KR850007271A - Method for producing penicillin acylase by genetic engineering - Google Patents

Method for producing penicillin acylase by genetic engineering Download PDF

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Publication number
KR850007271A
KR850007271A KR1019840001756A KR840001756A KR850007271A KR 850007271 A KR850007271 A KR 850007271A KR 1019840001756 A KR1019840001756 A KR 1019840001756A KR 840001756 A KR840001756 A KR 840001756A KR 850007271 A KR850007271 A KR 850007271A
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South Korea
Prior art keywords
penicillin acylase
plasmid
gene
strain
acylase
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KR1019840001756A
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Korean (ko)
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KR860000348B1 (en
Inventor
장이섭(외2)
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신동관
태평양화학공업 주식회사
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Application filed by 신동관, 태평양화학공업 주식회사 filed Critical 신동관
Priority to KR1019840001756A priority Critical patent/KR860000348B1/en
Priority to JP59192737A priority patent/JPS60210985A/en
Priority to DE19843437224 priority patent/DE3437224A1/en
Publication of KR850007271A publication Critical patent/KR850007271A/en
Application granted granted Critical
Publication of KR860000348B1 publication Critical patent/KR860000348B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음No content

Description

유전자 조작에 의한 페니실린 지 아실라제의 제조방법Method for producing penicillin acylase by genetic engineering

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (5)

공여균주로 부터 제한효소를 이용하여 페니실린지 아실라제의 유전자를 분리하고, 동일 제한효소로 처리된 플라스미드에 접합시켜 재조합플라스미드를 만들어 이것을 이용하여 균주를 형질변환시켜 얻은 유전자 조작된 새로운 균주(KFCC-84-3)를 공지의 방법으로 배양하여 유전자 조작에 의한 페니실린 지 아실라제의 제조방법.Genetically modified new strain (KFCC-) obtained by separating the gene of penicillin acylase from the donor strain using a restriction enzyme, conjugating to a plasmid treated with the same restriction enzyme, and making a recombinant plasmid. 84-3) is a method for producing penicillin g acylase by culturing by a known method. 제1항에 있어서, 페니실린지 아실라제의 유전자의 공여균주로 페니실린지 아실라제의 유전자를 함유하는 에세리키아 콜리를 이용하는 유전자 조작에 의한 페니실린지 아실라제의 제조방법.2. The method for producing penicillin acylase according to claim 1, wherein the gene is used by using an Escherichia coli containing the penicillin acylase gene as a donor strain of the gene of the penicillin acylase. 제1항에 있어서, 플라스미드는 제한효소 EcoR1과 Pst1로 절단하는 유전자 조작에 의한 페니실린지 아실라제의 제조방법.The method for producing penicillin acylase according to claim 1, wherein the plasmid is cleaved with restriction enzymes EcoR 1 and Pst 1 . 제1항에 있어서, 제한효소로 절단된 디엔에이와 플라스미드를 연결효소로 접합시켰을 때 플라스미드, 바이러스, 크스미드(Cosmid)로써 균주의 형질변환을 일으키는 재조합플라스미드를 만드는 유전자 조작에 의한 페니실린지 아실라제의 제조방법.2. The penicillin acylase according to claim 1, wherein the DNA of the restriction enzyme and the plasmid conjugated with the ligase are conjugated with a plasmid to produce a recombinant plasmid that transforms the strain into plasmids, viruses, and cosmids. Manufacturing method. 제1항에 있어서, 유전자 조작된 새로운 균주라함은 페니실린지 아실라제의 생합성을 억제하는 유전자를 갖고 있지 않은 에세리키아 콜리에 재조합플라스미드를 형질전환시켜 얻는 균주를 말하는 유전자 조작에 의한 페니실린지 아실라제의 제조방법.The method of claim 1, wherein the new genetically engineered strain refers to a strain obtained by transforming an Escherichia coli recombinant plasmid that does not have a gene that inhibits the biosynthesis of penicillin acylase. Manufacturing method. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019840001756A 1984-04-03 1984-04-03 Method of penicillin acylase by gene manipulation process KR860000348B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
KR1019840001756A KR860000348B1 (en) 1984-04-03 1984-04-03 Method of penicillin acylase by gene manipulation process
JP59192737A JPS60210985A (en) 1984-04-03 1984-09-17 Production of penicillin g acylase by gene operation
DE19843437224 DE3437224A1 (en) 1984-04-03 1984-10-10 Process for the preparation of penicillin G acylase by fermentation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019840001756A KR860000348B1 (en) 1984-04-03 1984-04-03 Method of penicillin acylase by gene manipulation process

Publications (2)

Publication Number Publication Date
KR850007271A true KR850007271A (en) 1985-12-02
KR860000348B1 KR860000348B1 (en) 1986-04-12

Family

ID=19233427

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019840001756A KR860000348B1 (en) 1984-04-03 1984-04-03 Method of penicillin acylase by gene manipulation process

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JP (1) JPS60210985A (en)
KR (1) KR860000348B1 (en)
DE (1) DE3437224A1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2930794C2 (en) * 1979-07-28 1983-02-10 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Production of microorganisms for the cleavage or synthesis of β-lactam antibiotics

Also Published As

Publication number Publication date
JPS60210985A (en) 1985-10-23
DE3437224A1 (en) 1985-10-10
KR860000348B1 (en) 1986-04-12

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