KR930004763A - 중합체 분석방법 - Google Patents

중합체 분석방법 Download PDF

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KR930004763A
KR930004763A KR1019920015529A KR920015529A KR930004763A KR 930004763 A KR930004763 A KR 930004763A KR 1019920015529 A KR1019920015529 A KR 1019920015529A KR 920015529 A KR920015529 A KR 920015529A KR 930004763 A KR930004763 A KR 930004763A
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polymer
nucleic acid
molecular weight
low molecular
antigen
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KR1019920015529A
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시게루 미와
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시게루 미와
가부시끼가이샤 이무노바이온
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Publication of KR930004763A publication Critical patent/KR930004763A/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/538—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip, i.e. sorbent materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

내용 없음.

Description

중합체 분석방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (22)

  1. 중합체와 저분자물질을 함유한 시료로부터 분석 목표 중합체를 분리해내는 방법에 있어서, 상기 시료에 상기 분석목표중합체와 같거나 또는 다른 중합체가 결합된 불용성 담체를 접촉시켜 실질상 포화시켜서, 상기 저분자 물질을 상기 불용성 담체상에 흡착시킴으로써 상기 담체에 비흡착된 분석목표 중합체를 시료로부터 분리함을 특징으로 하는 중합체 분리 방법.
  2. 저분자 물질이 분석목표중합체에 특이적으로 결합되었는지 여부를 판정하는 방법에 있어서, 분석목표 중합체와 저분자물짙을 같거나 또는 다른 중합체가 미리 결합된 불용성 담제에 접촉시켜 실질상 포화시키고, 상기 불용성 담체에 비흡착된 저분자물질의 양을 분석함으로써, 상기 분석 목표중합체에 특이적으로 결합된 저분자물질의 양을 구함을 특징으로 하는 판정방법.
  3. 제2항에 있어서, 상기 불용성 담체에 결합된 중합체가 생물에서 얻어진 중합체 또는 합성물인 것이 특징인 판정방법.
  4. 제3항에 있어서, 상기 생물에서 얻은 중합체가 핵산인 것이 특징인 판정방법.
  5. 제4항에 있어서, 상기 핵산이 DNA인 것이 특징인 판정방법
  6. 제2항에 있어서, 상기 불용성 담체가 흡착제인 것이 특징인 판정방법.
  7. 제6항에 있어서, 상기 흡착제가 활성알루미나 또는 활성목탄인 것이 특징인 판정방법.
  8. 제2항에 있어서, 상기 저분자물질의 분자량이 상기 분석목표중합체보다 분자량이 더 낮은 것이 특징인 판정방법.
  9. 제2항 또는 제8항에 있어서, 상기 저분자 물질이 올리고뉴클레오티드, 폴리뉴클레오티드 및 단백질중 적어도 하나를 함유하는 것이 특징인 판정방법.
  10. 제9항에 있어서, 상기 올리고뉴클레오티드 또는 폴리뉴플레오티드가 혼성화용으로 사용되는 표지된 프로브인 것이 특징인 판정방법.
  11. 제9항에 있어서, 상기 단백질이 표지된 항체인 것이 특징인 판정방법.
  12. 제2항 또는 제11항에 있어서, 상기 분석목표 중합체가 핵산인 것이 특징인 판정방법.
  13. 제12항에 있어서, 핵산중 염기수가 1,000 염기이상인 것이 특징인 판정방법.
  14. 제13항에 있어서, 상기 핵산이 항원 또는 리간드와 결합된 것이 특징인 판정방법.
  15. 항원, 이와 동일한 항원과 결합된 핵산 및 표지된 항체의 혼합물을 중합체와 결합된 불용성 담체와 접촉시켜 실질성 포화시키고, 상기 불용성 담체에 비흡착된 표지된 항체의 양을 분석하는 것이 특징인 항원량 측정방법.
  16. 제2항에 있어서, 상기 분석 목표 중합체가 핵산이고, 저분자 물질이 상기 핵산에 대한 올리고뉴를로오티드 프로브인 것이 특징인 판정방법.
  17. 제16항에 있어서, 표지된 항체를 갖는 프로브에 결합된 항원을 항원에 결합시키고, 상기 볼용성 담체에 비흡착된 표지된 항체의 양을 측정함으로서, 상기 핵산에 결합된 프로브의 양을 측정하는 것이 특징인 판정방법.
  18. 제17항에 있어서, 상기 항원이 헵텐인 것이 특징인 판정방법.
  19. 제17항에 있어서, 상기 표지된 항체가, 효소, 형광물질, 발광물질, 염료, 조효소, 헵텐, 방사성 동위원소, 또는 효소순환 가능한 기질중 어느 하나인 것이 특징인 판정방법.
  20. 제19항에 있어서, 상기 효소가 트리오스 포스페이트 이성화제, 퍼옥시다제, 알칼린 포스파타제, 애시드 포스파타제 및 β-갈락토시다제중에서 선택된 것이 특징인 판정방법.
  21. 제21항에 있어서, 상기 분석목표핵산이 효소반응용 기질 또는 템플레이트로서 작용하는 것이 특징인 판정방법.
  22. 제21항에 있어서 상기 효소가 DNA폴리머라제, RAN폴리머라제, 말단 전이효소, 폴리뉴클레오티드 키나제, 뉴클레마제 S1 및 리가제 중에서 선택된 것이 특징인 판정방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
KR1019920015529A 1991-08-30 1992-08-28 중합체 분석방법 KR930004763A (ko)

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Application Number Priority Date Filing Date Title
JP91-300002 1991-08-30
JP3300002A JPH0560761A (ja) 1991-08-30 1991-08-30 重合体の分析方法

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JP (1) JPH0560761A (ko)
KR (1) KR930004763A (ko)
AU (1) AU2121492A (ko)
CA (1) CA2077157A1 (ko)

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EP0920535B1 (de) * 1996-08-26 2007-11-14 Invitek Gesellschaft für Biotechnik & Biodesign mbH. Verfahren zum nachweis klinisch relevanter veränderungen der dns-sequenz des ki-ras-onkogens, seine verwendung und testkit zur früherkennung von tumoren
EP1469067A4 (en) * 2002-01-25 2007-05-23 Olympus Corp METHOD AND SYSTEM FOR DETECTING DATA RELATING TO A NUCLEIC ACID
US20060243658A1 (en) * 2002-11-08 2006-11-02 Zubov Vitali P Sorbent material having a covalently attached perfluorinated surface with functional groups

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* Cited by examiner, † Cited by third party
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US3442819A (en) * 1965-03-26 1969-05-06 Mount Sinai Hospital Research Molecular sieve coated particulate adsorbent and processes using same
US3958944A (en) * 1974-07-15 1976-05-25 Wong Johnson N S Vial assembly
US4348374A (en) * 1977-09-16 1982-09-07 Abbott Laboratories Charcoal coated adsorbent device
CA1309672C (en) * 1983-01-27 1992-11-03 Jannis G. Stavrianopoulos Methods and structures employing non-radioactive chemically-labeled polynucleotide probes
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays
AU619170B2 (en) * 1987-01-09 1992-01-23 Abbott Laboratories Diagnostic assays using nucleic acid probes
CA2011929A1 (en) * 1989-04-03 1990-10-03 Rex M. Bitner Metal oxide supports for nucleic acids

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CA2077157A1 (en) 1993-03-01
JPH0560761A (ja) 1993-03-12
AU2121492A (en) 1993-03-04
EP0535376A1 (en) 1993-04-07

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