으로 부터 다당체를 분리하는 방법.In separating the polysaccharide from the ginkgo biloba, the dried ginkgo biloba was hydrothermally extracted, and the extract was treated with a fat-soluble organic solvent that was incompatible with water to remove proteins and nonpolar impurities, and then centrifuged the precipitate formed by adding lower alcohol to the filtrate. A method for separating polysaccharides from ginkgo biloba, characterized in that. 제1항에 있어서, 상기 지용성 유기용매로는 클로로포름과 부탄을 혼합액을 사용함을 특징으로 하는 다당체의 분리방법.The method of claim 1, wherein the fat-soluble organic solvent is a separation method of the polysaccharide, characterized in that a mixture of chloroform and butane. 제1항에 있어서, 상기 저급 알콜로는 에칠알콜을 사용함을 특징으로 하는 다당체의 분리방법.2. The method of claim 1, wherein the lower alcohol is used as ethanol. 은행엽으로부터 다당체를 분리, 정제함에 있어서, 건조은행엽을 열수 추출한 후 이 추출액을 물과 혼화되지 않는 지용성 유기용매로 처리하여 단백질과 비극성 불순물을 제거하고, 여액에 저급알콜을 적가하여 생성된 침전물을 원심분리한 다음 겔여과크로마토그래피를 이용하여 두 종류의 분자군으로 구성된 다당체를 정제하는 것을 특징으로 하는 은행엽으로부터 다당체를 분리, 정제하는 방법.In separating and purifying polysaccharides from ginkgo biloba, dried ginkgo biloba was extracted with hot water, and the extract was treated with a fat-soluble organic solvent that was incompatible with water to remove proteins and nonpolar impurities, and a precipitate formed by dropping low alcohol into the filtrate. Method for separating and purifying the polysaccharide from the ginkgo biloba, characterized in that after centrifugation to purify the polysaccharide consisting of two kinds of molecular groups using gel filtration chromatography. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019910025482A 1991-12-30 1991-12-30 Method for separating and purifying polysaccharides from ginkgo biloba KR930012804A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019910025482A KR930012804A (en) 1991-12-30 1991-12-30 Method for separating and purifying polysaccharides from ginkgo biloba

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019910025482A KR930012804A (en) 1991-12-30 1991-12-30 Method for separating and purifying polysaccharides from ginkgo biloba

Publications (1)

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KR930012804A true KR930012804A (en) 1993-07-21

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