KR930002508A - Method for preparing 5'-guanylic acid by recombinant microorganism - Google Patents

Method for preparing 5'-guanylic acid by recombinant microorganism Download PDF

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Publication number
KR930002508A
KR930002508A KR1019910013227A KR910013227A KR930002508A KR 930002508 A KR930002508 A KR 930002508A KR 1019910013227 A KR1019910013227 A KR 1019910013227A KR 910013227 A KR910013227 A KR 910013227A KR 930002508 A KR930002508 A KR 930002508A
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KR
South Korea
Prior art keywords
producing bacteria
gene cloned
ammonia genes
xanthyl acid
gmp
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Application number
KR1019910013227A
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Korean (ko)
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KR970005914B1 (en
Inventor
한종권
이현환
정성오
현형환
Original Assignee
김정순
제일제당 주식회사
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Priority to KR1019910013227A priority Critical patent/KR970005914B1/en
Publication of KR930002508A publication Critical patent/KR930002508A/en
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Publication of KR970005914B1 publication Critical patent/KR970005914B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

Abstract

내용 없음.No content.

Description

유전자 재조합 미생물에 의한 5′-구아닐산의 제조방법Method for preparing 5′-guanylic acid by recombinant microorganism

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 pGH16의 제한 효소 Sal I, Xba I, Pst I의 절단 지도 및 그의 형성 단계를 나타낸 것이다.Figure 1 shows the cleavage map of the restriction enzymes Sal I, Xba I, Pst I of pGH16 and its formation steps.

Claims (4)

브레비박테리움 암모니아게네스로 부터 클로닝된 5′-크산틸산 아미나제 유전자.5′-Xanthyl acid aminase gene cloned from Brevibacterium ammonia genes. 브레비박테리움 암모니아게네스로 부터 클로닝된 5′-크산틸산 아미나제 유전자를 함유하고 있는 코리네형 글루타메이트 생산 박테리아 및 에쉐리시아 속의 균주에서 형질전환 및 발현이 가능한 셔틀벡터 pGH 16.Shuttle vector pGH capable of transformation and expression in coryneform glutamate producing bacteria and strains of the genus Escherichia containing 5′-xanthyl acid aminase gene cloned from Brevibacterium ammonia genes. 브레비박테리움 암모니아게네스로 부터 클로닝된 5′-크산틸산 아미나제 유전자를 함유하고 있는 코리네형 글루타메이트 생산 박테리아 및 대장균에서 복제 및 발현이 가능한 발현 비히클 pGH 16으로 형질전환된 코리네형 글루타메이트 생산 박테리아 및 대장균.Coryneform glutamate producing bacteria containing the 5′-xanthyl acid aminase gene cloned from Brevibacterium ammonia genes and coryneform glutamate producing bacteria transformed with the expression vehicle pGH 16 capable of replication and expression in E. coli and Escherichia coli. 브레비박테리움 암모니아게네스로 부터 클로닝된 5′-크산틸산 아미나제 유전자를 함유하고 있는 코리네형 글루타메이트 생산 박테리아 또는 대장균을 배양배지에 배양하여, XMP를 GMP로 전환시키고, 그로부터 축적된 GMP를 회수함을 특징으로 하는 GMP의 제조방법.Coryneform glutamate-producing bacteria or Escherichia coli containing 5′-xanthyl acid aminase gene cloned from Brevibacterium ammonia genes were cultured in culture medium to convert XMP to GMP and recover GMP accumulated therefrom. GMP manufacturing method characterized in that. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019910013227A 1991-07-31 1991-07-31 Process for gmp by recombinant microorganism KR970005914B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019910013227A KR970005914B1 (en) 1991-07-31 1991-07-31 Process for gmp by recombinant microorganism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019910013227A KR970005914B1 (en) 1991-07-31 1991-07-31 Process for gmp by recombinant microorganism

Publications (2)

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KR930002508A true KR930002508A (en) 1993-02-23
KR970005914B1 KR970005914B1 (en) 1997-04-22

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KR1019910013227A KR970005914B1 (en) 1991-07-31 1991-07-31 Process for gmp by recombinant microorganism

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8309329B2 (en) 2008-02-25 2012-11-13 Ajinomoto Co., Inc. Process for production of 5′-guanylic acid

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8309329B2 (en) 2008-02-25 2012-11-13 Ajinomoto Co., Inc. Process for production of 5′-guanylic acid

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Publication number Publication date
KR970005914B1 (en) 1997-04-22

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